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117 results about "Rat tail" patented technology
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Method for constructing three-dimensional neural stem cell model in two steps by adopting micro-fluidic technology
ActiveCN103146650AReduce the cost of trainingReduce usageNervous system cellsTissue/virus culture apparatusCollagen iNeurogenesis
The invention relates to a method for constructing a three-dimensional neural stem cell model in two steps by adopting the micro-fluidic technology. The method is characterized in that a rat tail collagen I is used as a three-dimensional support, a micro-pillar array type micro-fluidic chip is used as a culture platform, and a neural stem cell is cultured in two steps, wherein in the early culture stage, a culture medium for promoting the amplification of the neural stem cell is injected into a cell culture chamber, and in the later culture stage, a conditioned medium suitable for the growth of the neural stem cell and the daughter cells thereof is used, and a three-dimensional composite structure which is similar to a nerve tissue is formed by simulating the microenvironment of different neurogenesis stages in the body. The method provided by the invention is good in repeatability and can be used for construction a plurality of groups of samples. The adopted microfluidic culture system is in a microliter volume and can be regulated accurately, thus the amount of various high-cost cell growth factors, immunologic fluorescent antibodies and cell hormones used in the process of culturing the cell can be reduced greatly, and the cell culture cost can be lowered. The three-dimensional neural stem cell model is expected to be a nerve tissue substitute for screening a novel medicament or monitoring an environmental toxin.
Owner:DALIAN UNIV OF TECH
Method for inducing bionic calcification in collagen fibers through polymer polyelectrolyte, and applications thereof
ActiveCN107224609AObvious superiorityHigh mechanical strengthPharmaceutical delivery mechanismTissue regenerationFiberCationic polyelectrolytes
The present invention relates to a method for inducing the bionic calcification in collagen fibers through a polymer polyelectrolyte, and applications thereof. The method comprises: treating different types of collagen scaffolds in a calcium phosphate mineralized solution containing a cationic polyelectrolyte or anionic polyelectrolyte to obtain the bionic calcification collagen scaffold material having the inside-fiber mineralization characteristic, wherein the collagen scaffold is recombinant collagens type I having different sources, completely demineralized bone collagen, completely demineralized dentin collagen, or rat tail collagen, and the mineralization liquid is a calcium phosphate solution added with a cationic polyelectrolyte or anionic polyelectrolyte. According to the present invention, through the stabilizing effect of the polyelectrolyte on the calcium phosphate solution, the novel bionic calcified collagen scaffold material with characteristics of similar natural bone tissue structure, good mechanical property, good biocompatibility and suitable biodegradability is constructed.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Method for culturing bladder cancer organs in vitro
ActiveCN111197030AImprove the success rate of cultivationAvoid conditions prone to hypoxiaCell dissociation methodsCell culture supports/coatingRat tailPharmaceutical drug
The invention discloses a method for culturing bladder cancer organs in vitro. The method comprises the following steps: manufacturing a gas-liquid interaction culture system, namely manufacturing a uniformly paved rat tail collagen supporting layer on the surface of a porous culture membrane in a Transwell upper chamber; re-suspending fresh in-vitro bladder cancer tissues by using a rat tail collagen solution, uniformly mixing, adding the obtained mixture onto the rat tail collagen supporting layer, and then putting the rat tail collagen supporting layer into an incubator at 37 DEG C to solidify so as to obtain a rat tail collagen layer containing the bladder cancer tissues; adding an organoid culture medium into a Transwell lower chamber, and enabling the liquid level of the culture medium to be lower than the rat tail collagen layer containing bladder cancer tissues; and carrying out passage and cryopreservation. The culture success rate of the bladder cancer organoid is remarkablyincreased, the bladder cancer organoid with reserved immune cells can be obtained through culture, operation is easy, the utilization rate of tumor tissue is high, and great significance and value areachieved for bladder cancer drug screening research.
