77results about How to "Increase cumulative" patented technology

An alternating current driven type plasma display device comprising a first panel and a second panel, said first panel having sustain electrodes formed on a first substrate and a dielectric material layer formed on the first substrate and the sustain electrodes, wherein the first panel and the second panel are bonded to each other in their circumferential portions,characterized in that the dielectric material layer has a thickness of 1.5x10<-5 >m or less.

An alternating current driven type plasma display device comprising a first panel and a second panel, said first panel having sustain electrodes formed on a first substrate and a dielectric material layer formed on the first substrate and the sustain electrodes, wherein the first panel and the second panel are bonded to each other in their circumferential portions, characterized in that the dielectric material layer has a thickness of 1.5x10-5 m or less.

The invention provides a pH and Redox dual-response amphiphilic block copolymer as well as a preparation method and application thereof. The molecular formula of the copolymer provided by the invention is MPEG-Linker-PAE-ss-PLGA and the copolymer has a structure as shown in the formula I and can be prepared through the following steps of performing reaction to modify macromonomer methoxypolyethylene glycols (MPEG), thereby obtaining hydrophilic macromonomer (MPEG-Linker) with pH responsiveness; performing reaction to modify macromonomer polylactic acid-poly lactic-co-glycolic acid (PLGA) by use of disulfide bonds, thereby obtaining a hydrophobic macromonomer (PLGA-Cys) with Redox responsiveness; and performing michael addition reaction through a one-step method so as to obtain the copolymer. The copolymer provided by the invention can be used in systems for loading water-insoluble drug micelle, is capable of controlling slow release of loaded drugs in normal tissues, is low in release amount accumulated for a long time, and can be enriched and rapidly released in focus tissues, thereby improving the bioavailability and treatment effect of the medicaments.

The invention discloses an ecology and environment-friendly soil improvement agent, which is prepared from the following raw materials in parts by weight: 15-25 parts of ore soil filler, 7-11 parts of coal gangue, 8-10 parts of straw, 10-20 parts of mixed grass meal, 3-5 parts of compound microorganisms, 5-7 parts of humate, 8-12 parts of excrement of livestock, 4-6 parts of desulfurized gypsum, 6-8 parts of bamboo charcoal powder and 5-7 parts of trace element additives. The soil improvement agent provided by the invention can effectively overcome the imbalance of nutrient elements in soil, improve soil fertility, loosen the soil, improve fertilizer efficiency, increase the contents of organic matters and nutrients, loosen hardened soil, accelerate a process of raw soil ripening and improve or recover land productivity; furthermore, industrial and agricultural wastes are used as most of the raw materials, so that the soil improvement agent is environmentally-friendly and safe; furthermore, the preparation method provided by the invention is lower in material cost, easy in obtaining of raw materials, simple in technology and easy to operate and in realization of industrial production, thus having higher practical value and a good application prospect.

The invention provides a biosynthesis method for increasing accumulation of L-5-methyltetrahydrofolate, and an L-5-methyltetrahydrofolate synthetase system co-expressed recombinant plasmid and a construction method and application thereof. The L-5-methyltetrahydrofolate synthetase system co-expressed recombinant plasmid comprises DHFR (dihydrofolate reductase) gene folA and a MTHFR (methylenetetrahydrofolate reductase) gene metF sequence. The biosynthesis method for increasing accumulation of L-5-methyltetrahydrofolate includes converting the L-5-methyltetrahydrofolate synthetase system co-expressed recombinant plasmid to accumulate an original strain of the L-5-methyltetrahydrofolate so as to obtain a recombinant strain, and fermenting the recombinant strain. The accumulation of the L-5-methyltetrahydrofolate in final fermentation product is evidently higher than that of the L-5-methyltetrahydrofolate in the original strain. Utilization rate of raw materials is increased, production cost and energy consumption are reduced, and a foundation for industrial biosynthesis of the L-5-methyltetrahydrofolate is laid.

