605results about How to "Simplify detection steps" patented technology

The invention discloses a preparation method of an electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and an application method of the electrochemical three-dimensional microfluidic paper chip to a field test. According to the invention, hydrophobic wax patterns are printed on three pieces of common filter paper with the A4 size respectively in batches by a full-printing-level adhesion piling mode. The preparation process comprises the following steps: printing the hydrophobic wax patterns in batches; melting wax and forming; screen-printing an array working electrode, a reference electrode and a counter electrode on the filter paper in batches; printing horse radish peroxidase and oxidase on the surface, without the working electrode, of the filter paper sequentially in batches; cutting the electrochemical three-dimensional microfluidic paper chip; preparing a double-sided adhesive tape sheet; adhering the filter sheets through the double-sided adhesive tape sheet; and preparing an electrochemical three-dimensional microfluidic paper chip clip. A method for performing field test on the electrochemical three-dimensional microfluidic paper chip comprises the following steps: clamping the paper chip by using the electrochemical three-dimensional microfluidic paper chip clip; inserting into six copper finger slots; and connecting an electrochemical work station through a multiplexer.

The invention belongs to the technical field of neoplastic disease diagnosis, and discloses a kit for early diagnosis of neoplastic disease, a method and applications of the kit. The kit includes a specific capturing and detecting system for a tumor exosome and a molecular beacon fluorescent detection system. The specific capturing and detecting system for the tumor exosome includes a solution ofa magnetic gold nanosphere composite modified by a tumor exosome specific ligand and adopting ferric ferrous oxide as a core, and a gold nanocage solution modified by the exosome specific ligand. Themolecular beacon fluorescent detection system includes a molecular beacon probe provided with a fluorescent group, fluorescent amplification substrate hybrid chains provided with fluorescent groups and a buffer liquid. The kit and the method combine tumor exosome specific capturing and analysis and a molecular beacon fluorescent detection method, and greatly increases accuracy and reliability of early diagnosis of tumor.

The present invention discloses a preparation method of a simple, low-cost, multi-channel microfluidic chemiluminescent paper chip and an on-site detection method by using the same. By using a full print mode, a plurality of multi-channel microfluidic chemiluminescent paper chips are printed out on a common A4-size filter paper in batch. The printing process includes: printing hydrophobic wax patterns in batch; melting wax for molding; printing chemiluminescent reagent ink patterns in batch; printing oxidase ink pattern in batch; cutting the microfluidic chemiluminescent paper chips into certain shape; and performing plastic package to the prepared microfluidic chemiluminescent paper chips. An on-site detection method by using the microfluidic chemiluminescent paper chip comprises the steps of: placing the plastic packaged microfluidic chemiluminescent paper chip into the cartridge of a handheld luminescent detector; dropwise adding a sample solution into the sample region; then covering the cartridge with a lid, and starting the test. The existence and contents of glucose, polyphenols, xanthine, cholesterol, uric acid, and hemoglobin are sequentially determined via the size of six chemiluminescence peaks sequentially appearing.

The invention discloses a system and a method of detecting a pulmonary nodule. The system comprises an image preprocessing module, a three-dimensional RPN network model and a three-dimensional capsulenetwork model, wherein the image preprocessing module is used for preprocessing an original three-dimensional CT image to acquire a standard CT image; the three-dimensional RPN network model is usedfor detecting a first candidate pulmonary nodule from the standard CT image outputted from the image preprocessing module; and the three-dimensional capsule network model is used for carrying out false positive pulmonary nodule screening on the first candidate pulmonary nodule outputted by the three-dimensional RPN network model to acquire a pulmonary nodule detection result. According to the technical scheme provided in the invention, while the false positive pulmonary nodule detection rate is ensured to be lower, a high recall ratio is ensured to be realized for the pulmonary nodule, and thedetection method is simple and the detection speed is quick.

