189 results about "Peptidoglycan" patented technology

The present invention relates to three novel peptidoglycan recognition binding proteins expressed by keratinocytes, wound-healing tissues and chondrosarcoma tissue. More specifically, isolated nucleic acid molecules are provided encoding human peptidoglycan recognition protein-related proteins, referred to herein as PGRP-K (Keratinocytes), PGRP-W (Wound-healing), and PGRP-C (Chondrosarcoma) of FIGS. 1A–B, FIGS. 2A–C, and FIG. 3, respectively, each having homology to both human peptidoglycan recognition protein (PGRP) as well as murine Tag-7. PGRP-K, PGRP-W, and PGRP-C polypeptides are also provided. Further provided are vectors, host cells and recombinant methods for producing the same. The invention also relates to both the inhibition and enhancement of activities of PGRP-K, PGRP-W, and PGRP-c polypeptides and diagnostic methods for detecting PGRP-K, PGRP-W, and PGRP-C gene expression.

The present invention is a substantially purified sortase-transamidase enzyme from Gram-positive bacteria, such as Staphylococcus aureus. A specific sortase-transamidase enzyme disclosed has a molecular weight of about 29,076 daltons and catalyzes a reaction that covalently cross-links the carboxyl terminus of a protein having a sorting signal to the peptidoglycan of a Gram-positive bacterium, where the sorting signal has a a motif of NPQ / KTN / G therein. Variants of the enzyme, methods for cloning the gene encoding the enzyme and expressing the cloned gene, and methods of use of the enzyme, including for screening for antibiotics and for display of proteins or peptides on the surfaces of Gram-positive bacteria, are also disclosed.

The present invention is a substantially purified sortase-transamidase enzyme from Gram-positive bacteria, such as Staphylococcus aureus. The enzyme having a molecular weight of about 23,539 or about 29,076 daltons and catalyzing a reaction that covalently cross-links the carboxyl terminus of a protein having a sorting signal to the peptidoglycan of a Gram-positive bacterium, the sorting signal having: (1) a motif of LPX3X4G therein; (2) a substantially hydrophobic domain of at least 31 amino acids carboxyl to the motif; and (3) a charged tail region with at least two positively charged residues carboxyl to the substantially hydrophobic domain, at least one of the two positively charged residues being arginine, the two positively charged residues being located at residues 31-33 from the motif, wherein X3 is any of the twenty naturally-occurring L-amino acids and X4 is selected from the group consisting of alanine, serine, and threonine, and wherein sorting occurs by cleavage between the fourth and fifth residues of the LPX3X4G motif. Variants of the enzyme, methods for cloning the gene encoding the enzyme and expressing the cloned gene, and methods of use of the enzyme, including for screening for antibiotics and for display of proteins or peptides on the surfaces of Gram-positive bacteria, are also disclosed.

The invention relates to a marine-fish environmental protective compound feed, consisting of the following materials in weight ratio: 13 to 20 portions of domestic fish powder, 8 to 15 portions of biological fish meal ABP600, 8 to 12 portions of soybean meal, 10 to 18 portions of peanut bran, 3 to 10 portions of corn, 3 to 7 portions of cotton seed meal, 3 to 7 portions of rapeseeds meal, 3 to 5 portions of meat and bone meal, 3 to 5 portionsof squid bowels powder, 20 to 30 portions of high gluten flour, 1.0 to 3.0 portions of blood meal, 1.0 to 2.0 portions of soybean lecithin, 2.0 to 3.0 portions of marine-fish oil, 0.3 to 0.6 portions of choline chloride (50%), 0.05 to 0.1 portions of Vitamin C phosphate (35%), 0.15 to 1.5 portions of calcium biphosphate, 0.2 to 0.5 portions of compound multiplex vitamin, 0.5 to 1.5 portions of compound mineral salt, 0.1 to 0.3 portions of feed attractant, 0.1 to 0.3 portions of micro-bio-preparation, 0.03 to 0.08 portions of A3Alpha peptidoglycan, 0.01 to 0.04 portions of phytase, and 0.04 to 0.08 portions of non-starch polysaccharase. The compound foodstuff can meet the nutrition demands of such high density cultivated marine fishes such as weever, large yellow croaker, American red fish, sparidae fishes, and trachinotus ovatus and rudd etc, and the cultivated objects can rapidly grow and has high foodstuff conversion rate; the compound foodstuff can intensify the immunity and anti-stress ability of fishes and endow them with good vitality, vivid body color and high survival rate; compared with marine fished cultivated by other foodstuffs, the excretion rates of nitrogen and phosphorous of the marine fishes cultivated by the compound foodstuffs is lowered by 10 to 15 percent, and cause smaller contamination to the cultivation water body.