Owner:上海嗣新医药科技有限公司
Experimental visual rat tail vein fixator
The invention discloses an experimental visual rat tail vein fixator comprising a base, wherein the base is provided with a fixed block and a regulating rod; one end of the regulating rod is movably connected with the fixed block or base, and the other end of the regulating rod is provided with a tail supporting frame; the distance between the tail supporting frame and the fixed block can be regulated through regulating the regulating rod; the top of the tail supporting frame is provided with a chute; a horizontally-irradiating yellow fluorescent lamp is embedded in the side wall of the chute; the fixed block is provided with a first fixed groove; the fixed block is provided with a pressing block which can be opened or closed relative to the fixed block so as to press or release a rat tail. The experimental visual rat tail vein fixator also has the advantages of simple and compact structure, low manufacturing cost as well as simplicity and convenience in use.
Owner:李晓峰
Cell electrochemical sensor for analyzing joint toxicity of fungaltoxin
The invention discloses a cell electrochemical sensor for analyzing joint toxicity of fungaltoxin, belonging to the fields of analysis and detection of food quality. According to the cell electrochemical sensor, the sensitivity of the sensor is increased by virtue of electroplating gold nanoparticles, an in-vivo physiological living environment of cells is simulated by virtue of laminin, and a three-dimensional model is constructed by virtue of rat tail collagens so as to relatively truly reappear cell morphology; and the constructed cell electrochemical sensor can rapidly and sensitively respond to the influences caused by the fungaltoxin to human hepatoma carcinoma cells, equipment is convenient and rapid and low in production cost, and by combining with an electrochemical resistance value and a joint index method, the type and degree of a joint action of the fungaltoxin can be rapidly judged. The constructed portable cell electrochemical sensor is successfully applied to the toxicity analysis of the fungaltoxin in foods and has good application prospects in the analysis of the joint toxicity of the fungaltoxin.
Owner:JIANGNAN UNIV
Technique for producing sargassum thunbeergii kuntze offspring
The invention discloses a seedling producing technique for rat-tailed algae, applying to seedling breed in-door according to sexual reproduction law, including: selecting and collecting the seed algae, stimulating and diffusing the seed algae, collecting the juvenile sporophyte, culturing and catadromousing seedlings, provided an artificial seedling producing technique for rat-tailed algae, dissolving the current shortcoming problem of the rat-tailed algae seedlings.
Owner:YANTAI UNIV
Active collagen lyophilized powder and preparation method thereof
ActiveCN111671667AImprove stabilityKeep natural activityCosmetic preparationsToilet preparationsPolyolFreeze-drying
The present invention discloses an active collagen lyophilized powder and a preparation method thereof. A rat tail collagen, polyols, mycose and citric acid are compounded, so that the stability of the rat tail collagen in a freeze-drying process may be jointly improved, a prepared lyophilized product may be stored without a preservative for a long time and is good in redissolution property, capable of keeping the natural bioactivity of collagen and remarkable in capability of promoting the synthesis of collagen in the skin.
Owner:GUANGDONG ZHONGERJIAN BIOTECH CO LTD
Method for separating and cultivating rat hepatocytes
InactiveCN101538552AExcellent adhesionA large amountTissue cultureBovine serum albuminCell separation
The invention provides a method for separating and cultivating rat hepatocytes. A rat is anaesthetized by Nembutal and is given sodium heparin for anticoagulation; portal catheterization is carried out; two-step reperfusion is carried out for perfusion; a digestive mature liver is taken off; hepatocytes are collected in Hanks liquid containing 1 percent of bovine serum albumin (BSA); hepatocyte suspension is screened by a screen stencil; after the filtrate is centrifugated and deposited, the deposit is collected in Leibovitz's-15(l-15) complete medium; the density of hepatocyte is adjusted to 3 to 6 *10<5> / ml and inoculated on a culture plate coated with rat tail collagen in the environment of temperature of 37 DEG C and 5 percent of CO2; and unattached and dead hepatocytes are removed by changing medium after 4 hours of inoculation, and the hepatocytes can be used for experiment after 20 hours of cultivation. The method is easy, simple and stable, has low cost and high efficiency, can obtain high-activity hepatocytes, is easy to attach, and can be used for pharmacological and toxicity experiments and popularized in common laboratories.
