The invention discloses application of a 
beauveria bassiana strain to prevent and control of pests on food crops and application to preparation of a 
pesticide for preventing and controlling the pests on the food crops. The food crops are paddy rice, beans, potatoes, highland barley, 
broad beans or wheat; the pests are 
lissorhoptrus oryzophilus, corn borers, thorn-sucking 
insect whiteflies, aphids, pine caterpillars, grubs, locusts, potato beetles, 
monochamus alternatus, termites, tea lesser leafhoppers and peach fruit borers; a culture method of the 
beauveria bassiana strain comprises the following steps: inoculating the strain on a culture medium for culturing at the temperature of 25 to 30 DEG C for 5 to 10 days, and then obtaining activated 
spore powder. The 
beauveria bassiana strain disclosed by the invention has the advantages of high 
spore-producing ability, good production character and high 
growth speed; in addition, the 
beauveria bassiana strain has the characteristics of high 
toxicity, high insecticidal speed and environment friendliness; by using an insecticidal fungal preparation produced by the 
beauveria bassiana strain, the number of the 
population of the pests such as the 
lissorhoptrus oryzophilus and the corn borers on the food crops can be effectively controlled.