85 results about "Exogenous hormones" patented technology

The invention relates to an artificial propagation method of yellow catfish fry, which is characterized in that: sexually mature yellow catfish over three years are chosen as the parent fishes; the female and male ratio is 3:1; a plurality of barrels are arranged in a spawning pond as fish nests; parent fish density in the spawning pond is 1 to 2 groups / m2; each group of parent fishes uses a barrel; the sand with a thickness ranging form 1cm to 5cm is arranged at the bottom of the barrel for the debonding of fish eggs; in spawning season, exogenous hormone is injected to the basal behind the dorsal fins of the parent fishes to induce ovulation and spermiation; an inversed conical container with smooth inner wall is used as the hatching barrel; fertilized eggs are arranged in the hatching barrel for hatching in flowing water; the dissolved oxygen in the water of the hatching barrel is larger than 5.5 mg / L; when hatching is over, fry is arranged in a cement pool for temporary rearing for 40 to 70 hours, then the fry is packaged in plastic bags with water and oxygen. The artificial propagation method of yellow catfish fry has the advantages that, well developed yellow catfish fry can be acquired; the operation is simple; the utilization rate of parent fishes is high; scale production of yellow catfish fry can be realized, and the invention has significant meaning for the intensive culture of yellow catfish.

The invention relates to a method for growing cell embryos of Dendrobium huoshanense, which comprises the following operating steps: (1) inducing sterile in-vitro cuttings; (2) inducing embryonic calli; (3) inducing somatic embryos; and (4) performing synchronous propagation of somatic embryos to obtain a large amount of cell embryos of Dendrobium huoshanense. In the method, morphological lower ends of the jointed stem segments of the sterile in-vitro cuttings are used as explants, the oxidation-reduction potential of the induction culture medium is controlled, exogenous hormone is not required to be added to induce the embryonic calli of Dendrobium huoshanensem, and the exogenous hormone is prevented from being enriched into complete plants through somatic cells and endangering health of human body. By controlling ambient humidity, embryonic calla are grown into somatic embryos, the consistency of spherical cell embryos is over 90 percent, and the survival rate of regenerated plants is over 85 percent. In the invention, the process is simple and not limited by time and seasons, and industrial automatic production of g cell embryos of Dendrobium huoshanense and in-vitro cuttings of Dendrobium huoshanense can be carried out in door for satisfying needs for production and planting.

A nutrient solution for rejuvenating trees in gardens is a nutrient solution, containing macroelements, trace elements, organic substances, physiologically active substances and exogenous hormones required for the growth of plants, prepared from ammonium nitrate, potassium nitrate, urea, magnesium sulfate heptahydrate, potassium dihydrogen phosphate, calcium nitrate, calcium chloride dihydrate, sucrose, ferrous sulfate heptahydrate, disodium ethylenediaminetetraacetate, zinc sulfate, copper sulfate, manganese sulfate tetrahydrate, sodium chloride, boric acid, vitamin A, vitamin C, vitamin B1,vitamin B6, humic acid, naphthaleneacetic acid, indolebutyric acid, 6-benzyladenine and gibberellin. The nutrient solution for rejuvenating trees in gardens can improve the survival rate of transplanted trees and promote and accelerate the self-recovering ability of nursery plants in order to make the nursery plants reach an expected landscape effect. The nutrient solution has a good rejuvenatingeffect on famous and old trees planted for many years and having poor growth vigor, and can enhance the resistance, break the dormancy, promote the germination of new shoots and new sprouts and delayaging.

The invention relates to a method for producing alkannin by utilizing Arnebia euchroma(Royle)Johnst hairy root. The method includes selection and processing of Arnebia euchroma(Royle)Johnst explant, activation culture of agrobacterium rhizogene, infection of cotyledon explant and induction of hairy root, obtaining of sterile Arnebia euchroma(Royle)Johnst hairy root system and liquid culture of Arnebia euchroma(Royle)Johnst hairy root as well as alkannin production. The used hairy root has the characteristic of rapid and autonomous growth on culture medium without exogenous hormones, thus the method not only can overcome the defects that cell culture grows slowly and hormone is required to be additionally added to maintain the growth thereof when utilizing cell culture to produce alkannin, and the method has the advantages of stable alkannin throughput, simple operation in cultivation, lower cost and no restriction of climate and land natural conditions. Especially the invention provides a stable and continuable novel medicine source for producing alkannin by utilizing hairy root culture method and provides reliable source and material basis for alkannin mass production by applying bioreactor in the future.

