39 results about "Lotus corniculatus" patented technology

The invention relates to rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing comparative genomics, relates to knowledge of subjects such as plant comparative genomics, genetics and bioinformatics, and belongs to the technical field of plant biotechnology. The rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing the comparative genomics mainly includes the steps of firstly, downloading full genomic sequences of Lotus corniculatus and collecting MLO (mildew resistance locus o) genes; secondly, identifying the MLO genes; thirdly, identifying phylogenetic relationship of the MLO genes; and fourthly comparing the MLO powdery mildew resistance genes. By the rapid identification, mining cycle of the powdery mildew resistance genes is shortened, and the powdery mildew resistance genes can be identified quickly. Corresponding co-separation functional marks (SNP (single nucleotide polymorphism), Scar and the like) can be developed through candidate powdery mildew resistance genes identified, the rapid identification is also available for molecular marker-assisted selection of the powdery mildew resistance genes, and accuracy is high. The rapid identification can also be used with other molecular markers for resistance genes to create multiresistance breeding materials, and accordingly breeding period is shortened, breeding efficiency is improved, and basis for elaborating powdery mildew resistance Lotus corniculatus molecular mechanisms is laid.

The invention discloses a method for repairing soil polluted by lead and cadmium. The method comprises following steps: a, at first, sampling polluted soil, and after sampling, carrying out detectionand analysis to obtain the contents of lead and cadmium; b, preparing a catalytic reaction solution, pouring the prepared purifying solution into a solvent bottle, and evenly spraying the solution onpolluted soil; c, preparing a mixture of inorganic substances and organic mushrooms, fully mixing, spraying the mixture on polluted soil, wherein for each mu, 45 kilograms of mixture are used; d, planting inedible plants on the soil, wherein the plants are one or more of Anaphalis flavescens, lotus corniculatus, plantain, cabbage, Erianthus rufipilus, Equisetum ramosissimum, Cyperus rotundus, Cineraria repanda, Saussurea japonica, and Pteris cretica; and e, periodically spraying weak phosphoric acid with a pH value of 6.8 or so on the polluted soil, wherein the spraying period is 2 to 4 days,and the spraying amount is 2 L / mu. Chemical means and biological means are combined to comprehensively process polluted soil, and the purifying effect is good.

The invention discloses microorganism fermented organic fertilizer and a preparation method thereof. After lotus corniculatus is cut, cattle, horses and sheep are fed with fresh grass or dry grass; the lotus corniculatus accounts for 50 percent or more of the total food weight of the cattle, the horses and the sheep; lotus corniculatus plants are partially decomposed and crushed when the cattle, the horses and the sheep ruminate and repeatedly chew the lotus corniculatus plants and the lotus corniculatus plants are fermented in stomachs, and then excrement is formed; the excrement is mixed with mixed straws, sawdust, plant ash and canteen waste dreg; then the materials are stacked together and are subjected to first-time fermentation; after the first-time fermentation is finished, fermented matters are turned; a microorganism nutrient solution and crushed lotus corniculatus root stalks are added layer by layer in a turning process and are subjected to secondary fermentation; a mixtureis turned for one time every other 3 to 10 days, so as to prepare the microorganism organic fertilizer. By adopting the organic fertilizer, the problem that underground insects are easy to hatch in afermentation and composting process of the biological organic fertilizer can be solved; meanwhile, after the fertilizer is applied, the breeding and reproduction capability of the underground insectsof a fertilized land can be inhibited, so that the problem of soil insect pests is radically solved, self-balance of soil is regulated and controlled and the self-recovery capability of the soil is improved.

