92 results about "Semen sample" patented technology

A method for increasing the percentage of mammalian offspring of either sex which comprises contacting a semen sample with an antibody specific for the spermatozoa determinative of one sex and separating said spermatozoa from spermatozoa determinative of the other sex, said antibody being bound to a non-porous magnetic bead support having a diameter of 0.1 to 2 microns.

A method for increasing the percentage of mammalian offspring of either sex which comprises contacting a semen sample with an antibody specific for the spermatozoa determinative of one sex and separating said spermatozoa from spermatozoa determinative of the other sex, said antibody being bound to a non-porous magnetic bead support having a diameter of 0.1 to 2 microns.

A jacketed container for holding a semen sample from a mammalian donor at a predetermined temperature for a predetermined period of time prior to incubation to enhance the probability of obtaining offspring of a selected sex through artificial insemination is disclosed. In one embodiment, the semen sample is collected and insulated by a high-heat capacity material that has been preconditioned to a temperature between about 30° C. to about 40° C. before the semen is cooled to a temperature of about 12° C. In a second embodiment, the semen sample is collected and insulated by a high-heat capacity material that has been preconditioned to a temperature between about 4° C. to about 20° C. before the semen is cooled to a temperature of about 12° C.

A method and device for assaying sperm motility in a forward direction and density of active sperm in a semen sample are disclosed. The device includes a microfluidics structure having a sample reservoir, a downstream collection region and a microchannel extending therebetween. The microchannel is dimensioned to confine sample sperm to single-direction movement within the channel, such that sperm in a semen sample placed in the sample reservoir enter and migrate along the microchannel toward and into the collection region. Also included is a detector for detecting the presence of labeled sperm in the microchannel or collection region, and an electronics unit operatively connected to the detector for (i) receiving detector signals, (ii) based on the detector signals received, determining sperm motility and density in the sperm sample, and (iii) displaying information related to sperm motility and density.

A method for collecting and preserving semen of various animals including humans, canines, porcines, bovines, ovines and others involves collecting the semen into a collection vessel where the collection vessel is provided with an extender solution for the semen prior to its collection. Moreover, the extended is preferably maintained at a temperature close to normal body temperature of the species being collected over the time period of its collection. The extender is chosen to buffer the pH of the semen sample and to be isotonic with the semen. The volume of the extender in the collection vessel is preferably chosen such that the semen volume is initially diluted with twice its volume extender solution and some period thereafter the extended semen sample is diluted again at the same ratio. Collection into warmed extender media lessened the cold and pH shock to the spermatozoa, as shown by improved semen parameters. The extender solution is preferably rich in calcium ion. A collection vessel resembling an inverted Y is used for collecting distinct semen samples for comparative study.

Materials and Methods for the separation of X- and Y-chromosome bearing sperm, for example in a semen sample, are provided. The methods involve contacting the semen sample with a binding agent, such as an antibody, that specifically binds to an antigen that is specific for an X- or Y-chromosome. Kits for use in the methods are also provided.

A method and a corresponding composition of matter for diagnosing or increasing the fertility of semen is described. The method involves measuring DNase activity in a semen sample, wherein greater DNase activity in the semen sample indicates increased fertility of the semen sample. Also described is a corresponding composition for storing and increasing the fertility of semen. The composition includes a diluent and an amount of exogenous DNase and-or fertility-associated antigen (FAA) disposed in the diluent. The amount of the exogenous DNase and / or FAA added to the diluent is effective to increase fertility of the semen stored in the composition.

A method for the simultaneous determination of the total concentration of sperm cells and the proportion of live sperm cells in a semen sample is provided. By selective staining of live, dead and / or dying sperm cells, for example using one or more fluorochromes, the detection means can distinguish between live, dead and / or dying sperm cells, for example by detection of the fluorescent response from the sperm cells. The selective staining may be performed at room temperature and in concentrations below standard concentrations. The determination may be performed by selective counting of cells of each fluorescent quality for example by using a flow cytometer having internal concentration standard means. Furthermore, the likelihood of fertilizing a female animal with an insemination dose, taken from a semen sample having a known total concentration of sperm cells and a known proportion of live sperm cells, is predicted on the basis of the thus determined total concentration of sperm cells and the proportion of live sperm cells.

