74 results about "Spermoblast" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

Pharmaceutical compositions for treating male sub-fertility that include an agent that causes a reduction in an effect of extracellular DNA on sperm cells, are provided. The agent may be, for example, an enzyme that degrades DNA such as DNase, a substance that blocks the interaction between cell free DNA and sperm cell surface receptors, a substance that binds to DNA, a substance that inhibits endogenous sperm cell DNase, a substance that inhibits a member of a signal transduction pathway mediated by DNA binding to sperm cell surface receptors, or an agent that stimulates production of an endogenous substance that causes a reduction in an antifertility effect of cell free DNA on sperm cells. Also provided are methods for treating male sub-fertility by administering the pharmaceutical composition to a subject in need thereof. Further provided are methods for determining a fertility status in a male subject, methods for assisted reproduction, methods for selecting an assisted reproduction technique (ART), and methods for selecting sperm cells in a sperm cell population for use in an assisted reproduction technique.

The invention relates to cell culture media more particularly to fertilisation media, to making and using transgenes, to providing sperm cells for fertilisation particularly in applications such as sperm-mediated gene transfer and to using sperm cells for generating transgenic animals.

The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.

Artificial insemination of female animals and in vitro fertilization of oocytes with fresh or frozen-thawed semen have been applied to the reproduction of animals. According to the traditional theory and conventional procedures, a great number of sperm cells are needed to ensure a successful fertilization.

It is intended to provide a method for improving a development rate in a somatic nuclear transplantation technique or an artificial insemination technique using a spermatid. The method is a method for producing a nuclear-transplanted egg comprising the steps of transplanting a nucleus of a donor cell into an egg, and treating the nuclear-transplanted egg with an anti-methylating agent.

A microfluidic chip is provided for self-sorting highly motile, morphologically normal sperm cell with high DNA integrity from a fresh semen sample. The sperm self-sorting microfluidic chip has one ormore inlet chambers, and sperm collection outlet chamber(s), and the middle of the channel features various micro-fabricated structures in different geometrical shapes and orientations, with varyingperiodicities and patterns, such as an array of micro-fabricated pillars that facilitate the transport of the active and healthy sperm into the outlet chamber.

The invention discloses a method for predicting whether sperms exist in the testis of an azoospermia patient or not according to seminal plasma mRNA. The method comprises the following steps of dividing seminal fluid specimens of the azoospermia patient into a testicular sperm group and a testicular azoospermia group by taking a testicular biopsy as a gold standard, determining the difference of exRNA expression profiles of two seminal fluid groups, carrying out comparison with an existing spermatogenic cell transcriptome database, and searching for differentially expressed exRNA capable of judging spermatogenic cell types, which is used for judging whether haploid sperm cells exist in the testis of the azoospermia patient or not. The clinical application of the scheme provided by the invention can greatly improve the sperm taking success rate of the azoospermia patient, and is also helpful for guiding the azoospermia patient to select an economical, painless and effective treatment method to solve the problem of infertility of the azoospermia patient.

A method for measuring the total sperm concentration (TSC) in a sample is described and includes: (i) placing the sample in a transparent container between a synchronically pulsed light source and a photodetector; and (ii) measuring the optical absorbance of the sample in the range of 800-1000 nm, the TSC of the sample being proportional to the absorbance. Also described is a sampling device for use in optically analyzing a biological fluid, a method for measuring motile sperm concentration (MSC) in a semen sample, a method of determining the average velocity (AV) of sperm cells and a system for analyzing semen quality including means for measuring TSC, means for measuring MSC; and a video visualization system.

The present specification provides for a media composition that provides enhanced viability and activity to sperm cells.

Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic device has at least one flow focusing channel where the components are focused or re-oriented by the geometry of the channel. From an upstream end of the flow focusing channel to a downstream end of the flow focusing channel, at least a portion of the flow focusing channel has a reduction in height and at least a portion of the flow focusing channel narrows in width, thereby geometrically constricting the flow focusing channel. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.

A method for providing semen optimized for use in artificial insemination is described. Methods involve monitoring sperm cell metabolism by assays that produce results in real time, while sperm are still being processed into doses for use in insemination. Processing is modified in response to assay results, to optimize sperm performance.

