38results about How to "Mild culture conditions" patented technology

The invention discloses a bacillus sphaericus with high yield and high-temperature xylanase resistance and an application thereof. The bacillus sphaericus is classified and named as Lysinibacillus sphaericus Xyn-1, and is collected in China Center for Type Culture Collection (CCTCC) on May, 5th, 2014 with the collection number CCTCCM2014183. The optimal fermentation enzyme production condition of a strain screening shake flask is determined by adopting a Plackett-Burman design and a response surface analysis method, and the fermentation level under the condition can reach 5678.5IU / mL. When the enzyme produced by fermentation of the strain is applied to degradation of plant hemicellulose, the xylobiose yield of the hemicellulose can reach over 60% through enzymolysis.

The invention discloses a preparation method of a squalene synthetase inhibitor CJ-13982. The compound CJ-13982 is separated and prepared from a fermentation culture of nigrospora oryzae A8 which has a collection number of CCTCC NO. M 2011412. The invention provides a method for preparing the compound CJ-13982 with the squalene synthetase inhibiting effect from the fermentation culture of a plant endophytic fungi nigrospora oryzae A8. The method is advantaged in mild culturing condition, simple and feasible culturing medium preparation, simple separation and purification steps, and high yield. With the method, large-scale production can be realized in a manual controlling condition. With the method, a problem of raw material required by compound CJ-13982 researches or applications can easily be solved, and a novel approach is provided for the preparation of the compound CJ-13982.

The invention discloses a biological transformation and synthesis method of rubescensin. The synthesis method comprises the following steps of: firstly, preparing a solid culture medium and a liquid culture medium; then, sterilizing and then inoculating the leaves, leaf buds or rhizome shucks of rabdosia rubescens into the prepared solid culture medium for culturing; transferring the callus obtained by culturing into the liquid culture medium for culturing; extracting the callus cultured in the liquid culture medium by adopting an organic solvent; and removing the organic solvent after extracting to obtain a rubescensin product (kaurene type diterpenoid compound). In the invention, the technical scheme for culturing and preparing rubescensin is free from the restriction of natural resources and is favorable for protecting the ecological environment. The technical scheme disclosed by the invention is easy to realize industrialized production, has the advantages of shorter culture time and higher product yield, lowers the production cost, and has obvious economic and social benefits.

The invention relates to the technical field of terpenes and provides a compound peniroquesine A with anti-inflammatory activity. The structure of the compound is shown as formula I in the description. The compound belongs to sesterterpenes and has remarkable anti-inflammatory activity. The compound peniroquesine A with the anti-inflammatory activity is sesterterpenes with novel 5 / 6 / 5 / 6 / 5 five-ring skeleton structure, and variety of the sesterterpenes is enriched. The invention further provides a preparation method of the compound peniroquesine A with anti-inflammatory activity. The compound peniroquesine A is prepared from Penicillium roqueforti by fermenting. The method is simple, fast, low in cost and suitable for large-scale industrial production.

The invention belongs to the technical field of fermentation engineering, in particular to a method for producing beta-galactosidase by liquid fermentation of Bacillus coagulans and the produced beta-galactosidase. Bacillus coagulans is specifically Bacillus coagulans (Bacillus coagulans) mutant BR-171, with the deposit number of CGMCC No. 14413. The strain is capable of producing beta-galactosidase stably and efficiently. The yield is more than 4000u / ml after submerged fermentation in a 50L fermentor and fed-batch fermentation for 84h. The beta-galactosidase produced by the strain has an optimum pH of 5.0, and still has an activity of more than 80% in the range of beta-galactosidase pH 2.5-8.5. The pH tolerance of galactosidase is suitable for most dairy products. The optimum temperatureof beta-galactosidase is 60 DEG C, and the activity of beta-galactosidase still reaches 88.4% after incubation at 65 DEG C for 2 h, which indicated that beta-galactosidase had significant heat resistance. It has high industrial value in dairy industry.