A method for measuring the total sperm concentration (TSC) in a sample is described and includes: (i) placing the sample in a transparent container between a synchronically pulsed light source and a photodetector; and (ii) measuring the optical absorbance of the sample in the range of 800-1000 nm, the TSC of the sample being proportional to the absorbance. Also described is a sampling device for use in optically analyzing a biological fluid, a method for measuring motile sperm concentration (MSC) in a semen sample, a method of determining the average velocity (AV) of sperm cells and a system for analyzing semen quality including means for measuring TSC, means for measuring MSC; and a video visualization system.

A method and device for assaying sperm motility in a forward direction and density of active sperm in a semen sample are disclosed. The device includes a microfluidics structure having a sample reservoir, a downstream collection region and a microchannel extending therebetween. The microchannel is dimensioned to confine sample sperm to single-direction movement within the channel, such that sperm in a semen sample placed in the sample reservoir enter and migrate along the microchannel toward and into the collection region. Also included is a detector for detecting the presence of labeled sperm in the microchannel or collection region, and an electronics unit operatively connected to the detector for (i) receiving detector signals, (ii) based on the detector signals received, determining sperm motility and density in the sperm sample, and (iii) displaying information related to sperm motility and density.

The present invention relates to the field of mammalian reproduction and provides methods, compositions and kits for detecting and assessing spermatozoa and intervening cells in a semen sample which are applicable to human and veterinary uses. Various aspects of the present invention provide for a cytometric multiparametric approach for determining spermatozoa concentration in a semen sample, wherein the cytometric multiparametric approach involves use of one or more spermatozoa-specific detection agents for detection of spermatozoa in the semen sample and one or more intervening cells-specific detection agents.

Systems and methods are provided for evaluating the quality of a semen sample at a mobile device. An assembly includes an optical assembly with at least one lens and a housing configured to engage with the mobile device such that an axis of the optical assembly is substantially aligned with a camera of the mobile device. The optical assembly is contained within the housing. A microfluidic chip includes a reservoir to hold the semen sample. The microfluidic chip engages with the housing such that the reservoir is aligned with the axis of the optical assembly.

A method and device for assaying sperm motility in a forward direction and density of active sperm in a semen sample are disclosed. The device includes a microfluidics structure having a sample reservoir, a downstream collection region and a microchannel extending therebetween. The microchannel is dimensioned to confine sample sperm to single-direction movement within the channel, such that sperm in a semen sample placed in the sample reservoir enter and migrate along the microchannel toward and into the collection region. Also included is a detector for detecting the presence of labeled sperm in the microchannel or collection region, and an electronics unit operatively connected to the detector for (i) receiving detector signals, (ii) based on the detector signals received, determining sperm motility and density in the sperm sample, and (iii) displaying information related to sperm motility and density.

A reagent for detecting acid phosphatase of seminal plasma quantitatively consists of substrate, termination solution, reference material and quality control solution containing acid phosphatase. Its detecting method includes incubating substrate in standard tube synchronously with quality control tube and analysis tube, reacting on quality control solution with substrate and reacting on semen sample in analysis tube with substrate, terminating reacting in quality control tube and in analysis tube, calculating out concentration value of seminal plasma sample according to absorbance of each tube.

The present invention provides methods of determining if an individual is at risk for prostate cancer. The methods measures and compares free and / or bound zinc levels in a semen sample or prostatic fluid, including post massage expressed prostatic fluid, in the potential at risk individual with normal levels. A decrease in zinc level is indicative of a risk for prostate cancer.

Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.

The invention aims to provide an animal semen preservative with biological activity and a preparation method of the animal semen preservative. The animal semen preservative with the biological activity contains oligoprocyanidine so that the animal semen preservative has an anti-oxidation effect, can prolong the preservation time of semen and keeps the activity of animal sperms. The animal semen preservative is prepared by mixing 1000mL of a common medium and 16mg of oligoprocyanidine; the common medium is prepared by mixing 6 parts of glucose, 0.37 part of EDTA (Ethylene Diamine Tetraacetic Acid), 0.375 part of sodium citrate dihydrate, 0-0.12 part of sodium bicarbonate, 0.1 part of neomycin and 100 parts of distilled water; the neomycin is independently added before the semen is diluted. The formula contains the oligoprocyanidine so that the animal semen preservative has the anti-oxidation effect and can prolong the preservation time of the semen; the degradation of a semen sample can be relieved by the oligoprocyanidine and the oligoprocyanidine has no negative effects on the activity of the sperms, so that the activity of the animal sperms is kept; particularly, the survival rate of the sperms preserved at a low temperature is obviously improved.

