159 results about "Verticillium species" patented technology

The invention discloses Bacillus subtilis, which is named Bacillus subtilis. The Bacillus subtilis was collected in China General Microbiological Culture Collection Center on January 15th, 2009, with a collection number of CGMCC No.2843. The Bacillus subtilis strain disclosed by the invention is screened from tobacco leaf habitats. Experiments prove that the fermentation liquor of the strain has a strong inhibition effect on fungal pathogens such as Alternaria alternate and pepper anthracnose and demonstrates high prevention effect on Alternaria alternate, tobacco black shank, pepper anthracnose, tomato wilt, phytophthora melonis Katsura, verticillium dahlia and the like and has high growth promoting effects on tobacco, pepper, cucumber, tomato and eggplant. Therefore, the Bacillus subtilis disclosed by the invention can be used for preparing biological bactericides for preventing and controlling fungi in tobacco and vegetables and can be used for preparing growth promoting agents for promoting the growth of tobacco or vegetables.

Lipolytic enzymes with high activity at alkaline pH in the absence of Ca++ can be obtained from filamentous fungi of the genera Gliocladium, Verticillium and Trichophaea and that the lipolytic enzymes are effective for improving the effect of detergents. The lipolytic enzymes have a good washing performance, as expressed by the hydrolysis of oil on textile swatches. The amino acid sequences of the lipolytic enzymes are highly homologous.

The invention relates to a pesticide composition for relieving the phytotoxicity of herbicide. Effective components are a plant growth regulating agent and active polysaccharide, wherein the plant growth regulating agent is any one or a mixed agent of two of compound nitrophenolate and diethylaminoethyl hexanoate; the active polysaccharide is any one or a mixed agent of two of chitosan oligosaccharide and poly-glucosene; and the pesticide composition comprises the effective components in parts by weight of 0.05-90.0 parts of the plant growth regulating agent and 0.05-90.0 parts of the active polysaccharide. The pesticide composition comprehensively utilizes the synergism effect of the plant growth regulating agent and the active polysaccharide to resist disease source force and relieve the phytotoxicity of the herbicide. The pesticide composition is further applicable to crop exogenous damages including hormone phytotoxicity, low-temperature frozen injury, salt alkaline injury and the like, and also can be used for crop diseases of crops virus diseases, verticillium wilts and the like.

The present invention relates to an application of anthraquinone derivative as pesticice for controlling plant fungous diseases. The anthraquinone derivative pesticide contains one kind or several kinds of 10 anthraquinone derivative compounds. The anthraquinone derivative compounds have obvious action for preventing and curing several plant diseases, and can be used for preventing and curing economic crop, grain crop, vegetable, fruit tree and melon diseases due to fungi, including sphaerotheca, erysiphe, blumeria, fusarium, sclerotinia, pyricularia, rhizoctonia, colletotrichum, botrytis, verticillium and alternaria.

The invention provides a functional bactericide for preventing and treating root-knot nematode.The functional bactericide comprises an azotobacter bactericide, a biocontrol bacterium bactericide and a carrier, and the mass ratio of the azotobacter bactericide to the biocontrol bacterium bactericide to the carrier is (1-2.5):(1.5-5):1.The azotobacter bacterium bactericide contains nodule bacteria and azotobacter chroococcum, and the living bacterium count ratio of nodule bacteria to azotobacter chroococcum is (1-100):(1-200).The biocontrol bacterium bactericide contains paecilomyces lilacinus, verticillium chamydosporium, bacillus subtilis and bacillus cereus, and the living bacterium count ratio of paecilomyces lilacinus to verticillium chamydosporium to bacillus subtilis to bacillus cereus is (1-100):(1-100):(1-100):(1-100).The living bacteria count of the functional bactericide is larger than 0.2 billion / g.When applied to fertilizer, the functional bactericide can improve the disease preventing effect and the growth promoting effect of microbial fertilizer, guarantee nutrition supply of microbial fertilizer in the applying process, achieve the effects of preventing and treating root-knot nematode, forming healthy root nodules and promoting plant growth, increase the yield of crops and improve the quality of crops.