The present invention discloses a non-human animal model for a hereditary autosomal dominant disease. The non-human animal model expresses at least one phenotype associated with the disease and is obtained by a genetic determinant. The invention also relates to sperm cells and embryos comprising the genetic determinant for an autosomal dominant disease. Furthermore, methods for producing the non-human animal model, sperm cell, and embryos are disclosed.

The embodiment of the invention discloses a sperm fragmentization rate detection method, device and method and a readable storage medium, and the method comprises the steps: obtaining and preprocessing of an archeocyte image; obtaining all kernel image blocks, all first halo image blocks, all cell images and the corresponding relation between the image blocks and the cell images according to the archeocyte image after preprocessing; carrying out the analysis of the kernel image blocks, the corresponding first halo image blocks and the cell images; determining the sperm cells and the sperm kernel image blocks and sperm halo image blocks corresponding to the sperm cells according to an analysis result and the corresponding relation between the image blocks and the cell images; carrying out the analysis of the sperm kernel image blocks and the sperm halo image blocks corresponding to all sperm cells, and judging whether the corresponding sperm cell is qualified or not according to the analysis result; and carrying out the counting of the qualified sperm cells and the unqualified sperm cells, so as to obtain the sperm fragmentization rate. The method improves the work efficiency and the precision of the detection result.

The invention provides a method and a system for acquiring an autosomal STR gene locus typing result of a single individual from mixed seminal stains. The method comprises the following steps of: acquiring different Y-STR gene loci; acquiring autosomal STR gene locus haplotype typing results of a sufficient amount of Y type spermoblast; merging the autosomal STR gene locus haplotype typing results of the Y type spermoblast, which have same typing results of the different Y-STR gene loci to obtain the autosomal STR gene locus typing result of the single individual from the mixed seminal stains. The invention also provides the system for acquiring the autosomal STR gene locus typing result of the single individual from the mixed seminal stains. The method and system which are provided by the invention can acquire the typing result of the single individual from the mixed seminal stains by comprehensively analyzing autosomal STR and Y-STR typing results, thereby providing a powerful technical support for a public security organ to fast determine a criminal suspect.

The present invention discloses a staining mixture including live sperm, a composition that modulates intracellular or extracellular oxidation / reduction reactions, and a DNA-selective dye. Cells contained in such suspensions are more tolerant of multiple steps typically associated with sorting sperm cells into sex-enriched cell populations, thereby increasing the number of viable or motile sperm in the sorted composition. The present invention also discloses a method of staining sperm cells, which includes forming a dye mixture.

A sperm tail recognition method comprises the following steps: step 1, acquiring an image; step 2, acquiring a difference image; step 3, acquiring a tail image; 4, acquiring a sperm head region image;5, combining the tail image and the sperm head region image; and 6, filtering the bright spot area without the long-tail area within a preset distance to obtain a sperm area image. Through acquisition of the difference image, a bright spot area in the semen sample image is filtered out, so that a non-long-tail area is convenient to filter, and an image of impurities or cells, which are greatly different from the sperm structure, in semen is realized. Filtering of bright spot images without long-tail-shaped areas nearby can be achieved through a distance selection method, and then filtering ofbright spot areas of non-sperm cells is achieved. According to the method, impurities in the seminal fluid are accurately filtered, and researchers can count and research the seminal fluid conveniently.

A method for providing semen optimized for use in artificial insemination is described. Methods involve monitoring sperm cell metabolism by assays that produce results in real time, while sperm are still being processed into doses for use in insemination. Processing is modified in response to assay results, to optimize sperm performance.

Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic device has at least one flow focusing channel where the components are focused or re-oriented by the geometry of the channel. From an upstream end of the flow focusing channel to a downstream end of the flow focusing channel, at least a portion of the flow focusing channel has a reduction in height and at least a portion of the flow focusing channel narrows in width, thereby geometrically constricting the flow focusing channel. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.

The present disclosure is directed to methods for typing and characterizing sperm. The technology employs a sequencing-based method for detecting and measuring RNA transcripts from single sperm cells and the analysis of the sequencing data for the prediction of male parent contribution to autism.

A method and system for processing reproductive cell samples and for sorting sperm with reduced levels and occurrences of DNA fragmentation compared to conventional sorting and processing methods, and for using reproductive cell and sperm cell samples with low levels of DNA fragmentation to improve viability of insemination samples fertility and success rates of assisted reproductive procedures, including artificial insemination, in vitro fertilization, intracytoplasmic injection, and other related techniques.