Owner:YANGZHOU UNIV
Composite collagen gel
InactiveCN1927393APromote absorptionExtended release timeOrganic active ingredientsPeptide/protein ingredientsDiseaseRelease time
The invention relates to a kind of compound collagen gel, which is the mixture of collagen and antibiotics. For every gram gel, there is 1-100mg collagen, which is extracted from tendon of the rat tails, swine, or ox, 1-100mg antibiotics, 0.1-1.0mg medical antiseptics, 10-500mg thickening agents, and water. The antibiotics and collagen act cooperatively to be used in the treatment of the following diseases: different kinds of open wound, anabrosis caused by diabetes, skin damage because of venereal disease, hyperkeratesis, cuticularized skin problems such as keratosis palmaris ET plantaris, winter cutitis and ichthyosis. Since the collagen protein in the gel can be easily absorbed to provide threpsis to the diseased region, so it can promote the agglutination very effectively as well as extend the time of releasing time of the antibiotics.
Owner:佟刚
Endothelial progenitor cell (EPC) culture method
InactiveCN104388388AReduce the cost of trainingThe cultivation method is simple and feasibleBlood/immune system cellsTube formationPeripheral blood mononuclear cell
The invention discloses an endothelial progenitor cell (EPC) culture method. The culture method comprises the following steps: inoculating human peripheral blood mononuclear cells in a cell culture bottle coated by I-type rat tail collagen, adding a DMEM culture medium in the cell culture bottle, putting the cell culture bottle in an incubator with the temperature of 37 DEG C, the CO2 content of 5 percent and the humidity of 95 percent for culturing; carrying out EPC digestion passage; then carrying out flow cytometric analysis on EPC; carrying out EPC immunofluorescence assay; carrying out a cell proliferative assay; carrying out a single cell clonogenic assay; carrying out FITC-UEA-1 extracellular adsorption and endocytosis Dil-acLDL experimental measurement; and carrying out an in-vitro tube formation experiment. The EPC culture method is simple, convenient and feasible. With the adoption of the EPC culture method, the cost can also be greatly lowered; the high-purity I-type rat tail collagen is prepared by the EPC culture method and used for culturing EPC by coating the cell culture bottle, and the culture medium which is low in cost is also adopted, so that the EPC is successfully cultured from human peripheral blood; through identification, the EPC cultured by the method disclosed by the invention has the phenotypic characteristics and the functions of the EPC cultured by the conventional method.
Owner:贾瑞鹏
In vitro tumor cell 3D collective invasion model and construction method thereof
ActiveCN108795872AGuaranteed persistenceGuaranteed purityGenetically modified cellsCell culture supports/coatingRat tailTumor stem cell
The invention provides an in vitro tumor cell 3D collective invasion model and a construction method thereof. Tumor multicellular organ groups are prepared; cells contain light conversion fluorescentprotein which is coated into type I rat-tail collagen for carrying out 3D culture and the growth state, a proliferation mode and an invasion mode of tumor cells are simulated in vitro. The model studies the collective invasion effect of tumor multicellular spherical organs and can simulate the collective invasion mode of the tumor cells in vivo; after photobleaching is carried out by using a laserconfocal microscope, Leader Cells and Follower Cells in the collective invasion cells can be distinguished under the condition of lacking specific biological markers; in addition, the tumor cells ina target region can be obtained by fluorescence flow separation for carrying out follow-up experimental study.