The invention discloses broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of the broad-spectrum disease resisting, growth promotion and stress resisting bacillus. The broad-spectrum disease resisting, growth promotion and stress resisting bacillus is bacillus sp. WXCDD105, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is 3, No.1 Yard, Western Beichen Road, Chaoyang District, Beijing, the preservation date is May 25, 2016, and the preservation number is CGMCC No.12496. A biocontrol strain provided by the invention has ultraviolet-resisting and drought-resisting properties, has stronger prevention and treatment effects on the tomato gray mold and the tomato leaf mold, and can inhibit fungal diseases including wilt fusarium of a plurality of types of crops, corn foot root bacteria, rice wilt pathogens, acanthopanax senticosus root rot pathogens, corynespora cassiicolai, colletrichum orbiculare, exserohilum turcicum, tomato spot blight fungi, sunflower sclerotinia sclerotiorum, botrytis cinerea, fusarium oxysporum, verticillium fusarium, alternaria alternata and the like; the broad-spectrum disease resisting, growth promotion and stress resisting bacillus has a growth promotion effect on tomato seeds and seedlings and can also improve the quality of fruits and reduce the rotting rate.

The invention discloses Bacillus subtilis strain HMB26553 (Bacillussubtilis), the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCCNo.13211. The present invention also discloses a microbial agent produced by use of the strain and application thereof in the prevention and control of plant diseases such as cotton damping-off and the like. The Bacillus subtilis HMB26553 has high control efficiency on the cotton damping-off, the average control efficiency is above 90%; the microbial agent has good effect perdurability, and has no environmental pollution problems; in addition, the HMB26553 has a wide antibacterial spectrum, and has a good inhibitory effect on cotton rhizoctonia solani kuh, cotton verticillium dahlia, cotton Fusarium oxysporum, tomato botrytis cinerea and cucumber Corynespora cassiicola.

The invention discloses an application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt. The Bacillus methylotrophicus ZBL-1 with CGMCC No.11636 and higher inhibition effect on verticillium dahlia is obtained through separation, screening, breeding and domestication of soil samples collected in the cotton fields with severe diseases in Shihezi city and is obviously different from common Bacillus methylotrophicus strains, and meanwhile, the control effect of the screened strain ZBL-1 on the cotton verticillium wilt in indoor pot culture experiments is 73.0%; the control effect of the screened strain ZBL-1 in field plot experiments is 43.1%, and whether in development of a new biopesticide or biological control agent, the Bacillus methylotrophicus ZBL-1 as a biological control material for the cotton verticillium wilt has very good application prospect and has realistic significance and functions.

The invention discloses a panax notoginseng antimicrobial peptide gene PnSN1. The nucleotide sequence of the PnSN1 gene is shown as SEQ ID NO:1, and the PnSN1 gene is used for coding snakin antimicrobial peptide. The functional genomics correlation technique study proves that the PnSN1 gene has the function of improving the plant fungal infection resistance capability; the antifungal PnSN1 gene is built onto a plant expression vector and is transferred into tobaccos for overexpression; the transgenosis tobacco has high in vitro antifungal activity. The transgenosis tobacco subjected to PnSN1 overexpression has the strong inhibiting effect on the growth of fusarium oxysporum, fusarium solani, verticillium fusarium, botryosphaeria dothidea and beaded gibberella.

The invention discloses a verticillium dahlia delta VdKu70 defective mutant strain and application thereof. The verticillium dahlia delta VdKu70 defective mutant strain is characterized in that a gene knock-out vector carrying a Ku70 gene homologous recombination DNA fragment is constructed, then the agrobacterium-mediated verticillium dahlia is subjected to genetic transformation, and a Ku70 gene-deleted VdKu70 defective mutant strain can be screened from mass transformants; the microbe preservation number of the Ku70 gene-deleted VdKu70 defective mutant strain is CGMCC No.10107. The obtained verticillium dahlia delta VdKu70 defective mutant strain has the morphological characteristics and virulence which are basically the same as those of a wild strain and is more sensitive to UV treatment; with the adoption of the verticillium dahlia delta VdKu70 defective mutant strain for gene targeting as a background strain, the targeting efficiency is increased by 40 to 60% compared with the wild strain. The background strain is provided for the study on verticillium dahlia infection mechanisms, coexistence mechanism with a host, and key pathogenic target gene.