Co-administration of a lysostaphin or other anti-staphylococcal agent which cleaves cross-links of peptidoglycans of staphylococci cell walls such as lysostaphin and an antibiotic effective against staphylococci due to antibiotic activity mediated by cell-wall activity is effective against staphylococcal infection, even staphylococci that may be resistant to one or other of lysostaphin or the cell-wall active antibiotic. Co-administration simultaneously suppresses the generation of antibiotic-resistant mutant strains. Effective cell-wall active antibiotics include β-lactams and glycopeptides.

The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample.

Methods and compositions for detection of microbial contaminants in peritoneal dialysis solutions are provided. The methods and compositions employ modified bioburden testing and the detection of peptidoglycan. A novel cause of aseptic peritonitis is provided—aseptic peritonitis associated with gram positive microbial contamination of a dialysis solution. Peptidoglycan is a major component of a gram positive bacterial cell wall and thus can serve as a marker for gram positive bacteria. In this regard, testing for peptidoglycans can be utilized to effectively prevent peritonitis in patients that use the peritoneal dialysis solutions, such as peritoneal dialysis solutions that contain a glucose polymer including an icodextrin and the like.

Co-administration of a lysostaphin or other anti-staphylococcal agent which cleaves cross-links of peptidoglycans of staphylococci cell walls such as lysostaphin and an antibiotic effective against staphylococci due to antibiotic activity mediated by cell-wall activity is effective against staphylococcal infection, even staphylococci that may be resistant to one or other of lysostaphin or the cell-wall active antibiotic. Co-administration simultaneously suppresses the generation of antibiotic-resistant mutant strains. Effective cell-wall active antibiotics include β-lactams and glycopeptides.

The present invention relates to vaccines for treating bacterial infections, which vaccines comprise a glycoconjugate immunogen comprising at least one capsular polysaccharide conjugated to a carrier protein, such that the capsular polysaccharide contains an amount of peptidoglycan effective to improve the vaccine's properties.

The present invention is a substantially purified sortase-transamidase enzyme from Gram-positive bacteria, such as Staphylococcus aureus. The enzyme having a molecular weight of about 23,539 or about 29,076 daltons and catalyzing a reaction that covalently cross-links the carboxyl terminus of a protein having a sorting signal to the peptidoglycan of a Gram-positive bacterium, the sorting signal having: (1) a motif of LPX3X4G therein; (2) a substantially hydrophobic domain of at least 31 amino acids carboxyl to the motif; and (3) a charged tail region with at least two positively charged residues carboxyl to the substantially hydrophobic domain, at least one of the two positively charged residues being arginine, the two positively charged residues being located at residues 31-33 from the motif, wherein X3 is any of the twenty naturally-occurring L-amino acids and X4 is selected from the group consisting of alanine, serine, and threonine, and wherein sorting occurs by cleavage between the fourth and fifth residues of the LPX3X4G motif. Variants of the enzyme, methods for cloning the gene encoding the enzyme and expressing the cloned gene, and methods of use of the enzyme, including for screening for antibiotics and for display of proteins or peptides on the surfaces of Gram-positive bacteria, are also disclosed.

The invention relates to an invitro recombinant expression technology and functional identification of chlamys farreri peptidoglycan recognition protein (Cf-PGRP) gene. Cf-PGRP is provided with an amino acid sequence in a SEQ ID NO.1. The Cf-PGRP is obtained by the invitro recombinant expression technology, and the recombinant protein has broad sterilizing effect, obvious killing effect on Gram positive bacteria and the certain antibacterial effect on Gram negative bacteria and potential application value in aspects of developing broad antibacterial medicines, immune reinforcing agent, feed additive, and the like.

