33 results about "Environmental studies" patented technology

The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and / or treatment of disease conditions.

The invention can be used in biology and for environmental studies using fluorimeters. The inventive method consists in producing exiting light pulses having equal amplitude and modifiable duration, in measuring fluorescent chlorophyll characteristics at a constant background illumination simulating the irradiation intensity of an object during studies in the natural conditions and after the adaption thereof in the dark and in determining the state of a photosynthetic apparatus according to the entirety of fluorescent intensity values. The inventive device comprises the even number of measuring light sources, a current stabiliser of the light sources, the outputs of which are connected to electric inputs of the light sources, the input of which is connected to a control unit and a natural irradiation sensor is connected to the current stabiliser of the light sources through said control unit. A measurement chamber comprises the even number of light sources which are arranged by pairs diametrically oppositely to each other in one plane which is perpendicular to the axis of the body.

The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and / or treatment of disease conditions.

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and / or treatment of disease conditions.

The invention relates to en ecological element processing method and system for a complex ecological coastal zone. The method is characterized by obtaining image data of a target complex ecological coastal zone through multi-source remote sensing and processing the image data; then, carrying out coastline classified extraction according to different types of coasts, and extracting coverage comprising vegetation, rivers and artificial coastline surfaces; and finally, according to land utilization degree grade proposed based on ecological perspective and each coverage type ecosystem service variation contribution rate, defining elements having positive ecological contribution and positive ecosystem service values as positive ecological elements and defining elements having negative ecological contribution and negative ecosystem service values as negative ecological elements to construct a coastal zone ecological element classification system. According to the land utilization degree grade proposed based on ecological perspective and each coverage type ecosystem service variation contribution rate, the system and method can fully improve difference of the element classification system in artificial intervention degree in the coastal zone ecological environment study, and help to analyze coastal zone variation characteristics more objectively.

The invention discloses a saturated clay sample strength and strain softening parameter measuring device based on full-flow penetration, and belongs to the technical field of geotechnical, geology andenvironment research. The device mainly comprises two parts, namely, an overlying pressure loading system and a full-flow penetration system. Mainly for the problem of incomplete backflow of a soil body in a process of measuring soil body strength and strain softening parameters of a saturated clay by applying a full-flow penetrometer, by applying a working principle of a traditional consolidometer, the backflow of the soil body is guaranteed through vertical pressurization of a soil sample, so that the applicability of the full-flow penetrometer in the aspect of soil body strength and strainsoftening parameter measurement is improved, and an important practical value is achieved for experimental research and marine engineering design. The device can be applied to the measurement of laboratory soil samples and undisturbed soil samples in marine exploration, so that the undrained shear strength of the soil samples, the soil body strength after sufficient disturbance and the softeningparameters of the soil body are obtained. Finally reliable soil body material parameters are provided for design and stability evaluation of facilities such as seabed pipelines, ocean foundations andthe like.

The invention discloses a compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application of the compound microbial active filling material. Ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1 related in the invention are isolated bacteria obtained in a reactor for processing repugnant substances, such as H2S, in a water pollution control laboratory of the ecological environment research centre of the Chinese academy of sciences. The bacterial strains are preserved in the general microbial centre of the Chinese general microbial strain preservation and management committee. The preservation numbers are CGMCC No.7400 and CGMCC No.7399 respectively. The compound microbial active filling material disclosed by the invention has the advantages of being large in specific area, high in porosity, good in air permeability, low in resistance, high in bacterial cell loading capacity and less prone to run away; and because of being loaded with ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1, the compound microbial active filling material is difficult to decay and deform in a bioreactor for purifying waste gas, and therefore, the compound microbial active filling material can be used for a long time and is applied to complex processing conditions.