The invention discloses a quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting multicomponent food additives simultaneously and a method for detecting the food additives by the quantum dot molecular imprinting microsphere quartz fluorescent sensor. A method for preparing a quantum dot molecular imprinting microsphere comb quartz plate comprises the following steps of: selecting functional monomers corresponding to the food additives; preparing quantum dots and preparing quantum dot molecular imprinting microspheres according to the literature; and modifying thesurfaces of different probes of the comb quartz plate with the imprinting microspheres of different food additives by utilizing layer-upon-layer accumulated surface modification technology. The method for detecting the trace multicomponent food additives simultaneously (which is shown as the figure) comprises the following steps of: immersing the modified quartz plate into simply-pulpified food solution, arranging the quartz plate on a sealing quartz vessel, and detecting the food additives in a sample. The quantum dot molecular imprinting microsphere quartz fluorescent sensor has high specificity and sensitivity, short detection time and low cost; and in a method for detecting pesticide residues by fluorescent light, the operation is simple and quick, and reactions and results are completed and recorded automatically by instruments.

The invention discloses a tea quality rapid detection apparatus and a detection method based on characteristic fragrance, which is characterized in that the characteristic information of the tea fragrance is analyzed and treated, and the quantitative analysis and qualitative analysis are rapidly and accurately carried out on the tea quality. According to the invention, a gas-sensitive sensor array is used for analyzing the tea sample smell aggregation gas in the gas-sensitive sensing device reaction chamber, by comparing the data in a computer and a database, the quality grade information of the detected sample and the characteristic fragrance content information of the detected sample can be output according to the user requirement, the characteristic information of the tea fragrance is analyzed and treated, and the quantitative analysis and qualitative analysis are rapidly and accurately carried out on the tea quality. the detection step is greatly simplified, the pretreatment of the detected sample is not required, the artificial factor interference brought by sensory evaluation can be avoided, the determination result is more objective and accurate, and the invention provides the novel nondestructive test apparatus and the detection method for detecting the tea quality.

The invention provides a rolling circle amplification based miRNA (micro ribonucleic acid) assay probe, an assay method and a kit. The probe comprises a hairpin probe and a ring probe, wherein the hairpin probe comprises a 5' terminal side chain (1), a loop (2) and a 3' terminal side chain (3), the 5' terminal side chain (1), the loop (2) and the 3' terminal side chain (3) are single nucleotides; the 5' terminal side chain (1) and the loop (2) are provided with a nucleotide sequence supplementary with miRNA to be assayed; partial nucleotide sequences of the 5' terminal side chain (1) and the 3' terminal side chain (3) are supplementary; the 3' terminal side chain (3) is provided with a nucleotide sequence supplementary with partial nucleotide sequence of the ring probe. The miRNA assay method can distinguish miRNA to be assayed, miRNA similar to a target sequence and target miRNA precursor; extraction and purification of miRNA is not needed when a blood sample is assayed.

The invention relates to a mechanical-vision-based injection molding machine mold protecting system, which comprises a master control board using an embedded chip, a camera, a near infrared light source and a magnetic fixed seat, wherein the master control board is connected with an injection molding machine through an input / output (I / O) signal interface and connected with the camera through the camera interface; the camera and the near infrared light source are arranged on the magnetic fixed seat; and the magnetic fixed seat is arranged around the injection molding machine, so that the camera can acquire an image in the mold after the injection molding machine mold is opened under the near infrared light source. The invention also relates to a mechanical-vision-based injection molding machine mold protecting method, which comprises the following steps of: judging whether a product is qualified by using the camera, shooting the mold again after blanking, judging whether the blanking is successful, and giving a detection result signal. The blanking condition is judged directly through the mold image acquired by the camera, and the un-falling residual product in the mold is intelligently analyzed, so that the method is quite reliable and the mold is safer.

The invention provides an online partial discharge detection signal recognition method of a cable. The method comprises the following steps: a partial discharge time domain waveform of a known source is acquired; a partial discharge waveform time domain waveform sample library is established; a wavelet packet is used to denoise each partial discharge time domain waveform in the sample library; a self-adaptive wavelet neural network model with a preset number of layers is constructed; according to each denoised partial discharge time domain waveform, a PSO algorithm is used to train the constructed self-adaptive wavelet neural network model; a BP algorithm is then used to train the trained self-adaptive wavelet neural network model for a second training so as to get a well-trained wavelet neural network; and the partial discharge signal of a to-be-recognized source is received, the well-trained wavelet neural network is inputted for recognition, and the source of the to-be-recognized partial discharge signal is obtained. The method of the invention is fast in recognition speed and high in recognition precision.