The invention discloses a lotus corniculatus AP2 / ERF transcription factor and an encoding gene and application thereof and belongs to the field of AP2 / ERF transcription factors and application. The invention discloses the gene of an AP2 / ERF transcription factor separated from lotus corniculatus. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The amino acid sequence of protein encoded by the gene of the transcription factor is shown as SEQ ID NO.2. The invention further discloses an AP2 / ERF transcription factor structural domain which can be combined with a cis-acting element to start adverse-resistant response gene expression, and the amino acid sequence of the structural domain is shown as SEQ ID NO.3. Function conversion experiments prove that the gene of the AP2 / ERF transcription factor in a plant can improve salt tolerance of the transgenic plant remarkably, and it shows that the protein encoded by the gene of the transcription factor has a function of the AP2 / ERF transcription factor. The gene of the AP2 / ERF transcription factor has an important application prospect in improving resistance of the plant to adversity stress.

The invention belongs to the technical field of cultivation, and in particular relates to a method for raising chickens in a brier grape forest. According to the method, an ecological breeding way for planting brier grapes and raising the chickens is realized by the four steps of setting the grape forest, building a chicken house, carrying out stocking management and carrying out disease prevention and treatment; the faeces excreted by the chickens in a stocking process can be used as fertilizer of the grapes, so that the plant diseases and insect pests of the grapes are reduced; furthermore, the chickens also can eat lotus corniculatus or green bristle grass, insects, fallen leaves and other materials, so that the feed source of the chickens is enriched, and the feed consumption is reduced; furthermore, after the method is adopted, the growth and development of the chickens can be promoted, and the meat quality of the chickens is improved; after the feed is matched for use, an ecological and environment-friendly cultivation mode is realized.

The invention relates to the technical field of skin care products, and particularly discloses a sodium hyaluronate moisturizing serum and a preparation method thereof. The sodium hyaluronate moisturizing serum comprises the following ingredients: water, mannitol, glycyrrhiza glabra root extracts, humectant, moisturizing accelerant and viscosity regulating agent, wherein the humectant comprises sodium hyaluronate, trehalose and [beta]-glucan; the moisturizing accelerant comprises the following ingredients: codium tomentosum extracts, tremella fuciformis Berk., polyglutamic acid, camellia seedoil and red algae extracts; and the viscosity regulating agent is prepared by mixing sodium polyacrylate, foeniculum vulgare extracts and phytosterol. The preparation method for the sodium hyaluronatemoisturizing serum comprises the following steps of: adding Carbomer 940, the humectant, the moisturizing accelerant, preservative and skin regulating agent into water, and adding onion skin powders,lotus corniculatus L extracts, the mannitol, the glycyrrhiza glabra root extracts, glycine betaine, ethylhexylglycerin and the viscosity regulating agent. The sodium hyaluronate moisturizing serum disclosed by the invention has the advantages of a good water supplementing effect and a long-lasting moisturizing effect, is clear and is not greasy after being smeared.

The invention discloses a method for preparing a lotus corniculatus feed through microorganism fermentation. The method comprises the following steps of (1) adding a nutritive salt solution to crushedlotus corniculatus according to the material-liquid ratio of 1-5ml / g, performing sterilization under the sealed condition, and then performing cooling to room temperature so as to obtain raw materialliquid; (2) according to the inoculum concentration that every 100g of the lotus corniculatus is inoculated with 4-16ml of bacterial liquid, adding aspergillus oryzae liquid of which the spore concentration is 10<6>-10<7> / mL to the raw material liquid, performing placing in a constant-temperature shaking culture box of 26-32 DEG C, and performing fermentation and culturing for 3-10 days; and (3)sterilizing fermentation products obtained in the step (2), then adding water, performing water extraction at 95+ / -10 DEG C for 1+ / -0.5h, wherein the material-liquid ratio of the lotus corniculatus tothe water is 1g to (8-12)ml, performing solid-liquid separation on substances obtained through water extraction, and drying residues so as to obtain the lotus corniculatus feed. Through adoption of the method disclosed by the invention, the protein hydrolysis degree can be increased.

The invention discloses a HD-Zip I type transcription factor GmHDL 57 gene with ability of improving salt resistance of leguminous plants. The sequence of the gene is a nucleotide sequence shown as SEQ ID NO:1, and a coded protein sequence is an amino acid sequence shown as SEQ ID NO: 2. The invention further discloses application of the HD-Zip I type transcription factor GmHDL 57 gene with ability of improving salt resistance of leguminous plants in lotus corniculatus. By utilizing an overexpression technique, the salt resistance of the gene in the leguminous plant lotus corniculatus is researched. Results show that compared with control plants, the salt resistance of the plants is obviously enhanced after overexpression, and the gene has great application prospects for widening a planting range of the leguminous plants in saline-alkali soil.