The invention discloses a method using exogenous hormone to regulate and control high fragrance camellia flowering phase and bloom quality; the method is characterized by using different exogenous hormone and concentration combinations to regulate and control different camellia flowering phases; the method uses different hormone concentration combinations to induce the camellia to bloom in advance or delay the bloom at different levels according to real needs, thus controlling the camellia bloom quality. The method is simple and convenient in operation, strong in practical value, and better in effect when compared with a conventional method of the same type; the method can more accurately and flexibly control the flowering phase and bloom quality, and can be directly applied to the camellia cultivation practice production works.

The invention discloses a method for the rooting culture of a sugarcane tissue culture seedling. The method comprises the following steps of: inoculating a multiplication sterile tissue culture seedling in a culture medium of MS (Murashige-Skoog), sodium hypochlorite with the concentration being 800-1200mg / L and 30g / L of white sugar, sealing, placing the mixture in a culture room with the temperature of 27+ / -1 DEG C, carrying out the rooting culture by illuminating for 12-16h every day, so as to realize the purpose that a complete root system grows after 20-30 days. According to the method disclosed by the invention, the culture medium does not contains exogenous hormones and does not need to be subjected to high-pressure sterilization, carries out the inoculation without an ultraclean working platform, and is simple and rapid to operate and low in pollution rate; the rooting culture cost can be reduced by 40-60 percent; the cultured rooting seedling grows strongly.

The invention discloses a method for artificially propagating pseudobagrus fulvidraco fries in autumn. The method comprises the following steps: (1) selecting and cultivating parents; (2) cultivating the parents; (3) laying eggs and fertilizing, namely a, carrying out artificial spawning by adopting exogenous hormones; and b, carrying out artificial insemination; and (4) carrying out incubation management. The method for artificially propagating the pseudobagrus fulvidraco fries is specific to summer and autumn high-temperature seasons, malformation rate of the fries is reduced, and effective rate and survival rate of the fries are improved.

The invention discloses a tissue culture formula for Euonymus phellomanus and a culture method. A sterile culture system is obtained by disinfecting explants, then tissue culture seedlings are subjected to subculture multiplication and growth and rooting are screened and regulated through matching of different kinds of exogenous hormones with different concentrations for screening and regulation, and the optimum explant and culture medium formula are determined finally and are enabled to adapt to industrial production. With adoption of the tissue culture formula and the method, the Euonymus phellomanus can be propagated massively, the landscaping requirement is met, the shapes of produced seedlings are stable, the production cycle is short, and large-scale rapid production can be realized. The tissue culture formula and the method are not limited by seasons, rapid multiplication is realized, the propagation coefficient is high, excellent features of a female parent can be inherited completely, and a problem is solved for factory production of tissue culture.

The invention discloses a method for improving the plumpness of direct-sowing rice grains through exogenous hormones and a growth regulator. The exogenous hormones ABA and Spm and monopotassium phosphate of the plant growth regulator are applied to the direct-sowing rice directly to improve the plumpness of the direct-sowing rice grains. The ABA or the Spm is sprayed in the early period of grouting of the direct-sowing rice, namely 9 days after flowering, and accordingly the corresponding endogenous hormone level can be significantly improved, the plumpness of the grains can be promoted, and the maturing rate, the plumpness of the grains and the thousand seed weight can be significantly improved, wherein the concentration of the ABA is 25 x 10-6 M and the concentration of the Spm is 5 x 10-4 M. The 500 g per mu of monopotassium phosphate is sprayed in the poured 3 leaf stage of the direct-sowing rice and accordingly the maturing rate, the plumpness and the thousand seed weight can be significantly improved and the yield can be improved by more than 10%. Accordingly, the plumpness of the rice grains can be significantly improved due to the fact that the suitable exogenous hormones and growth regulator are utilized in the suitable using period, the risk of the grouting of the direct-sowing rice grains can be avoided, the yield of the direct-sowing rice can be improved, the rice quality can be improved, and the production development of the direct-sowing rice can be promoted.