The invention discloses a PIK3CA gene mutation site detection probe, a detection liquid phase chip and a detection method thereof. The PIK3CA gene mutation detection liquid phase chip mainly comprises a microball covered with a probe and a primer for amplifying a target sequence carrying a 9th exon and / or a 20th exon. The PIK3CA gene mutation detection liquid phase chip and the detection method can help simultaneously detect the sites with relatively higher frequency of the PIK3CA gene mutation, and can separately detect the 9th exon and the 20th exon or detect the 9th exon and the 20th exon at the same time, and the detection has uniform reaction condition, good specificity of detection results, high sensitivity, good accuracy up to 90% and short detection time.

A high-flux detection system and the electrochemistry immune analytical method to detect many analytes at the same time has provided in the invention. It uses the functional chitosan membrane modified by the medium to fix the antigen molecule combining with the competitive immune analytical method, so the antigen in electrode surface competes with the antigen in sample to combine with the enzyme labeled antibody in warm cultivating solution to link the part antibody into the electrode surface, which catalyses the reaction between the H2O2 and the Toluidine Blue fixed in the electrode surface to get the detected antigen content in solution indirectly by the current catalyzed by the H2O2 oxidation. So the electrical medium is fixed in the electrode surface to avoid the signal cross generated by the diffusion which is the immune sensor array without disturbance. Combining with the flowing injection technology, it reaches the high-flux detection output. The sensor array is simple and cost little, also it has the short analyzing time and high sensitivity, so it is important to the life analytical chemistry, the immune science and the clinical medicine.

The invention discloses an online mold monitoring and protecting system and an online mold monitoring and protecting method based on contour matching. The online monitoring and protecting method comprises the following steps: a light source of near-infrared mixed visible light generates near-infrared mixed visible light on a mechanical jig; an image acquisition device acquires an image of the mechanical jig, and sends the image to an image processing device; the image processing device receives image information of the image acquisition device, and a near-infrared fusion visible light image of the mechanical jig is acquired and is matched with the contour of a standard image by adopting an image processing algorithm of contour matching; a control command is generated according to the matching result; a jig control system receives the control command of the image processing device so as to control the movement of the mechanical jig. According to the invention, the information that whether a moving mold and a fixed mold are good or not is acquired rapidly and accurately, and the defects of an existing mold online monitoring and protecting technique are remedied.

The invention discloses a preparation method of an SERS substrate of Au@Ag nanoparticles. According to the method, gold nanoparticles are prepared by reducing chloroauric acid with sodium citrate, thegold nanoparticles are modified with 4-sulfydryl aniline to link silver ions, and a certain proportion of ascorbic acid and sodium hydroxide are added to reduce a silver shell. Meanwhile, the form size of the core-shell structure is controllable, plasma resonance is realized through high coupling of the structure, and the so-called hotspots are formed in the core-shell structure, so that 4-sulfydryl aniline serving as an internal standard molecule has very high Raman signals. The Au@Ag core-shell structure is modified with 4-sulfydryl phenylboronic acid to capture glucose molecules, so that qualitative and quantitative detection of glucose is realized. The method has the advantages as follows: the internal standard substance is used for signal comparison, interference of complex substances in samples (such as urine) can be avoided, the detection sensitivity is improved, the preparation cost is low, and qualitative and quantitative detection of glucose is really realized.

The embodiment of the invention discloses a performance detection method of a smart contract on a block chain. The method includes: generating a detection page according to a json file output by the compiled smart contract; acquiring detection request parameters of a user on the detection page; and entering a corresponding detection process according to the detection request parameters, and returning a detection result after the detection process ends. According to the embodiment of the invention, detection pages can be automatically generated for various smart contracts, detection request parameters input by the user can be acquired on the detection pages, fully automatic detection can be carried out, detection steps are simple and convenient, and detection efficiency is improved.