The invention discloses a production method of novel cattle and sheep animal husbandry composite biochemical forming forage grass, and relates to the field of feed processing. The composite biochemical forming forage grass comprises the following raw materials in parts by weight: 30%-60% of corn, 40%-65% of alfalfa, 12%-84% of caragana microphylla, 50%-90% of crop straw, 10%-40% of additional feed, 10%-30% of barley granules, 20%-50% of water-based sugar beet pulp, 8%-10% of cynodon dactylon, 10%-35% of wet brewer's grain, 8%-12% of white clover, 15%-35% of corncobs, 12%-25% of lotus corniculatus hay and 16%-50% of fish meal. The cattle and sheep animal husbandry composite biochemical forming forage grass processed by the scheme has a best feeding effect, cattle and sheep like to eat the forage grass, and the forage grass is rich in nutrition, the yield of cattle and sheep is greatly increased, and in addition, the production cost is smaller than 700 yuan / T, the feed grain is saved by 20%-40%, and the cost is reduced by 15% or above.

The invention provides a saline-alkali soil improvement method and application thereof. The improvement method comprises the following steps of step 1, fully mixing saline-alkali soil, a salt-dissolving growth-promoting bacterium agent, brown sugar and water, performing fermenting, and naturally air-drying to obtain salt-dissolving growth-promoting bacterium fermented soil; 2, uniformly mixing a humic acid aqueous solution with the fermented soil, naturally air-drying a mixture, and then removing salt and alkali separated out from the surface layer of the soil; and 3, repeating the step 2 formultiple times until no salt and alkali are separated out of the soil surface layer to finish improvement of the saline-alkali soil. According to the method for improving the saline-alkali soil through humic acid assisted salt-dissolving growth-promoting bacteria microorganisms, the saline-alkali content of the improved soil is reduced, the granular structure of the soil is moderate, the content of main organic matter is greatly increased, and salt-tolerant plants such as suaeda salsa, kalidium foliatum, lotus corniculatus and salicornia europaea can be directly planted in the improved saline-alkali soil; and the salt-tolerant plants can grow well, and the economic benefit of saline-alkali soil crop planting is improved.

The invention relates to feed for artificial bamboo rat breeding. The feed is prepared from 20-22 parts of bamboo shoots, 70-80 parts of corncob, 20-30 parts of soya bean meal, 8-10 parts of sesame seed meal, 25-28 parts of pennisetum hydridum, 3-8 parts of trifolium lupinaster, 5-7 parts of lotus corniculatus, 20-24 parts of watermeion peel, 25-30 parts of beetroot, 18-20 parts of purple cabbage, 0.01-0.012 part of tricalcium phosphate, 1.5-1.8 parts of fish oil, 0.3-0.6 part of vitamin E, 2-5 parts of brown granulated sugar and 0.8-1 part of glutamic acid. The feed is prepared according to daily energy and nutritional requirements of bamboo rats, is nutritionally balanced, rich in growth nutrient elements such as the vitamin, microelement and amino acid and capable of increasing the growth rate of the bamboo rats, makes furs of the bamboo rats bright and smooth, shortens the breeding cycle, reduces breeding cost and increases economic benefits.

The invention relates to the technical field of bioengineering and genetic engineering, in particular to an agrobacterium transformation vector based on a rhizobium type III effector NopP and application of the vector. The coding gene of the NopP is operably connected with a promoter in the vector to form an expression element, and the expression element is inserted into multiple cloning sites of a T-DNA sequence. The constructed agrobacterium transformation vector can further improve the hairy root transformation efficiency of Lotus japonicus, the probability of obtaining transgenic hairy roots is increased, the expression of NopP does not affect normal nodulation symbiosis of the Lotus japonicus and rhizobium, and the effectiveness and practicability of hairy root transformation of the Lotus japonicus are greatly improved.