The invention discloses a method for improving the fruit yield of jatropha curcas through the transsexual regulation of male and female flowers, which comprises the following steps of: selecting jatropha curcas plants which survive after the planting, have the ability of flowering and bearing fruit, and have the bearing age of more than one year; performing local wound cleaning, disinfection and sterilization treatments on shaded sides of base parts of trunks and branches of the jatropha curcas; and applying a male and female flower regulator containing exogenous plant hormone preparation to the base parts of the treated trunks and branches, wherein the exogenous plant hormone preparation comprises the following components in parts by weight: 1-50 parts of S-abscisic acid, 1-20 parts of cyclodextrin, 1-20 parts of ethephon, 1-50 parts of cytokinin and 1-50 parts of D-isoascorbic acid. When the method is adopted, the formation of female flowers of the jatropha curcas can be improved through directed induction, and the total number of flowers and the ratio of female flowers to male flowers are obviously increased; therefore the yield of a single plant of the jatropha curcas can be improved by 3-5 times.

The invention provides a method for inducing somatic embryos of corn and belongs to the technical field of biological engineering. The method comprises the following steps of: inducing corn explants in a callus inducing culture medium; transferring the explants undergoing inducing culture to a subculture medium; propagating the somatic embryos in the subculture medium; and putting the propagated somatic embryos into differentiation culture medium for differentiation and seedling. By the method, a plurality of somatic embryos of corn can be induced with high efficiency, and the induced somaticembryos of corn have the characteristics of realizing the differentiation of both poles at the same time by long-time subculture infinite propagation and without the control of exogenous hormones, along with high differentiation rate and seedling rate; and because influence of exogenous hormones is avoided in the differentiation process, the mutation rate of somatic cells is relatively lowered atthe seedling stage of corn. The invention can provide an excellent and practical acceptor system for various trans-genetic methods (such as an agrobacterium-mediated method and a gene gun method).

The invention discloses a specific wavelength light control device capable of improving synchronous estrus efficiency of fur-bearing animals like rabbits, cats and martens and a control method thereof. The device comprises a specific wavelength light source and a light source controller, the light source controller comprises an intelligent dynamic time relay, a light intensity sensor and a PLC(programmable logic controller) processor, the specific wavelength light source is mounted above the middle of a shed for the fur-bearing animals like the rabbits, the cats and the martens, to-be-mated cages are arranged on two sides in the shed for the fur-bearing animals like the rabbits, the cats and the martens, and the light source controller is arranged on one side of a wall; the control device further comprises ear tag radio frequency recognizers, one ear tag radio frequency recognizer is mounted on one ear of each fur-bearing animal, and signals of the ear tag radio frequency recognizers are transmitted to the PLC processor. By the specific wavelength light control device and the control method, a specific wavelength light source can be emitted dynamically in a needed time period, light-sensitive characteristics of the fur-bearing animals like the rabbits, the cats and the martens can be utilized to induce synchronous estrus of the fur-bearing animals like the rabbits, the cats and the martens, tolerant effect on the fur-bearing animals like the rabbits, the cats and the martens by using exogenous hormones is eliminated, and labor input and production cost are lowered remarkably.

The invention discloses an in-vitro grafting method. According to the in-vitro grafting method, a scion and a stock are both in-vitro materials. After grafting, the stock which the scion is grafted onto is rapidly transplanted to a seedling bed, and the purpose that rooting of the stock and union of a connector are synchronous is achieved. By the adoption of the in-vitro grafting technology, grafting is easy to operate, centralization and industrialization of grafting are achieved, and multiple varieties with a low survival rate during traditional grafting or distant varieties can have a high survival rate after the environment optimization technology is adopted; especially, a good promoting function is achieved on inducing of callus tissue of a cut and formation of a fusion vascular bundle between heterogeneous cells, supplementation of a nutrient solution and exogenous hormones can be achieved in the integrally optimized seedling bed, and the union survival rate is greatly increased; the in-vitro grafting method has also the positive function on rapid cultivation of various induced seedlings during breeding.