A method for measuring the total sperm concentration (TSC) in a sample is described and includes: (i) placing the sample in a transparent container between a synchronically pulsed light source and a photodetector; and (ii) measuring the optical absorbance of the sample in the range of 800-1000 nm, the TSC of the sample being proportional to the absorbance. Also described is a sampling device for use in optically analyzing a biological fluid, a method for measuring motile sperm concentration (MSC) in a semen sample, a method of determining the average velocity (AV) of sperm cells and a system for analyzing semen quality including means for measuring TSC, means for measuring MSC; and a video visualization system.

The invention relates to a new method for analysis of a male sperm survival rate. A semen sample is stained by a gentian violet solution at a certain concentration, a staining result is observed under a microscope, survival sperms and dead sperms are determined through colored difference, and the sperm survival rate is calculated; after a same sample is stained by an eosin Y standard staining method recommended by the WHO, the sperm survival rate is calculated; a statistical difference between the two sperm survival rates obtained by the two methods is tested by statistics, a result shows that the gentian violet normal saline solution staining method can be used for distinguishing the dead sperms and the survival sperms, almost all of the sperms are colored after being stained by the gentian violet normal saline, sperm motility is decreased, so as to help to count and carry out subsequent analysis under the microscope. The result proves that the method by using the gentian violet solution to stain the semen sample can easily distinguish the survival sperms and the dead sperms, can further analyze the sperm survival rate, can also assist in the analysis of the sperm motility, and contributes to identification and analysis of male sterility.

In a device to facilitate the collection of semen, a flange is provided for a semen cup, which extends inwardly from the wall of the cup and defines a central opening, thereby providing a peripheral flange to prevent semen from flowing back out and allowing the cup to be held in an inverted or angled orientation while providing the semen sample.

Materials and methods for the separation of X- and Y-chromosome bearing sperm, for example in a semen sample, are provided. The methods involve contacting the semen sample with a binding agent, such as an antibody, that specifically binds to an antigen that is specific for an X- or Y-chromosome. Kits for use in the methods are also provided.

A method of analyzing a semen sample having living sperm is provided comprising receiving from a remote location a set of digital video clips of a prepared semen sample having living sperm and analyzing a subset of the digital video clips taken from the prepared semen sample using a Computer Assisted Semen Analyzer adapted to analyze the subset of digital video clips received from a remote location. A method of measuring sperm morphology is also provided comprising receiving from a remote location a digital video clip of a prepared semen sample having stained non-living sperm, and, analyzing portions of the digital video clip taken from the prepared semen sample.

The invention relates to a raman spectrum-based fast measurement method for a motile sperm deoxyribonucleic acid (DNA). The invention adopts the following technical scheme: collecting a semen sample by a test tube; naturally liquefying semen in a room-temperature environment; absorbing the liquefied semen and adding to a centrifugal tube containing density gradient centrifugate; absorbing upper seminal plasma after centrifuging, discarding, then adding a PBS buffer solution and centrifugally washing again; absorbing supernatant, discarding, adding the PBS buffer solution to prepare a sperm suspension, and incubating; attaching a metal bottom plate to a culture dish; adding the PBS buffer solution, and absorbing the incubated sperm suspension, soaking in the culture dish, wherein the sperm swims outside and contacts the metal bottom plate; setting a spectrum response range, integrating time and the excitation light wavelength, so as to obtain raman spectrum data from the head part of the sperm. The motile sperm swimming out from the pointed-end part of a sucker can be attached to the metal bottom plate by operation of a thin tube pipette, and the damage condition of the sperm DNA is evaluated by measuring the sperm of which the head is attached to the metal bottom plate.

The invention provides a sperm quality detecting instrument and a sperm quality detecting system, which relate to the technical field of in vitro detecting equipment, so as to alleviate or partially alleviate the technical problems of inconvenience in use, low precision and influence on user experience of sperm detecting equipment existing in the prior art, and can improve user experience. The sperm quality detector comprises an image enlarging device, an image collecting device, an image processing device and an image enlarging device, wherein the image enlarging device is used for enlargingthe semen sample to be detected; the image collecting device is used for collecting the image of the enlarged semen sample, generating image information and sending the image information to the imageprocessing device. The image processing device is used for analyzing and calculating the image information to generate a calculation result, and the calculation result includes sperm concentration and / or sperm motility. The sperm quality tester can be used at home, is convenient to use and has higher precision, and allows users to obtain sperm concentration, sperm motility and sperm motility simultaneously through a simpler operation.