The invention discloses a needle mushroom sticky rice juice beverage and a preparation method thereof. The needle mushroom sticky rice juice beverage comprises, by weight, 80-100 parts of needle mushrooms, 20-30 parts of birch juice, 60-80 parts of sticky rice juice, 20-30 parts of lotus root juice, 28-35 parts of strawberry wine, 16-18 parts of pigskin jelly, 1-2 parts of blueberry leaves, 1-2 parts of chufa, 2-3 parts of lotus corniculatus, 3-4 parts of hibiscus sabdariffa, 2-3 parts of longan leaves, 1-2 parts of gardenia florida, 6-8 parts of tender pistacia chinensis leaves, 8-12 parts of antichoke, 8-10 parts of tender moringa oleifera leaves, an appropriate amount of lemon vinegar, an appropriate amount of lotus leaves and 10-12 parts of nutrition additives. The needle mushroom sticky rice juice beverage is mellow, smooth and refreshing in taste, has the fragrance of the lotus leaves and is cool, refreshing and capable of restoring consciousness; various kinds of nutrient substances are added, and dietary fibers, collagen and other nutrient substances are used in proper proportions; various kinds of Chinese herbal medicinal ingredients are added and have an synergistic effect, so the needle mushroom sticky rice juice beverage has the effects of invigorating the stomach, promoting digestion, protecting the spleen, strengthening the liver, loosening the bowel to relieve constipation, engendering liquid, allaying thirst, maintaining beauty, keeping young and the like.

The present invention discloses a chicken endocrine balance maintaining health-care feed and a preparation method thereof. The health-care feed is prepared from the following raw materials in parts by weight: 500-600 parts of fresh cassava residues, 1.5-2.5 parts of sodium citrate, 74-76 parts of perilla seed meal, 56-58 parts of oatmeal, 4-5 parts of rhizoma alpiniae officinarum, 3-4 parts of red soy sauce, 34-36 parts of locust bean dregs, 45-47 parts of Chinese yam dregs, 23-26 parts of pine needle powder, 0.3-0.4 part of lactic acid bacterium fermentation agent, 35-37 parts of lotus corniculatus, 32-34 parts of corn gluten meal, 7-9 parts of tomato powder, 3-4 parts of carp meat, 0.2-0.4 part of yeast extract, 0.5-0.7 part of coix seed flour, 7-8 parts of plasma protein powder, 12-14 parts of brown seaweed powder, and 0.3-0.5 part of radix aucklandiae powder. The health-care feed improves the endocrine and material metabolism of chickens, increases the feed intake of the chickens, increases the intake of proteins, increases the egg production of the chickens, and improves the quality of chicken meat.

The invention relates to a pharmaceutical composition for treating bronchopneumonia. The pharmaceutical composition is composed of the following traditional Chinese medicine raw materials according to a weight ratio: 3-7 parts of winged laggera herb, 3-5 parts of old radish root, 2-5 parts of flower of aglaia odorata, 5-8 parts of whole herb of amethystea caerulea, 5-10 parts of whole herb of andrographis laxiflora, 3-7 parts of root of artemisia dracunculus, 3-7 parts of whole herb of lysimachia microcarpa, 2-8 parts of rhizome of mioga ginger, 3-5 parts of capitulum of picris hieracioides, 3-5 parts of whole herb of salvia substolonifera, 5-12 parts of involute spikemoss herb, 5-10 parts of cryptoporus volvatus, 3-8 parts of whole herb of juncellus serotinus, 3-7 parts of cudweed herb, 3-5 parts of ludwigia hyssopifo-lia (G.Don)Exekk, 2-7 parts of whole herb and flower of lotus corniculatus, 3-7 parts of roots and rhizomes of primula sieboldii, 5-15 parts of Indian rorippa herb and 5-15 parts of roots or whole herb of wahlenbergia marginata. The pharmaceutical composition has the efficacies of clearing heat, ventilating the lung, clearing away phlegm, relieving asthma, relieving cough, reducing sputum, invigorating qi and strengthening spleen, has a definite and remarkable curative effect for treating bronchopneumonia, is high in effective rate and safe, has no toxic or side effects and is low in possibility of relapse.