Owner:SOUTHERN MEDICAL UNIVERSITY
Low temperature preservation method of sperm pods of Litopenaeus vannamei
The invention discloses a method for storing spermatophore of litopenaeus vannamei at low temperature. The method comprises the following steps of: acquiring the spermatophore, fixing the spermatophore, storing the spermatophore and dyeing spermatozoa. In the method, a culture layer for the spermatophore of the litopenaeus vannamei is prepared from 0.3 to 0.4 part of 1.8 to 3.0 percent self-prepared type I liquid rat tail collagen serving as a medium for contracting the culture layer, 0.3 to 0.4 part of culture solution of 2*1,640, 0.1 to 0.2 part of fetal calf serum in an American GIBCO company and 0.1 part of Matrigel (a soluble basilar membrane matrix) in an American Sigma company, wherein the culture layer is viscous at normal temperature, and can be solidified at the temperature of 40 DEG C and can wrap the spermatophore. The spermatophore is packaged in separate an epoxy resin (EP) tube at the normal temperature, the spermatophore is wrapped by the prepared culture layer, and the EP tube wrapped with the spermatophore is arranged in a refrigerator to be stored at the low temperature of 4 DEG C. The method has the characteristics of simplicity of operation, complete storage of a spermatophore structure, high survival rate of the spermatozoa, long retention time, stable effect of low-temperature storage and the like and is easy to popularize and apply. The retention time of the spermatophore is at least 3 months, and the survival rate of the spermatozoa is over 80 percent.
Owner:GUANGXI INST OF FISHERIES
Non-invasive rat tail blood pressure measuring device
ActiveCN107212871AFree height adjustmentEasy to measureEvaluation of blood vesselsAnimal fetteringTailRat tail
The invention discloses a non-invasive rat tail blood pressure measuring device which comprises an experiment tray and also comprises a rat body fixing component and a pulse detecting component. The rat body fixing component and the pulse detecting component are arranged on the experiment tray, the rat body fixing component is used for limiting the body of a rat and keeping the rat calm, and the pulse detecting component is used for measuring rat tail blood pressure. The device can effectively limit the bodies and tails of different sizes of rats, especially small sizes of rats, keep the rats calm and facilitate rat tail blood pressure measurement, and the measurement accuracy is effectively improved.
Owner:CHENGDU TME SOFTWARE
Preparation method of rat tail collagen protein
InactiveCN102586372AConnective tissue peptidesPeptide preparation methodsDry weightDrug biological activity
The invention relates to a preparation method of a rat tail collagen protein. A novel extraction scheme for mixing an enzymic method and an acid method is adopted, and a method for salting out and centrifuging by using solutions with different salinities for many times is adopted, so that the purification step is greatly simplified. By using the preparation method used by the invention, collagen proteins with the dry weight of 200mg can be extracted from each rat tail, the dry weight of the collagen proteins is increased by about 20 times than 10mg of collagen proteins extracted from each rat by using the traditional acid-leaching method, and defects and problems such as low extraction ratio, difficulty in purification, damage to the biological activity of the collagen protein and the like in the traditional method are avoided. Appraised by SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophresis), the obtained rat tail collagen protein obtains stripes with three clauses, and compared with the traditional commercial rat tail collagen protein of the SIMGA company, the rat tail collagen protein provided by the invention has no difference for atlases. Through analyzing the content of the amino acid, the prepared rat tail collagen protein is high in quality and purity.
Owner:广东省医学实验动物中心
Culture method for eriones unguiculatus primary liver cells
InactiveCN102952776AExcellent adhesionPreserve cell functionArtificial cell constructsVertebrate cellsCulture cellCentrifugation
The present invention discloses a culture method for eriones unguiculatus primary liver cells. The method comprises: (1) collecting a liver cell primary suspension from digested and matured isolated eriones unguiculatus liver, filtering, removing impurities from a filtrate, adding 5% calf serum-containing HBSS, repeatedly carrying out centrifugation washing, collecting cell precipitate and adding to a 20% new born calf serum-containing DMEM complete culture medium, and adjusting a liver cell concentration to 3.0*10<5>-5.0*10<5> / mL to prepare a liver cell suspension; and (2) inoculating the liver cell suspension in a culture plate coated with rat tail collagen, culturing at a temperature of 37 DEG C in a 5% CO2 environment, removing impurities after 4 hours, changing the culture medium into a maintenance culture medium containing cell factors, and carrying out cell adherence overnight to obtain the eriones unguiculatus primary liver cells. The method has characteristics of low cost, economy, practicality, and simple and easy-performing operation, wherein the obtained liver cells have characteristics of strong adherence, high vitality, high survival rate, stability and reliability, and the method is suitable for mass production.
