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32 results about "Trout/salmon" patented technology
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Various fish of the family Salmonidae.
All female method for salmon and bulltrout
The present invention relates to a gender artificial-control technique of the cultivated fish, in particular to a gender control technique of the all-female-orientation of the salmon and bulltrout. Using the estrogen hormone to feed the normal fry transforms the male fry to be a fake-male fish with the female shape feature and the male physiological feature; using the sperm of the fake-male fish to fertilize the normal egg can realize that all offspring are female. When the fries of the salmon and bulltrout start to take food, the fries are fed with the feed containing methyl testosterone for three to six months and then the fries are fed with normal feed until the sex maturation. Selecting the fake-male fish with a female external morphological feature and a sex gland of the spermarium, taking out the spermarium, grinding the spermarium with a mortar, diluting the spermarium powder with the conventional sperm dilution, nursing the solution inside a refrigerator in four cent degrees for two to four hours, and then mating with the male fish to produce the all-female salmon and bulltrout. All fish output by the present invention are female; when in maturation, the ovary of the fish contains a plurality of fish roes that can be make into a featured dish or processed into the caviar, thereby improving the cultivation benefit.
Owner:白宝海
Breeding and hatching device for salmon and trout
InactiveCN102047850AExchange evenlyIncrease dissolved oxygenClimate change adaptationPisciculture and aquariaOzone generatorOrganic glass
The invention discloses a breeding and hatching device for salmon and trout. The salmon and the trout comprise chum salmon, salmon, golden trout, rainbow trout and the like, and have delicious meat and a wide development prospect, and particularly eggs of the salmon and the trout have high nutritional value and economic value. Most of the eggs are demersal and have large granules, and the conventional organic glass cylinder hatcher and glass reinforced plastic tank hatcher cannot meet the requirements of hatching and breeding the salmon and the trout. The breeding and hatching device for the salmon and the trout comprises a support (1) which is provided with a rail, wherein the rail is connected with a rail sliding block (2) which supports a hatching tank (3); a water spray pipe (4) and a water supply pipe (5) are arranged above the hatching tank (3); a support plate (12) is arranged below the hatching tank (3); an ultraviolet lamp ozone generator (6) is arranged below the support plate (12); and a circulating water tank (7) is arranged below the ultraviolet lamp ozone generator (6). The breeding and hatching device is used for breeding and hatching the salmon and the trout.
Owner:XINJIANG YIHE STURGEON IND TECH CO LTD
Method for marking salmon and trout by daily growth increments in otoliths
InactiveCN101496502AGuaranteed accuracyReduce marking costsClimate change adaptationPisciculture and aquariaOtolithIncubation period
The invention relates to an otolith daily increment labeling method for game fish, and relates to an otolith daily increment labeling method for fish. The invention solves the problems that: the prior labeling method takes time and labor, and has expensive labeling cost, harm to fish bodies and complex operation; and the label is easily lost and the labeling quantity is limited. The method comprises the following steps: controlling the water body temperature in the game fish incubation period so as to change the otolith daily increment appearance feature of the game fish and form an recognizable game fish otolith daily increment label; and setting the water body temperature in the incubation period 2 to 4 DEG C higher or lower than the normal water temperature in the game fish incubation period. The method has the advantages of time and labor reduction, safety, simple operation, lifelong reading, no harm to the fish bodies, and a large quantity of labels. According to the quantity and category of the labeled fish, the process and the method with different labeling spectrums are designed. The application of the method can reach 100 percent of colony labeling.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Method for inducing hucho taimen young fishes to open mouths and to be domesticated
ActiveCN103907551AImprove survival rateImprove palatabilityClimate change adaptationAnimal feeding stuffKrillPollution
The invention discloses a method for inducing hucho taimen young fishes to open mouths and to be domesticated. The method includes the steps: stabilizing the young fishes for 1-2 days and then pouring the young fishes into a disinfected hatching tank by a bolting silk after the young fishes completely float in a floating management period; mixing trout feed and krill powder according to the ratio of 2-3:1, feeding the young fishes for 4-5 days, changing the mixing ratio into 3-4:1, feeding the young fishes for 3-4 days, changing the mixing ratio into 6-8:1 and feeding the young fishes for 2-3 days in a food calling and feeding period; feeding the young fishes with 0.6-0.8mm trout feed for 3-4 days at a first stage, training the young fishes in scrambling for taking food, feeding the young fishes for 3-4 days at a second stage and training the food intake rhythm of the young fishes scrambling for pure total artificial feed in a total artificial feed feeding period. The method can solve the problem that large-scale domestication of the hucho taimen young fishes lack easily preserved conventional baits with a wide source and high palatability and without pathogenic pollution.