The invention discloses a root box experiment device for plant root environment research. The device comprises a front baffle, a rear baffle, a U-shaped root separating plate, a nylon net, a soil water collecting pipe, a water-holding base, a fastening screw, an air vent and a water inlet, wherein the nylon net is attached to the surface of the U-shaped root separating plate; the U-shaped root separating plate and the nylon net are located between the front baffle and the rear baffle and are fixed by the fastening screw; a soil water collecting hole is formed at a lateral edge of the U-shaped root separating plate; the water-holding base is located at the bottom. According to the invention, each separating plate and the water-holding base of the root box experiment device both are detachable and reusable, so that the technical problem of collecting the soil sample and soil water sample in the plant root environment research can be solved; a collecting method for millimeter-scale root tiny-region soil and soil water in different regions can be further simplified and accurate; the research on differential characteristics on different positions of the root system of the crop is benefited; the further accurate research on the root soil interface behavior is benefited.

The invention discloses a system and a method for separating and extracting soot generated by coal and biomass. The method comprises the following steps: heating a sampling gun unit, a cyclone cutter, a soot filter and a connecting pipe to 250 to 350 DEG C; putting the sampling gun unit into a flue of the coal and the biomass during heat utilization, and carrying out isokinetic sampling; preliminarily collecting the soot in flue gas on a filter membrane of the soot filter, scraping down initial soot, and adding a collected soot sample into an alcohol solution, and carrying out ultrasonic treatment and standing; pouring upper-layer liquid in a test tube into a crucible, and carrying out vacuum drying to obtain purified soot. The method for separating and extracting the soot in the flue gas generated by heat utilization of solid fuels such as the coal and the biomass, disclosed by the invention, can be used for quantitatively measuring the concentration of the soot in the flue gas. Solid particles of the purified soot can be used for medical and climatic environmental studies, and are of great significance to promote PM2.5 and chemical components thereof to effect human health and environment.

The invention discloses ochrobactrum anthropi SL1 and application thereof to removal of repugnant substances containing sulfur. Ochrobactrum anthropi SL1 is an isolate obtained from a reactor for treating repugnant substances containing H2S and the like in a water pollution control laboratory in Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. The strain is collected in China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.7400. The strain can be used for preparing microbial active fillers, is used for purifying the repugnant substances containing sulfur and solving the problem of odor pollution produced in the processes of sewage, sludge and refuse treatment and composting, and has more than 90% of efficiency of degrading repugnant substances containing sulfur such as hydrogen sulfide, methanethiol, dimethyl sulfide and the like.

The invention discloses a high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR) combined interface device, and belongs to the field of environmental studies. The device is composed of seven components: an optical table, a zinc selenide (ZnSe) crystal, a round drive rotary table, a heating casing pipe, an atomizer, a cleaning device and a drying device. A target pollutant enters a heating casing pipe and atomizer integrated device after being separated by a chromatography column, is heated and atomized, and then is evenly sprayed on the surface of the ZnSe crystal, and the ZnSe crystal is rotated to the optical table from the round drive rotary table. Infrared light enters the ZnSe crystal at a certain angle through the optical table, is reflected, then enters an FTIR detector and is subjected to atlas analysis. The ZnSe crystal having testing completed is transferred to the cleaning device and the drying device successively, is washed and dried respectively and then is re-used. The round rotary table carries the crystal and a sample for reciprocating detection by means of rotation, and sample batch processing and on-line detection are realized.

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and / or treatment of disease conditions.

The embodiment of the invention provides a river channel ecological monitoring system and method, and relates to the technical field of water ecological environment research. A detector module in a system is installed in a monitoring water area, sound wave signal sources are fixed on fish bodies in the monitoring water area, a signal receiver is electrically connected with the detector module, thesignal receiver is connected with a monitoring terminal, the detector module is configured to receive sound wave signals corresponding to the sound wave signal sources and send the sound wave signalsto the signal receiver, the signal receiver forwards the received sound wave signals to the monitoring terminal, and the monitoring terminal performs processing of the sound wave signals to obtain atwo-dimensional track and a three-dimensional track corresponding to each sound wave signal source. The river channel ecological monitoring system and method can more accurately determine a state of afish group through the obtained tracks and can more accurately obtain behavior response indexes of the fish group for environment change so as to achieve more accurate monitoring of an ecological condition of a river channel where the fish group live. The river channel ecological monitoring system and method have very important meaning of the water ecological environment research.