The invention discloses a Polygala fallax Hemsl in-vitro rapid propagation and seedling breeding method. The method includes: taking Polygala fallax Hemsl tender stem as the explant, performing disinfection, then inoculating the explant to an initial medium of MS+6-BA 2.0mg / L+NAA 0.05mg / L to induce adventitious buds, then inoculating the adventitious buds to a proliferation and rooting medium attached with exogenous hormones of different types and concentrations to conduct proliferation and rooting culture, and transplanting the rooting test-tube plantlets into a seedling hardening matrix to perform seedling hardening. The Polygala fallax Hemsl in-vitro rapid propagation system established by the invention has the advantages of convenient material taking, good stability and high repeatability, and provides a method for realizing rapid propagation of Polygala fallax Hemsl high-quality germplasms and solving the current situation that the lack of wild resources causes demand exceeding supply in production.

The invention discloses a diagnostic kit and a diagnostic method. The composition comprises the following components: anti-mullerian hormone (AMH), assisted reproduction (ART), ovarian granuloma (GCT)and sinus follicle count (AFC), and the anti-mullerian hormone (AMH) is one of members of a transforming growth factor beta superfamily and is generated by recruitment of granulocytes of presinus follicles and small sinus follicles. The invention relates to the technical field of diagnostic kits. According to the diagnostic kit and the diagnostic method, AMH is not influenced by the menstrual cycle, the serum AMH level does not fluctuate obviously in the whole menstrual cycle, the kit can detect and measure the AMH level at any time and can relatively truly reflect the stock condition of original follicles and avoids the troubles and personal errors of sinus follicles (AFC) counting under B-ultrasonic waves and is more objective and accurate in detection result and is not affected by hormone contraceptives or exogenous hormones and is convenient for clinical use.

The invention relates to a method for inducing the wing shortening of Sogatella furcifera by utilizing an exogenous hormone, namely ZR-515 solution. The adopted technical scheme comprises the following steps of: freezing nymphs of the Sogatella furcifera at 3 to 4 ages, then dripping a small amount of juvenile hormone analogue, namely the ZR-515 solution on the backs of the nymphs, and raising the treated nymphs in a raising container for 2 to 8 days. The invention aims to design a novel, simple and convenient scheme for inducing the wing shortening of the Sogatella furcifera to improve the acquisition of short-winged individuals in the process of raising for experimental research materials, or provide a possible way for applying the method to fields and controlling the migration and dispersion hazard of the Sogatella furcifera in the future.

The invention provides an efficient subculture and rapid propagation method for ceratostigma willmottianum. The efficient subculture and rapid propagation method includes steps of shearing aseptic seedlings of ceratostigma willmottianum offspring to obtain explants, placing the explants in culture media without exogenous hormones and carrying out expanding propagation and proliferation culture onthe explants. The aseptic seedlings of the ceratostigma willmottianum offspring are obtained by means of preliminary tissue culture and breeding; each stem of each explant is provided with a leaf. Theculture media without the exogenous hormones are MS culture media with added sucrose and added agar. A preliminary tissue culture and breeding process includes acquiring tender stems of the upper half portions of ceratostigma willmottianum branches and utilizing the tender stems with buds as explants; sequentially carrying out auxiliary bud induction culture, auxiliary bud proliferation culture and rooting culture. The efficient subculture and rapid propagation method has the advantages that the breeding production cycle of the ceratostigma willmottianum can be shortened, and the production cost can be reduced; the efficient subculture and rapid propagation method has a large propagation coefficient and can be easily and feasibly implemented, the ceratostigma willmottianum can quickly grow, and materials can be acquired at any time, so that aseptic rapid propagation can be carried out.