The invention relates to a nucleic acid aptamer electrochemical sensor based on metal organic framework materials (MOFs) as signal probes. The senor is used for detection of beta-amyloid (A beta) oligomers and belongs to the technical field of functional nanocomposite materials and biosensor detection. AuNFs-modified glassy carbon electrodes immobilize a first layer of aptamers, the A beta oligomer is further identified and through combination with second layer aptamer-modified gold nanoparticle-loaded Cu-MOFs (AuNPs / Cu-MOFs), the sandwich electrochemical sensor for detection of the A beta oligomer is formed. AuNPs / Cu-MOFs as a redox medium can directly detect a Cu<2+> signal and electrochemically active molecule labeling is avoided so that the detection processes are greatly simplified and the sensitivity of detection is improved.

The invention discloses preparation and application of a simple high-throughput microfluidic chemiluminescence paper chip. A plurality of microfluidic chemiluminescence paper chips are printed in batches on B5 plain filter paper by using a full print mode. The printing process comprises batch printing of hydrophobic wax patterns, melted wax molding, bath printing of chemiluminescence reagent ink patterns, batch printing of oxidase ink patterns, bath printing of flow rate adjusting hydrophobic wax patterns, laser cutting of microfluidic chemiluminescence paper chips, and plastic sealing treatment of the prepared microfluidic chemiluminescence paper chips. A field test method of the microfluidic chemiluminescence paper chip comprises the following steps: placing the plastic-sealed microfluidic chemiluminescence paper chips in a cassette of a palm light emitting testing instrument; dropwisely adding a sample solution to a sampling area; closing a cassette cover, and starting testing. The existence and contents of 32 kinds of tested components are determined by the sizes of 32 successively appearing chemiluminescence peak values.

The invention provides a fast multiplex PCR identification diagnosis detection method of invasive fungus infection based on cfDNA (cell free DNA). The method can be used for identifying the invasive infection caused by clinic common and high-incidence Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei, Candida glabrata and Aspergillus fumigtus. An amplification primer is designed according to characteristic genome segments of each fungus category and species; a detection fluorescence probe of a strain can be distinguished according to the amplification segment design; the real time PCR can be performed on a sample to be tested; high sensitivity of nested PCR and high specificity and multi-target performance advantages of multiple fluorescent hybrid probe PCR are integrated for identifying the fungus strain. The invention also provides a PCR diagnosis kit for the invasive fungus infection and application thereof.

The invention discloses an RPA primer for detecting a duck-derived component, a kit and a detection method. The RPA primer comprises a forward primer RPA-duck-F and a backward primer RPA-duck-R, and the forward primer RPA-duck-F and the backward primer RPA-duck-R respectively have the specific sequences as follows: 5'-TGAATCCTGTTGCCGGTCTTGCGATGATTATC-3' and 5'-TCTATCGCGTATTAAATGTATAATTGCGGGAC-3'. By the use of the RPA primer, whether a detected product contains the duck-derived component can be accurately, quickly and easily detected, and the RPA primer has the characteristics of high sensitivity, high amplification speed and high detection result intuitiveness and can realize quick and simple detection for animal-origin meat and meat products at normal temperature and meet the requirements of the government for market supervision and routine monitoring.

To obtain a power conversion device that makes it possible to: both lengthen phase-current detection periods and simplify detection procedures; and control a carrier frequency so as to improve device performance. A power-supply shunt resistance (5) is provided between the load-voltage side of a DC power supply (1) and an inverter (2), and a lower-arm shunt resistance (6a through 6c) for each phase is provided between a lower-arm switching element (3d through 3f) for that phase and the power-supply shunt resistance (5). For each phase, a lower-arm voltage is detected, said lower-arm voltage being the voltage between the load-voltage side of the DC power supply (1) and the point where the lower-arm switching element (3d through 3f) for that phase connects to the lower-arm shunt resistance (6a through 6c) for that phase. The amount of current flowing through a load device (9) in each phase is computed on the basis of the detected lower-arm voltages, and the carrier frequency of a carrier signal, which serves as a base frequency for drive signals, is controlled in accordance with changes in a specific control parameter (A).