The invention discloses a method for marking germ cells and Sertoli cells of spermatogenic epithelium of turbot in different developmental stages and an application. Fragment Amh / Sox9 / Gsdf is amplified from cDNA of turbot spermary, cloning, preferring and plasmid extraction are performed sequentially, plasmids are linearized by restriction enzymes, probes are prepared after plasmid recovery, fixation, dehydration and entrapment are performed after sample processing, fluorescence in situ hybridization is performed after slicing, finally, nuclear staining is performed, and slice sealing, observation and photographing are performed. The method has the beneficial effects as follows: spermatids and the Sertoli cells of the spermatogenic epithelium of the turbot in different developmental stagesare separated out skillfully, and the marking method is simple and feasible and higher in precision; the method for marking the germ cells and the Sertoli cells of the spermatogenic epithelium of theturbot in different developmental stages can be applied to distinguishing and marking of cells of the spermatogenic epithelium of other marine organisms, and an effective method and a scientific basis are provided for studying spermatogenesis of fish and environment in the spermatogenic epithelium in future.

The invention belongs to the technology of deep rock mass construction characteristic research in the field of high-level waste geological disposal research and the filed of engineering geological research, and particularly relates to a drill core direction recovery method. The method comprises the following steps: 1, drawing core base lines; 2, recording core structural faces; 3, measuring and explaining a borehole TV to obtain a borehole wall distribution diagram; 4, calibrating a core, determining reference faces and determining inclination of the reference faces; 5, determining the direction of the base lines, wherein an inclination value Zi of the reference faces is acquired according to a borehole TV measuring result, and then the direction of the base lines can be obtained according to the following equation and the relative positions of the reference faces and the base lines; 6, determining the inclination of the core structural faces, wherein the inclined values of the structural faces within the ranges of the base lines are determined according to the following equation and the relative positions of the structural faces within the ranges of the base lines. According to the technical scheme, the drill core direction recovery method can achieve system recovery of deep drill core structural face occurrence and further provide technical support for deep geological environment research.

The invention discloses a reverse-flow type rapid extraction device for sediment organic matter and belongs to the field of environmental studies. The device comprises a sample treatment system (two round-bottom flasks containing a sediment sample and an organic extraction solvent respectively), a reverse-flow collecting system and a condensation reverse-flow system. The sediment sample, an ultrapure water mixture and the organic extraction solvent in the round-bottom flasks are heated respectively, the organic extraction solvent and target objects ascend to a condenser pipe at the upper end from a glass reverse-flow pipe in a gas state and a flow guiding mode, the organic extraction solvent extracts the target organic contaminants in a gas phase state, the target organic contaminants are condensed under the action of circulating cooling water and fall back into the reverse-flow collecting system, and due to the density difference between the organic extraction solvent and a water phase, an effective laying is obtained. The water phase and the organic phase return into the round-bottom flasks through return-flow catheters with different oblique angles, over and over again, the target organic contaminants are constantly extracted through the pure solvent, and the target organic contaminant is collected in the reverse-flow system. After extraction is completed, the organic phase containing the target objects is discharged through a liquid separating switch, components are collected, and therefore the target organic contaminants in the sediment sample are effectively extracted.

The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and / or treatment of disease conditions.

The invention discloses an Aquamicrobium defluvii active filler and a preparation method thereof. The Aquamicrobium defluvii SU1 disclosed by the invention is an isolate obtained by treating a reactor containing H2S and other odorants in Water Pollution Control Laboratory in Research Center for Eco-Environmental Sciences of Chinese Academy of Sciences; and the strain is collected in China General Microbiological Culture Collection Center, and the collection number is CGMCC No. 7399. The Aquamicrobium defluvii active filler without sulfur-containing odorants has the advantages of large specific area, high porosity, high air permeability, small resistance, high bacterial cell loading capacity and low loss tendency; the active filler with the Aquamicrobium defluvii SU1 has higher efficiency for degrading hydrogen sulfide; and the Aquamicrobium defluvii active filler also has the advantages of low filling density, low pressure and low tendency to corrosion and deformation in the waste gas purification bioreactor, and can be used for a long time and applicable to complex treatment conditions.