Owner:BEIJING FISHERIES RES INST
Parent fish rearing method for gymnocypris potanini
InactiveCN106508747AImprove hatchabilityImprove sperm and egg qualityClimate change adaptationPisciculture and aquariaBroodstockReproduction
The invention discloses a parent fish rearing method for gymnocypris potanini. The method comprises the steps that A, standby parent fishes are selected, wherein fishes without surface wounds are selected as the standby parent fishes; B, the parent fishes are put, wherein the parent fishes are put in breeding buckets, and the breeding buckets are disinfected with potassium permanganate; C, temporary rearing is carried out, wherein wild standby parent fishes are put in the buckets and are not fed; D, eating training is carried out, wherein water earthworms and feed special for salmon and bulltrout are mixed to be uniform for eating training; E, breeding is carried out, wherein fishes fished in anestrous seasons are bred; F, the parent fishes are selected, wherein before the anestrous seasons, the developed parent fishes are selected for gonad reinforcement breeding, and feed, water flow and male and female mixed culture are included; G, artificial reproduction is carried out, wherein the parent fishes stops being fed before artificial reproduction is carried out, the parent fishes are fished out, and the female fishes developed well are selected for artificial reproduction. The method is easy to implement and easy and convenient to operate, the parent fishes which are mature, large in brood amount, high in reproduction capacity and high in sperm and egg quality are obtained, and synchronous development of the male parent fishes and the female parent fishes is achieved.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
Production system and method for puffed feed for salmons and trouts
ActiveCN112262932AGood ripening uniformityBeautiful materialFeeding-stuffWorking-up animal fodderProcess engineeringFodder
The invention relates to a production system and method for a puffed feed for salmons and trouts, and belongs to the technical field of feed preparation. The production system for the puffed feed forsalmons and trouts comprises a twin-screw puffing mechanism, a stirring tank, a vacuum spraying mechanism and a stirring mechanism, wherein the twin-screw puffing mechanism is mounted at the top of the stirring tank, is provided with a feeding opening and a discharging opening, and communicates with the top of the stirring tank through the discharging opening; a vacuum pumping opening is formed inthe side wall of the stirring tank; the vacuum spraying mechanism is mounted on the top wall of the stirring tank; the stirring mechanism is mounted in the stirring tank; and a particle outlet is formed in the bottom of the stirring tank. The production system disclosed by the invention is simple in structure and convenient to assemble, has the functions of twin-screw puffing, extruding, discharging and vacuum spraying of grease, and is suitable for popularization and use.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Salmo trutta fario linnaeus parent fish feed with low protein and amino acid balance
The invention discloses a salmo trutta fario linnaeus parent fish feed with low protein and amino acid balance, which belongs to the technical field of aquatic feed products, and can be used for solving the problems that no salmo trutta fario linnaeus feed exists and the protein conversion rate is low and the amino acid is unbalanced when a trout feed is used. The salmo trutta fario linnaeus parent fish feed comprises wheat middling, soybean protein concentrate, fish meal, fish oil, soybean soil, cellulose, calcium dihydrogen phoshate, inkfish paste, soyabean lecithin, ethoxyquin, arginine, methionine, phenylalanine and a premix. According to the salmo trutta fario linnaeus parent fish feed, the utilization rate of a feed raw material with lower digestibility of salmo trutta fario linnaeus is reduced, the quality of the salmo trutta fario linnaeus parent fish feed is easily controlled, the digestion utilization rate of the salmo trutta fario linnaeus parent fish feed is improved, a raw material source is stable, the cost is lowered, the problem of unbalanced amino acid of a conventional feed is overcome, the protein efficiency of the feed is favorably improved, the emission of ammonia nitrogen is reduced, the pollution to a water environment is alleviated, the anti-stress ability and the immunity of a salmo trutta fario linnaeus parent fish are improved, and the quality of sperm eggs is improved.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Atlantic salmon DNA bar code standard detection gene, and primer and application thereof
InactiveCN112662784AAccurate identificationAccurate identification methodMicrobiological testing/measurementDNA/RNA fragmentationNucleotideNucleotide sequencing