The invention provides an obstacle detection method and device, equipment and a computer storage medium. The obstacle detection method comprises the steps: data collected by a plurality of sensors atthe same moment are obtained, wherein the multiple sensors are mounted on a vehicle; the data collected at the same moment are combined, and a data set corresponding to the moment is obtained; and obstacle detection is conducted according to the data set, and obstacle information is obtained. The financial cost can be reduced, the information amount of the data can be enriched, the obstacle detection step can be simplified, the problem that the data in different formats are fused unsuccessfully is avoided, and thus safety of vehicle traveling is improved.

The invention discloses a method for detecting the diameter of a rotary jet grouting pile. Whether the upper and lower parts of a rotary jet grouting pile to be detected are consistent in diameter is judged by comparing the upper and lower points of the pile in such aspects as sonic time, amplitude of first wave and curve integrity, the detection result is processed to get the diameters at different points, and the obtained diameters of the rotary jet grouting pile are verified by use of a calculation formula. The detection result is more accurate and reliable, and the construction parameters can be changed in a timely manner in the process of construction to make the upper and lower parts of the rotary jet grouting pile consistent in diameter. Through the detection method, quick nondestructive detection of the diameter of a rotary jet grouting pile is realized, money and cost are saved, and deviation can be corrected in time according to the detection result to ensure the engineering quality.

The invention discloses a method for measuring nano-gold mimetic peroxidase based urease and an inhibitor thereof. The method is characterized by comprising the following steps: coupling a urease catalysis urea reaction system with a nano-gold mimetic peroxidase-hydrogen peroxide-3,3',5,5'-tetramethylbenzidine dihydrochloride catalytic chromogenic reaction system, and combining with the changes of solution colors and ultraviolet absorption spectrum characteristics to directly measure the content of urease and the inhibitor thereof, wherein in a range of 1.8-90U / L, A450 and the urease concentration have a linear relationship, and the limit of detection is 1.8U / L; and by virtue of software fitting, IC50 of obtained acetohydroxamic acid of the urease inhibitor is 0.05mmoL / L. The method disclosed by the invention is simple and convenient to operate and high in sensitivity, and can be applied to the measurement of the content of urease and the inhibitor thereof in environment and life science systems as an analysis method.

A method for using gold nanoparticles to detect single-stranded DNA base mutation relates to a detection of gene mutation and is provided. Colloidal gold is prepared to react with the single-stranded DNA to form the colloidal gold and a single-stranded DNA composite system; hydrochloride is added and the color of the composite system turns from red to purple or blue. Whether the single-stranded DNA mutates or not is judged by making use of the sequence differences between a wild-type single-stranded DNA and a mutant single-stranded DNA and through the final color changes of the composite system or the differences of the positions of the maximum absorption peak red shift of ultraviolet-visible absorption spectra. Both the single-stranded DNA and the gold nanoparticles can detect single base mutation without any decoration or special device; the lowest detection limit is 10 fmol and detection results can be given within 30 minutes. The method has the advantages of convenience, rapidness, low cost, high sensitivity, high specificity, strong practicality and so on and has big clinical application potential.

The invention discloses a method for simultaneously determining multiple plant growth regulators in exported vegetables. The method comprises the following steps: extraction, purification, concentration, ultrahigh-performance liquid chromatography-triple quadrupole / composite linear ion hydrazine mass spectrometer (UHPLC-QTRAP) determination and the like. The method can simultaneously determine the 17 plant growth regulators, including gibberellin, indolyl-3-butyric acid, p-chlorophenoxyacetic acid, abamectin, diethyl aminoethyl hexanoate, atrazine, simazine, 6-benzyl aminopurine, 2,4-dichlorphenoxyacetic acid, forchlorfenuron, mepiquat chloride, chlormequat chloride, paclobutrazol, uniconazole, abscisic acid, thidiazuron and p-fluorophenoxyacetic acid. The method disclosed by the invention has the advantages of simple steps, short time consumption, high accuracy and high precision.