The invention relates to a method for producing alkannin by utilizing Arnebia euchroma(Royle)Johnst hairy root. The method includes selection and processing of Arnebia euchroma(Royle)Johnst explant, activation culture of agrobacterium rhizogene, infection of cotyledon explant and induction of hairy root, obtaining of sterile Arnebia euchroma(Royle)Johnst hairy root system and liquid culture of Arnebia euchroma(Royle)Johnst hairy root as well as alkannin production. The used hairy root has the characteristic of rapid and autonomous growth on culture medium without exogenous hormones, thus the method not only can overcome the defects that cell culture grows slowly and hormone is required to be additionally added to maintain the growth thereof when utilizing cell culture to produce alkannin,and the method has the advantages of stable alkannin throughput, simple operation in cultivation, lower cost and no restriction of climate and land natural conditions. Especially the invention provides a stable and continuable novel medicine source for producing alkannin by utilizing hairy root culture method and provides reliable source and material basis for alkannin mass production by applyingbioreactor in the future.

The tissue culture formula and culture method of honeysuckle tartara of the present invention obtain a sterile culture system by sterilizing the explants, and then use the ratio of different types and different concentrations of exogenous hormones to subculture and grow the tissue culture seedlings Screening and regulation of rooting and rooting, and finally determine the best explant and medium formula, so that it can adapt to factory production. The tissue culture formula and method can reproduce honeysuckle tartar in large quantities to meet the needs of landscaping, and the produced seedlings have stable shape, short production cycle, and can meet large-scale rapid production. The invention can rapidly proliferate without being restricted by seasons, has a short breeding cycle, can completely inherit the excellent characteristics of the female parent, and solves a difficult problem for the industrialized production of tissue culture.

The invention provides an efficient subculture and rapid propagation method for ceratostigma willmottianum. The efficient subculture and rapid propagation method includes steps of shearing aseptic seedlings of ceratostigma willmottianum offspring to obtain explants, placing the explants in culture media without exogenous hormones and carrying out expanding propagation and proliferation culture onthe explants. The aseptic seedlings of the ceratostigma willmottianum offspring are obtained by means of preliminary tissue culture and breeding; each stem of each explant is provided with a leaf. Theculture media without the exogenous hormones are MS culture media with added sucrose and added agar. A preliminary tissue culture and breeding process includes acquiring tender stems of the upper half portions of ceratostigma willmottianum branches and utilizing the tender stems with buds as explants; sequentially carrying out auxiliary bud induction culture, auxiliary bud proliferation culture and rooting culture. The efficient subculture and rapid propagation method has the advantages that the breeding production cycle of the ceratostigma willmottianum can be shortened, and the production cost can be reduced; the efficient subculture and rapid propagation method has a large propagation coefficient and can be easily and feasibly implemented, the ceratostigma willmottianum can quickly grow, and materials can be acquired at any time, so that aseptic rapid propagation can be carried out.

The invention provides a control method for aphids of Zanthoxylum bungeanum, and belongs to the field of plant pest control. An exogenous hormone that is 2,6-dichloroisonicotinic acid and a methyl ester derivative (INA for short) thereof are adopted to spray Zanthoxylum bungeanum trees when the new-shoot aphid plant rate is larger than 4%, and the control rate reaches 90%. Traditional control methods using chemicals have problems that resistance is liable to generate in aphids , harm is caused to the environment and plants themselves, natural enemy insects of aphids can be killed, control durability is poor, and the like; compared with the traditional control methods, the disclosed control method adopts the exogenous hormone which is nontoxic, environment-friendly and free of harm to the environment and plants is used as an inducer to induce the Zanthoxylum bungeanum to generate resistance to the aphids, so that the growth and propagation of the aphids are inhibited, even the aphids are killed, the spreading of the Zanthoxylum bungeanum aphids is effectively controlled, the yield of the pricklyash peel is increased, and the income of farmers is increased.