The invention relates to an Atlantic salmon DNA bar code standard detection gene, and primers and application thereof, and belongs to the field of molecular biology. The invention also provides a pair of primers for detecting the Atlantic salmon DNA bar code standard detection gene, and application of an Atlantic salmon DNA bar code standard detection sequence in identifying Atlantic salmons. The primers are used for amplifying Atlantic salmon DNA, an obtained PCR amplification product is subjected to nucleotide sequence determination, a species identification result is judged according to a sequence comparison analysis result, and if the genetic distance between the PCR amplification product and a nucleotide sequence shown in SEQ ID NO: 1 is 0.02 or below, it can be judged that a sample to be detected is Atlantic salmon. According to the method, the Atlantic salmons can be distinguished from other salmons and trout types.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Preparation method of oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed
InactiveCN103229742AAvoid dischargeRealize large-scale preparationClimate change adaptationPisciculture and aquariaDiseasePlant disease
The invention provides a preparation method of an oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed. The preparation method comprises the steps of: carrying out in vitro fertilization on oncorhynchus mykiss walbaum female parent fish ova and oncorhynchus mykiss male parent fish sperms, transferring into a 170-190 mg / L 6-DMAP (6-dimethylaminopyridine) solution with the temperature of 12-14 DEG C after fertilized ova is fertilized for 12-14minutes, soaking for 14-16minutes, then transferring in a water body with the temperature of 6-8 DEG C and then hatching; hatching the fertilized ovum to obtain hybrid triploid larva fishes after about 50 days; and detecting the larva fishes by using a flow cytometry, wherein the eyed rate of the fertilized ova in a hatching period is above 71 percent, the hatching rate of the seeds reaches above 90 percent and the triploid rate reaches above 80 percent. By using the preparation method of the oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed, the problems of sexual precocity, miniaturization, disease propagation and the like of individuals in a trout cultivating process are solved, the advantages of hybridization and triploid are obtained, and a good market value is achieved.
Owner:BEIJING FISHERIES RES INST
Method for offshore farming of large-size salmon and trout in temperate sea area
InactiveCN111838030ANo stress responseReduce mechanical damageClimate change adaptationPisciculture and aquariaOceanographyFish species
The invention discloses a method for offshore farming of large-size salmon and trout in temperate sea area. The method uses a sea-land relay method to cultivate fingerlings, the cultivated fingerlingsare put in offshore cages, low-temperature seawater resources in the lower layer of the sea area are used to ensure the stable farming temperature of the salmon and trout in the sea area, after the farming time exceeds one year, the large-size commodity salmon and trout are cultivated, the mechanical damage of salmon and trout is reduced, the mortality in farming is reduced, and the efficiency offarming is improved.
Owner:山东深海冷水团海洋开发有限公司
Preparation method of fish skin collagen peptide jelly capable of being stored and transported at normal temperature
PendingCN114041584AReduce pollutionIncrease added valueProtein composition from fishNatural extract food ingredientsBiotechnologyCollagenan
The invention discloses a preparation method of collagen peptide jelly capable of being stored and transported at a normal temperature. The collagen peptide jelly comprises the following raw materials: fish skin of salmon and trout, including one or more fish skin of Atlantic salmon, rainbow trout or steelhead trout, gelidium amansii as a natural coagulator, and condiments of lemon, white vinegar, cooking wine, scallion, ginger, garlic and the like. The fish skin collagen peptide is obtained by utilizing an enzymolysis extraction process and a hot water method secondary extraction process and adopting a series of technologies such as fishy smell removal, impurity removal and seasoning, and the collagen peptide jelly can be stored and transported at the normal temperature due to the addition of the gelidium amansii. The collagen peptide jelly prepared by the invention is a deep-processed product of various fish leftovers, is convenient to eat, makes up the vacancy that jelly and bean jelly products which can be stored and transported at normal temperature and take fish skin as a main raw material are not provided on the market temporarily, enriches the types of leisure foods capable of supplementing collagen, and has a wide development prospect.