The invention relates to disease-resistant highly-effective expanded compound feed for trachinotus ovatus. The feed is made of raw materials in the following weight ratios: imported steam fish meal 13-40, imported straight fire fish meal 10-30, biological fish meal ABP600 5-8, soybean meal 5-10, fermented soybean meal 5-8, soya meal 2-4, cotton seed meal 3-5, rape seed meal 3-5, meat and bone meal 3-8, cuttlefish meal 2-5, strong flour 25-33, blood meal 1.0-3.0, soybean lecithin 1.0-3.0, harp seal oil 2.0-5.0, choline chloride (5 percent) 0.4-1.0, vitamin C-phosphate ester (35 percent) 0.1-0.3, calcium dihydrogen phosphate 0.5-1.5, compound vitamins 0.2-0.5, compound mineral salt 0.5-1.5, phagostimulant 0.1-0.3, micro-ecological preparation 0.1-0.3, Beta-1,3 glucan 0.1-0.3, A3Alpha peptidoglycan 0.03-0.08, phytase 0.01-0.04, and non-starch polysaccharase 0.04-0.08. Cultivation experiments prove that the compound feed can meet all the requirements on nutrition of trachinotus ovatus cultivation in mesh cages and ponds, the cultivated trachinotus ovatus grow rapidly, the feed conversion rate is high, the color of the feed is vivid, the feed coefficient is between 1.8-2.1, the cultivation benefit is remarkable, the vitality of the cultivated trachinotus ovatus is good, the survival rate thereof is 6.0-10.5 percent higher than the survival rate when feed of other brands is used, and the death rate during long-distance transportation is reduced by over 60 percent.

The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample.

The invention relates to a composite immunity intensifier for groupers, which comprises the following components according to weight percentage: 30 to 40 percent of Beta-dextran, 10 to 20 percent of A3 Alpha peptidoglycan, 10 to 20 percent of vitamin C-2- polyphosphate ester, 10 to 20 percent of levomisole, 5 to 10 percent of ginsenoside and 5 to 10 percent of vitamin E. The invention has the advantages that: firstly, the composite immunity intensifier effectively overcomes the problem that the body reverse feedback of the groupers is restrained and can be used in the whole culturing period of the groupers; secondly, the composite immunity intensifier as a feed additive is used in the compound feed of the groupers and only has 0.1 to 0.2 percent of addition amount; thirdly, the body immunity of the groupers is intensified obviously and the survival rate is improved; fourthly, the composite immunity intensifier has no bad effect on the palatability of feed; and fifthly, the raw material used by the composite immunity intensifier has stable source, low price and simple production process.

The present invention includes compositions, kits, and methods useful for the detection of bacteria. These agents and methods are primarily directed to a method of detecting the presence of bacteria in a sample, involving incubating the sample with an agent that binds to bacteria, such as, e.g., an agent specific for peptidoglycan or a component thereof, and then detecting bound bacteria. The invention includes lateral-flow immunoassay methods and devices for assessing the total bacterial load in a liquid sample.

The invention relates to an adsorbent for removing bacterial endotoxin, DNA and peptidoglycan. The adsorbent is connected with the surface of a carrier with good blood compatibility. The adsorbent is capable of effectively adsorbing bacterial endotoxin, DNA and peptidoglycan from blood of human body so as to achieve the aims of removing pathogene related molecular inducing the pyemia and exerting the effect of treating the pyemia, and the adsorbent has an important clinical value.

The present invention is a substantially purified sortase-transamidase enzyme from Gram-positive bacteria, such as Staphylococcus aureus. A specific sortase-transamidase enzyme disclosed has a molecular weight of about 29,076 daltons and catalyzes a reaction that covalently cross-links the carboxyl terminus of a protein having a sorting signal to the peptidoglycan of a Gram-positive bacterium, where the sorting signal has a a motif of NPQ / KTN / G therein. Variants of the enzyme, methods for cloning the gene encoding the enzyme and expressing the cloned gene, and methods of use of the enzyme, including for screening for antibiotics and for display of proteins or peptides on the surfaces of Gram-positive bacteria, are also disclosed.

