31 results about "Stem elongation" patented technology

The invention provides a fast and efficient propagation method for forming bulbils through in-vitro induction of stem segments of lilium lancifolium. The method comprises the steps of (1) precooling treatment of seed bulbs; (2) elongation cultivation of the stems; (3) bulbil formation through induction of the stems, bulbil expansion and root taking. A lilium lancifolium stem elongation culture medium is prepared from MS, 30 g / L saccharose and 5.0 g / L agar; a bulbil induction, expansion and root taking culture medium is prepared from MS, 1.0 mg / L 6-BA, 30 g / L saccharose and 5.0 g / L agar. By means of the propagation method for forming the bulbils through in-vitro induction of the stems of the lilium lancifolium, each elongated stem can generate about 20 or more nodal axillae, each axillae can form 2-3 bulbils, the propagation coefficient of the lilium lancifolium is significantly increased, and the propagation efficiency of the lilium lancifolium is significantly improved. Compared withthe process of forming bulbils and directly inducing multiple shoots to re-generate bulblets through scales under a natural condition, the culturing period is greatly shortened, the steps are simplified, and the method has high practical application value.

The embodiment of the invention relates to the technical field of farming and discloses a cucumber seeding raising method. The cucumber seeding raising method comprises the steps: sowing seeds in a planting substrate; monitoring the growth stages of the seeds planted in the planting substrate, wherein the growth stages include a seed germination stage, a cotyledon and stem elongation stage, an euphylla growth stage and a seedling acclimatization stage; and controlling the water content of the planting substrate according to the current growth stage of the monitored seeds, wherein the water content of the planting substrate at the seed germination stage is controlled at 80%-90%, the water content of the planting substrate at the cotyledon and stem elongation stage is controlled at 60%-70%,the water content of the planting substrate at the euphylla growth stage is controlled at 70%-80%, and the water content of the planting substrate at the seedling acclimatization stage is controlled at 70%-80%. The cucumber seeding raising method can be used for reducing the problems of low spindling seedling and germination rates of cucumbers and irregular grown seedlings and is beneficial to thecultivation of robust cucumbers.

An object of the present invention is mainly to provide, using a genetically engineering technique, a plant with enhanced root elongation; a plant immune to suppression in root elongation ability even under hyperosmotic stress; and a method for enhancing plant root elongation. The subject invention produces a transgenic plant in which a Ran protein derived from a wild watermelon (Citrullus lanatus) is expressed, using a genetically engineering technique.

The invention discloses an organic cultivation method for gramineous pasture in alpine pastoral areas. The organic cultivation method comprises the steps of land selection, soil preparation and fertilizer application, seed selection and planting, filed management, and harvest after maturity; the step of land selection comprises selecting a land parcel with organic certification or an organic certification conversion period, wherein the land parcel is a complete land parcel containing no land parcel of conventional production; the step of soil preparation and fertilizer application comprises uniformly applying a base fertilizer on the ground surface of the selected land parcel, and carrying out soil deep tillage and soil airing on the soil of the land parcel; the step of seed selection andplanting comprises applying a seed fertilizer during a sowing process, and applying the seed fertilizer by using a seed-fertilizer layering sowing machine; and the step of field management comprises applying topdressing before the stem elongation of the pasture, wherein the base fertilizer, the seed fertilizer and the topdressing are selected from farmyard manure, biological organic fertilizers ora combination of the farmyard manure and the biological organic fertilizers, the farmyard manure is prepared by composting and decomposing animal manure, livestock and poultry manure, green manure and straw, and the biological organic fertilizer are approved by a certificate authority.

The invention discloses a histone demethylase gene JMJ703 for regulating a rice stem height, and applications thereof. According to the present invention, one of histone demethylases and gene JMJ703 thereof are selected through searching in a chromatin protein database, wherein the full-length cDNA accession number is AK121381, a gene ID number is 4338043, a prediction coding region comprises 3717 bp, and 1238 amino acids are coded; a RNA interference vector of the gene is transformed into rice through a RNA interference method, and results show that a stem height of the transgenic plant is shorter than a stem height of the wild type while a length of the leaf has no apparent change so as to improve a column shape of the rice; tobacco heterogenesis in vivo test results show that JMJ703 protein is demethylase of histone H3K4me1 / H3K4me2 / H3K4me3; and the JMJ703 is functional histone demethylase gene and can be provided for regulating a stem elongation process of the rice, wherein the stem of the rice expressed after gene inhibition becomes short and a characteristic of lodging resistance is provided while developments of leaves and other organs are normal so as to provide a selective plant type for breeding.