Owner:OCEAN UNIV OF CHINA
Preparation method for steelhead trout and brook trout hybrid triploid seed
InactiveCN103238546AAvoid dischargeRealize large-scale productionClimate change adaptationPisciculture and aquariaDisease resistantFlow cytometry
The invention provides a preparation method for a steelhead trout and brook trout hybrid triploid seed. The preparation method includes the steps that a fertilized egg is obtained through hybridization of the steelhead trout and the brook trout; the fertilized egg is soaked in 6-DMAP (dimethylanminopyridine) solution, hatched and cultivated; and the hybrid triploid seed is produced. During incubation period, eyed rate of the fertilized egg is 77%, and hatching rate of the seed reaches above 90%. Then the obtained larvae is detected through a flow cytometry, and triploid rate of the obtained larvae reaches above 90%. With the preparation method, large-scale production of the steelhead trout and brook trout hybrid triploid seed is realized, and growth speed and disease-resistant ability of the trout are enhanced, thereby improving economic efficiency for farmers and bringing good market value.
Owner:BEIJING FISHERIES RES INST
Self-floating type fry incubator
PendingCN111587827AWill not affect water qualityEasy to cleanClimate change adaptationPisciculture and aquariaAnimal scienceEngineering
Owner:青海省渔业环境监测站
SNP molecular marker combination universal for salmon and bulltrout and application of combination
ActiveCN106282401AMaintain genetic diversityIncrease coverageMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceMendelian inheritance
The invention discloses an SNP molecular marker combination universal for salmon and bulltrout. The combination comprises 96 SNP molecular markers, and the base sequences of the SNP molecular markers are nucleotide sequences as shown in SEQ ID NO:1 to SEQ ID NO:96. The invention further discloses a method for evaluating genetic polymorphism of the salmon and bulltrout through the SNP molecular marker combination. The method includes the steps of firstly, obtaining genome DNA of the salmon and bulltrout needing to be specially evaluated; secondly, obtaining genotypes of a plurality of SNP molecular marker loci of each salmon or bulltrout; thirdly, analyzing the result of the genotypes of the SNP molecular marker loci of each salmon or bulltrout according to a Mendelian inheritance rule through bioinformatics software, conducting analyzing to obtain typing information of the salmon and bulltrout, judging the genetic relationship between the salmon and bulltrout, and determining whether the salmon and bulltrout are suitable as parents for propagation or not, wherein the SNP molecular markers are contained in the 96 SNP molecular markers. The invention further discloses a method for specially evaluating the salmon and bulltrout through the SNP molecular marker combination.
Owner:CHINESE ACAD OF FISHERY SCI
Salmon and trout roe hatching device
PendingCN113243321AAvoid dischargeTake advantage ofClimate change adaptationPisciculture and aquariaWater sourceWater resources
The invention discloses a salmon and trout roe hatching device, and relates to the technical field of hatching facilities. The salmon and trout roe hatching device comprises a plurality of hatching assemblies arranged in the vertical direction, and each hatching assembly comprises a water supply pipe group, a water collecting tank and a plurality of hatching barrels. In the same hatching assembly, each hatching barrel is placed in the water collecting tank, water in the hatching barrels can enter the water collecting tanks, and roe in the hatching barrels cannot enter the water collecting tanks; the water supply pipe groups are located above the hatching barrels and can supply water into the hatching barrels, the uppermost water supply pipe group is used for communicating with an external water source and the uppermost hatching barrel, the other water supply pipe groups can communicate with the water collecting tanks located above the water supply pipe groups and the hatching barrels located below the water supply pipe groups, and the lowermost water collecting tank can communicate with the outside. According to the salmon and trout roe hatching device, the utilization rate of water resources is increased, and the occupied area is saved.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Intelligent equipment for artificial insemination of salmon and trout and use method of intelligent equipment
ActiveCN113439690AEasy injectionMeet the needs of useClimate change adaptationPisciculture and aquariaAnimal scienceAI - Artificial insemination
The invention discloses intelligent equipment for artificial insemination of salmon and trout and a use method of the intelligent equipment. The intelligent equipment comprises a placement base, supporting legs, a fixing plate, a placement plate, a limiting plate, a nutrient solution adding device, a movable stirring device and a sperm adding device, wherein the surface of the bottom side of the placement base is fixedly connected to the top ends of the supporting legs, the top side of the left end of the placement base is fixedly connected to the fixing plate, the placement plate is placed on the surface of the top side of the placement base, and the nutrient solution adding device comprises a fixing column, an adding pipe, a valve and an adding nozzle. According to the intelligent equipment for the artificial insemination of the salmon and trout and the using method of the intelligent equipment, the design is novel, the structure is simple, placement trays can be placed in order conveniently, nutrient solutions can be added conveniently, the contact opportunities of sperms and eggs of the salmon and trout are effectively increased, the success rate of insemination and breeding efficiency are improved, meanwhile, the manual operation requirement and labor intensity are greatly reduced through intelligent control, time and labor are saved, and the intelligent equipment for the artificial insemination of the salmon and trout and the using method of the intelligent equipment are easy to popularize and apply in large-scale artificial breeding of the salmon and trout.