The invention discloses application of a lotus corniculatus seed extract and rhamnosyl uridine, and particularly relates to application of the lotus corniculatus seed extract and the rhamnosyl uridine in preparation of products for controlling oil and / or minimizing pores. It is verified for the first time that both the lotus corniculatus seed extract and the rhamnosyluridine separated from the lotus corniculatus seed extract have the effects of reducing skin grease secretion and shrinking pores, and the lotus corniculatus seed extract and the rhamnosyluridine can be widely applied to personal care products and have very good application prospects.

The invention discloses a medicine for treating asthma, belonging to the technical field of traditional Chinese medicines. The medicine is prepared from the following traditional Chinese herbal medicines: ludwigia hyssopifo-lia, lotus corniculatus , polygonum perfoliatum, common nepenthes, asaurm himalaicum, radix cynanchi bungei, dioscorea hemsleyi, cynanchum otophyllum, henry elaeagnus stems and leaves, rorippa globosa, serissa serissoides, white willow, ficus pumila, boehmeria silvestrii, dahlia pinnata, lily, bulbophyllum reptans, wild ginger, ludisia discolor, arundina graminifolia and geckos. The medicine disclosed by the invention has the effects of clearing away heat and toxic materials, invigorating spleen and removing damp, relieving cough and asthma, widening the chest to dispel melancholy as well as reinforcing qi and nourishing blood, acts appropriately on the root causes of diseases, is remarkable in curative effect and short in treatment course, and can treat both the symptoms and the root causes.

The invention discloses shampoo with oil control and acarus killing effects and a preparation method of the shampoo, and belongs to the technical field of cosmetics. The natural component composition is prepared from the following components: 1-6 parts of cortex dictamni, 2-14 parts of cacumen biotae, 3-15 parts of fructus cnidii, 1-10 parts of radix stemonae, 1-15 parts of raw rhizoma atractylodis, 2-15 parts of radix arnebiae seu lithospermi and 2-16 parts of lotus corniculatus, after the mixture is frozen and crushed into plant powder, an alkaline solution is added for soaking, ultrasonic treatment, enzymolysis, alcohol extraction and filtration are performed, residues are repeatedly extracted for 2-3 times, extracting solutions are combined, activated carbon adsorption and filtration are performed, and the natural component composition is obtained. And adding the plant extract into the shampoo. The shampoo has good oil control and acarus killing effects, and has a huge market promotion prospect.

The invention discloses a Lotis corniculatus L. stress resistance related transcription factor and a coding gene and application thereof. The invention also provides the structural domain of an ERF transcription factor shown as SEQ ID NO.3 capable of combining with a cis-acting element and starting expression of the stress resistance response gene. The functional analysis experiment proves that overexpression of the LcERF034 gene in plants can effectively increase or improve the resistance to adversity stress including high salt, drought and low temperature, therefore indicating that the protein encoded by the LcERF034 gene has the function of the ERF transcription factor. The invention further provides application of the transcription factor and the coding gene thereof to the enhancement of adversity stress resistance of plant or the cultivation of adversity stress tolerant transgenic new plant varieties.

The invention discloses germ-left rice fermented food and a preparation method thereof. The germ-left rice fermented food is prepared from the following components in parts by weight: 35-40 parts of germ-left rice, 15-20 parts of soybeans, 15-20 parts of corns, 5-8 parts of raisins, 1-2 parts of rosamultiflora, 1-3 parts of lotus flower distillate, 2-4 parts of lotus japonicas, 1-2 parts of tremella, 3-5 parts of radix glehniae, 1-3 parts of flos arecae, 1-2 parts of ginseng strip, 2-4 parts of rice bean leaves, 3-5 parts of radix codonopsis, 1-3 parts of chinese waxgourd peels, 3-5 parts of tea cream, 1-3 parts of mints, 2-4 parts of clove and 1-2 parts of salt. The germ-left rice fermented food is crisp in taste, strong in fragrance, easy to digest and absorb, and free of inflammation after being eaten.

