35 results about "Vitaceae" patented technology

A fertilizer dedicated for gramineous plants features that it contains nucleotide besides well known N, P, K, B, Si and Mo. It can provide nutrients and trace elements to plants and promote the growth and development of plants. It can be suitable for bamboo by injection.

The invention relates to a production method of fermented vine tea, vine tea extract and vine tea drink. Tender stem leaves of ampelopsis humulifolia of vitaceae are taken as raw material, fermentation technique is adopted for production, and the production method comprises the steps of preparing vine tea, wetting the vine tea by adding water and steaming; preparing a seed culture medium, autoclaving, cooling, then inoculating edible funguses, and culturing at 28-40 DEG C for 24-120 hours to obtain seeds; inoculating seeds at the ratio of 3-8% of the weight of the vine tea, fermenting at 28-40 DEG C for more than 120 hours, and drying to obtain the fermented vine tea; and extracting, concentrating and drying to obtain fermented vine tea powder. The edible funguses in the invention comprise aspergillus niger, aspergillus oryzae, monacus anka, saccharomycetes, lactic acid bacteria and mixed strains. In the fermentation process of the vine tea in the invention, contents of active ingredients such as soluble flavone, polyphenol, tea pigment and the like are greatly improved, thus promoting absorption and utilization of the active ingredient of the vine tea and enhancing pharmacological efficacy of the vine tea. Besides, the production process of the invention thoroughly solves the technical problem that vine tea soup is precipitated after being cooled.

Methods of prophylaxis or treatment of a disease state initiated by or characterized by platelet aggregation, that employ a fruit extract or active fraction thereof, are disclosed. In one embodiment, the fruit extract or active fraction thereof, is obtained from the fruit of plants of the families Solanaceae, Rutaceae, Cucurbitaceae, Rosaceae, Musaceae, Anacardiaceae, Bromeliaceae, Vitaceae, Arecaceae, Ericaceae and Lauraceae. Pharmaceutical compositions comprising a fruit extract or active fraction thereof having platelet aggregation inhibitory activity are also disclosed.

Methods of prophylaxis or treatment of a disease state initiated by or characterized by platelet aggregation, that employ a fruit extract or active fraction thereof, are disclosed. In one embodiment, the fruit extract or active fraction thereof, is obtained from the fruit of plants of the families Solanaceae, Rutaceae, Cucurbitaceae, Rosaceae, Musaceae, Anacardiaceae, Bromeliaceae, Vitaceae, Arecaceae, Ericaceae and Lauraceae. Pharmaceutical compositions comprising a fruit extract or active fraction thereof having platelet aggregation inhibitory activity are also disclosed.

The invention provides an improved process for transforming gramineous crops by floral dip method, including the following steps. Firstly, agrobacterium with target gene is suspended by infiltration culture medium which contains Silwet L-17, Triton X-100 and GA. Secondly, under the condition of vivo, the integral inflorescence or partial pistils of the plants which are ready to be transformed are immerged in the infiltration culture medium, or the inflorescence or the pistils are sprayed with the infiltration culture medium, and the target gene is integrated with the genome of a target plant by the agrobacterium. By employing the genetically modified process, the invention resolves transformation problems of certain gramineous crops which are hard to be transformed by regular transforming method, thereby the invention has relatively high practical application value.

The invention discloses a beauveria bassiana DSXJ-07, which has been preserved with the accession number being CGMCC No.7192. The sequence of an internal trasicribed spacer of the beauveria bassiana DSXJ-07 is represented as the Seq ID No.7192. The invention also provides the application of the strain in prevention and treatment on pests on graminaceous crops, wherein the application is used for preparing a drug for preventing and treating the pests on graminaceous crops. The graminaceous crops include paddy rice, corn, sugarcanes, wheat, barley or forage grass. The pests comprise lissorhoptrus oryzophilus kuschel and asiatic corn borer. A pest killing fungus preparation prepared from the strain can effectively control the population quantity of the lissorhoptrus oryzophilus kuschel and asiatic corn borer.