The invention relates to double-pH-response amphiphilic copolymer, the molecular formula of the double-pH-response amphiphilic copolymer is MPEG-Dliable-PAE-g-Chol, and the structure of the double-pH-response amphiphilic copolymer is as shown in formula I. The double-pH-response amphiphilic copolymer is copolymerized by hydrophilic block methoxy polyethylene glycol, hydrophobic cholesterol and pH response block poly(beta-amino ester). The double-pH-response amphiphilic copolymer has the advantages that the double-pH-response amphiphilic copolymer can self-assemble in an aqueous solution to obtain a nanoscale micellar system, the inner layer of the nanoscale micellar system is a hydrophobic core modified by cholesterol, the middle of the nanoscale micellar system is a pH-sensitive-response PAE layer, the shell of the nanoscale micellar system is hydrophilic block MPEG, the hydrophilic shell is connected sensitive middle layer through a pH-sensitive benzimide bond, the cell intake of a micelle drug-loading system is increased effectively while the requirements of high entrapment performance, stable system structure, long in-vivo circulation time, stability under neutral conditions and controllable drug release under weak acid conditions of hydrophobic drugs are satisfied, and accordingly drug bioavailability is increased, and a tumor treatment effect is optimized.

The invention relates to plant chelating and inducing plant restoring technology for soil polluted by heavy metal cadmium and discloses an application of a chelating agent (namely APAM (Anionic polyacrylamide)) for reinforcing amaranthus hypochondriacus to restore the soil polluted by heavy metal cadmium. The specific steps are as follows: the amaranthus hypochondriacus is planted in the soil polluted by the heavy metal cadmium, and 8.33-33.33Kg of chelating agent (APAM) is applied in each 666.7m<2> of the soil polluted by cadmium before 15-25 days when the amaranthus hypochondriacus is harvested, the specific mode is as follows: the APAM is dissolved in water and is applied in the soil in the soil surface spaying manner so as to improve the biological effectiveness and promote the capability of the plant to absorb the heavy metal cadmium, so that the purposes of increasing the accumulation amount of the heavy metal cadmium and improving the heavy metal cadmium extracting efficiency are achieved. The amaranthus hypochondriacus has the advantages of large organism amount, cadmium forcing resistance and uniform gathering property and the like, and the chelating agent (APAM) has the function of activating the heavy metal cadmium and is low in cost, and due to the combination of the amaranthus hypochondriacus and the APAM, the restoring efficiecy of the amaranthus hypochondriacus is improved, and the capability of restoring the soil polluted by the heavy metal cadmium is enhanced.

The invention discloses a nanoreactor as well as a preparation method and application thereof. The nanoreactor comprises core-shell nanoparticles, manganese dioxide and a targeting ligand, wherein the surfaces of the core-shell nanoparticles are coated with the manganese dioxide and the targeting ligand layer by layer; the core-shell nanoparticles are formed by a metal organic framework, a photosensitizer, glucose oxidase and a polylactic acid-glycolic acid copolymer nano core wrapped by the metal organic framework, and the metal organic framework is formed by tannic acid and iron through coordination; and the targeting ligand is one or more of hyaluronic acid, synthetic polypeptide, a folic acid modified hydrophilic polymer and a nucleic acid aptamer. The nanoreactor can actively target to tumor cells, catalyzes oxygen production to realize in-situ oxygen supply of tumors, removes glutathione and ATP so as to inhibit and overcome tolerance, and enhances the photodynamic therapy efficiency. The preparation method is simple and effective, effectively avoids the toxicity of a carrier, is high in drug loading capacity and modification rate, and can be applied to preparation of tumor targeting drugs.

The invention provides an application of saccharomyces cerevisiae in biosynthetic L-phenylacetylcarbinol. The application comprises the steps of obtaining formaldehyde tolerant yeast strains, constructing a biocatalyst, converting microorganisms and the like. According to a method, one the one hand, the yeast strains are screened to obtain the yeast strain which is high in tolerance to benzaldehyde and stable in growth trait; on the other hand, a composite carrier stabilized benzaldehyde tolerant yeast strain is used for improving the metabolism condition of thallus, the catalyzing time of enzyme is stabilized and prolonged, the reaction condition is improved, the concentration of key substrate benzaldehyde is improved, the separation procedure of the thallus and conversion liquid is simplified, and therefore the cumulant of L-PAC is improved, wherein the concentration of L-PAC in the conversion liquid is close to 45 g / L. According to the method, the utilization of the raw materials is greatly improved, production cost is reduced, the yield of L-PAC is remarkably increased, and the foundation can be laid for industrialization of biologically synthesized ephedrine.

