The invention relates to a method for culturing gamma-
delta-T cells, and more specifically relates to a method for in-vitro amplification of gamma-
delta-T cells. The method comprises the following operating steps: pre-
coating a T75 culture 
bottle with a TCR-gamma-
delta resisting 
antibody and CD28McAb for later use; isolating 
peripheral blood mononuclear cells (PBMC) of patients; regulating PBMC concentration to 1*10<6>  / ml with a serum-free culture medium which contains 5% of 
autologous plasma, and transferring the PBMC 
cell suspension into the T75 culture 
bottle; adding an initial culture medium which contains proper concentrations of Zoledronat, HSP70, 
Toll-like Receptors7 (TLR7) ligand, 
Levamisole (LMS), IL-2, IL-7 and IL-15; culturing in a saturated humid environment containing 5% of CO2 at 37 DEG C; according to growth situation of the 
cell, changing the culture medium every 2 to 3 days so as to control the 
cell concentration at about 2.5*10<6> / ml; meanwhile, compensating full doses of Zoledronat, HSP70, 
Toll-like Receptors7 (TLR7) ligand, 
Levamisole (LMS), IL-2, IL-7 and IL-15;; and continuously culturing for 12to 16 days so as to obtain a great amount of gamma-delta-T cells which are comparatively high in purity.