A method of improving the growth of plants belonging to the family Cannabaceae sensu stricto by providing a plant nutrient solution about the plant roots and a gas mixture circulating about the plant shoot; by selecting the gas mixture and plant nutrient solution temperature independently of the other; and providing a plant nutrient solution to gas mixture temperature differential of approximately 0° F. or of at least approximately 15° F. during different phases of plant development, in order to change the plant development to improve a desired plant organ for industrial, scientific, and medical purposes. Infection of plants by Pythium, Fusarium, Verticillium, and Rizoctonia are treated or prevented by selecting a gas mixture temperature that is approximately 65 to 85° F. and a plant nutrient solution temperature that is approximately 35 to 65° F.

The invention discloses a verticillium dahlia secreting type protein elicitor VdPEL1 and application thereof. The protein can improve the plant disease resistance and can be used as an inductor for inducing the plant immunity and can be applied to the field of plant disease prevention and treatment. The amino acid sequence of the protein is shown as SEQ ID NO:2. The engineering strain expression is used for obtaining high small-to-big protein, so that the disease resistance of tobaccos and cotton can be improved; the related plant diseases include tobacco mosaic, gray mold and cotton verticillium wilt. The protein elicitor can obviously improve the plant disease resistance; the effect is fast; the action time is long. The VdPEL1 provides a new path for improving the plant disease resistance.

The present invention relates to methods for identifying sunflower lines having increased resistance to Verticillium wilt, and identification of genetic markers linked to gene(s) conditioning such increased disease resistance. The present invention also relates to methods of breeding sunflower plants from lines having increased Verticillium wilt resistance by marker-assisted selection, compositions including nucleic acid probes or primers which are useful for such marker assisted selection, and plants and plant parts produced by such methods.

The invention discloses paenibacillus polymyxa for preventing and treating vertieillium wilt in crops and application of the paenibacillus polymyxa. The strain is named as paenibacillus polymyxa SX-3, with preservation number as CGMCC 11638. The invention provides an inoculant containing the paenibacillus polymyxa. The strain of the invention has an inhibitory effect on both spore germination and mycelial growth of verticillium dahlia and the strain has an efficient antagonistic action. The strain of the invention is capable of preventing and treating crop verticillium wilt caused by the verticillium dahlia, the strain has a significant effect on preventing and treating cotton verticillium wilt and control efficacy of pot experiments reaches 86% or above; and bacterium liquid containing the strain of the invention, when irrigated to the roots of crops in a late disease period, can still achieve a significant therapeutic effect; therefore, the strain has a great potential in the application to bio-pesticides.

The invention relates to a complex inoculant preventing and treating verticillium dahliea wilt on a tomato, and a preparation method and an application of the complex inoculant. The complex inoculant is formed by mixing a fermentation product of bacillus amyloliquefaciens MES812, a fermentation product of bacillus subtilis MES810 and a fermentation product of bacillus mucilaginosus MES803 at a volume ratio of 1:(1-3):(1-5). The preparation method of a strain in the complex inoculant comprises the following steps of strain activation, primary seed solution preparation, secondary seed solution preparation, fermentation tank fermentation and discharge to form the fermentation product. Major microorganisms in the complex inoculant are reproduced extensively in soil to antagonize growth of pathogenic bacteria; harmful microorganisms in the soil are effectively inhibited or killed; a powerful environment is provided for reproduction and growth of beneficial microorganisms; and the occurrence of the verticillium dahliea wilt on the tomato is prevented and controlled effectively by continuous formation of metabolic products of the beneficial microorganisms. At the same time, the soil is improved, the soil fertility is improved, and the output and quality of the tomato are improved.