The invention discloses laccase-producing Coriolopsis gallica NCULAC F1. The laccase-producing Coriolopsis gallica is preserved in China Center for Type Culture Collection on June 17, 2021, the preservation number of the laccase-producing Coriolopsis gallica is CCTCC No.M2021731, and the preservation address of the laccase-producing Coriolopsis gallica is No.299, Bayi Road, Wuchang District, Wuhan, Hubei Province. The Coriolopsis gallica NCULAC F1 disclosed by the invention has the following characteristics that the laccase-producing activity is high, and produced laccase has good thermal stability and has relatively high tolerance to metal ions, organic solvents and the like. The Coriolopsis gallica NCULAC F1 is used as an enzyme-producing strain, and a proper amount of copper sulfate is added into a PDA liquid culture medium, so that the yield of the extracellular laccase with high activity expressed by the Coriolopsis gallica is greatly improved. The method for preparing a high-activity laccase crude solution from the Coriolopsis gallica NCULAC F1 by adding the copper sulfate has the characteristics of simplicity and convenience in operation, simple process, good repeatability and the like, and the prepared laccase solution is relatively high in activity and has good application potential and development and utilization prospects in the industrial field.

The invention discloses a biological system for efficiently detecting sweetness strength of a to-be-detected matter by taking yeast cell as host on the basis of combining sweetness receptor protein T1R2 / T1R3 with a detection gene path. The biological system comprises the sweetness receptor protein T1R2 / T1R3 subjected to modification and codon optimization, a modified Gpa1 protein adaptive to T1R2 / T1R3, a G protein coupling signal path for signal amplification without signal interference and an inducible detection gene path with fluorescent protein or enzyme as a reporter gene. After the to-be-detected matter is added into a culture environment and the sweet matter molecule is combined with the sweetness receptor, the host G protein coupling signal path is utilized to amplify the signal and activate a downstream detection path, the expression condition of the report gene is taken as signal output, and the sweetness strength of the sweet matter is represented by the signal output strength. The biological system disclosed by the invention has the advantages of simple culture condition, low cost, short period, wide detection scope, high repeatability, high stability and capability of realizing the efficient detection for the sweetness strength of different sweet matters.

The invention relates to the technical field of compound preparation, and specifically discloses a method for producing astaxanthin by fermenting Lactobacillus. The method is that by adding α-pinene and vitamin B12 to the fermentation medium, the lactobacillus that does not produce astaxanthin can be effectively induced to produce astaxanthin in large quantities, and the astaxanthin yield and biomass of the astaxanthin produced by it can be as high as about 600mg / L and 26g / L. The method of the present invention is a new way to produce natural astaxanthin by biosynthesis, and its excellent production performance makes it expected to be a kind of astaxanthin production that can replace Phaffia rhodozyma and Haematococcus pluvialis to produce astaxanthin method.

The invention belongs to the technical field of fermentation engineering, in particular to a Bacillus coagulans strain having a high beta-galactosidase yield and a liquid fermentation technique thereof. The strain is a Bacillus coagulans mutant strain BR-171 with an accession number of CGMCC No.14413. The strain can produce the beta-galactosidase stably in a high efficiency. Through deep fermentation in a fermentation tank having a volume of 50 L, and fermentation culture with material supplement for 84 h, the enzyme yield can be 4000 u / ml or above. The optimum pH of the beta-galactosidase produced by the strain is 5.0, and activity can still be 80% or more in a pH range of 2.5-8.5. The pH tolerance of the beta-galactosidase is suitable for most dairy products. The optimum temperature of the beta-galactosidase is 60 DEG C, and the residual activity of the beta-galactosidase can be 88.4 after incubation at 65 DEG C for 2 h, which indicates that the beta-galactosidase has significant heat resistance. The beta-galactosidase has high industrial value in dairy industry.