The invention discloses an improved field trail test for assessment of sugarcane hybridized combinations. Seedlings are cultivated in a seedling cultivation greenhouse in or around middle April everyyear and temporarily planted by a row spacing of 10cm and a line spacing of 20cm in an outdoor seedbed in or around middle July to form seed stems through stem elongation on the seedbed, and then theseed stems are cut during January to March and planted in a field by a line length of 1.5m and a line spacing of 1m, each line allows planting of one clone, 8 buds of each clone are planted, and eachcombination is planted for three times repeatedly; at the end of the year or the beginning of the next year, agronomic characters and disease symptoms required by combination assessment are collected,and advantages and disadvantages of the combinations are assessed through statistic analysis to provide references for hybridized combination preparation and variety breeding. By adoption of the method, objective trail results can be obtained, reliability of assessment results of the hybridized combinations can be improved, and increasing of sugarcane breeding efficiency is further promoted.

The invention discloses an application method of a chemical fertilizer in the fertilization reduction synergy of oilseed rape. The application method comprises the following steps: A, the type and the application amount of fertilizers are determined: the oilseed rape fertilizing amount is the total fertilizing amount of a nitrogen fertilizer (by pure nitrogen), a phosphorus fertilizer (by phosphoric pentoxide) and a potassium fertilizer (by potassium oxide), the use amount of the chemical fertilizer urea and the use amount of a nitrogen fertilizer synergist are calculated according to the total nitrogen amount of the chemical fertilizer, the use amount of a phosphorus activator is calculated according to the oilseed rape field plantation area, and the total phosphorus and potassium amount of the organic fertilizer is calculated; B, the organic fertilizer, the phosphorous fertilizer, the potassium fertilizer, the nitrogen fertilizer synergist and the phosphorus activator are uniformly once applied as a base fertilizer; C, the nitrogen fertilizer is applied in three stages, the phosphorus fertilizer and the potassium are once applied as a base fertilizer, a use amount ratio of the nitrogen fertilizer base fertilizer to a seedling fertilizer to a stem elongation fertilizer is (4-7):(1-3):(1-3), the base fertilizer is applied in furrows, the seedling fertilizer is applied in 10-40 days after oilseed rape seedlings are transplanted, and the stem elongation fertilizer is applied in 50-100 days after the oilseed rape seedlings are transplanted; and D, fertilization is not carried out in the post-emergence stage, the flowering stage or the maturation stage. The method has the advantages of easiness in implementation, simplicity in operation, reduction of the application of the chemical fertilizer, improvement of the quality of soil through applying the organic fertilizer, improvement of the nutrient utilization efficiency and increase of the yield of the oilseed rape.

The invention discloses a biology-fertigation combined restoration method for medium-and-mild cadmium-polluted soil. The method comprises the following steps: step (1) selecting a silage corn variety,carrying out basal application by using an acidic fertilizer, carrying out irrigation by using emergence water, and carrying out sowing according to a sowing amount of 2.5-2.8 kg per mu; step (2), carrying out top dressing by using the acidic fertilizer in a stem elongation stage; step (3), carrying out fallowing for 7-10 days after silage corns are harvested, and carrying out rotary tillage during the fallowing period; step (4) selecting a winter wheat variety, carrying out basal application by using an alkalescent fertilizer, carrying out irrigation by using the emergence water, and carrying out sowing according to a sowing amount of 12-14 kg per mu; step (5) carrying out irrigation by using clear water in a heading and filling period of winter wheat; and step (6) carrying out fallowingfor 3-5 days after the winter wheat is harvested, and carrying out rotary tillage during the fallowing period. According to the method provided by the invention, basal application is carried out by using the acid fertilizer before sowing the silage corns, and topdressing is carried out by using the acidic fertilizer in the stem elongation stage, so that the cadmium-carrying capacity of corn stalks can be improved, and the soil restoration period can be accelerated.

The invention discloses a method for increasing the producing quantity of regenerative water bamboos of single-cropping water bamboos. The method comprises the following steps: increasing the stem elongation height, keeping leaves and harvesting water bamboos, and producing regenerative water bamboos. The method has the beneficial effects that rotten stems of water bamboos can be effectively decreased and the producing quantity of the regenerative water bamboos can be increased by adopting manners of increasing the stem elongation height and keeping leaves and harvesting water bamboos, so that the effect of greatly increasing the yield of the second-cropping single-cropping water bamboos can be realized, the use effect is ideal, and the method is beneficial to popularization on production.