The invention discloses a novel method for industrially preparing myricetin from stems and leaves of Ampelopsis sinica (Miq.) W.T.Wang of vitaceae Ampelopsis Michx or other plants of the vitaceae Ampelopsis. The novel preparation method includes the following steps: using alkaline liquor to adjust aqueous liquor or hydrous ethanol and methanol liquor into alkalescence to extract the total flavonoids in medicinal materials, condensing extraction liquor, using diluted acid to adjust the pH between 2 and 8, devitrifying and obtaining the crude extract; the crude extract is heated and extracted by using ethanol, methanol and acetone; the extraction liquor is decolored by active carbon; the myricetin can be obtained after devitrifying. The method has the advantages of simple technique, low cost, no pollution as well as being advanced, safe and practical.

The invention provides a gene for controlling a rice stem-leaf angle and application of the gene. The gene CYC U4;1(Os10g41430) disclosed by the invention specifically expresses in a rice pulvinus (believe that the gene also specifically expresses in other graminaceous crops), the CDS sequence of the gene is shown in the SEQ ID No.2, and a coded amino acid sequence is shown in the SEQ ID No.3. The invention further provides a promoter sequence, shown in the SEQ ID No.1, for the gene. A promoter of CYC U4;1 and overall length CDS are cloned to pCAMBIA1301, and then converted into Nipponbare, leaf-stem angles of the obtained transgenic lines are less than those of wild rice, therefore, plant type growth of plants can be controlled by increasing or decreasing expression quantity of CYC U4;1 gene through a genetic engineering technology, plant types are improved and germplasm density is increased.

The invention discloses mixed tea for preventing obesity and a preparation method of the mixed tea. The mixed tea is prepared from the following components in percentage by weight: 50%-90% of strigose hydrangea juvenile leaves and 10%-50% of vine tea, wherein the strigose hydrangea juvenile leaves are leaves and twigs of rubus suavissmus slee belonging to deciduous shrub rubus suavissimus S. Lee; and the vine tea is leaves of ampelopsis grossedentata of vitaceae. The taste of the strigose hydrangea juvenile leaves is too sweet; the taste of the vine tea is slightly astringent; the strigose hydrangea juvenile leaves have a good taste, so that the taste of other components can be effectively compensated; the tastes of the strigose hydrangea juvenile leaves and the vine tea can be effectively corrected; the quality and the tastes of the strigose hydrangea juvenile leaves and the vine tea are ensured; and the obesity can be relatively well prevented and treated by combination of the strigose hydrangea juvenile leaves and the vine tea.

The invention discloses a method for selecting elite seeds of a grass family by distant hybridization. The method mainly comprises the following steps: selecting a female parent and a male parent of the grass family which belong to the same species but different families and are in the same florescence; picking off staminate flowers of the female parent and hybridizing and selecting by pollen of the staminate flowers of the male parent containing protein of the female parent; then backcrossing for 2-3 times to obtain seeds with excellent characteristics of the male parent and the female parent finally. The method can be used for hybridization improvement of the characteristics of certain plants of the grass family by the excellent characteristics of the plants of other different families, thus obtaining a new variety with certain excellent characteristic that people desire. The method can help improve paddy rice into 'dry rice', thus realizing self-drought resistance of the paddy rice and easy plantation in an arid area.

A new orally administerable DHEA production promoter is provided. A composition containing an extract of at least one selected from the group consisting of plants of Rosaceae Crataegus, Saururaceae Houttuynia, Vitaceae Vitis, and Compositae Anthemis is provided as a DHEA production promoter. The extract may be of one of the plants or extracts of two or more of them may be used in combination. A specific example is preferably a mixture of extracts of all four of the aforementioned plants. This DHEA production promoter also can be used as, for example, an anti-aging agent such as a skin improver as well as a pharmaceutical agent such as an immunostimulator, an antidiabetic agent, an anti-osteoporosis agent, an antiarteriosclerotic agent, an anti-obesity agent, a sleep-promoting agent, and a central nervous system depressant.

The invention provides a planting method for improving the yield and quality of American ginseng. The planting method comprises the following steps of selecting the soil with the PH value of 4.5-7.5; ploughing once every half a month for drying the ridges, wherein the previous crops of the American ginseng are gramineous crops; during seed treatment, harvesting fresh American ginseng in August when the American ginseng is red in color, rubbing off the pulp, washing and drying, soaking the seeds by 1,000 times of 50% of carbendazim for 10-20 minutes, taking the soaked seeds out and airing, then soaking the seeds by 50 ppm good-quality gibberellin for 24 hours, then taking the seeds out and cleaning the seeds by clean water, stirring sand in the seeds at a ratio of seeds to sand of 1:3, accommodating the seeds mixed with the sand in a basin, a box and the like to be placed in a shade place until the November, controlling the temperature to be below 25 DEG C and controlling the moisture content to be 10-12%. The planting method for improving the yield and quality of American ginseng improves the yield and quality of the American ginseng under limited conditions.