The invention belongs to the technical field of terpene compounds, and particularly relates to sesquiterpene cyclohexenone compounds, and a preparation method and application thereof. The sesquiterpene cyclohexenone compound provided by the invention has a novel framework structure, and diversity of sesquiterpene cyclohexanone compounds is enriched; and moreover, the sesquiterpene cyclohexanone compounds have anti-inflammatory and anti-tumor activity. According to the sesquiterpene cyclohexenone compounds prepared through microbial fermentation, the preparation method is short in period, mildin culture condition, few in byproducts, high in stereoselectivity, low in cost; industrialization can be realized easily; requirements of modern environmental protection and low carbon economy are met; and a new way can also be provided for production of sesquiterpene cyclohexanone compounds. The sesquiterpene cyclohexenone compound provided by the invention can be applied to preparation of anti-inflammatory and anti-tumor drugs, a new choice is provided for development of anti-inflammatory drugs.

The invention discloses a bacterial strain capable of producing alkali protease and an industrialized liquid fermentation method of the bacterial strain, and belongs to the field of bioengineering technology. The bacterial strain is mutant strain Ap180 of Bacillus alcalophilus, and the preservation number is CGMCC No.7545. The bacterial strain is obtained by repeat UV-induced mutation, nitrosoguanidine-induced mutation and selection of a wild bacterial strain obtained from saline lands. Characteristics of the bacterial strain are that: efficiency of alkali protease production is high, and stability is excellent. The Bacillus alcalophilus strain, which is capable of producing alkali protease and is suitable for industrialized production , is provided in the invention, and related fermentation mechanism is optimized. According to the fermentation mechanism, operation processes are simple, cultivating conditions are mild, fermenting enzyme activity is more than 80000u / ml, production cost of enzyme activity per unit is reduced, and the requirements of market are met. Applications of the alkali protease in fields such as washing agent, forage, and leather industry are more extensive.

The microbial pool provided by present invention is formed from Xanthomonas sp. dn 1 CGMCC No.1299, Flavobacterium sp.dn 2 CGMCC No. 1298, pseudomonas sp. dc 2 CGMCC No. 1297, Pseudomonas sp. dy1 CGMCC No. 1296 and Achromobacter sp. dy 3 CGMCC No. 1295. The above-mentioned microbial pool can be added into the soil polluted by oil, and can be used for degrading oil pollutant.

The invention provides bacterial strains for producing 5-hydroxyl-4-decalactone and application of the bacterial strains, belongs to the field of microorganisms, and discloses a bacterial strain capable of preparing 5-hydroxyl-4-decalactone by means of conversion, application of the bacterial strain to preparing the 5-hydroxyl-4-decalactone by means of microbial conversion and a method for applying the bacterial strain to preparing the 5-hydroxyl-4-decalactone. The bacterial strain can be used for efficiently preparing the 5-hydroxyl-4-decalactone by means of conversion by the aid of plant oil which is used as a substrate. The bacterial strain is preserved in the China Center for Type Culture Collection on May 19th, 2017, and a preservation number of the bacterial strain is CCTCC No.M2017274. The bacterial strain, the application and the method have the advantages that conditions for procedures for preparing the 5-hydroxyl-4-decalactone by means of conversion are mild, reaction can be quickly carried out, operation is simple, the method is safe as compared with existing chemical synthesis methods and the like, and environmental pollution can be prevented.

The invention provides a method of separating and preparing eburicoic acid compound by the fermentation liquid of long hair follicle fungus. A plate transgenic shaking flask liquid culture method is adopted. Firstly, glucose, peeled potatoes, MgSO4, KH2PO4, VB1 and water are mixed and stirred to prepare culture medium; then, culturing the culture medium to be fermented to obtain fermentation liquor; extracting the fermentation liquid to obtain an extract; drying mycelium and extracting with chloroform-methanol to obtain extract; combining the two extracts for the column chromatography separation and the chloroform-methanol gradient elution; a group of mixtures containing the compound are obtained at the chloroform-methanol elution part after the repeated column chromatography; petroleum ether-acetone elution is adopted to separate the mixtures to obtain a compound eburicoic acid pure product. The invention can solves the raw material source problem for developing the eburicoic acid into raw medicine of drugs for curing human leukemia. And the invention has the advantages of mild culture conditions, easy medium preparation, short fermentation tie, large yield, easy production in large scale, etc.