A capillary absorption spectrometer and process are described that provide highly sensitive and accurate stable absorption measurements of analytes in a sample gas that may include isotopologues of carbon and oxygen obtained from gas and biological samples. It further provides isotopic images of microbial communities that allow tracking of nutrients at the single cell level. It further targets naturally occurring variations in carbon and oxygen isotopes that avoids need for expensive isotopically labeled mixtures which allows study of samples taken from the field without modification. The method also permits sampling in vivo permitting real-time ambient studies of microbial communities.

The invention belongs to the technology of deep rock mass construction characteristic research in the field of high-level waste geological disposal research and the filed of engineering geological research, and particularly relates to a drill core direction recovery method. The method comprises the following steps: 1, drawing core base lines; 2, recording core structural faces; 3, measuring and explaining a borehole TV to obtain a borehole wall distribution diagram; 4, calibrating a core, determining reference faces and determining inclination of the reference faces; 5, determining the direction of the base lines, wherein an inclination value Zi of the reference faces is acquired according to a borehole TV measuring result, and then the direction of the base lines can be obtained according to the following equation and the relative positions of the reference faces and the base lines; 6, determining the inclination of the core structural faces, wherein the inclined values of the structural faces within the ranges of the base lines are determined according to the following equation and the relative positions of the structural faces within the ranges of the base lines. According to the technical scheme, the drill core direction recovery method can achieve system recovery of deep drill core structural face occurrence and further provide technical support for deep geological environment research.

The invention discloses a multi-stage intercepting and purifying device for suspended particulate organic matter, and relates to the field of water environment research. The multi-stage intercepting and purifying device is mainly used for intercepting and purifying various fine particulate suspended solids in a river channel. According to the technical points, the multi-stage intercepting and purifying device includes an aquatic plant filtering device, an electric flocculation device and an ultrafiltration membrane pool. The multi-stage intercepting and purifying device for the fine particulate suspended solids is provided in view of deficiencies in the prior art, and the multi-stage intercepting and purifying device can solve the multi-stage processing problem of the fine particulate suspended solids of the river channel and rivers, and is simple in structure, convenient to operate and low in cost.

The invention relates to a sediment collection device in a rice-fish interaction system and belongs to the field of fishery ecological environment research of aquatic science. The sediment collection device comprises a pipe A and a pipe B. The upper and lower ends of the pipe A are open and a first pull handle and a second pull handle are arranged on the upper end of the pipe A. The upper end of the pipe B is open and the lower end of the pipe B is closed. The upper end of the pipe B is provided with scales. The diameter of the pipe B is less than that of the pipe A. The sediment collection device realizes accurate acquisition of sediments of paddy rice having effective planting depth of 12-15 cm, can be operated simply and fast and realizes accurate acquisition of sediments at fixed depth.

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and / or treatment of disease conditions.

The invention discloses a root box experiment device for plant root environment research. The device comprises a front baffle, a rear baffle, a U-shaped root separating plate, a nylon net, a soil water collecting pipe, a water-holding base, a fastening screw, an air vent and a water inlet, wherein the nylon net is attached to the surface of the U-shaped root separating plate; the U-shaped root separating plate and the nylon net are located between the front baffle and the rear baffle and are fixed by the fastening screw; a soil water collecting hole is formed at a lateral edge of the U-shaped root separating plate; the water-holding base is located at the bottom. According to the invention, each separating plate and the water-holding base of the root box experiment device both are detachable and reusable, so that the technical problem of collecting the soil sample and soil water sample in the plant root environment research can be solved; a collecting method for millimeter-scale root tiny-region soil and soil water in different regions can be further simplified and accurate; the research on differential characteristics on different positions of the root system of the crop is benefited; the further accurate research on the root soil interface behavior is benefited.