The present invention relates to an immunological method that selects spermatozoa having X-chromosome or Y-chromosome. The method of the invention is based on the use of monoclonal antibodies directed against the genus-specific proteins located in the cytoplasm membrane of spermatozoa associated to the action of the classical complement pathway to increase the percentage of one of the gender in the offspring of mammals.

Provided are methods of in vitro maturation of human spermatogonium, comprising culturing the spermatogonium in a three-dimensional methylcellose culture system (MCS) under conditions capable of differentiating said human spermatogonium into an elongated spermatid, thereby in vitro maturing the human spermatogonium. Also provided is an in vitro matured sperm obtainable according to the method of the invention and methods of treating subjects in need of mature sperm cells.

The invention discloses an application of CLRN3 protein as cell surface identification protein for separating X sperms. The CLRN3 protein is in specific expression on the surface of the mammal X spermoblast, and an amino acid sequence of the CLRN3 protein is as shown in sequence 2 in a sequence table. The method for separating the X sperms provided by the invention is not only simple and easy to do, and the whole separation process is free from using dye and radiation, the pressure on the spermoblast is small, and the harmful influence on the sperm is relatively small. Through the method provided by the invention, the heavy demand on the sexed sperm in the production can be satisfied. The application disclosed by the invention has important application value.

The invention belongs to the technical field of forensic medicine, and particularly relates to a method for recognizing mixed semen stains from two individuals. According to the method, the individuals are separated and recognized by ABH antigen corresponding to ABO blood types of the individuals on spermoblast surfaces. The method particularly includes the steps: 1 preparing the mixed semen stains of the two individuals with ABH antigenic diversity; 2 performing immunohistochemical staining by the aid of labeled A, B and H antibodies, and collecting spermoblast of different ABH blood types bya cell capture technology; 3 performing PCR (polymerase chain reaction) amplification and capillary electrophoresis to perform genetic typing on STR (short tandem repeat) loci. According to the method for recognizing the mixed semen stains from the two individuals, individual origins of DNA (deoxyribonucleic acid) in the mixed semen stains of a plurality of individuals can be effectively determined according to gene level, the step of identifying histologic origins of body fluids or spots on criminal investigation scenes is greatly simplified, and accurate scientific evidence can be providedfor defining case quality, determining criminal suspects, performing conviction and sentencing and the like.

Methods and systems are provided for diagnosing male infertility relating to inadequate production of phosphatidic acid and are complementary to the routine tests, assessing sperm count, motility, viability, head morphology, and white blood cell count. Additional therapeutic methods, compositions and dosage forms are provided for treating male infertility that is related to inadequate production of phosphatidic acid. Such therapeutic approaches involve the use of phosphatidic acid or at least one of its precursors in the sperm intracellular signaling pathway.

Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic devices feature a microfluidic chip having a micro-channel having a constricting portion that narrows in width, and a flow focusing region downstream of the micro-channel. The flow focusing region includes a positively sloping bottom surface that reduces a height of the flow focusing region and sidewalls that taper to reduce a width of the flow focusing region, thereby geometrically constricting the flow focusing region. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.

The invention provides a system (1) for performing sperm analysis and selection based on sperm cell morphology of sperm cells (6) in a fluid (5), the system (1) comprising: (i) a fluid flow channel (2) for transport of said fluid (5), the fluid flow channel (2) comprising an inlet (10) an analyzing zone (40) configured downstream from said inlet (10) and comprising a first pair of electrodes (41) comprising a first intra-electrode distance (dl), a sorting zone (50) configured downstream from said analyzing zone (40) and comprising a sorting device (51), and outlets (80, 90, . . . ) configured downstream from said sorting zone (50); (ii) an electric source (140) configured to provide an electric signal to the first pair of electrodes (41); (iii) a measuring device (150) functionally coupled to the first pair of electrodes (41) and configured to measure a first impedance as a function of time of the fluid (5) between the first pair of electrodes, and to provide time-dependent impedance data; wherein the sorting device (51) is configured to sort sperm cells (6) by directing the sperm cell (6) in the sorting zone (50) to one of the outlets (80, 90, . . . ) based on a comparison in a comparison stage of the time dependent impedance data with predefined reference data.