The invention belongs to the technical field of medicine, and in particular relates to a monoterpene indole compound and a preparation method and application thereof. The monoterpene indole compound (polonidine A) provided by the present invention has good inhibitory activity on liver cancer cell MHCC97H, breast cancer cell BT549, lung cancer cell H1299, colon cancer cell SW620, glioma T98G and lung cancer cell A549, its IC 50 The values ​​were 7.08 μg / mL, 6.05 μg / mL, 20.39 μg / mL, 7.64 μg / mL, 17.97 μg / mL and 15.96 μg / mL, respectively. The polonidine A provided by the invention provides a new approach for the development of antitumor drugs. The invention prepares monoterpene indole compounds through microbial fermentation, has short preparation period, mild culture conditions, few by-products, low cost, and is easy to realize industrialization.

The invention relates to a method for separating and preparing compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) with the fermentation broth of Polyporus picipes. The method adopting a plate-to-bottle liquid culture method includes the following steps: a peeled potato, glucose and distilled water, the pH of which is about 7.2, are first sterilized and prepared into culture medium; the culture medium is then fermented into fermentation broth, the fermentation broth is extracted with ethyl acetate to obtain extract, and the extract is separated by column chromatography, and is eluted at a gradient by a chloroform-methanol system; and after separation by multiple times of normal-phase silica gel column chromatography, a reversed-phase C18 column, SephadexLH-20 gel column chromatography and recrystallization, the pure compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) is obtained. The invention can develop the compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) into the technical product of pathogenic fungus-resistant antibiotic, solves the problem of material resources for the 2,4-dyhydroxyl-5-methyl-acetophenone, and has the advantages of mild culture conditions, simple and easy culture medium preparation, short fermentation time and the like.

The invention discloses a preparation method of a squalene synthetase inhibitor CJ-13982. The compound CJ-13982 is separated and prepared from a fermentation culture of nigrospora oryzae A8 which has a collection number of CCTCC NO. M 2011412. The invention provides a method for preparing the compound CJ-13982 with the squalene synthetase inhibiting effect from the fermentation culture of a plant endophytic fungi nigrospora oryzae A8. The method is advantaged in mild culturing condition, simple and feasible culturing medium preparation, simple separation and purification steps, and high yield. With the method, large-scale production can be realized in a manual controlling condition. With the method, a problem of raw material required by compound CJ-13982 researches or applications can easily be solved, and a novel approach is provided for the preparation of the compound CJ-13982.

The invention discloses an edible soybean sprout rich in trace elements of zinc, cobalt and selenium and a cultivation method thereof; the total zinc content of the soybean sprout is 5.76-8.47mg / kg, the total cobalt content is 31.24-104.67μg / kg, the total selenium The content of organic zinc is 12.60~80.40μg / kg, the content of organic zinc is 1.57~4.65mg / kg, the content of organic cobalt is 7.05~43.39μg / kg, and the content of organic selenium is 3.11~66.14μg / kg; , Co and Se are recommended intake standards in the Chinese Dietary Guidelines, and there are no potential safety hazards. The cultivation method of the present invention can guide the batch production of edible soybean sprouts rich in zinc, cobalt and selenium, has short growth period, high organic conversion rate of trace elements, moderate enrichment amount, no hidden danger in production and consumption, and low production cost.