Owner:ZHEJIANG ACAD OF MEDICAL SCI
Device and method for patterning co-culture of multiple cells
ActiveCN106479893APatterned realizationPromote growthArtificial cell constructsSkeletal/connective tissue cellsDimethyl siloxanePhotolithography
The invention provides a device and method for patterning co-culture of multiple cells. Adhesive tape is punched through a punching needle to form an array with micrometer-order holes, the array is used as a template, polydimethylsiloxane is directly poured to the template to obtain a channel with micrometer-order pillar array inside, polydimethylsiloxane is printed on a cell petri dish or culture plate base, the surface of the cell petri dish or culture plate base is not processed, or a rat tail collagen layer is incubated on the surface to form the culture device. The method of cell culture through the device is provided, the device can be manufactured just by the adhesive tape, the punching needle, a nicking tool and polydimethylsiloxane without photolithography, the device can be used for patterning any kind of cells, co-culture of the multiple cells is achieved, and limitation to photolithography by traditional micro-fluidic chip preparation is broken through.
Owner:UNIV OF SCI & TECH BEIJING
Application of rat tail collagen in field of cosmetics
InactiveCN109431850AImmutableIncrease contentCosmetic preparationsToilet preparationsSkin repairCollagen activity
The invention belongs to the field of cosmetics research and development, and particularly relates to application of rat tail collagen in the field of cosmetics. Compared with conventional collagen onthe market, in addition to the characteristics of general collagen, the rat tail collagen has the advantages of stable triple helix structure, high collagen activity and low immunogenicity; the source quality is controllable, the biosafety is high, and the reduction of biological activity by radiation sterilization is not needed; through experimental determination, the rat tail collagen preparedaccording to the technical scheme has the performance of whitening, melanin synthesis inhibition, anti-oxidation, anti-allergy, hormone skin repair and acne removal. The application of the rat tail collagen in the field of cosmetics overcomes the technical defect that the quality of the cosmetics is seriously affected due to addition of toners, flavoring agents, stabilizers and preservatives intocollagen-containing cosmetics in the prior art.
Owner:GUANGDONG ZHONGERJIAN BIOTECH CO LTD
Method for separating and culturing macaque adult hepatic precursor cells
InactiveCN101864394AEfficient separationSimple methodVertebrate cellsArtificial cell constructsDiseaseCultured cell
The invention relates to a method for separating and culturing macaque adult hepatic precursor cells, in particular to a separation and culture solution of the macaque adult hepatic precursor cells, belonging to a separation and culture solution of animal precursor cells. The method comprises the following steps: digesting a small quantity of hepatic tissues obtained by a macaque liver removal surgery or other ways by utilizing IV-type collagenase; collecting and suspending the cells in 10% of a fetal calf serum DMEM for culture; selecting and then inoculating a single cell block in the culture plate of a rat tail collagen bed board, wherein the culture solution is the special one; and finally sorting E-cad+cells from the cultured cells by the flow cytometry to obtain the target cells. The cells separated by the method of the invention can be applied to cell differentiation mechanism study and can become ideal master cells for treating late-period hepatic diseases.
Owner:昆明亚灵生物科技有限公司
Cytoskeleton for tissue-engineered tympanic membrane
The invention discloses a membranous matrix (cytoskeleton) which is prepared by mixing collagen and chitosan and then drying the mixture. The preparation process is simple. The collagen component is collagen I extracted from the rat tails, accounting for 90% of the weight of the mixture. The chitosan component is 75-95% of deacetylated chitosan, accounting for 10% of the weight of the mixture. The invention adopts the composite membrane as the cytoskeleton for constructing the tissue-engineered tympanic membrane and finds that the composite membrane is suitable for grow and proliferation of epithelial cell / keratinocyte and fibroblast of the tympanic membrane and can maintain good collagen secretion function of the fibroblast. The cytoskeleton further lays the foundation for constructing the tissue-engineered tympanic membrane.