The invention discloses functional and environment-friendly compounded planting nutritional soil and a preparation method thereof. The nutritional soil comprises raw materials in parts by weight as follows: 5-7 parts of modified biochar, 15-25 parts of modified filled earth, 5-7 parts of shell powder, 10-15 parts of cake fertilizer, 1-3 parts of a botanical insecticide, 8-12 parts of livestock excrement, 7-11 parts of agricultural straw, 0.1-0.3 parts of a growth regulator, 2-4 parts of a sterilization additive, 4-6 parts of rare earth micro-fertilizer and 6-10 parts of a moisture preserving additive. The nutritional soil is rich in nutrients, further has various functions of killing pests and bacteria, preserving moisture, improving soil and promoting growth and has higher practical valueand good application prospect, productivity of a land can be improved or restored, industrial and agricultural waste is abundantly used as the raw material and is environmentally friendly and safe, and operation and industrial production are easy.

The invention discloses an Setophoma terrestris D2G24 strain. The preservation number of the Setophoma terrestris D2G24 strain in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 20268. The strain is applied to bioremediation of heavy metals lead, zinc and cadmium. Under the stress of heavy metal pollution, the strain can promote plant growth, promote the transfer capacity of plants to heavy metals, has relatively strong accumulation capacity to heavy metals cadmium and zinc, and is suitable for in-situ remediation of large-area heavy metal cadmium and zinc pollution.

The invention discloses an Epicoccum nigrum FZT214. The preservation number of the Epicoccum nigrum FZT214 in the China General Microbiological Culture Collection Center is CGMCC No. 21045. The strainis applied to promotion of plant seed germination and seedling growth, and experiments prove that the strain can promote the plant seed germination and seedling growth, increase the plant seed yieldand enhance the accumulation capacity for heavy metal cadmium under the stress of heavy metal pollution, and is suitable for in-situ remediation of large-area heavy metal cadmium polluted soil Cd.

The invention discloses a cholesterol grafted pH-response tri-block amphiphilic copolymer and a preparation method and application thereof. Through Michael addition, a pH-response macromonomer poly (beta-amino ester) (PAE) is prepared; through Michael addition, a pH-response tri-block amphiphilic intermediate product PAE-b-PEG-b-PAE is prepared; and finally cholesterol is grafted onto PAE-b-PEG-b-PAE to obtain the cholesterol grafted pH-response tri-block amphiphilic copolymer Chol-g-PAE-b-PEG-b-PAE-g-Chol. The cholesterol grafted pH-response tri-block amphiphilic copolymer is applied in the preparation of a micelles system for loading poorly water-soluble drugs. The micelles system can be self-assembled in an aqueous solution to form nano-micelle with a stable structure and can efficiently coat and load poorly water-soluble drugs. By adjusting the ratio of different blocks in the polymer, pH response range can be effectively adjusted. Then, micelle can rapidly and accurately respond to pH changes of an environment, and burst release also can be effectively relived to control drug release rate.

The invention belongs to the technical field of nano probes, and discloses a double-organelle-targeted nano probe as well as a preparation method and application thereof. The double-organelle-targetednano probe is mainly prepared from a compound of formula I and a photo-sensitive agent II; and the photo-sensitive agent II is phthalocyanines or porphyrins photo-sensitive agent with negative charges. The compound of formula I is a mitochondria-targeted chemotherapy reagent with an aggregation-induced luminescent effect. The nano probe of the invention not only can monitor the release process ina cell in real time by virtue of a double-fluorescent illumination way, but also can effectively improve the killing efficiency for cancer cells by virtue of the synergism of the mitochondria-targeted chemotherapy and lysosome-targeted photodynamics therapy. The invention also relates to application of nano probe in preparing an antitumor drug and / or fluorescent imaging. (The formulas are shown in the description).