The present invention relates to a flowering-time and / or stem elongation regulator isolated from rice, which is selected from OsMADS50, OsMADSS1, OsMADS56, OsMADS14, OsTRX1, OsVIN1, OsCOL4 and OsCOLS, a DNA construct containing the regulator, a transgenic plant, a part thereof, and plant cell transformed with the DNA construct, and method to control flowering-time and / or stem elongation using the regulator. In the present invention, the flowering-time and / or stem elongation can be controlled, and thereby, various agricultural benefits obtained.

An object of the present invention is mainly to provide, using a genetically engineering technique, a plant with enhanced root elongation; a plant immune to suppression in root elongation ability even under hyperosmotic stress; and a method for enhancing plant root elongation. The subject invention produces a transgenic plant in which a Ran protein derived from a wild watermelon (Citrullus lanatus) is expressed, using a genetically engineering technique.

The invention provides a direct-sowing planting method used for rice in the arid land and based on a metal drought-resistant agent. The method comprises steps as follows: field management: the loose soil layer is made to be 15-20 cm through mechanical rotary tillage, bottom fertilizer is applied, the land is raked to be flat, and the water content of soil is 60%-70%; sowing: rice seeds are manually sown in drill at the row spacing of 25-30 cm, and the sowing rate is 60-70 kg / hm<2>; water management: after seedlings of rice come out, first water irrigation is performed, the metal drought-resistant agent is sprayed for irrigation, a rain-fed mode is taken as a primary at other time, and irrigation is performed only when the water potential of the soil is lower than minus 50 kPa; fertilizer management: the total application amount of pure nitrogen is 270 kg / hm<2>, the ratio of base fertilizer, tillering fertilizer and earing fertilizer is 2:4:4, the tillering fertilizer is applied in a one-tip three-leaf stage, the ratio of nitrogen, phosphorus and potassium is 2:1:1, phosphorus fertilizer is subjected to one-time basal application, and potassium fertilizer is applied before ploughing and at the stage of stem elongation in equal amount; control management of diseases, pests and weeds; harvesting. According to the sowing method for rice in the arid land, the metal drought-resistant agent containing calcium, magnesium, copper and iron is used at little dosage, has a good effect and is low in cost and friendly for the environment and human bodies.

The invention relates to the technical field of insect breeding, in particular to a chilo suppressalis breeding method. An egg mass is positioned at the intersection of a sheath of a first rice stem and a main stem, and hatched larvae bore through the first rice stem to eat; after the larvae grow to an age of 5, the larvae are taken out from the first rice stem and fed on a second rice stem, wherein the first rice stem refers to a fresh rice stem with roots and the sheath in or after a round stem elongation stage, and the second rice stem refers to a knotted fresh rice stem in or after the round stem elongation stage. The chilo suppressalis breeding method has advantages of high larva and pupa survival rate, convenience in pupa searching, suitableness for researching and availability for large-scale breeding.

The invention discloses a method for reducing the cadmium content of corns through carrying out intercropping with sonchus asper and coupling with passivating agents. The method comprises an intercropping mode of planting corns on ridges and planting sonchus asper in ditches between every two adjacent ridges. A passivating agent LH or a passivating agent LS or a passivating agent pH is applied while a base fertilizer is applied to the corn planting ridges, wherein the passivation agent LH is 150 kg / mu of lime and 1500 kg / mu of humic acid, the passivating agent LS is 150 kg / mu of lime and 1500kg / mu of sepiolite, and the passivation agent pH is 100 kg / mu of calcium-magnesium-phosphate fertilizers and 1500 kg / mu of humic acid. In a stem elongation period of the corns, a phosphatic fertilizerand a potash fertilizer are applied to the corns, and only a base fertilizer is applied to the sonchus asper. According to the method, the corn yield and the farmer income are ensured, and compared with single cropping of corns without application of a passivating agent, single cropping of corns with application of a passivating agent, and interplanting of corns with sonchus asper, the method provided by the invention has the advantages that the pollution caused by the Cd content of corn grains is greatly reduced, so that the ecological environment is protected.