Owner:山东康科润海洋科技有限公司 +2
Salmon trout ihnv monoclonal antibody and its detection kit and application
ActiveCN111560353BStrong specificityAvoid cross reactionSsRNA viruses negative-senseBiological material analysisNecrovirusJuvenile fish
The invention provides a salmon trout IHNV monoclonal antibody, which is obtained by secreting a monoclonal hybridoma cell line specific to IHNV N protein produced by immunizing animals with the infectious hematopoietic organ necrosis virus IHNV-BJLL strain. The invention also provides a detection kit and application based on the monoclonal antibody. Among the IHNV monoclonal antibodies obtained in the present invention, the monoclonal antibody secreted by a hybridoma cell line has strong specificity, can detect the infection of domestic U-type and J-type salmon trout IHNV at the same time, and can avoid the occurrence of other rhabdoviruses The cross-reaction overcomes the misdetection and missed detection of IHNV-infected juvenile fish by current molecular biology. The monoclonal antibody secreted by another strain is more specific and can detect U-type salmon trout IHNV infection, but it has no reaction with J-type salmon trout IHNV, so it can be used to identify the genotype of salmon trout IHNV in China. The detection method of the invention can directly observe the result through a fluorescence microscope, and this operation can be completed in a common laboratory, and has a good application prospect.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
A kind of intelligent equipment for artificial insemination of salmon and trout and its application method
ActiveCN113439690BEasy injectionMeet the needs of useClimate change adaptationPisciculture and aquariaAnimal scienceMedicine
This application discloses an intelligent equipment for artificial insemination of salmon and trout and its use method, including a placement seat, support legs, a fixed plate, a placement plate, a limit plate, a nutrient solution adding device, a mobile stirring device and a sperm adding device; The bottom side surface of the placement seat is fixedly connected to the top of the support leg, the top side of the left end of the placement seat is fixedly connected with the fixed plate, the top side surface of the placement seat is placed with the placement plate, and the nutrient solution is added The device includes a fixed column, an adding tube, a valve and an adding nozzle. The intelligent equipment for artificial insemination of salmon and trout and its use method are novel in design and simple in structure, which is convenient for orderly placing the placement tray and adding nutrient solution, effectively increasing the chance of contacting sperm and eggs of salmon and trout, thereby improving the chance of insemination Success rate and breeding efficiency, while intelligent control greatly reduces the need for manual operation and labor intensity, saves time and effort, and is easy to promote and apply in large-scale artificial breeding of salmon and trout.