Multi-drug resistant superbugs are a persistent problem in modern health care. This invention provides an antimicrobial endolysin-Lysostaphin triple fusion protein, comprising (1) an endolysin CHAP endopeptidase domain, (2) an endolysin amidase domain, and (3) a Lysostaphin glycyl-glycine endopeptidase domain. The domains are derived from two proteins that show antimicrobial synergy when used in combination. The protein has specificity and exolytic activity for the peptidoglycan cell wall of untreated, live Staphylococcus aureus from many growth phases i.e. stationary, logarithmic and biofilm growth. The recombinant triple fusion protein comprising the three functional antimicrobial domains is designed to be refractory to resistance development.

This invention pertains to the field of hyaluronic acid-binding synthetic peptidoglycans and methods of forming and using the same.

The invention discloses a synchronous production technology for preparing a dendrobium officinale oral liquid and a piece beverage, which is a method for extracting polysaccharide by exploding dendrobium plant cells combined an electronic program control (UHP) technology and ultrasonic water jet, and completely releases and dissolves peptidoglycan which is difficult to be extracted from dendrobium, and synchronously prepares an oral liquid or a beverage decoction piece. Low temperature, superhigh pressure and continuous flow cell disruption enables the plant cells to be instantly exploded and released, and under action of energy shearing effect, pressure intensity cavity effect and instant explosion effect, the plant cell is disrupted. Especially, dispersion, emulsification and particle nanocrystallization of the dendrobium officinale peptidoglycan substances maintain activity of the original substances, solves heat radiation difficulty, and avoids heat sensitivity substances to be destroyed. The technology is mainly applied to batch production of the high-content dendrobium peptidoglycan, and enables the dendrobium polysaccharide to be rapidly absorbed by a human body for prolonging life span.

The invention relates to a high-efficiency and environment-friendly puffed compound feed for snakehead fishes, which is prepared from the following raw materials in percentage by weight: 23-50 of imported steam fish meal, 5-15 of biological fish meal ABP600, 5-10 of soybean meal, 3-8 of cottonseed meal, 3-8 of rapeseed meal, 3-10 of meat and bone meal, 2-5 of squid visceral meal, 25-40 of high protein flour, 1.0-3.0 of blood meal, 1.0-3.0 of soybean lecithin, 2.0-5.0 of marine fish oil, 0.2-1.0 of choline chloride (50%), 0.05-0.3 of vitamin C-phosphate (35%), 0.3-1.5 of calcium biphosphate, 0.2-0.5 of complex multi-dimensional material, 0.5-1.5 of complex mineral salt, 0.1-0.3 of food attractant, 0.1-0.3 of microbial ecological agent, 0.03-0.08kg of A3a peptidoglycan, 0.01-0.04 of phytase and 0.04-0.08 of non-starch polysaccharide enzyme. The high-efficiency and environment-friendly puffed compound feed for snakehead fishes can meet complete nutrient requirements for high-density culture of snakehead fishes, enables the cultured fishes to grow quickly, has a feed coefficient between 0.9 and 1.2, and has the advantages of high feed conversion rate, obvious culture benefit, good energy of cultured fishes, high survival rate and long-distance transport resistance. Compared with the snakehead fishes cultured by other feeds, the snakehead fishes cultured by the feed of the invention have the advantages that the discharge rate of nitrogen and phosphorus can be reduced by 8%-15%, and the pollution to culture water is little.

The invention described herein provides molecularly imprinted polymers (MIPs) that are capable of binding to a microorganism, and methods for detecting and / or identifying microorganisms utilizing Molecularly Imprinted Polymers (MIPs). The microorganisms of the invention include prokaryotes, eukaryotes, virus and prions. The methods of the invention comprise detecting all or part, including epitopes, of macromolecules associated with the microorganisms. The macromolecules of the invention include polysaccharides, proteins, glycoproteins, peptidoglycans, lipoproteins, peptides, polypeptides, and polynucleotides, associated with said microorganisms. The invention also provides for methods of diagnosing a subject infected with the microorganisms utilizing MIPs, in addition to diagnostic kits.