The invention relates to the field of biologically-derived insecticidal preparations, in particular to a complex microbial inoculant for controlling root-knot nematodes of crops and a preparation method of the complex microbial inoculant. The complex microbial inoculant for controlling the root-knot nematodes of the crops and the preparation method of the complex microbial inoculant are characterized in that microorganism is composed of bacillus subtilis, bacillus sphaericus, pseudomonas fluorescens, bacillus thuringiensis, brevibacillus laterosporus, paenibacillus polymyxa, pasteurella pneumoniae, paecilomyces lilacinus, verticillium chalamydosporium, beauveria bassiana and aspergillus fumigatus. The novel multi-bacteria same-pot fermentation technology is adopted, the formula is clever and reasonable, the disadvantages of mutual antagonism in the liquid deep fermentation culture of compound bacteria are eliminated, the compound bacteria has high activity, and the complex microbial inoculant for controlling the root-knot nematodes of the crops has a good control effect on tomatoes and cucumber root-knot nematodes.

The invention relates to a use of VdP4-ATPase gene for coding Verticillium dahlia P type ATPase in improvement of the verticillium wilt resistance of plants, and a method for improving the verticillium wilt resistance of the plants. The VdP4-ATPase gene for coding the erticillium dahlia P type ATPase is expressed in target plants to improve the verticillium wilt resistance of the plants. Detoxification of pathogen toxins is used to improve the resistance of the plants, and the pathogen race resistance characteristic does not exist. The method can be widely applied in improvement of the resistance of different pathogen physiologic races without the race-specific resistance characteristic.

A process for greatly producing chlamydospores by liquid fermentation of verticillium chlamydosporium features that on the basis of previous technique, the solid bacterial strain instead of liquid one is used, and the depressant composed of thiophanate-methyl, carbendazim and sodium silicoalginate is added to create the condition benefiting growth of chlamydospores.

The invention relates to phytopathology, and particularly relates to endophytic fungi CEF-193 of cotton and application thereof. The bacterial strain CEF-193 (Acremonium alternatum) with a significant induced immune function on cotton verticillium wilt is obtained from the endophytic fungi of the cotton by separation, screening, identifying and biological assay of a greenhouse, the preservation number is CGMCC No.8302, and CEF-193 can be cultivated on an artificial medium, and is easily obtained and cultivated. Thus, industrialized production and popularization and application are facilitated. An indoor bacteriostatic test shows that the control effect on greensickness is good, verticillium dahlia is inoculated 4 days after the CEF-193 is firstly inoculated, the prevention and control effect on the cotton verticillium wilt achieves 74.1%, and the endophytic fungi CEF-193 have significant control action on the greensickness. Compared with the bacillus subtilis mainly applied to production, the prevention and control effect is improved by about 40%, and the endophytic fungi CEF-193 are safe on people and livestock, insects and crops, and friendly to environment.

The invention discloses a pathogen verticillium antagonistic bacterium and application thereof, and belongs to the technical field of biological control over crop diseases. Bacillus pumilus Y106 is adopted as the pathogen verticillium antagonistic bacterium, the preservation number is CCTCC NO:M2014423, the preservation date is September 17, 2014, the preservation place is China Center for Type Culture Collection (CCTCC), and the preservation address is Wuhan University in Wuhan China. The bacterial strain is Gram-positive, mesophilic and aerobiotic and can resist the high temperature of 65 DEG C; the bacterial strain comprises the main morphological characteristics that the bacterial strain is shaped like a thin rod, the two ends are bluntly round, and the bacterial strain mostly exists in a single mode or in a chain-arranged mode. The pathogen verticillium antagonistic bacterium has the antagonistic effect on pathogen verticillium-verticillium dahliae, can inhibit germination of pathogen spores and can inhibit paroxysm of cotton verticillium wilt to a certain degree.