Owner:山东康科润海洋科技有限公司 +2
Preparation method of game fish triploid fries
InactiveCN102696515BHigh speedFast growthClimate change adaptationPisciculture and aquariaGame fishHeat shock
The invention provides a preparation method of game fish triploid fries. The preparation method comprises the following steps of: hybridizing a rainbow trout female parent fish and an arctic char male parent fish; transferring fertilized ova which have been fertilized for 20 minutes under the condition of 7 DEG C into a water body of 26 DEG C and performing heat shock for 20 minutes; then transferring into a water body of 7 DEG C to hatch for about 60 days; and hatching fish fries from the fertilized ova, wherein the eyed rate of the fertilized ova in the hatching period is over 75 percent, the hatching rate of the fries reaches over 90 percent, and the triploid rate of the obtained fries is 90-95 percent through detection of a flow cytometer. According to the preparation method of game fish triploid fries provided by the invention, the problems of sexual precosity, size miniaturization, disease breeding and the like of bred individuals due to long-term close breeding and shortage of systematic breeding in the rainbow trout culturing process are relieved, meanwhile, the hybrid triploid has the two advantages of the hybridization and the triploid, the growth speed and the disease resistance are obviously superior to those of the conventional diploid rainbow trout, and the preparation method has higher economic benefit and market value.
Owner:BEIJING FISHERIES RES INST
A method for constructing a new strain of salmon trout resistant to saprolegniasis
InactiveCN106912408BAvoid damageGood genetic stability of disease resistance traitsClimate change adaptationPisciculture and aquariaAntibiosisDisinfectant
The invention discloses a new water mildew-resistant salmon trout construction method, and relates to a new disease-resistant salmon trout construction method. The method can be used to reduce salmon trout loss caused by water mildew without using a lot of disinfectant or antibiosis medicament, thereby raising salmon trout quality. The method comprises the steps of 1 establishing a basic seed selection group; 2 establishing family systems in the number of N; 3 weeding out; 4 secondary water mildew infection; 5 breeding to establish filial generation family systems in the number of M; and 6 repeating steps 1 to 5 until water mildew-resistant property is stable. According to the invention, beneficial colony heredity variation is utilized, and new disease-resistant species or lines are cultivated through fertile breeding; the new species has good heredity of disease resistance is stable; the appearance of the fish as a goods is prevented from being damaged due to water mildew; and mortality caused by water mildew is reduced to 30% below.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
A method to improve the utilization rate of rainbow trout pseudo-male sperm
The invention discloses a method for increasing the utilization rate of sperms of sex reversal milter of rainbow trout, relates to a method for increasing the utilization rate of sperms of milter, and aims to solve the problems that the sperm activation rate, the fish egg fertility rate and the eyed rate are low after sperms of sex reversal milter of rainbow trout are treated by using a conventional method. The method comprises the following steps: I, preparing a rainbow trout sex reversal milter semen storage liquid; II, after the semen storage liquid is prepared, adjusting the pH value, and leaving to stand at 8-12 DEG C for later use; III, cutting high-quality and healthy rainbow trout so as to obtain a sex reversal milter spermary, putting the sex reversal milter spermary into a clean mortar without water, grinding the sex reversal milter spermary so as to obtain a mixed semen tissue liquid, filtering the mixed semen tissue liquid by using gauze, mixing the sex reversal milter semen with the semen storage liquid, putting into an oxygen bag, storing, and taking out for fish egg fertilization. The activation rate of the sperms of the sex reversal milter can reach more than 90%, and the fertilization rate and the eyed rate of fish eggs are both more than 70%, therefore, waste of the fish eggs can be greatly reduced. The method is applicable to the field of genetic breeding of the rainbow trout.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Method for preparing hybrid triploid seeds of golden trout and arctic charr
InactiveCN103283649AAvoid dischargeRealize large-scale preparationClimate change adaptationPisciculture and aquariaDiseaseOospore
The invention provides a method for preparing hybrid triploid seeds of golden trout and arctic charr. The method includes: subjecting ova of female parent golden trout and sperms of male parent arctic charr to external fertilization, moving fertilized ova to a six-DMAP (N6-dimethyladenine) solution with concentration ranging from 170mg / L to 190mg / L and the temperature ranging from 9 DEG C to 11 DEG C after the fertilized ova are fertilized for 12-14 minutes, soaking for 14-16 minutes prior to moving into water at the temperature of 6-8 DEG C for hatching, and obtaining the hatched hybrid triploid larva fishes from the fertilized ova after about 50 days. During hatching, the eyeing rate of the fertilized ova is larger than 78%, the seed hatching rate reaches more than 90%, the obtained larva fishes are detected by a flow cytometer, and the triploid rate reaches 95%. The method for preparing the hybrid triploid seeds of the golden trout and the arctic charr remits the problems about sexual precocity, size miniaturization, disease breeding and the like of individuals during salmon and trout cultivation, and has the advantages of hybridization and triploid and a good marketing value.