The invention discloses a planting method for barley. Directed at the disadvantages in the prior art, the objective of the invention is to provide the planting method for the barley. The method comprises the following seven steps: soil selection and sowing; sowing time and preparation before sowing; sowing; irrigation for moisture conservation when ice melts and spring begins in the next year; irrigation for moisture conservation, stem elongation and grain-filling in the production of the barley; applying of chlormequat chloride when the barley grows at a rate of more than 1 cm every week; and harvesting. The planting method for the barley provided by the invention can substantially improve the yield of the barley and increases drought-resistant and lodging-resistant capabilities of the barley.

The invention discloses a method for promoting stem elongation in cherry blossom in vitro culture, and belongs to the technical field of plant tissue culture. A method for promoting stem elongation in cherry blossom in vitro culture disclosed by the present invention, through sterilizing explants, cold treatment and culturing under red light; overcomes the short growth of cherry blossom seedlings and the obstruction of stem elongation in the process of tissue culture problems; use low temperature to break the dormancy of buds, use hormones and red light to promote stem growth, and the length of stem segments reaches 4.23cm.

The invention belongs to the technical field of sugarcane production research, and particularly relates to a method for analyzing influence of different meteorological factors on sugarcane growth, which comprises the following steps of: selecting a test field: selecting a rain-fed sugarcane field which is not subjected to irrigation management in a whole-year sugarcane planting period because the soil texture type of the test field is clay loam, neutral and medium in fertility; data observation: starting to measure the sugarcane in the test site from the sugarcane stem elongation period and from the flood end of the current ten-day period of the sugarcane stem elongation period; and data statistics: starting from a sugarcane stem elongation stage, observing and counting meteorological observation data through an agricultural meteorological observation station, and carrying out correlation analysis by taking the meteorological observation data as an independent variable and taking the elongation of the sugarcane as a dependent variable. According to the method, the influence of the meteorological factors on the plant height growth in one production cycle of the sugarcane is researched, the relation between the meteorological factors and the sugarcane elongation is analyzed through correlation, a reliable theoretical basis is provided for the intelligent development of the sugarcane, and the method has important significance in promoting the planting and production of the sugarcane.

The present invention discloses an organic selenium enriched seasoning material for hotpots. The organic selenium enriched seasoning material for the hotpots comprises the following components in weight percentages: 20-30% of a sweet flour paste, 3-5% of dark soy sauce, 4-8% of organic selenium enriched sucrose, 1-2% of dried tangerine peels, 2-8% of edible salt, 0.5-1% of monosodium glutamate, 2-5% of honeysuckle and the balance water. The organic selenium enriched sucrose is added into the seasoning material, health-care and food therapy effects of the seasoning material are increased, the dried tangerine peels have functions of regulating qi and promoting digestion and at the same time are liable to cause internal heat, and an addition of the honeysuckle can enhance an effect of promoting digestion and can also ease internal heat symptoms. A preparation method of the organic selenium enriched sucrose overcomes a problem that a conversion and utilization rate of the sucrose for inorganic selenium salts is low in traditional methods. A method of spraying an inorganic selenium salt solution on leaf blades of sugarcanes in a growth and stem elongation stage of the sugarcanes can significantly improve contents of the organic selenium in the sucrose. The prepared organic selenium enriched sucrose is low in costs and simple in the method.

The invention discloses a method for enhancing the stress resistance by applying a soil activator to a medium indica rice field, and relates to the technical field of crop cultivation. The method comprises the following steps: at the 50th day after a medium indica rice machine transplants rice, after indica rice is subjected to stem elongation and before the booting stage, building a water pond of 0.2 m<3> on the side of the medium indica rice field, covering the bottom of the water pond with a plastic thin film, then injecting 0.2 m<3> of river water into the film-covered water pond, feeding 4 kg of brown sugar and 2 kg of the soil activator into the water pond, and uniformly stirring the materials for fermentation for 24 hours, thus obtaining mother liquid; adding water into 200 kg of the mother liquid for dilution till the total weight is 2,000 kg, and uniformly sprinkling the diluent into the medium indica rice field of 2 hectares; after one month, performing secondary application of the same diluent. According to the method, the soil activator is applied to the medium indica rice field to realize structural modification for micropopulations and nutrients in the soil and enhance the stress resistance of rice plants in the middle and later periods. The pond is built on the side of the rice field for fermentation, thus producing the mother liquid; by mixing of the soil activator and the brown sugar for fermentation, the improvement effect of the soil activator on the soil in the medium indica rice field can be obviously enhanced.