The invention discloses a compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application of the compound microbial active filling material. Ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1 related in the invention are isolated bacteria obtained in a reactor for processing repugnant substances, such as H2S, in a water pollution control laboratory of the ecological environment research centre of the Chinese academy of sciences. The bacterial strains are preserved in the general microbial centre of the Chinese general microbial strain preservation and management committee. The preservation numbers are CGMCC No.7400 and CGMCC No.7399 respectively. The compound microbial active filling material disclosed by the invention has the advantages of being large in specific area, high in porosity, good in air permeability, low in resistance, high in bacterial cell loading capacity and less prone to run away; and because of being loaded with ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1, the compound microbial active filling material is difficult to decay and deform in a bioreactor for purifying waste gas, and therefore, the compound microbial active filling material can be used for a long time and is applied to complex processing conditions.

The invention relates to a digestion device for measuring total nitrogen in pond sediments and a using method of the digestion device for measuring the total nitrogen in the pond sediments, and belongs to the technical field of ecological environment research in aquaculture subject fishery. The digestion device comprises an electric furnace, digestion pipes, a first container, a second container, a condenser, an adsorption pipe and a vacuum pump, wherein multiple digestion pipes are arranged on the electric furnace and are communicated with one another by virtue of air inlet pipelines so as to stretch into below the liquid surface in the first container; the first container and the second container are connected by virtue of a first air outlet pipe; the first air outlet pipe stretches into the bottom of the second container; the second container and the condenser are connected by virtue of a second air outlet pipe; and the second container is sequentially connected with the adsorption pipe and the vacuum pump by virtue of pipelines. The whole measurement process is finished after pretreatment, sampling, digestion and tail gas treatment are finished. The digestion device disclosed by the invention comprises a digestion utensil and also contains a whole set of digestion tail gas treatment device, and the digestion process can be normally finished even if a ventilating hood is not used. The device provided by the invention is easy to operate, rapid, safe and free of pollution.

The invention relates to an environment DNA identification method for fish community structure researching. The method is characterized by comprising the steps that 1, water samples are collected according to the size of the water area where a fish community is located; 2, the water samples are processed, and total environment DNA of the processed water samples is extracted; 3, a 16s r DNA sequence with nucleotide sequences being SEQ ID NO:1 and SEQ ID NO:2 is adopted as a universal primer to carry out PCR amplification on the total environment DNA, and a PCR product is obtained; 4, gel electrophoresis is carried out on the PCR product, and gel with DNA is obtained; 5, gel cutting and clone sequencing are carried out on the gel with the DNA, and then blast comparison is carried out through GENBANK so as to determine whether the DNA is a fish sequence or not; 6, after it is determined that the DNA is the fish sequence, the next-generation sequencing technology is adopted for analyzing composition conditions of the PCR product on a large scale, and therefore the environment DNA fish composition and community structure are determined. The environment DNA identification method is quite easy, convenient and efficient and has the practical value.

The invention discloses bisphenol compound monosubstituted fatty alcohol and a synthesis method thereof. The bisphenol compound monosubstituted fatty alcohol has a general structural formula as shownin a formula I which is described in the specification. The preparation method has the advantages of simple operation, good atomic economy, good substrate applicability and wide applicability. The synthesized bisphenol compound monosubstituted fatty alcohol can be used as a novel halogenated flame retardant substitute, and is also applicable as a standard substance for derivatization thereof to analytical and environmental studies. The synthesis method provided by the invention can realize the preparation of halogen atom-substituted bisphenol substrates of different numbers, linear aliphatic hydrocarbon monosubstituted alcohols of different chain lengths, and linear aliphatic hydrocarbon monosubstituted terminal 1,2-diol compounds of different chain lengths, and has good universality.