The invention provides a dust concentration calibrating system and method suitable for a photoelectric method dust concentration detector. The system comprises a power device, a dust prefabricating feeding device, a temperature transmitter, a pressure transmitter and a detector calibrating node device. The power device is used for providing power for circulation of gas containing dust. The dust prefabricating feeding device is used for preparing a dust sample with the known mass into dust gas with the prefabricating concentration, feeding the dust gas into the system and compensating for leakage gas. The temperature transmitter is used for measuring the temperature of the dust gas in the dust concentration calibrating system. The pressure transmitter is used for measuring the pressure of the dust gas in the dust concentration calibrating system. The detector calibrating node device is used for installing the dust concentration detector and measuring the leakage amount of the gas containing dust when the dust concentration detector is replaced. According to the dust concentration calibrating system and method suitable for the photoelectric method dust concentration detector, the calibrating steps of a traditional dust concentration detector are simplified, the detection efficiency is improved, and a precise calibrating result is obtained.

The invention relates to the technical field of storage battery, and more specifically, relates to a main fuse fusing and open-circuit detection method and apparatus for a storage battery pack. The main fuse fusing and open-circuit detection method and apparatus for a storage battery pack can monitor the storage battery state in a transformer station, and can quickly detect and determine the result and generate an alarm signal when the storage battery is separated from a bus, thus being convenient for maintenance for the staff, and guaranteeing safety of the standby power supply of the transformer station.

The invention discloses a control method of a foldable arm rest. The control method includes a detection step: detecting whether each arm section is in a completely closed state or not, a calculation step: ensuring that the arm section is in a completely folding state when the arm section is not in a completely closed state, obtaining the folding state of each arm section, working out the maximum distance L between the arm rest portion which is between the predetermined arm section and the tail end of the folding arm rest and a hinge point of the root end of the predetermined arm section according to the folding state of each arm section, and working out the allowable maximum angular velocity w when the predetermined arm section moves according to a predetermined linear velocity V and the maximum distance L, and a control step: controlling an actuating part which drives the predetermined arm section, and enabling the predetermined arm section to move at an angular velocity which is less than or equal to the maximum angular velocity w. The invention correspondingly discloses a control system of the foldable arm rest and engineering machinery. The control method and the control system has the advantages of being capable of improving folding speed of the foldable arm rest and working efficiency, easy to control, low in cost and good in stability.

The invention discloses a fluorescence nanocrystal quartz fluorescent sensor for molecular identification-based multicomponent simultaneous saccharide detection and a method for detecting saccharides by using the same. The invention provides a preparation method of a molecular identification-based fluorescence nanocrystal comb quartz plate, which comprises the following steps: selecting an identifier corresponding to the saccharides; preparing a fluorescence nanocrystal, and carrying out surface modification on the fluorescence nanocrystal according to the literature; and modifying the modified fluorescence nanocrystal onto surfaces of different probes of the comb quartz plate by using layer-by-layer accumulation surface modification technique. The invention also provides a method for multicomponent simultaneous saccharide detection, which comprises the following steps: immersing the modified quartz plate into a food sample solution which is simply pulpified, installing the quartz plate onto a sealed silica dish, and detecting the saccharides in the sample. The invention has the advantages of strong specificity, high sensitivity, short detection time and low cost. The saccharide fluorescence detection method has a quick and simple operation process, and the reaction and the result are automatically completed and recorded by instruments.

The invention discloses a biological target molecule detection method based on multivalent capture and output signal amplification, and belongs to the technical field of biological detection. The detection method comprises the following steps: adding an identification probe into a solution to be detected, identifying and combining targeted biological target molecules, releasing an initiating sequence, allowing the initiating sequence to open hairpin probes to generate a hybridization chain reaction, adding an auxiliary probe, sealing hairpin probes which do not participate in the reaction, placing a carrier which is self-assembled with a capture probe on a gold interface in the reaction solution, capturing a hybridization chain reaction product onto a gold sheet, and finally analyzing thesignal mark change on the gold interface to further calculate the content of the targeted biological target molecules in the sample to be detected. The method forms a plurality of hybridization chainreaction products are formed through the hybridization chain reaction, and then utilizes interface multivalent capture and signal output amplification, so that the detection of target molecules is realized; and under the help of the auxiliary probe, the efficiency of capturing hybridization chain reaction products by the sensing interface is improved, and the detection sensitivity is improved.