50 results about "Chelex 100" patented technology

The invention discloses a whole blood genomic DNA high-flux plate type extracting kit and an extracting method, and relates to the technical field of biology. The whole blood genomic DNA high-flux plate type extracting kit comprises a lysate 1, a lysate 2, a combination liquid, and magnetic beads, wherein the lysate 1 comprises the following components: 2 to 8 mol/L guanidine hydrochloride, 1 to 10 percent of triton-100 (V/V), 1 to 10 percent of Tween-20 (V/V), 1 to 10 percent of chelex-100 (m/V), 2 to 10 mmol/L ethylene diamine tetraacetic acid and 20 to 100 mmol/L tromethamine, wherein the pH is regulated to be 4.5 by hydrochloric acid, and the solvent is deionized water; the lysate 2 comprises the following components: 20 mg/ml protease K and 50 mmol/L trimethyl aminomethane, wherein the pH is regulated to be 8.0 by the hydrochloric acid; the combination liquid comprises the following components: 1 to 3 mol/L sodium chloride and 40 to 90 percent of isopropyl alcohol (V/V), wherein the solvent is the deionized water. According to the whole blood genomic DNA high-flux plate type extracting kit and the extracting method, the purpose of extracting 96 samples simultaneously within 50 minutes can be achieved without centrifuging or matching an automatic instrument.