The invention provides a traditional-Chinese-medicine medicament for treating nasosinusitis. The traditional-Chinese-medicine medicament is prepared from, by weight, 150-250 parts of pseudo-ginseng, 60-90 parts of vallisneria spiralis, 20-40 parts of lotus corniculatus, 20-50 parts of oriental stephania roots, 10-30 parts of goat-ear elecampane, 40-60 parts of calamus wailong roots, 60-80 parts ofcommon yam rhizomes, 3-9 parts of flos magnolia, 8-15 parts of gentiana scabra bge, 2-5 parts of radix angelicae, 2-5 parts of herba asari, 1-2 parts of divaricate saposhnikovia roots and 1-5 parts of cocklebur fruits. The traditional-Chinese-medicine medicament for treating nasosinusitis is short in medication time and good and stable in treatment effect, nasosinusitis does not relapse after drug withdrawal, and the traditional-Chinese-medicine medicament has the good treating effect on nasosinusitis particularly.

The invention discloses a leghemoglobin GmLbc2 gene involved in symbiotic nodulation of leguminous plants, which has a length of 438 bp and is located on chromosome 20 of soybean genome, and the invention also discloses an application of the gene in regulating the number of nodules of leguminous plants (Lotus corniculatus, Chinese milk vetch, clover, soybeans and peanuts). A reverse transcriptionPCR technology is used to isolate a soybean hemoglobin gene GmLbc2 in soybean root, and confirms that the gene is a leghemoglobin gene by homologous protein multi-sequence alignment and cladogram analysis; the application confirms for the first time that a soybean nodulation factor receptor protein GmNFR1a and the leghemoglobin GmLbc2 can interact in yeast and tobacco; further, a method of overexpression of genetic transformation confirms that the gene participates in and regulates the nodulation of legumes. The application confirms for the first time that the soybean leghemoglobin gene GmLbc2is regulating the symbiotic nodulation of legumes.

The invention relates to the technical field of biology, in particular to application of genes MsGCHI and MsADCS in increasing the folic acid content of plants and promoting plant growth. Under the greenhouse condition, the folic acid content and the precursor content in the leaves of the transgenic line are obviously higher than those in the wild type, the folic acid content in the leaves of the transgenic line is obviously higher than those in the stems, and the precursor content in the stems is higher than those in the leaves, so that the coexpression of the MsGCHI and the MsADCS can obviously improve the folic acid content and the precursor content in the lotus corniculatus. Meanwhile, the growth speed of the transgenic lotus corniculatus strain GA-3 is obviously superior to that of the wild type, and the branch number and root length of the transgenic lotus corniculatus strain GA-11 are obviously superior to those of the wild type, so that folic acid bioaugmentation has no adverse effect on the growth of the lotus corniculatus, and endogenous folic acid synthesized by folic acid bioaugmentation can promote the growth of the transgenic lotus corniculatus strain.

The invention discloses application of lotus corniculatus in the aspect of insect ecological regulation and control and an insect regulation and control method of the lotus corniculatus, and belongs to the technical field of pest prevention and control. On the one hand, application of the lotus corniculatus in insect ecological regulation and control is provided, and on the other hand, an insect regulation and control method based on lotus corniculatus is provided, namely, lotus corniculatus is planted in a target area where alfalfa thrips need to be regulated and controlled. The lotus corniculatus can obviously improve the density of orius minutus which is the natural enemy of thrips, a good shelter can be provided for the orius minutus, the thrip density is increased after the alfalfa area revives, and the thrip density can be controlled to not rebound to the high density before initial harvesting by means of the high-density natural enemy orius minutus preserved in the lotus corniculatus shelter, so that the aim of regulating and controlling thrip can be achieved.