The invention discloses a local double regulation method for application of starter fertilizer and top dressing for maize, which includes the steps: applying the starter fertilizer to one side of each seeding row by furrow application; and applying the top dressing to one side of each plant row by furrow application during maize jointing, namely the side opposite to the starter fertilizer; wherein each of the starter fertilizer and the top dressing is composed of nitrogen fertilizer and phosphate fertilizer. By combining double local regulation of the starter fertilizer and the top dressing according to growth characters of maize, and compared with traditional application of fertilizer by farmers, the method meets the requirement of maize on nutrients during key growth period, fertilizingefficiency is improved, and both production and income are increased. A scientific high-yield and high-efficiency fertilizing method for maize is provided, and is effective. Especially, with popularization of agricultural mechanization, higher practical application value is achieved by combining fertilizing technology with agricultural machinery. Meanwhile, the combination of agriculture and agricultural machinery leads the development of modernized agriculture and is popularized internationally. The method has broad application prospect on domestic maize production.

The invention provides a method for enhancing the restoration capacity of chickweed on cadmium-polluted soil in an orchard through uniconazole. The method includes the following steps that chickweed rooted due to cutting is transplanted into the cadmium-polluted orchard soil, watering is performed every day, and after 30 days, the uniconazole with the concentration being 10-80 mg / L is sprayed to the chickweed. By means of the method, the cadmium content of the overground portion of the chickweed can be remarkably increased, and the increasing range can reach up to 39.1%; and the cadmium accumulative amount of the overground portion of the chickweed can be increased by 10.47%. By means of the method, the extraction capacity of the chickweed on cadmium in the cadmium-polluted soil in the orchard is remarkably improved.

A culture method for accumulating organic iodine in Dunaliella salina achieves the effects of promoting the growth of the Dunaliella salina and increasing the cumulant of Beta-carotene and organic iodine by changing Dunaliella salina culture conditions. The biomass and Beta-carotene cumulant of the finally obtained Dunaliella salina are more than 1.5 times as much as the biomass and Beta-carotene cumulant of the conventionally cultured Dunaliella salina, and the content of the organic iodine in the alga reaches 110.6mg / kg by dry weight. The culture method shortens the Dunaliella salina culture time, the culture conditions can be easily controlled, the production cost is low, and the added value of products is high. Substances added in the whole culture process come up to national regulations, so the product is safe and reliable, and can be applied in food and pharmaceutical industries.

The invention discloses a preparing method of 5' -nucleic acid in biochemical engineering technical, which comprises the following steps: 1, utilizing reconstructing DNA technique; constructing acid phosphatase expressing carrier; transforming bacillus coli; getting genetic engineering bacteria of high effective expressing acid phosphatase; 2, abrupt-changing acid phosphatase gene; sieving acid phosphatase with higher compatibility; 3, catalyzing nucleoside with acid phosphatase; synthesizing 5' -nucleic acid with phosphoric acid group donator. This product possesses short period and low cost, which is fit for commercial manufacture.

The application provides an acoustic model training method, device, computer equipment and readable storage medium, wherein the method comprises the following steps: obtaining feedback data corresponding to an initial acoustic model during use of the initial acoustic model, wherein the feedback data comprise positive feedback data for characterizing accurate identification results of the initial acoustic model, and negative feedback data for characterizing incorrect identification results of the initial acoustic model; and modifying and training the initial acoustic model based on the feedbackdata and original training data corresponding to the initial acoustic model. By adopting the method, the utilization of the feedback data actually collected by a client as training data can be realized, so that both time and labor costs are saved; in addition, since the feedback data are homologous with the client, the occurrence of biased training can be avoided and the performance of the acoustic model can be improved.