The invention relates to an application method for potash fertilizer. The application method for the potash fertilizer is characterized by comprising the following steps: sampling to-be-fertilized land so as to obtain a soil sample; preparing a liquid soil medium from the soil sample; culturing silicate bacteria with the liquid soil medium so as to obtain a culture solution; measuring the content of rapidly available potassium in the culture solution; and applying the potash fertilizer and silicate bacteria fertilizer according to the content of rapidly available potassium and demands of the to-be-fertilized land for potassium. The application method for the potash fertilizer is beneficial for reasonable fertilization.

The invention belongs to the technical field of terpene compounds, and particularly relates to sesquiterpene cyclohexenone compounds, and a preparation method and application thereof. The sesquiterpene cyclohexenone compound provided by the invention has a novel framework structure, and diversity of sesquiterpene cyclohexanone compounds is enriched; and moreover, the sesquiterpene cyclohexanone compounds have anti-inflammatory and anti-tumor activity. According to the sesquiterpene cyclohexenone compounds prepared through microbial fermentation, the preparation method is short in period, mildin culture condition, few in byproducts, high in stereoselectivity, low in cost; industrialization can be realized easily; requirements of modern environmental protection and low carbon economy are met; and a new way can also be provided for production of sesquiterpene cyclohexanone compounds. The sesquiterpene cyclohexenone compound provided by the invention can be applied to preparation of anti-inflammatory and anti-tumor drugs, a new choice is provided for development of anti-inflammatory drugs.

The invention relates to sugarcane and provides a method for preparing mixed liquor of glucose and fructose from bagasse. The accession number of the bacterial strain Bacillus aryabhattai GZ03 is CCTCC No: M2013521. The method for preparing mixed liquor of glucose and fructose from bagasse comprises the following steps: 1) activating the bacterial strain Bacillus aryabhattai GZ03 with an activation medium plate, inoculating the bacterial strain in a seed medium and carrying out shake cultivation so as to obtain a seed liquid; 2) inoculating the seed liquid obtained in the step 1) into a sugar-producing medium, continuing shake fermentation cultivation so as to obtain fermentation broth, carrying out centrifugation and then collecting a supernatant; and 3) subjecting the supernatant collected in the step 2) to filtering, wherein the obtained filtrate is the mixed liquor of glucose and fructose. The mixed liquor of glucose and fructose can be used in preparation of food additives and health product additives; the food additives and health product additives may be sweeteners which are especially added into beverages.

A process for synthesizing the beta-dihydrogarofuran sesquiterpene polyol ester compounds includes such steps as inoculating Agrobacterium C58 to bitterbark vine, taking crown atissue, culturing and extracting target product. Its advantages are less investment, low cost and short period.

The present invention relates to a method for synthesizing secoiridoid glycoside compound, belonging to the field of biolgoical conversion synthesis technology of organic compound. Said method includes four steps of preparation of culture medium, induction of explant, tissue (cell) culture and extraction of target molecule. Said invention only can utilize solid culture medium to make culture and extraction, also can utilize solid culture medium to make culture firstly, then utilize liquid culture medium to make culture and extraction. Said method and process are simple, and high in yield.

The invention relates to the technical field of microbes, and provides Penicillium roqueforti with a preservation number of CGMCC No.14140. The penicillium loudi provided by the invention is derived from the root of Aconiti japonica, and is an endophytic fungus of Aconiti japonica. The metabolite of Penicillium loudii in the present invention contains a sesquiterpene compound peniroquesine A with a novel 5 / 6 / 5 / 6 / 5 pentacyclic new skeleton structure, and has significant anti-inflammatory activity. The Penicillium loudii provided by the invention can simply and efficiently prepare the sesquiterpene compound peniroquesine A, which is used for preparing anti-inflammatory drugs. The preparation of sesquiterpenoids by microbial fermentation has the advantages of short cycle, mild culture conditions, few by-products, strong stereoselectivity and low cost.