Owner:BEIJING FISHERIES RES INST
Production method of Oncorhynchus Mykiss triploid fry
InactiveCN103283650BAvoid dischargeRealize large-scale preparationClimate change adaptationPisciculture and aquariaDiseasePrecocious puberty
Owner:BEIJING FISHERIES RES INST
A three-dimensional polyculture method and production system for salmon, trout, flounder and flounder
ActiveCN106342729BIncrease profitAvoid wastingSeawater treatmentClimate change adaptationPolycultureWater resources
The invention relates to the technical field of for cultivating salmon and trout and provides a solid mixed cultivation method for salmon and trout flatfish. The salmon and trout and the flounder are cultivated in a same cultivation pool together. The use ratio of the cultivation water can be greatly improved by the complementary feature of life water of salmon and trout and the flounder, and then the unit water yield and effect are improved; and the maximum economic benefit is realized by using the limited water resource.
Owner:国信东方(烟台)循环水养殖科技有限公司
Reagent for multiple detection of salmon trout virus and its application
ActiveCN110387440BShorten detection timeQuick checkMicrobiological testing/measurementDNA/RNA fragmentationViral nucleic acidSalmonidae
The invention discloses a reagent for multiple detection of salmonidae virus and an application thereof. The reagent provided by the invention comprises a specific primer group, wherein the specific primer group comprises at least one pair from a first primer pair to a sixth primer pair; the first primer pair to the sixth primer pair are sequentially primer pairs of: nucleic acid of specifically-amplified spring viraemia of carp virus, nucleic acid of infectious hematopoietic necrosis virus, nucleic acid of viral haemorrhagic septicaemia virus, nucleic acid of infectious salmon anaemia virus,nucleic acid of salmonid alphavirus, and nucleic acid of infectious pancreatic necrosis virus. The upstream and downstream primers of the first primer pair to the sixth primer pair are sequentially the sequences as shown in Seq ID No.1 to Seq ID No.12. The reagent can carry out multiple detection on six salmonidae viruses, is simple and convenient to use, shortens the detection time of the six salmonidae viruses, improves the detection efficiency and quality, and has great significance for rapid detection and prevention and control of the six salmonidae viruses.
Owner:SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT
Feeding methods for salmon and trout farming
ActiveCN107950442BIncrease profitImprove farming efficiencyClimate change adaptationPisciculture and aquariaFish weightAmmoniacal nitrogen
The invention discloses a salmon trout cultivating and feeding method, and belongs to the technical field of aquaculture. The technical point of the invention is that the salmon trout cultivation is performed in two sets of sealed recirculating aquaculture systems, and every set comprises 24 culture ponds, and water for cultivation is well water; the cultivation index is that the temperature of water for cultivation is 15-17 DEG C, the dissolving oxygen is 7-8 / mg / L, and water quality indexes of ammonia nitrogen, nitrate, and nitrite are the same condition; the food feeding parameters: the daily feeding amount includes four groups which are 0.7%, 1%, 1.5% and 1.8% of fish weight; the daily feeding frequency is 4 times, and the feeding time is set at 6: 00, 12: 00, 18: 00 and 24: 00; the overall growth condition of the fish is tested once monthly; the survival rate and fatness of fish are calculated; water quality is monitored at fixed time and regular period; the detection position is awater returning pond. The invention is used for salmon trout cultivation.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Biosensing detection method for salmon trout seed identification
InactiveCN107058625BShort reaction timeQuick checkMicrobiological testing/measurementMicroorganism based processesTotal rnaPlant Sources
The invention discloses a biosensing detection method for identification of trout fries. The biosensing detection method is characterized in that on the basis of conservative analysis results of IHNV (infectious hematopoietic necrosis virus) gene sequence from different plant sources in a public database, six specific primers using IHNV virus matrix protein (M) encoding genes as amplification targets are designed, the constant-temperature reverse transcription amplification is performed on the to-be-measured total RNA template of the fish egg or fry sample, and the amplification time is only 15 to 30min; all or part of primers have the electrochemical activity markers in the amplification reaction, one part of dNTP has an anchor marker, the successfully amplified virus M gene sequence simultaneously has the electrochemical activity marker and the anchor marker, the amplified product can be captured onto the electrode surface by the anchor marker, and the carrying of IHNV in the sample can be accurately judged through detecting the electrochemical signal at the surface of the electrode. The biosensing detection method has the advantages that the operation is simple, the reaction is quick, the requirement on equipment precision is low, and the method is suitable for the site quick detection of production and business workplaces of fry farms, culture farms, and the like.