The invention belongs to the technical field of biochemical engineering, relates to an improved method for producing 5'-flavor nucleotide and particularly relates to a method for constructing a gene engineering bacterium through a DNA (deoxyribonucleic acid) recombination technology, carrying out mutant screening on the gene engineering bacterium and utilizing the high-activity gene engineering bacterium to produce 5'-nucleotide. The method comprises the following steps: 1, through the DNA recombination technology, constructing an acid phosphatase expression vector, and converting into Escherichia coli to obtain a gene engineering bacterium capable of efficiently expressing acid phosphatase; 2, mutating the gene of the acid phosphatase, and screening the acid phosphatase having high affinity with nucleoside; and 3, using the acid phosphatase to catalyze the nucleoside and a phosphate group donor so as to synthesize the 5'-nucleotide. By using the method to realize the catalytic synthesis of the 5'-nucleotide, the invention has the characteristics of simple process, high efficiency, short period, less bacterium consumption, low cost and the like, conforms to environment protection requirements, and is suitable for industrial production.

The invention discloses a plant endophytic fungus phomopsis (Phomopsis columnaris) D2G7. The preservation number of the plant endophytic fungus phomopsis cloumnaris D2G7 in the China General Microbiological Culture Collection Center is CGMCC No. 21044. The strain is applied to bioremediation of heavy metals lead, zinc and cadmium; under the stress of heavy metal pollution, the strain can promote plant growth, promote the transfer capacity of plants to heavy metals, has strong accumulation capacity to heavy metals cadmium and zinc, and is suitable for in-situ remediation of large-area heavy metal cadmium and zinc pollution.

The invention discloses a carbon-based compound fertilizer with a high potassium utilization rate. The preparation method comprises the following steps: according to nutrient requirements of flue-cured tobacco, preparing a base fertilizer according to a scientific and reasonable proportioning method; preparing a carbon-based compound fertilizer according to a ratio 7:3 of the base fertilizer to biomass charcoal, mixing into an organic adhesive prepared from 2.5% of sodium alga acid, chitosan or wood vinegar, fully and uniformly mixing, and taking out the mixture; preparing spherical particles with the particle size of less than 6mm from the mixture by using a granulator, and drying at a low temperature, thereby obtaining the carbon-based compound fertilizer. The carbon-based compound fertilizer disclosed by the invention has the advantages that (1) the nutrition is comprehensive and rich, one-time topdressing is performed in the uncovered ridging period during flue-cured tobacco cultivation, the operation is convenient, and the yield and output value of the flue-cured tobacco and the ratio between medium and high-grade tobacco leaves can be obviously improved; (2) the release rate of potassium in the fertilizer in soil is retarded, the effectiveness of the potassium is improved, and the potassium content in the tobacco leaves, potassium accumulation in the flue-cured tobacco and the potassium utilization rate in the flue-cured tobacco fertilizer are obviously improved; and (3) the carbon-based compound fertilizer is readily available in raw materials, environment-friendly and safe.

The invention discloses a method for increasing the fruit setting rate of large cherries suitable for southwest highlands. The method includes the following steps that a base fertilizer is applied sufficiently in autumn, an orchard is subjected to pruning, leaves are removed manually, a lime-sulphur mixture diluent is sprayed for disinfecting the orchard, the stand columns are installed, and a shading net is put in the orchard; the orchard is watered before trees germinate at the beginning of spring, and then the ground projection areas of tree crowns are covered with mulching films; a homemade fruit-setting agent is sprayed during the blooming period and the early fruit-setting period of the large cherries; after the fruit-setting period of the large cherries is over, the shading net is removed in time; during the large cherry fruit growth period, watering is carried out in time, and a potassium dihydrogen phosphate solution is sprayed for foliage top-dressing; and in addition, whether insects and diseases occur or not needs to be paid attention to, and comprehensive prevention and control is carried out according to the characteristics of the occurrence of insects and diseases. The method is simple and easy to operate, and convenient to operate, the fruit-setting rate and yield of large cherries can be effectively improved, tests show that the fruit-setting rate can be increased by 40.50%, and the yield can be increased by 52.80%.