The invention relates to a production method of fermented vine tea, vine tea extract and vine tea drink. Tender stem leaves of ampelopsis humulifolia of vitaceae are taken as raw material, fermentation technique is adopted for production, and the production method comprises the steps of preparing vine tea, wetting the vine tea by adding water and steaming; preparing a seed culture medium, autoclaving, cooling, then inoculating edible funguses, and culturing at 28-40 DEG C for 24-120 hours to obtain seeds; inoculating seeds at the ratio of 3-8% of the weight of the vine tea, fermenting at 28-40 DEG C for more than 120 hours, and drying to obtain the fermented vine tea; and extracting, concentrating and drying to obtain fermented vine tea powder. The edible funguses in the invention comprise aspergillus niger, aspergillus oryzae, monacus anka, saccharomycetes, lactic acid bacteria and mixed strains. In the fermentation process of the vine tea in the invention, contents of active ingredients such as soluble flavone, polyphenol, tea pigment and the like are greatly improved, thus promoting absorption and utilization of the active ingredient of the vine tea and enhancing pharmacological efficacy of the vine tea. Besides, the production process of the invention thoroughly solves the technical problem that vine tea soup is precipitated after being cooled.

The invention provides a myricetin extract, which is prepared by an extraction method comprising the following steps that (1) plants of myricaceae, vitaceae, leguminosae or theaceae are smashed; after degreasing, the smashed plants are dissolved in a methanol aqueous solution and are subjected to microwave and ultrasonic extraction; and an extraction solution is obtained; (2) the extraction solution obtained in the step (1) is subjected to pressure reduction concentration to obtain a concentrated solution; pressure-reduction suction filtration is performed; the solution is extracted by trichloromethane; the extraction solution is collected; and freeze drying and smashing are performed; (3) powder obtained in the step (2) is dissolved in petroleum ether and then passes through a silica gel column; purification is performed; eluate containing myricetin is collected; concentration is performed; and a myricetin crude product is obtained; and (4) the myricetin crude product obtained in the step (3) is added into the methanol aqueous solution with the volume ratio being 50 to 75 percent; heating is performed to 75 to 80 DEG C until the mixture is completely dissolved; stirring is performed; then, temperature reduction still standing, crystallization, suction filtration and drying are performed; and the myricetin extract is obtained. The myricetin extract can achieve a good effect of preventing or treating tumor metastasis after being taken for a long time, and has wide application prospects.

The invention discloses a vitaceae plant seedless growth regulator combination, a preparation and application of the preparation. The vitaceae plant seedless growth regulator combination comprises anactive ingredientcomponent A and an active ingredientcomponent B, the mass ratio of the active ingredientcomponent A to the active ingredientcomponent B is (500 to 6000):1, the active ingredientcomponent A is tungstic acid and / or tungstate, and the active ingredientcomponent B is gibberellic acid; before flowering, the seedless efficiency of seedy grapes can be improved by using the compound combination of the tungstic acid, the tungstate and the gibberellic acid, or the preparation; and the vitaceae plant seedless growth regulator combination and the preparation have a compound synergy effectin the aspects of promoting growing up of berries and increasing the weight of the grape berries.

The present invention provides an enhancer for enhancing the activity of oxidized protein hydrolase. The oxidized protein hydrolase activity enhancer according to the present invention contains an extract of at least one plant selected from the group consisting of Compositae Anthemis, Saururaceae Houttuynia, Rosaceae Crataegus, and Vitaceae Vitis.