The invention discloses a cutting propagation method of cryptomeria fortunei. The method includes the following steps that 1, cutting pretreatment is conducted; 2, exogenous hormones NAA50-150 ppm + IBA100-300 ppm are used, and cuttings are soaked for 0.5-1.5 h; 3, cutting is conducted; 4, after-management of cutting is performed. According to the cutting propagation method of cryptomeria fortunei, it is proved by experiments that under the conditions that exogenous hormones NAA150ppm + IBA100ppm are adopted and the cuttings are soaked for 1.5h, the rooting rate of the cryptomeria fortunei cuttings can reach 98.33%, the survival rate of the cuttings is increased, and the root growth can be promoted. The method is simple and feasible, and reliable seedling sources are provided for fast-growing and high-yield cryptomeria fortunei forest planting.

The invention relates to an asparagus composition capable of preventing and recuperating climacteric symptoms, and belongs to the technical field of foods. The asparagus composition comprises the following components compatible in parts by mass: 240-300 parts of an asparagus extract, 16-24 parts of casein bioactive peptide, 16-24 parts of a purple sweet potato extract, 30-40 parts of a hippophae rhamnoides fruit extract, 18-30 parts of a rhizoma polygonati extract, 10-20 parts of a folium perillae extract, 6-10 parts of gamma-aminobutyric acid and 4-8 parts of theanine. The asparagus composition can also be applied to preparation of foods, health-care foods or medicines for preventing and recuperating the climacteric symptoms. The composition disclosed by the invention can enhance the antioxidant ability of human bodies, regulate the stability of an autonomic nervous system, improve the secretion level of neurotransmitters, reduce blood pressure and reduce blood lipid, and under the situation that no exogenous hormones or estrogen components are added, the asparagus composition can fundamentally recuperate the functions of the whole tissue, delay the occurrence and the developmentof climacterium and improve the symptoms of climacterium syndromes.

The invention relates to an artificial propagation method of yellow catfish fry, which is characterized in that: sexually mature yellow catfish over three years are chosen as the parent fishes; the female and male ratio is 3:1; a plurality of barrels are arranged in a spawning pond as fish nests; parent fish density in the spawning pond is 1 to 2 groups / m2; each group of parent fishes uses a barrel; the sand with a thickness ranging form 1cm to 5cm is arranged at the bottom of the barrel for the debonding of fish eggs; in spawning season, exogenous hormone is injected to the basal behind the dorsal fins of the parent fishes to induce ovulation and spermiation; an inversed conical container with smooth inner wall is used as the hatching barrel; fertilized eggs are arranged in the hatching barrel for hatching in flowing water; the dissolved oxygen in the water of the hatching barrel is larger than 5.5 mg / L; when hatching is over, fry is arranged in a cement pool for temporary rearing for 40 to 70 hours, then the fry is packaged in plastic bags with water and oxygen. The artificial propagation method of yellow catfish fry has the advantages that, well developed yellow catfish fry canbe acquired; the operation is simple; the utilization rate of parent fishes is high; scale production of yellow catfish fry can be realized, and the invention has significant meaning for the intensive culture of yellow catfish.

The invention relates to the technical field of cutting planting of litsea coreana, and discloses a litsea coreana tree cuttage planting method with a high survival rate. The method comprises the following steps of S1, selecting a site; S2, preparing land; S3, constructing a protective belt, namely constructing a protective forest belt around the land according to the specific situation of the divided planting land so as to protect the growth of tea trees; S4, cultivating land; S5, correcting cutting seedlings; S6, transplanting cutting wood; S7, performing shading protection; S8, carrying outillumination training; and S9, performing pruning and shaping. The litsea coreana tree cuttage planting method with the high survival rate has the advantages that through the treatment effect of an exogenous hormone naphthylacetic acid solution and high-concentration rooting powder, rooting of cutting slips can be effectively promoted, and the cuttage survival rate is effectively increased.