The invention described herein provides molecularly imprinted polymers (MIPs) that are capable of binding to a microorganism, and methods for detecting and / or identifying microorganisms utilizing Molecularly Imprinted Polymers (MIPs). The microorganisms of the invention include prokaryotes, eukaryotes, virus and prions. The methods of the invention comprise detecting all or part, including epitopes, of macromolecules associated with the microorganisms. The macromolecules of the invention include polysaccharides, proteins, glycoproteins, peptidoglycans, lipoproteins, peptides, polypeptides, and polynucleotides, associated with said microorganisms. The invention also provides for methods of diagnosing a subject infected with the microorganisms utilizing MIPs, in addition to diagnostic kits.

The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample.

The invention discloses a special growth-promoting feed for a crab. The special growth-promoting feed comprises the following raw materials: extruded soybean, mashed potato, wheat flour, corn germ meal, wheat bran, pumpkin powder, jujube powder, Tenebrio molitor powder, shell powder, maggot powder, mysid powder, powder of internal organs of a squid, razor clam meat, Corcyra cephalonica eggs, kelp powder, spirulina powder, lard, Zhiqi fungal substance, Bacillus subtilis, Bacillus licheniformis, vitamin E, vitamin C, peptidoglycan, astragalus polysaccharide, fructo-oligosaccharides, lactic acid, glutamic acid, phosphoric acid, a traditional Chinese medicine additive, compound trace elements and composite nucleotide and protein peptide. The special growth-promoting feed provided by the invention is reasonable in formula and comprehensive in nutrition, and can meet the growth needs of the crab, promote growth of the crab, improve the immune system of the crab, and raise the survival rate of the crab.

Methods and compositions for detection of microbial contaminants in peritoneal dialysis solutions are provided. The methods and compositions employ modified bioburden testing and the detection of peptidoglycan. A novel cause of aseptic peritonitis is provided—aseptic peritonitis associated with gram positive microbial contamination of a dialysis solution. Peptidoglycan is a major component of a gram positive bacterial cell wall and thus can serve as a marker for gram positive bacteria. In this regard, testing for peptidoglycans can be utilized to effectively prevent peritonitis in patients that use the peritoneal dialysis solutions, such as peritoneal dialysis solutions that contain a glucose polymer including an icodextrin and the like.

The invention discloses a wastewater treatment agent for aquaculture. The wastewater treatment agent contains an ingredient A, an ingredient B, an ingredient C and an ingredient D, wherein the ingredient A is at least one of dicalcium phosphate, sodium dihydrogen phosphate, sodium selenite and magnesium sulfate; the ingredient B is at least one of DL-methionine, a L-lysine salt, tryptophane, threonine, betaine, astaxanthin, glucan, peptidoglycan, yeast and lipopolysaccharide; the ingredient C is at least one of sodium humate powder, sodium thiosulfate powder and aluminum potassium sulfate powder; and the ingredient D is at least one of glycyrrhiza uralensis, mung bean flour, acanthopanax bark, codonopsis pilosula, dried tangerine peel, Chuanxiong rhizome, gardenia, gypsum, dittany bark, folium isatidis, cortex phellodendri, radix isatidis, eupatorium fortunei and radix bupleuri. According to the wastewater treatment agent for aquaculture, through promoting the growth of plankton in culture water, the water treatment rate is accelerated, the self-purification ability of the water is improved, the improvement on water environment is promoted, influence on fishes in the water cannot be caused, and secondary pollution cannot be caused.

The present invention relates to a composition for selectively detecting an extremely small amount of peptidoglycan in sample, a preparation method of the composition, and a detection kit for peptidoglycan. It is possible to quantify a small amount of peptidoglycan contained in human blood, tissue, body fluid, water or food, and to diagnose an infection of microorganism with peptidoglycan as a component of cell wall using the composition and the detection kit. In addition, the composition can be applied for a diagnosis reagent of detecting an infection of Gram-positive bacteria in animal or human being in advance, and thus, can be used for the prevention or treatment of food poisonings and bacterial sepsis.