The invention discloses a package fertilizer for annual simplified and reduced fertilization of continuous double cropping of cotton and rape. The package fertilizer comprises a base fertilizer which is applied at the same time with seed sowing at one time and a water-soluble fertilizer for foliage spraying in seedling and bud stages-initial flowering stage, blossing and boll-forming stages and rape seedling stage-stem elongation stage, and flowering stage, wherein the base fertilizer which is applied at the same time with the seed sowing at one time comprise the following effective elements based on pure nutrients in percentage by weight: 16-24% of N, 5-8% of P2O5, 15-25% of K2O, and 3-5% of S; a water-soluble fertilizer formula comprise the following effective elements based on pure nutrients: B, Zn, N, P2O5, K2O, amino acid, humic acid, and oligosaccharins. The package fertilizer provided by the invention can effectively ensure the nutrient demands of the cotton and the rape throughout the growth period, and has the functions of preventing diseases, preventing boll abscission, preventing rotten bolls, resisting high temperature and the like; the fertilization procedure is simplified; the use amount of chemical fertilizers is reduced; fertilizer utilization rate and fertilization benefits are improved.

The invention discloses a method for increasing the producing quantity of regenerative water bamboos of single-cropping water bamboos. The method comprises the following steps: increasing the stem elongation height, keeping leaves and harvesting water bamboos, and producing regenerative water bamboos. The method has the beneficial effects that rotten stems of water bamboos can be effectively decreased and the producing quantity of the regenerative water bamboos can be increased by adopting manners of increasing the stem elongation height and keeping leaves and harvesting water bamboos, so that the effect of greatly increasing the yield of the second-cropping single-cropping water bamboos can be realized, the use effect is ideal, and the method is beneficial to popularization on production.

The invention discloses a histone demethylase gene JMJ703 for regulating a rice stem height, and applications thereof. According to the present invention, one of histone demethylases and gene JMJ703 thereof are selected through searching in a chromatin protein database, wherein the full-length cDNA accession number is AK121381, a gene ID number is 4338043, a prediction coding region comprises 3717 bp, and 1238 amino acids are coded; a RNA interference vector of the gene is transformed into rice through a RNA interference method, and results show that a stem height of the transgenic plant is shorter than a stem height of the wild type while a length of the leaf has no apparent change so as to improve a column shape of the rice; tobacco heterogenesis in vivo test results show that JMJ703 protein is demethylase of histone H3K4me1 / H3K4me2 / H3K4me3; and the JMJ703 is functional histone demethylase gene and can be provided for regulating a stem elongation process of the rice, wherein the stem of the rice expressed after gene inhibition becomes short and a characteristic of lodging resistance is provided while developments of leaves and other organs are normal so as to provide a selective plant type for breeding.

The invention provides a nitrogen application method for balancing the relation between supply and demand of nitrogen in intercropping of peas and corn. The nitrogen application method comprises the steps as follows: S1, intercropping planting of corn and peas is performed in the line number ratio of (2-3):4, and the line width ratio of corn belts to pea belts is (8-11):8; basic pure nitrogen is applied to the corn belts and the pea belts, pure phosphorus is applied, turning is performed after fertilization, and the corn belts are covered with mulching films; S2, pure nitrogen is dressed on the corn belts and the pea belts for the first time at the stem elongation stage of corn; S3, pure nitrogen is dressed on the corn belts and the pea belts for the second time at the flare opening stageof corn; S4, pure nitrogen is dressed on the corn belts for the third time 10 days after blooming of corn, and no fertilizer is applied to the pea belts. Nitrogen is supplied at different stages and belts according to nitrogen demand laws of the corn and the peas, interspecific competition of corn and peas is regulated, and the problem of imbalance between supply and demand of nitrogen in traditional production of intercropping of peas and corn is effectively solved; meanwhile, by regulation of interspecific competition and complementation of an intercropping system, the synergistic utilization of nitrogen is promoted, the utilization rate of nitrogen fertilizer is increased, and the yield is increased.

The invention belongs to the technical field of rape cultivation, and particularly relates to a method for increasing the oil content of Brassica napus rapeseeds and improving lodging resistance simultaneously and application of the method. According to the method, in the early stage of the stem elongation period of Brassica napus rapes, the bolting height is 8-12 cm, a liquid silicon fertilizer aqueous solution with the monosilicic acid content being 0.086-0.101g.L<-1> is used between December 20th to January 20th next year in Gregorian calendar time, and the oil content of the Brassica napus rapeseeds is increased and lodging resistance is improved simultaneously. The method is applicable to Brassica napus rape varieties in rape production areas in the Yangtze river basin. By means of the method, the oil content is increased and lodging resistance is improved simultaneously under the condition that the yield of the Brassica napus rapes is higher through high-nitrogen culture, so that quality of the rapes is improved easily, application and popularization of the mechanical harvesting technology are facilitated, and economic benefits of rape production are improved.