The invention discloses a method for simultaneous detection of seven endogenous hormones in wheat leaves. The endogenous hormones are ZR, ZT, JA, SA, ABA, GA3 and GA4. UPLC-MS / MS is employed in the invention, and chromatographic conditions are that 0.1% acetonitrile is used as a mobile phase A; an aqueous solution of 0.05% formic acid is used as a mobile phase B; and methanol is employed for gradient elution. The method of the invention has the advantages of high specificity, high sensitivity, high throughput and objective and easy-to-analyze results, can determine the imbalance of endogenous hormones during the growth and development of wheat and provide guidance for supplementation of exogenous hormones of wheat, and reduces occurrence of blind supplementation of exogenous hormones.

The invention discloses a method for promoting the flowering of saplings of camellia nitidissima. The method includes the operation steps that (1) breeding of the saplings is carried out, specifically, mature fruits of camellia nitidissima are collected, dried and sown, and nursery stock planting can be carried out after a certain period of sapling management; (2) nursery stock planting is carriedout; (3) nursery stock management is carried out; (4) girdling is carried out, specifically, 6-year-old camellia nitidissima saplings are selected, a girdling knife is used to carry out girdling on trunks 5-10 cm from the ground, and the girdling width is 2.5-4.0mm; (5) exogenous hormone treatment is carried out, specifically, exogenous hormone is used to spray the tree bodies girdled in the step(4). The saplings of camellia nitidissima treated by the method are all bloomed, and the average number of flowers per plant is 42, compared with plants without any treatment, the rate of floweringplants is increased by 60%, and the number of flowers is increased by 10.5 times. The flowering rate and the flowering number of the saplings of camellia nitidissima are significantly improved, the early flowering and early production of camellia nitidissima are realized, and the method is of great significance for promoting the development of the camellia nitidissima industry.

The invention relates to a production method of organic sprouts of small black bean and is intended to overcome the defect that a traditional method has low yield and depends on exogenous hormones andpesticides. The production method includes: selecting annual seeds of small black bean having disease resistance and complete kernels and having hundred-grain weight of 6.1-12 g; washing the seeds with clear water, and soaking the seeds in a culture liquid, made by mixing sprout probiotics and water in the mass ratio of 1:1000, for 6-12 hours; accelerating germination in a germination accelerator; broadcasting the seeds into seedling trays under seeding density of 0.25-0.32 g / cm<2>; stacking the seedling trays, with one empty tray at the top and one empty tray at the bottom, placing the trayson a ground in a dark shop, allowing growth at 20-25 DEG C for 12-18 hours, and spreading on a cultivation frame; spraying a nutrient liquid every day with a sprayer, with temperature controlled to 15 DEG C< / T< / =32 DEG C; ventilating once at 8-9 PM and once at 8-9 PM each day; harvesting when hypocotyl length reaches 10-15 cm.

The invention discloses application of bacillus licheniformis to the promotion of rooting or growth of semen trigonellae tissue culture seedlings. The application comprises the bacterium activation of a raw material entrocoor dinatibiogen and inoculation to successive semen trigonellae seedlings in form of bacillus licheniformis liquid, a large number of separation, purification and strain identification steps are eliminated, the addition of any exogenous hormones is avoided, the cost is low, the operation is easy, safe and nontoxic, and remarkable effects are achieved; the rooting rate of the inoculated semen trigonellae tissue culture seedlings is remarkably increased, indexes such as plant height and leaf area are also improved, the survival rate after fungal contamination is greatly increased, and the rooting quality of the semen trigonellae tissue culture seedlings is remarkably improved.

The invention discloses a cryptomeria fortunei cutting propagation method which includes step 1) of performing cutting pretreatment; step 2) of adopting exogenous hormones NAA 50-150 ppm + IBA 100-300ppm, and soaking cuttings 0.5-1.5 h; step 3) of performing cutting, and step 4) of performing management after cuttings. According to the cryptomeria fortunei cutting propagation method, tests provethat under the conditions of adopting the exogenous hormones NAA 150 ppm + IBA 100 ppm to soak the cuttings for 1.5 h, the cryptomeria fortunei cutting rooting rate can reach 98.33%, root growth can be promoted while the cutting survival rate is improved, the method is simple and practical, and reliable nursery stock sources are provided for the construction of fast-growing and high-yield forestsof cryptomeria fortunei.