The invention discloses a tobacco active biological organic-inorganic special 
fertilizer and a preparation method thereof. The tobacco active biological organic-inorganic special 
fertilizer is prepared by the following steps of: selecting functional microbial strain and adding the following materials in percentage by weight: 50-70 percent of 
rapeseed meal, 20-40 percent of 
sesame seed meal and 10-20 percent of bean 
meal; mixing, then performing 
solid fermentation and performing enzymolysis on crude 
protein in cake meal to form a biological 
fermentation cake 
fertilizer with bioactive 
free amino acid and active 
small peptide; and then uniformly mixing with an activator and an 
inorganic fertilizer and chelating to prepare the tobacco active biological organic-inorganic special fertilizer. The tobacco active biological organic-inorganic special fertilizer is characterized by comprising the following raw materials in percentage by weight: 8-12 percent of activator, 20-30 percent of biological 
fermentation cake fertilizer and 55-75 percent of 
inorganic fertilizer. According to the invention, a method of combining an 
organic fertilizer with an 
inorganic fertilizer and combining 
nitrate nitrogen with 
ammonium nitrogen is adopted, the biological fermentation cake fertilizer with the 
rapeseed meal, 
sesame seed meal and bean meal as carriers is added, and the raw materials are mixed with the activator and the like to prepare a high-efficiency active fertilizer. The fertilizer has the characteristics of high 
nutrition, 
high activity and high efficiency; the tobacco 
plant root environment can be improved, the 
root system activity is enhanced, the leaf growth is promoted, the requirements of tobacco 
plant development on mineral 
nutrition are fully met, the yield of the tobacco 
plant and the quality of the 
tobacco leaf can be obviously improved, the environment 
pollution is reduced, and the soil is improved.