The invention provides a method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid (DHA). The method comprises the following steps: with a heterotrophic culture medium of crypthecodinium cohnii as a foundation, adding any chemical promoter into the heterotrophic culture medium; the heterotrophic culture medium is composed of 9g / L of glucose, 2g / L of yeast extract powder and 25g / L of sea salt; the chemical promoter can also be called a plant growth regulator, including indoleacetic acid (IAA), naphthoxyessigsaeure (BNOA), chlorobenzoic acid, salicylic acid (SA), abscisic acid (ABA) and cholamine, and the concentrations of these chemical promoters in the heterotrophic culture medium are as follows: 2.5mg / L of indoleacetic acid (IAA), 4mg / L of naphthoxyessigsaeure (BNOA), 4mg / L of chlorobenzoic acid, 1mg / L of salicylic acid (SA), 20mg / L of abscisic acid (ABA) and 2.5mM cholamine; culturing at 25 DEG C for 72 hours, extracting grease, and measuring the DHA content of the culture by using a gas chromatograph-mass spectrometer, meanwhile, extracting cellular metabolite from a parallel culture, and detecting and analyzing the content change condition of the cellular metabolite by using a metabonomics method.

The invention belongs to the technical field of food processing, and particularly provides a preparation method of gamma-aminobutyric acid-enriched germinated brown rice soft snow cakes, which comprises the following steps: step 1) preparing germinated brown rice powder; step 2) preparation of cured germinated brown rice powder: blending and stirring the germinated brown rice powder and water to form germinated brown rice powder milk, and subjecting the germinated brown rice powder milk to microwave treatment to obtain a cured germinated brown rice powder emulsion, wherein the microwave treatment parameters are that the power is 400-800 W, the time is 10-30 min, and the temperature is 10-50 DEG C, and drying and crushing the cured germinated brown rice powder emulsion to obtain cured germinated brown rice powder; (3) preparing cured germinated brown rice enzyme powder: preparing a cured germinated brown rice enzyme culture medium, inoculating a pre-activated leavening agent into the cured germinated brown rice enzyme culture medium, fermenting, drying and crushing to prepare the cured germinated brown rice enzyme powder; 4) preparing the germinated brown rice soft snow cake: premixing the cured germinated brown rice enzyme powder and the soft snow cake preparation raw materials to prepare dough, and fermenting, baking, seasoning and cooling the prepared dough to prepare the soft snow cake.

The invention provides a haematococcus pluvialis high-efficiency mariculture method. On the basis of a traditional two-step method, culture conditions are adjusted reasonably, seawater is added for supplementing natural nutritional ingredients for algae, and rich seawater resources in Hainan and other coastal areas are fully utilized; a pumpkin seed powder, a sweet potato powder and a brainea insignis ethanol extract are added in a culture medium, sufficient nutritional ingredients are supplemented, and a role in preventing biological pollution is played; at the same time, through alternativeutilization of mixed light sources, algal propagation and astaxanthin accumulation can be effectively promoted. With adopting of the method, the cell density is as high as 10.56*10<5> / ml, the dry weight of cells is as high as 3.15 mg / ml, the astaxanthin content is as high as 104.12 mg / g, and the single-cell astaxanthin content is as high as 3.51*10<-2> [mu]g per cell. The method not only can effectively improve the reproductive capacity of haematococcus pluvialis, and moreover, the accumulation of the single-cell astaxanthin is significantly increased.

The invention discloses a local-regulation-based fertilizing method for seed manure special for corns, which comprises the following step of applying the seed manure special for the corns at one side of each sowing row while sowing, wherein the seed manure special for the corns is composed of a nitrogen fertilizer, a phosphate fertilizer and a sulfur fertilizer; a horizontal distance between the seed manure special for the corns and each sowing row is 5cm-6cm; and a vertical distance between the seed manure special for the corns and corn seeds is 5cm-6cm. According to the growth characteristic of the corns, compared with the habitual manner of applying the seed manure by peasants, the local-regulation-based fertilizing method has the advantages that the efficiency of the manure is increased, the growth in a seedling stage is boosted and the yield is increased. The invention provides a scientific fertilization technology for realizing high yield and high efficiency of the corns; the effect of increasing production is obvious; particuarlly, with the popularization of agricultural mechanization, the seed manure technology is combined with agricultural machinery, so that the practicalapplication value is wider; the combination of agricultural technologies and agricultural machinery is the development direction of modern agriculture and is popularized internationally; and the method disclosed by the invention has a wider market prospect in corn production in China.