The invention discloses a novel actinomycin strain which generates actinomycin D: Streptomycesmutabilis TRM45540, preserved in U. S. Department of Agriculture Agricultural Research Service (ABS) Culture Collection on 14th, April, 2014 with the preservation number of NRRL B-59117. Meanwhile, the invention further discloses a seed medium which efficiently produces actinomycin D by using the strain, a fermentation medium and a preparation process of extracting and separating actinomycin D from fermentation liquor. An antibacterial activity test shows that the strain and the actinomycin D generated by fermentation of the strain have broad-spectrum and efficient antagonistic activity on pathogenic bacteria such as cotton verticillium and fusarium wilts, walnut pythium ultimum, staphylococcus aureus, staphylococcus epidermidis, candida albicans and the like and have an important application value and a very good application and development prospect on safe production of crops such as cotton, walnuts and the like and in preventing and controlling diseases such as bovine mastitis, women's vaginitis and the like.

The present invention discloses an inhibiting and killing plant pathogenic fungi active constituent of curcuma longa and its preparation and application. The active component is extract extracted by ethanol or methanol from curcuma longa or fine extract extracted further by n-hexane based on the extract extracted by ethanol or methanol. The inhibiting and killing plant pathogenic fungi active constituent of curcuma longa can be prepared inhibiting and killing plant pathogenic fungi and mushroom pathogenic fungi preparation, especially prepared preparation for inhibiting and killing phorna wasabiae Yokogi, sclerotinia sclerotiorum, tomato botrytis cinerea, verticillium, wheat phytoalexin, edible fungi mycogone perniciosa, olive green mycophenolate or P. pallidum. The preparation formulations can be water solution, soluble powder, wettable powder, thick suspending agent and granule. The active components disclosed by this present invention has extensive source of raw materials, no-toxic, easy degradation features, can be developed a non-pollution botanical inhibiting and killing fungi preparation.

The invention discloses a real-time fluorescent quantitation PCR (Polymerase Chain Reaction) method for detecting Chinese cabbage verticillium pathogenic bacteria. A pair of primers disclosed by the invention consists of a DNA (Desoxvribose Nucleic Acid) molecule shown as SEQ ID No.1 and a DNA molecule shown as SEQ ID No.2. The method disclosed by the invention is a rapid, accurate and efficient verticillium-resistant molecular diagnosis system, and a good foundation is laid for the screening and resistant transformation of Chinese cabbage verticillium resources. Moreover, the method can be used for predicting and forecasting Chinese cabbage verticillium, and has important significance to the safe production of Chinese cabbage.

The invention discloses a medium for improving the antibacterial activity of biocontrol bacillus to plant pathogenic fungi, comprising the following components: potato, sucrose, maize paste, sodium L-glutamate, phosphate, MgSO4.7H2O, CaCl2, ferric citrate, MnSO4.H2O, phytic acid, L-proline, threonine, tetramethyl glutaric aciduria, gamma-aminobutyric acid, sodium nitroprusside, hydrocortisone, LaCl3, amino-oligosaccharin, chamomile leach liquor, and celery juice. A biocontrol bacillus seed solution is inoculated into the medium for temperature-variable fermentation culture, so as to promote the growth and reproduction of bacillus, and effectively stimulate the activity of bacillus, enhance the secretion of bacteriostatic substances and the activity of pathogen hydrolase, thereby significantly improving the antibacterial activity of bacillus to plant pathogenic fungi such as fusarium oxysporum, verticillium dahlia, botrytis cinerea, sclerotinia sclerotiorum, and providing a theoreticalbasis for the further development and application of biocontrol bacillus.

The present invention relates to the field of plant diseases, particularly to applications of verticillium dahlia pathogenicity related gene VdPR1 as the anti-verticillium dahlia target gene. According to the present invention, the colony morphology, the growth rate, the extracellular enzyme activity and the pathogenicity of the knockout mutant [delta]VdPR1 and the complementary mutant [delta]VdPR1-C are determined, and are subjected to compared analysis with various indexes of the wild type, such that it is determined that the VdPR1 gene is the verticillium dahlia pathogenicity related gene, is associated with microsclerotium production and melanin accumulation, and provides promotion effects for activities of cellulase and protease.