Owner:CHINESE ACAD OF FISHERY SCI
Preparation method for steelhead trout and brook trout hybrid triploid seed
InactiveCN103238546BAvoid dischargeRealize large-scale productionClimate change adaptationPisciculture and aquariaDisease resistantFlow cytometry
The invention provides a preparation method for a steelhead trout and brook trout hybrid triploid seed. The preparation method includes the steps that a fertilized egg is obtained through hybridization of the steelhead trout and the brook trout; the fertilized egg is soaked in 6-DMAP (dimethylanminopyridine) solution, hatched and cultivated; and the hybrid triploid seed is produced. During incubation period, eyed rate of the fertilized egg is 77%, and hatching rate of the seed reaches above 90%. Then the obtained larvae is detected through a flow cytometry, and triploid rate of the obtained larvae reaches above 90%. With the preparation method, large-scale production of the steelhead trout and brook trout hybrid triploid seed is realized, and growth speed and disease-resistant ability of the trout are enhanced, thereby improving economic efficiency for farmers and bringing good market value.
Owner:BEIJING FISHERIES RES INST
Preparation method of oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed
InactiveCN103229742BAvoid dischargeRealize large-scale preparationClimate change adaptationPisciculture and aquariaDiseasePlant disease
The invention provides a preparation method of an oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed. The preparation method comprises the steps of: carrying out in vitro fertilization on oncorhynchus mykiss walbaum female parent fish ova and oncorhynchus mykiss male parent fish sperms, transferring into a 170-190 mg / L 6-DMAP (6-dimethylaminopyridine) solution with the temperature of 12-14 DEG C after fertilized ova is fertilized for 12-14minutes, soaking for 14-16minutes, then transferring in a water body with the temperature of 6-8 DEG C and then hatching; hatching the fertilized ovum to obtain hybrid triploid larva fishes after about 50 days; and detecting the larva fishes by using a flow cytometry, wherein the eyed rate of the fertilized ova in a hatching period is above 71 percent, the hatching rate of the seeds reaches above 90 percent and the triploid rate reaches above 80 percent. By using the preparation method of the oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed, the problems of sexual precocity, miniaturization, disease propagation and the like of individuals in a trout cultivating process are solved, the advantages of hybridization and triploid are obtained, and a good market value is achieved.
Owner:BEIJING FISHERIES RES INST
Preparation method for steelhead trout and arctic char hybrid triploid seed
InactiveCN103238544BAvoid dischargeRealize large-scale productionClimate change adaptationPisciculture and aquariaDiseasePrecocious puberty
The invention provides a preparation method for a steelhead trout and arctic char hybrid triploid seed. The preparation method includes the steps that steelhead trout female broodstock egg and arctic char male broodstock sperm are in vitro fertilized; after the fertilized egg is fertilized for 12-14 minutes, the fertilized egg is moved into 6-DMAP (dimethylanminopyridine) solution with concentration of 170-190 milligrams per liter and temperature of 9-11 degrees Celsius to be soaked for 14-16 minutes; and then the soaked fertilized egg is moved into water of the temperature of 6-8 degrees Celsius to be hatched. After about 50 days, a hybrid triploid larvae is hatched by the fertilized egg. During incubation period, eyed rate of the fertilized egg is above 81%, and hatching rate of the seed reaches above 90%. Then the obtained larvae is detected through a flow cytometry, and triploid rate of the obtained larvae reaches 94%. With the preparation method for the steelhead trout and the arctic char hybrid triploid seed, during trout breeding process, problems of precocious puberty, individual miniaturization, disease breeding and the like in an individual fish are alleviated. Furthermore, the preparation method has the advantages of hybrid and triploid and has good market value.
Owner:BEIJING FISHERIES RES INST
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