The invention relates to the technical field of traditional Chinese medicine, in particular to traditional Chinese medicine eye drops for treating keratoconjunctivitis. The traditional Chinese medicine eye drops are prepared from, by weight, 10-15 parts of common scouring rush herb, 5-12 parts of fructus viticis, 4-8 parts of rhizoma atractylodis, 6-10 parts of radix polygoni multiflori, 5-8 parts of roots of hibiscus rosasinensis L., 5-10 parts of roots of lotus corniculatus L., 3-10 parts of cape jasmine, 12-15 parts of semen cassiae torae, 5-10 parts of cordyceps sobolifera, 15-20 parts of linear stonecrop herb, 10-13 parts of spica prunellae, 8-12 parts of radix angelicae pubescentis, 5-10 parts of bulbus lilii, 5-12 parts of honeysuckle flowers, 6-8 parts of flos chrysanthemi, 8-13 parts of climbing groundsel herb and 8-15 parts of radix ilicis asprellae. The traditional Chinese medicine eye drops for treating the keratoconjunctivitis has a very good treatment effect on conjunctivitis, has the effects of clearing heat and purging fire and is good in treatment effect, capable of quickly achieving the effects and free of toxic and side effects.

The invention relates to the field of genetic engineering and specifically relates to a trivalent HA-LTB fusion expression vector of a lotus corniculatus specifically-expressed H5N1 antigen protein. At a multiple cloning site of a lotus corniculatus plant expression vector, in a direction from a 2E+35 stator to a poly(A)-Nos terminator, an optimized HK97HA gene as shown in a nucleotide sequence SEQ ID No.2, an optimized HK07HA1 gene as shown in a nucleotide sequence SEQ ID No.4, an optimized SX06HA1 gene as shown in a nucleotide sequence SEQ ID No.6 and an optimized immunologic adjuvant LTB gene as shown in a nucleotide sequence SEQ ID No.8 are inserted in the forward direction in sequence. According to the invention, an established foreign protein high-level expression platform is utilized to express, so that a foundation is laid for developing broad-spectrum H5N1 bird flu plant edible vaccines.

The invention provides a fertilizer used for lycopersicon esculentum cultivation and a preparation method of the fertilizer. The fertilizer is prepared from the following raw materials, in parts by weight: 33-45 parts of decomposed cotton seed hulls, 20-30 parts of decomposed lotus corniculatus and 15-25 parts of plant ash. The preparation method comprises the following steps: weighing the decomposed cotton seed hulls, the decomposed lotus corniculatus and the plant ash according to the above ratio, performing uniform mixing and performing granulation to obtain the fertilizer. The fertilizer provided by the invention can improve soil and improve a yield and disease resistance of lycopersicon esculentum.

The invention discloses a drug for treating damp-heat type infantile abdominal distension. The drug comprises pratia nummularia, xylosma racemosum, aeschynomene indica, salvia substolonifera, abutilon roots, arundina graminifolia, mazus japonicas, cicada sloughs, lotus corniculatus, lotus plumule, shrubalthea bark, the bark of ash, excoecaria acerifclia, garden balsam seeds, mimosa pudica, Japanese clovers, bastard indigo, fallopia nervosa, adenosma indianum, premna microphylla, goodyera schlechtendaliana, lindernia ruellioides, notholirion hyacinthinum and fagopyrum tataricum. The drug for treating the damp-heat type infantile abdominal distension has the advantages that through scientific conditioning of bodies, good effect, quick action and less proneness to recurrence are achieved, and toxic and side effect is avoided.

The invention relates to an edible feed for newborn musk deers in suckling period and a preparation method thereof. The edible feed for the newborn musk deers in the suckling period comprises the following raw materials: nutritious forage, vitamin C, sodium alginate, mongolian dandelion herb, edible salt, and rapeseed oil; and the nutritious forage comprise the following ingredients: herba portulacae powder, diospyros lotus, sainfoin, crowtoe, honeysuckle, perilla frutescens, and Chinese sorghum stem. The edible feed is capable of providing nutrition for growth of the newborn musk deers in the suckling period.