Owner:孙建军 +1
Method of promoting differentiation of rat neural stem cells
The invention discloses a method of promoting differentiation of rat neural stem cells, belonging to culture of undifferentiated cells. The method comprises shredding brain tissues of fetal rats in 13-day pregnant SD rats, digesting into unicells, centrifuging, suspending the cells with a neural stem cell complete culture medium, sieving, culturing to obtain high-purity brain neural stem cells; collecting and centrifuging neural stem cells of a second generation, inoculating the cells with a neuron complete medium together with a 5 % fetal calf serum onto a 24-well plate already coated with rat tail collagen, adhering the cells onto the wall, starting to use the neuron complete medium at the first day of cell differentiation, simultaneously adding 10 [mu]l of 100 * 100 [mu]mol / L taurine into each hole, and culturing for 12 days to obtain the neural stem cells with differentiated neurons. Beneficial effects of the invention are that components of the nutrient solution are simple, cost is low, the added taurine exists in the body and thus usage is safe, and an amount of the generated mature neurons can be increased.
Owner:天津市环湖医院
Changeable square fixing device
The invention relates to a changeable square fixing device. The fixing device is provided with a base, a movable baffle, a fixed cylindrical body and a movable cylindrical body. The fixed cylindrical body and the movable cylindrical body are located on the base, and an arc-shaped cavity is formed between the fixed cylindrical body and the movable cylindrical body. The movable cylindrical body is provided with a radial sliding rail, and the sliding rail is matched with a radial sliding block on the base and can accordingly move on the base in the radial direction. One end of the fixed cylindrical body is provided with a tapered limiting plate. The movable baffle is provided with a fixing inserting plate and a stepped supporting plate. A U-shaped groove and a movable guide rail are arranged at the upper end and the lower end of the stepped supporting plate respectively. The movable baffle can axially move on the base through match of the movable guide rail and an axial sliding block of the base. The fixing inserting plate is provided with a rat tail groove and is embedded in the arc-shaped cavity between the fixed cylindrical body and the movable cylindrical body. The changeable square fixing device has the advantages that different sizes of rats can be fixed by adjusting the fixed space, the fixed rat body can be exposed outside, experiment research is facilitated, and the fixing device is simple in structure and reliable in fixing.
Owner:YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
Rat caudal vertebra intercalated disc minimally invasive puncture injection device
InactiveCN104622598AAchieve minimally invasive punctureImprove stabilityVeterinary instrumentsCylindromaRat tail
The invention relates to a rat caudal vertebra intercalated disc minimally invasive puncture injection device which is used for conducting minimally invasive puncture or injecting liquid on a fibrous ring in a rat tail. The rat caudal vertebra intercalated disc minimally invasive puncture injection device comprises a lantern ring, a needle handle and a needle plug. The lantern ring is of a hollow cylinder structure, the middle of the lantern ring is a sleeving hole used for sleeving the rat tail, a needle handle insertion hole and a locating hole are formed in a lantern ring body, and the needle handle insertion hole and the locating hole are arranged in a spaced mode and both communicated with the sleeving hole; the needle handle comprises a clamping portion of the upper end, a middle insertion portion and a needle, the insertion portion can be inserted and connected in the needle handle insertion hole, and a needle plug hole is formed in the clamping portion; the needle plug can be inserted into the needle plug hole to block the needle handle. Compared with the prior art, according to the rat caudal vertebra intercalated disc minimally invasive puncture injection device, the lantern ring is fixed on the rat tail, and the needle handle is inserted into the lantern ring, so that the minimally invasive puncture to the rat tail is achieved; the structure is simple, and the operation is convenient; meanwhile, the stability to the rat tail is stronger, the lantern ring can rotate on the rat tail, and therefore a circle of minimally invasive puncture on the fibrous ring can be conducted.