The invention discloses a method for identifying gynostemma pentaphylla and making a distinction between gynostemma pentaphylla and cayratia japonica at deoxyribonucleic acid (DNA) level. The method is characterized by designing gynostemma pentaphylla SCAR marked specific primer pair WJS-T1 and WJS-T2 by using the result of medicinal plant cucurbitaceae gynostemma pentaphylla DNA characteristic segment SCAR marked clone sequencing, designing internal reference primer pair WHL-18S-C1 and WHL-18S-C2 according to a cucurbitaceae gynostemma pentaphylla 18S rRNA conserved region, forming a polymerase chain reaction (PCR) composite system 1, and amplifying the cucurbitaceae gynostemma pentaphylla DNA by using a touchdown PCR program to obtain two zones, namely a 359bp zone and a 293bp zone; anddesigning the internal reference primer pair WPT-18S-C1 and WPT-18S-C2 according to a vitaceae 18S rRNA conserved region, combining the internal reference primer pair WPT-18S-C1 and WPT-18S-C2 and the gynostemma pentaphylla SCAR marked specific primer pair WJS-T1 and WJS-T2 to form a PCR composite system 2, and amplifying a vitaceae cayratia japonica DNA by using the touchdown PCR program to obtain an 18S rRNA 177bp zone. Therefore, the DNA level of medicinal plant cucurbitaceae gynostemma pentaphylla is identified, and the distinction between the gynostemma pentaphylla and the vitaceae cayratia japonica which is easy to confound with the cucurbitaceae gynostemma pentaphylla in shape is made.

Disclosed is an activity enhancer for oxidized protein hydrolase. The activity enhancer for oxidized protein hydrolase is characterized by comprising an extract of at least one plant selected from the group consisting of plants belonging to Compositae Anthemis (Chamaemelum), Saururaceae Houttuynia, Rosaceae Crataegus and Vitaceae Vitis. It is preferable that the activity enhancer for oxidized protein hydrolase comprises an extract of a plant belonging to Compositae Anthemis (Chamaemelum), an extract of a plant belonging to Saururaceae Houttuynia, an extract of a plant belonging to Rosaceae Crataegus and an extract of a plant belonging to Vitaceae Vitis. It is also preferable that the plant belonging to Compositae Anthemis (Chamaemelum) is Anthemis nobilis L., the plant belonging to Saururaceae Houttuynia is Houttuynia cordata Thunberg, the plant belonging to Rosaceae Crataegus is Crataegus oxyacantha L. and the plant belonging to Vitaceae Vitis is Vitis vinifera L.

The invention relates to a planting method for radix scrophulariae. The method comprises the following steps of 1, land selection; 2, soil preparation; 3, base fertilizer application; 4, bedding; 5, transplanting, wherein the transplanting process is completed by adopting a semi-automatic transplanter; 6, topdressing, wherein the fertilization process is completed by adopting an automatic fertilization device, fertilization is performed on radix scrophulariae in the growth period so as to ensure nutrient supply to the radix scrophulariae, and then the growth speed of the radix scrophulariae isincreased; 7, weeding. In the land selection process, the land with the grass family or bean or pea family as the preceding crops is selected, continuous cropping of the radix scrophulariae on the same land is avoided, plant ash can be adopted as fertilizer, equivalently, base fertilizer is applied in advance, sufficient nutrients of soil are ensured, the soil is loosened in the soil preparationprocess, the air permeability is good, radix scrophulariae can conveniently absorb water, the hole forming process can be completed while transplanting is performed, and operation is convenient and rapid.

The invention relates to a Yao medicine rehabilitation external lotion for treating cerebral apoplexy sequelae, and a preparation method of the external lotion. The effective components of the external lotion are prepared from the following Yao medicine raw materials in parts by weight: 45-60 parts of Gnetum parvifolium (Warb.) C. Y. Cheng ex Chun, 45-60 parts of Semiliquidambar cathayensis Chang, 45-60 parts of vitaceae cissus hastataCissus pterocladaHayata, 45-60 parts ofPittosporum illicioides Mak., 45-60 parts of Acanthopanacis Cortex, 25-40 parts ofGlechomalongituba (Nakai) Kupr., 45-60 parts of Kadsura coccinea (Lem.) A. C. Smith, 45-60 parts of MillettiareticuiataBenth, 45-60 parts of Herba Sarcandrae, 45-60 parts ofGaultheria cumingiana, 45-60 parts of Liquidambar formosana, 45-60 parts of Ventilago leiocarpa Benth., 45-60 parts of Pinus massoniana Lamb., 45-60 parts of Sargentodoxa cuneata (Oliv.) Rehd. Et Wils. and 45-60 parts of Grendarussa valgaris. The external lotion has the characteristics that the cerebral apoplexy sequelae can be effectively treated; the external lotion has a very treatment effect for waist-leg pain, limb numbness, intermittent claudication and skeleton pain caused by lumbar disc herniation; the effects of treating both symptoms and root causes are achieved; the body immunity can be enhanced; and the like.