The invention relates to a device for collecting semen samples, which is configured such that the patient / donor can perform ejaculation and the corresponding semen collection in his own home, for subsequent transfer to a medical centre or laboratory where the semen can be analyzed. The invention is characterized in that it consists of a preferably plastic cylindrical tube including mouth that is provided with a peripheral flange having a rounded edge in order to allow the condom into which the patient / donor ejaculates to be securely fastened thereto, and a closure cover that is screwed onto the cylindrical tube in order to press and secure the semen-filled condom housed inside said tube.

A sperm liquefying agent is an enzyme preserving fluid. Its preparing method includes adding weighed protein hydrlytic enzyme and carbodiimide into enzyme preserving fluid for reaction of 4-12 hour at 4 degree C, adding 2-aminothanol for reaction of 1-4 hour at room temperature, adding regulatig fluid concenration to be 2-10g / l. A liquefying method is also disclosed.

The invention relates to a reagent and method for increasing the sperm movement speed. The reagent is prepared from progesterone, N6-Monobutyryladenosine-3',5'-cyclic monophosphate and Calcium Ionophore A23187, wherein the concentration ratio of progesterone to N6-Monobutyryladenosine-3',5'-cyclic monophosphate to Calcium Ionophore A23187 is (10-100 microgram / ml):(1-10mM):(1-10mM). The method includes the steps that a sperm culture solution is laid flat on a liquefied semen sample, and after 0.8-1.2 h of upward swimming, liquid at the uppermost end is extracted to obtain capacitated sperms; then, a sterile straw is filled with a sperm culture solution, the reagent is added into the top, the sterile straw is inserted into the upper portion of the capacitated sperms, and after 0.8-1.2 h of upward swimming, liquid at the upper end is collected. By means of the method, the sperm movement speed can be greatly increased, and a theoretical basis is provided for increasing the in-vitro fertilization rate or improving the embryo quality.

A sperm tail recognition method comprises the following steps: step 1, acquiring an image; step 2, acquiring a difference image; step 3, acquiring a tail image; 4, acquiring a sperm head region image;5, combining the tail image and the sperm head region image; and 6, filtering the bright spot area without the long-tail area within a preset distance to obtain a sperm area image. Through acquisition of the difference image, a bright spot area in the semen sample image is filtered out, so that a non-long-tail area is convenient to filter, and an image of impurities or cells, which are greatly different from the sperm structure, in semen is realized. Filtering of bright spot images without long-tail-shaped areas nearby can be achieved through a distance selection method, and then filtering ofbright spot areas of non-sperm cells is achieved. According to the method, impurities in the seminal fluid are accurately filtered, and researchers can count and research the seminal fluid conveniently.

The invention relates to a method for quickly and easily determining whether the semen viscosity of a male is normal or not. According to the method, a glass slide with a certain rough surface is adopted, a semen sample starts to naturally flow from a fixed position of the glass slide with a certain inclining angle under the action of gravity, an operator examines whether the front flowing end of semen exceeds a marking line or not within specified time, and the result can be used for determining whether the viscosity of the semen sample reaches the minimum standard of male fertility or not. Comparing research proves that the determination result of the method has no statistic difference with the result of standard methods, such as a pipette method and a glass rod method, recommended by WHO (World Health Organization), so that the method can be used for quickly examining whether the viscosity of a semen sample is normal or not and assisting in evaluation of male sperm motility. More importantly, an examinee can operate by self at a table by the method to complete the evaluation of semen viscosity, and reference is provided to further screening of infertility.

The invention relates to a method for evaluating male infertility by combining sperm DNA fragmentation index, reactive oxygen species and 8-hydroxydeoxyguanosine. The method comprises the steps of: determining levels of sperm DNA fragmentation index (DFI), reactive oxygen species (ROS) and 8-hydroxydeoxyguanosine (8-PHdG) from semen samples; correlating levels of the sperm DNA fragmentation index,reactive oxygen species and 8-hydroxydeoxyguanosine with sperm function quality. With sperm DFI=19.26%, ROS=1.91ng / ml and 8-OHdG=50.76ng / l as judgment critical values, the sperm is judged to be infertile when the sperm DFI, ROS and 8-OHdG levels are higher than the critical values.