Owner:TONGJI UNIV
Absorbable haemostatic material prepared based on rat tail collagen and preparation method of same
InactiveCN102526796AIncrease productivityAvoid rising costsPeptide/protein ingredientsAerosol deliveryPolyvinyl alcoholFreeze-drying
The invention relates to an absorbable haemostatic material prepared based on rat tail collagen and a preparation method of the absorbable haemostatic material. The absorbable haemostatic material is prepared from the rat tail collagen, polyvinyl alcohol and chitosan with respective weight percentages of 1%-10%, 0.2%-5% and 0.2%-2%, and the balance of water by a freeze-drying technology. The haemostatic material of the invention is mainly prepared with the rat tail collagen; compared with disadvantages that the conventional haemostatic material is unabsorbable, body allergy is easily caused, the haemostatic effect is poor, and the like, the haemostatic material prepared by the invention is absorbable in a human body, good in biocompatibility, quick in hemostasis and strong in hydrophilia; platelet aggregation can be activated to form a thrombus, thereby promoting the plasma agglomeration and stopping blood flowing; and a specific region on the surface of the collagen can be bound with glycoprotein similar to fibronectin on the surface of a cell, so that the haemostatic material has a function of preventing intestinal adhesion.
Owner:广东省医学实验动物中心
Experimental rat tail-cutting blood collection device
ActiveCN106264773APrevent tamperingAchieve fixationAnimal fetteringSurgical veterinaryBlood collectionRat tail
The invention discloses an experimental rat tail-cutting blood collection device in the field of experimental apparatuses. The experimental apparatus comprises a front chamber and a rear chamber in sequence. The front chamber and the rear chamber are fixedly connected to each other. The front chamber comprises a trapezoidal plate and a conical plate. Two bevel edges of the trapezoidal plate are fixedly connected to a bottom edge of the conical plate, respectively. The rear chamber comprises a rectangular plate and an arch plate. Two long edges of the rectangular plate are fixedly connected to a bottom edge of the arch plate respectively. A plurality of air vents are circumferentially formed in the conical plate and the arch plate in uniform distribution. One end of the arch plate is hinged to a door sheet matching with the arch plate, and a concentric through hole is formed in the door sheet. A locking groove is formed in the edge of the door sheet, and a connecting buckle fastened to the locking groove is disposed on the rectangular plate. By using the experimental rat tail-cutting blood collection device, as an experimental rat lies in the device, the four limbs of the experimental rat cannot straighten; thus, the experimental rat can be prevented from moving in the front chamber and the rear chamber, fixation of the experimental rat is achieved, and the problem of unsuccessful blood collection caused by the fact that the cut tail enters a plastic bottle due to restless movement of the experimental rat is avoided.
Owner:ZUNYI MEDICAL UNIVERSITY
In vitro culture method of cervical intraepithelial neoplasia cells
InactiveCN103602630AImprove survival rateHigh purityVertebrate cellsArtificial cell constructsHuman papillomavirusCulture fluid
The invention discloses an in vitro culture method of cervical intraepithelial neoplasia cells. The in vitro culture method comprises the following steps: step one, taking a small piece of human cervical neoplasia tissue; step two, after digesting and decomposing by a type I collagenase, preparing a cell suspension; step three, utilizing a trypan blue dye exclusion method to judge the cell activity; step four, introducing the cell suspension into a culture bottle containing a low fetal bovine serum culture medium and coated with rat tail collagen, and waiting for the cells to adhere to the wall; and step five, after the cells adhere to the wall, replacing with a serum-free culture medium, purifying the cervical intraepithelial neoplasia cells, replacing the culture liquid weekly for at least two times, and after the cells are fused to 80%-85%, carrying out passage. The CIN cells cultured by the method have high survival rate and high purity, allows the morphology of the cells after passage to have no obvious change compared with that of the primary cells, and is carried with human papillomavirus (HPV); and the success of the CIN cell in vitro culture provides technical support for research on treatment of cervical cancer.