The invention discloses a tread-resistant walking lawn. The lawn comprises a support layer and a planting layer positioned on the support layer; plants planted in the planting layer comprise gramineous bottom pasture and turfgrass, wherein the ratio of the gramineous bottom pasture accounts for 10-13 % of the mixed grass, the gramineous bottom pasture comprises one or a combination of more selected from leymus chinensis, elyrnus dahuricus, elymus sibiricus, bromus inermis, eragrostis curvula, festuca ovina and cogongrass, and coconut shreds with a usage amount of 50-60 kg / mu are uniformly distributed in a root system planting matrix layer of the planting layer. The construction method comprises the following steps: laying the planting layer on the support layer, and planting the mixed grass on the planting layer. Through the good mutual growth promotion between the gramineous bottom pasture and the common turfgrass, the tread-resistant performance of the lawn is greatly increased withthe combination of the improvement of a root system planting matrix by the coconut shreds.

The invention discloses a method for testing the growth of fungal hyphae in plant stems. The method includes the following steps: (1) taking a glass tube with a diameter of 10-50 mm, forming ventilation holes at intervals of 3-5 mm on the side wall of the glass tube, and controlling the diameter of the ventilation holes to be less than 0.2 mm; (2) sealing the bottom of the glass tube with a filterscreen, wherein the diameter of filter holes of the filter screen is 0.2-0.3 mm, placing the bottom of the glass tube into a culture dish, and pouring culture liquid into the culture dish; (3) pouring glass beads with a diameter of 0.4-0.5 mm into the glass tube; (4) pouring transparent nutrient solution inoculated with the hyphae into the glass tube filled with the glass beads, pouring once every 20-30 hours, and continuously pouring 1-2 times; (5) placing the glass tube together with the culture dish into a culture chamber for culture for 7-20 days; (6) observing and recording the growth ofthe hyphae in the glass tube to obtain a growth mode of the hyphae. The method is used for researching the growth condition of the fungal hyphae when fungal pathogens cause stem mycosis of gramineousplants.

The invention belongs to plants or methods for obtaining new plants, and discloses a method for obtaining new rice germplasm through utilizing fargesia DNA (deoxyribonucleic acid) to combine with a pollen tube channel in the technical field of plant regeneration through tissue culture technology. The method comprises the following steps of treating a rice variety Enhui 142 to generate mutation byutilizing a method by which grass family arundhoriaalpha whole genome DNA combines with the pollen tube channel, screening single plants of which the phenotypes are obviously different from those of reference samples in 318 seedlings generated from 450 glumous flowers to perform continuous selfing to obtain 13 mutant strains with stable phenotypes; analyzing the mutant strains and the reference samples by utilizing 48 pairs of SSR molecular markers, and discovering that the genetic background between the mutant strains and the reference samples and the genetic background between the mutant strains both have differences; enabling mutant materials and the reference samples to be respectively subjected to hybridization and matching with photo-thermo-sensitive genic male sterile lines. The method for utilizing the fargesia DNA to combine with the pollen tube channel can be used for creating new germplasm for rice breeding, and provides a new approach for the germplasm resource innovation of rice.

The invention discloses a fungal hypha growth test method in gramineous plant stalks. The method comprises the following steps of (1) taking a glass tube with the diameter being 10 to 50mm; forming ventilation holes every 3 to 5mm in the side wall of the glass tube; and controlling the diameter of each ventilation hole to be smaller than 0.2mm; (2) sealing the bottom of the glass tube by a filterscreen, wherein the filter hole diameter of the filter screen is 0.2 to 0.3mm; (3) pouring glass beads with the diameter being 0.4 to 0.5mm into the glass tube; (4) inoculating fungal hyphae onto thefilter screen at the bottom of the glass tube; then putting the bottom of the glass tube into a culture vessel; and pouring a culture solution into the culture vessel; (5) putting the glass tube and the culture vessel into a culture chamber to be cultured for 14 to 30d together; and (6) observing and recording the growth condition of the hyphae in the glass tube to obtain the hypha growth mode. The method is used for studying the fungal hypha growth condition when the gramineous plant stalk fungal disease is caused by fungal pathogenic bacteria.