The invention discloses pseudomonas aeruginosa for preventing and treating vertieillium wilt in crops and application of the pseudomonas aeruginosa. The strain is named as pseudomonas aeruginosa KK9a, with preservation number as CGMCC 11637. The invention provides an inoculant containing the pseudomonas aeruginosa. The strain of the invention has an inhibitory effect on both spore germination and mycelial growth of verticillium dahlia and the strain has an efficient antagonistic action. The strain of the invention is capable of preventing and treating crop verticillium wilt caused by the verticillium dahlia, the strain has a significant effect on preventing and treating cotton verticillium wilt and control efficacy of pot experiments reaches 86% or above; therefore, the strain has a great potential in the application to bio-pesticides.

The invention discloses a pseudo-ginseng Dirigent-like protein gene PnDIR1 and application thereof. The research by correlation techniques of molecular biology and functional genomics proves that the PnDIR1 gene has the antifungal disease function of plants. When the antifungal gene PnDIR1 is constructed onto a plant expression vector and transformed into the tobacco to be overexpressed, the transgenic tobacco plant has very high in-vitro antifungal activity; and the PnDIR1-overexpressed transgenic tobacco has obvious inhibiting actions on growth of Botryosphaeria dothidea, Fusarium solani and Verticillium Fusarium.

The invention discloses a Bacillus subtilis strain WKPHY-54, collected under CGMCC No. 14951, and also discloses a microbial agent containing the strain. The strain WKPHY-54 can degrade organophosphorus and prevent diseases, utilization rate of phosphate fertilizer or organophosphorus in soil can be increased, crop growth can be promoted, fertilizer input can be decreased, and production cost canbe decreased; in addition, the strain WKPHY-54 can effectively prevent plant diseases such as eggplant verticillium wilt, averagely up to 70.0% and above; further, the strain WKPHY-54 has a wide antibacterial spectrum, and has good inhibitory effect for eggplant verticillium, cucumber verticillium, Fusarium vasinfectum, potato Phyllachora and the like; more additionally, the strain WKPHY-5 is highly specific to the plant diseases such as eggplant verticillium wilt, rarely causes resistance and has long-lasting effect.

The invention discloses anti-verticillium wilt protein from sea island cotton as well as a coding gene and application thereof. The protein provided by the invention is following (a) or (b): (a), protein consisting of an amino acid sequence as shown by sequence 1 in a sequence table; (b), protein, obtained by substituting and / or deleting and / or adding with one or more amino acid residues of the amino acid sequence of the protein limited by (a), and associated with plant verticillium wilt disease. Experiment proves that GbAt11 transgenic cotton and GbAt11 transgenic arabidopsis have resistance which is remarkably superior to that of a non-transgenic wild type on verticillium, field verticillium wilt nursery evaluation proves that the anti-verticillium wilt phenotype of most transgenic strains is superior to that of the wild type, and yield is found to be improved after transgenosis at the same time. Therefore, the GbAt11 gene provided by the invention has important action on plant anti-verticillium wilt disease, and also has a very high application value for cultivating a novel plant variety with high verticillium wilt disease resistance.

The invention relates to the technical field of agricultural planting and discloses a soil conditioner and a preparation method. The soil conditioner is prepared from, in parts by weight, compound bacteria, an aid and 100-150 parts of selenium-rich organic fertilizer, wherein the compound bacteria comprise, in parts by weight, 0.1-0.5 parts of sheep tripe hyphae, 0.1-0.2 parts of aeromonas sobria, 0.2-0.5 parts of bacillus subtilis, 0.2-0.5 parts of bacillus thuringiensis, 0.2-0.5 parts of paenibacillus polymyxa and 0.1-0.2 parts of verticillium; the aid is prepared from, in parts by weight, 10-30 parts of quick lime and 10-20 parts of ground phosphate rock. The soil conditioner is applied to planting of grain crops in clay land and can effectively increase the selenium conversion rate of grain plants; the preparation method of the soil conditioner adopts a simple process and is low in cost.