Owner:刘玉珍
Preparation method of tissue engineering scar skin model
ActiveCN110331127AAddressing the lack of suitable human scar modelsEpidermal cells/skin cellsArtificial cell constructsScar tissueProtein C
The invention belongs to the technical field of tissue engineering, and provides a preparation method of a tissue-engineered scar skin model. Scar tissue acellular dermal matrix protein, scar fibroblasts and rat tail collagen are subjected to tissue engineering skin culture so as to obtain tissue-engineered skin. The method comprises the following steps: S1, extracting acellular dermal matrix protein of scar tissue; S2, extracting scar fibroblasts; S3, carrying out mixed culture on the scar tissue acellular dermal matrix protein obtained in the S1, the scar fibroblasts obtained in the S2 and the rat tail collagen; and S4, after one week of culture, adding epidermal stem cells, and performing culturing until the tissue-engineered skin is formed. The technical scheme solves the problem of lack of a proper human scar model in the prior art.
Owner:GENERAL HOSPITAL OF PLA
Fixing device used for rat thoracico-abdominal puncturing
InactiveCN106725980AAvoid affecting the experimental resultsHigh transparencyAnimal fetteringSurgical veterinaryEngineeringSurface cover
The invention provides a fixing device used for rat thoracico-abdominal puncturing. The fixing device includes a fixing cover on the upper portion and a base on the lower portion; the upper portion of the base is provided with a rat placement bottom groove in the length direction, and a rat placement top groove is formed in the position, corresponding to the rat placement bottom groove, on the fixing cover; the bottom of the rat placement bottom groove is open, the lower surface of the base is covered with a puncturing net, the base and the fixing cover are fixed through a fixing device body on the base, and the puncturing net is formed by standard meshes with the standard scale. According to the fixing device, based on the size of a rat, the size of rat placement space can be adjusted, and the fixing device is thus suitable for rats different in size; the puncturing net with the standard scale is used for fixing, the thorax and the abdomen can be exposed fully, and according to the standard mesh scale, positioning puncturing is conducted; the fixing device is suitable for rat tail vein blood sampling and other experiments and has multiple functions. The problem that an existing rat abdominal drug delivery fixing device can not prevent rats from turning freely in a fixer and thus drug delivery is affected can be well solved.
Owner:谷翠芝 +2
95 Rifle tactic extension system with laser sight
Owner:丹东依镭社电子科技有限公司
A comprehensive restoration method for dichloroquine phosphate herbicide residues in tobacco fields
InactiveCN104871777BResidue reductionPromote improvementCultivating equipmentsPlant cultivationDiseaseQuinoline
Owner:TOBACCO RES INST CHIN AGRI SCI ACAD +2
Rat tail cutter
InactiveCN108066038AConvenient markingAvoid pollutionAnimal fetteringSurgical veterinaryRat tailEngineering
The invention discloses a rat tail cutter which comprises a box body, a rat tail clip and a cutting blade. Both ends of the box body are provided with openings, and the outer walls of the tops of thetwo ends of the box body are provided with an inlet plate insertion groove and an outlet plate insertion groove respectively. Equidistantly-distributed baffle insertion grooves are formed in the outerwall of the top of the box body, and the baffle insertion grooves are located between the inlet plate insertion groove and the outlet plate insertion groove. The vertical sections of the inlet plateinsertion groove, the outlet plate insertion groove and the baffle insertion grooves each are of a C-shaped structure. An inlet plate is inserted in the inlet plate insertion groove and includes a porous inlet plate and a clamping piece plate. A rat head clamping plate is inserted in the outlet plate insertion groove, and baffles are inserted in the baffle insertion grooves. According to the rat tail cutter, the position of a support can be adjusted according to the length or weight of the rat and the part of the rat to be exposed, an adjustment can be made according to sizes and lengths of different rats, the rat tails and rat heads can be fixed, to facilitate the taking of the rat tail and the marking on the rat ear, the time is saved, and a sample can be protected from pollution.
Owner:SHANGHAI CHEST HOSPITAL
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