The invention provides a method for decreasing the mildewing rate of elymus sibiricus seeds in the germination stage. The method comprises the steps that the elymus sibiricus seeds are subjected to priming in water at the constant temperature of 40+ / -1 DEG C and then subjected to natural dehydration, and the seeds obtained after natural dehydration are subjected to seed soaking treatment for a period of time with Neotyphodium sp. liquid. The method has the advantages that safety is achieved, wherein Neotyphodium sp. separated from gramineous plants is utilized, and therefore not only can high efficiency of the Neotyphodium sp. be guaranteed to the maximum extent, but also safety and non-toxicity to the ecological environment can be guaranteed; high efficiency is achieved, wherein warm water bath priming is conducted on the seeds while seed-carried fungi are killed, and therefore high efficiency and rapidness are achieved; operation is easy and convenient, wherein the steps are simple and comprise constant-temperature water bath priming, seed dehydration and seed soaking through fungus liquid; low damage is generated to seed vitality, wherein due to the fact that the constant-temperature water bath priming temperature is low and the treatment time is short, no influence can be generated to seed germination, the germination potential of the seeds can be improved to a certain extent, and the germination rate of the seeds can be increased to a certain extent; the mildewing rate of the seeds is significantly decreased.

The invention relates to a universal SNP typing probe in gramineous plants. The probe is composed of two pairs of reverse complement oligonucleotide sequences, and a fluorescence reporter group and a fluorescence quenching group are respectively positioned in reverse complement oligonucleotide chains. A present Taqman probe method is improved to design the SNP typing universal Taqman probe, so the experiment cost is reduced, and a Taqman probe SNP typing method can be widely applied in SNP detection of the gramineous plants.

The invention discloses a method for selecting elite seeds of a grass family by distant hybridization. The method mainly comprises the following steps: selecting a female parent and a male parent of the grass family which belong to the same species but different families and are in the same florescence; picking off staminate flowers of the female parent and hybridizing and selecting by pollen of the staminate flowers of the male parent containing protein of the female parent; then backcrossing for 2-3 times to obtain seeds with excellent characteristics of the male parent and the female parent finally. The method can be used for hybridization improvement of the characteristics of certain plants of the grass family by the excellent characteristics of the plants of other different families,thus obtaining a new variety with certain excellent characteristic that people desire. The method can help improve paddy rice into 'dry rice', thus realizing self-drought resistance of the paddy riceand easy plantation in an arid area.

The invention provides an improved process for transforming gramineous crops by floral dip method, including the following steps. Firstly, agrobacterium with target gene is suspended by infiltration culture medium which contains Silwet L-17, Triton X-100 and GA. Secondly, under the condition of vivo, the integral inflorescence or partial pistils of the plants which are ready to be transformed areimmerged in the infiltration culture medium, or the inflorescence or the pistils are sprayed with the infiltration culture medium, and the target gene is integrated with the genome of a target plant by the agrobacterium. By employing the genetically modified process, the invention resolves transformation problems of certain gramineous crops which are hard to be transformed by regular transformingmethod, thereby the invention has relatively high practical application value.

The invention discloses nutrient soil for flowers, relating to the technical field of biofertilizers. The nutrient soil is composed of the following components in percentage by mass: 20% of leaf mould, 20% of mountain clay, 30% of soil, 2% of chicken manure, 18% of saw dust, 9% of bulking agent, 0.5% of preservative and 0.5% of plant nutrients. The nutrient soil is rich in nutrient, low in preparation cost, favorable in sterilization and insecticide functions and suitable for households; in addition, the nutrient soil can provide relatively-large proportional long-acting solid-phase organic matters, a stable pH value and favorable water and air control performance for plants, so that the plants are more convenient in cultivation management, the plants are promoted to be rapidly rejuvenated after the roots of the plants grow, the nutrient requirements of the flowers can be met, and the environment can not be polluted; the soil can be used for most of gramineous plants and a part of woody plants with short branches and compact crowns.