The invention belongs to the technical field of rape cultivation, and particularly relates to a method for increasing the oil content of Brassica napus rapeseeds and improving lodging resistance simultaneously and application of the method. According to the method, in the early stage of the stem elongation period of Brassica napus rapes, the bolting height is 8-12 cm, a liquid silicon fertilizer aqueous solution with the monosilicic acid content being 0.086-0.101g.L<-1> is used between December 20th to January 20th next year in Gregorian calendar time, and the oil content of the Brassica napus rapeseeds is increased and lodging resistance is improved simultaneously. The method is applicable to Brassica napus rape varieties in rape production areas in the Yangtze river basin. By means of the method, the oil content is increased and lodging resistance is improved simultaneously under the condition that the yield of the Brassica napus rapes is higher through high-nitrogen culture, so that quality of the rapes is improved easily, application and popularization of the mechanical harvesting technology are facilitated, and economic benefits of rape production are improved.

The invention discloses a tissue culture rapid propagation method of a new variety 'Shirley' of a vertical branch type chionanthus retusus. The method comprises the following steps of (1) cutting a tender stem with the length of 2-2.5 cm from a 'Shirley' stock plant as a test material, inoculating the test material into a culture medium for inducing stem differentiation, and inducing adventitiousbud differentiation and proliferation; (2) when the test material in the step (1) has 4-5 new leaves and is differentiated to form 1-2 adventitious buds, transferring the test material into the culture medium for inducing stem elongation so as to promote stem elongation; (3) when the stem of the test material in the step (2) elongates to 3-5cm, transferring the stem into a rooting culture medium,and carrying out adventitious root induction; and (4) hardening and transplanting rooted seedlings. When the method disclosed by the invention is used for propagating the 'Shirley', the new variety 'Shirley' can be popularized in a short time, so that the new variety 'Shirley' can be rapidly applied to greening practice. A theoretical basis is provided for establishing a rapid propagation system with stable and efficient chionanthus retusus characters and a subsequent genetic transformation experiment.

The invention discloses a foliar fertilizer special for spring rape. The foliar fertilizer is prepared from the following raw material components: urea, ammonium polyphosphate, monopotassium phosphate, chitosan oligosaccharide, EDTA (Ethylene Diamine Tetraacetic Acid) chelated magnesium, EDTA chelated zinc, EDTA chelated calcium and boric acid. Aiming at the characteristics that the spring rape grows quickly from the seedling stage to the stem elongation stage and the nutrient demand intensity is relatively high, the special foliar fertilizer for the spring rape is prepared by reasonably matching the components such as macronutrient elements, medium and trace nutrient elements, a plant growth regulator and the like according to the nutrient demand characteristics of the spring rape in the seedling stage; the fertilizer can be rapidly absorbed and utilized by plants after being sprayed in the spring rape seedling stage, formation of branches and siliques of the spring rape is remarkably promoted, finally the yield and quality of the spring rape are remarkably improved, and the fertilizer utilization rate is high; and meanwhile, the special foliar fertilizer for spring rape is simple in preparation method, low in cost, convenient to apply and suitable for large-scale mechanical operation.

In this study, we used a wound-inducible promoter of the broccoli (Brassica oleracea var. italica) GLUCOSE INHIBITION of ROOT ELONGATION1 (GIR1) gene fused to β-glucuronidase (GUS, pBoGIR1::GUS) as a selectable marker. Transgenic broccoli plants expressing pBoGIR1::GUS appear blue in planta at wounded regions after GUS staining for 30 min. Similarly, the blue color is visible in transgenic Arabidopsis and rice plants expressing pBoGIR1::GUS at wounded areas after GUS staining for 2 h, indicating that this promoter is wound-inducible in both dicots and monocots. GUS staining is very rapid and the partial wounding in this study is a nondestructive method that does not affect further plant growth and development. Thus, pBoGIR1::GUS could serve as an effective substitute for antibiotic- and herbicide-resistance genes in the generation of genetically modified crops.