The invention belongs to the technical field of fermentation engineering, in particular to a method for producing β-galactosidase by liquid fermentation of Bacillus coagulans and the produced β-galactosidase. The Bacillus coagulans is specifically a Bacillus coagulans mutant strain BR-171, and the preservation number is CGMCC No.14413. The strain can produce β-galactosidase stably and efficiently. Through submerged fermentation in a 50L fermenter, and fed-batch fermentation for 84 hours, the enzyme production can reach more than 4000u / ml. The optimal reaction pH of the β-galactosidase produced by the strain is 5.0, and still has more than 80% activity in the pH range of β-galactosidase pH 2.5 to 8.5, and the pH tolerance range of the enzyme is suitable for large Some dairy products; the optimum temperature for β-galactosidase is 60°C, and the remaining enzyme activity still reaches 88.4% after being incubated at 65°C for 2 hours, with remarkable heat resistance; it has high industrial value in the dairy industry .

The invention discloses a method for preparing diaporthein B and application of the diaporthein B to the preparation of antitumor medicaments. In the method, the diaporthein B with antitumor activity is separated from a liquid fermentation culture of a marine fungus, namely Eutypella scoparia FS26. The method has the advantages that culture conditions are mild, a culture medium is easy to prepare, fermentation time is short, separation and purification steps are simple, the mass production can be realized under manual control conditions and the like, and the problem of medicament sources can be solved more conveniently. Through the experiment, IC50 values of the diaporthein B for a glioma cell SF-268, a breast cancer cell MCF-7 and a large-cell lung cancer cell NCI-H460 are 92mu M, 44mu M and 9.9mu M respectively, the diaporthein B has remarkable antitumor activity, and IC50 values of a positive control, namely cis-platinum for the three tumor cell strains are 4.6mu M, 4.1mu M and 3.0mu M respectively. Therefore, a candidate compound is provided for researching and developing novel antitumor medicaments, and a scientific evidence is provided for developing and utilizing natural active substances which are derived from marine microorganisms.

The invention discloses a method for inducing sporulation of hyphosporium fungus, which comprises the following steps: (1) take circular filter paper, evenly cut out more than 4 filter paper holes, lay it flat on a petri dish, add appropriate amount of distilled water to soak, Remove the filter paper after autoclaving. (2) Prepare and sterilize the agar medium, and then add it to the sterilized petri dish, the amount of each petri dish is 15-20ml, and make the agar culture plate after the agar medium is solidified. (3) Spread the sterilized filter paper on the surface of the agar culture plate. (4) Pick an appropriate amount of non-sporogenous hyphomycete fungi and inoculate them on the improved agar culture plate at the center of each filter paper hole. (5) Cover and seal the petri dish, and incubate at room temperature under natural light for 10 to 30 days. The method of the invention has simple operation, mild culture conditions, remarkable effect of inducing sporulation of hyphosporium fungi, is effective for most hyphosporium fungi, conidia are evenly distributed on filter paper, and is convenient for observing sporulation phenotypes.

The invention relates to a method for preparing a compound of 2, 4-dihydroxy-5-methyl-hypnone by separating the fermentation liquid of Polyporus picipes. In the method, a method of plate-to-flask liquid culture is adopted; firstly, peeled potato, glucose and distilled water with the pH of 7.2 approximately are sterilized and made into a culture medium; then the culture medium is cultured and fermented to obtain the fermentation liquid; the fermentation liquid is extracted by ethyl acetate to obtain extractum; the extractum is separated by column chromatography and is eluted by a chloroform-methanol system in a gradient way; and the pure compound of the 2, 4-dihydroxy-5-methyl-hypnone (II) is obtained after several times of silica gel column chromatography, reverse C18 column and SephadexLH-20 gel column chromatography and separation by a recrystallization method. The invention can develop the 2, 4-dihydroxy-5-methyl-hypnone (II) into the original drug of antipathogen fungi antibiotic and solves the problem of the raw material source of the 2, 4-dihydroxy-5-methyl-hypnone, and the invention has the advantages of mild culture condition, being simple and easy for confecting the culture medium, and short fermentation time, etc.