70 results about "Fatty acid synthesis" patented technology

The present invention is directed to nucleic acid molecules and nucleic acid constructs, and other agents associated with fatty acid synthesis, particularly the ratios of saturated and unsaturated fats. Moreover, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of saturated and unsaturated fats. In particular, the present invention is directed to plants incorporating such agents where the plants exhibit altered levels of saturated and unsaturated fatty acids.

Cytochrome b5 (Cb5) is a haem-binding protein located in the endoplasmic reticulum (ER) and the outer mitochondrial membranes of higher eukaryotes. In higher plants, animals, and fungi, the ER resident Cb5 has been shown to play a role in desaturation of acyl CoA fatty acids. Higher plants Cb5 isoforms from plants such as soybean or Arabidopsis are capable of modulating omega-3 desaturation. Co-expression of certain Cb5 isoforms with FAD3 in a host plant results in increased production of seed oil content as well as altered ratio between different fatty acids. It is also disclosed here that overexpression of Yarrowia ACL enzymes in the plastids of a host plant helps boost the synthesis of acetyl CoA, which in turn, may lead to increased synthesis of fatty acids and enhanced oil accumulation in the seeds.

The present invention relates to a method of treating a fatty acid synthesis related disease comprising administering to a patient in need thereof an effective amount of a PARP inhibitor or metabolite thereof to inhibit fatty acid synthesis, wherein the fatty acid synthesis related disease is obesity, diabetes, or cardiovascular disease. The present invention also relates to a method of treating a cancer in a subject comprising: (i) identifying a level of fatty acid in a sample from the subject, and (ii) administering an effective amount of a PARP inhibitor or metabolite thereof to inhibit fatty acid synthesis in the subject, wherein the administration is based on the level of fatty acid, thereby treating the cancer in the subject. The present invention further relates to a method of treating Her-2 related cancers by administering to a patient in need thereof an effective amount of a PARP inhibitor or metabolite thereof to inhibit fatty acid synthesis.

The present invention is directed to nucleic acid molecules and nucleic acid constructs, and other agents associated with fatty acid synthesis, particularly the ratios of saturated and unsaturated fats. Moreover, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of saturated and unsaturated fats. In particular, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of monounsaturated to polyunsaturated fatty acids.

The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.

The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.

The invention provides an application of lactobacillus plantarum powder in regulating fat metabolism and intestinal flora of fat rats. The lactobacillus plantarum powder can reduce the in-vivo fat content, increased under induction of high-fat diet, of rats and reduce the body fat rate; the FFA content, increased under induction of high-fat diet, in serum of rats can be remarkably reduced; meanwhile, the TG content in the serum of obese rats can be reduced, and the HDL-C content is increased; the lipid metabolism function of the livers of the obese rats can be improved, and activation of a fatty acid synthesis pathway of the obese rats is inhibited; the condition of intestinal flora disorder of the obese rats can be improved, and the flora richness, flora diversity, flora structure and composition of the obese rats can be maintained; increase of relative abundance of firmicutes is inhibited; the relative abundance of bacteroides is improved; the relative abundance ratio of the firmicutes to the bacteroides in intestinal tracts of rats under the condition of high fat diet is reduced; and the similarity of intestinal flora of the obese rats and healthy rats is increased.

Methods of producing fatty acid esters, such as fatty acid ethyl esters, from microorganisms genetically engineered to alter the expression of genes involved in fatty acid synthesis and / or ethanol production, such that a microorganism is obtained that can produce an alcohol, a fatty acid and an ester synthase in the absence of exogenously added alcohols.

This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl-acyl carrier protein reductase (acyl-ACP reductase). These microorganisms may optionally overexpress one or more fatty acid synthesis proteins such as ACP and ACCase, and / or one or more polypeptides associated with glycogen breakdown. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids or wax esters and / or synthesizing triglycerides.

Disclosed are improved cleaning compositions comprising soap&com ma; fatty acid, synthetic detersive surfactant, salt and a polyhydridic alcohol, which are suitable for formation into precursor cleansing / laundry bar “soap noodles,” personal cleansing bars and laundry detergent bars. The compositions comprise: (a) from about 58% to about 93% by weight of an approximately 70% aqueous soap slurry; (b) from about 1% to about 15% by weight of a fatty acid; (c) from about 2% to about 30% by weight of an approximately 55% aqueous mixture of anionic surfactants comprising i) an alpha sulfonated alkyl ester, and ii) a sulfonated fatty acid or salts thereof, wherein the ratio of i) to ii) is from about 10:1 to about 1:10; (d) from about 0.5% to about 2% by weight of a salt; (e) from about 0.5% to about 5% by weight of a 25 polyhydridic alcohol; and (f) from 0 to about 10% by weight of an alkanolamide of the formula O CH3(CH2)nCNH(CH2)yOH wherein n=6-16, and y is 2-4; The invention additionally relates to an improved process for producing both precursor cleansing / laundry bar “soap noodles” and personal cleansing / laundry detergent bars 35 comprising combining (a)-(e) to form a liquid mixture at a temperature of about 65° C. to about 105° C., removing from about 50% to about 90% by weight of the water from the liquid mixture, by heating up to 150° C. under vacuum conditions or 105° C. at normal conditions to form a thickened mixture, extruding the thickened mixture to form flaked solid or semi-solid pellets or noodles, and optionally, plodding the flaked solid or semi-solid pellets or noodles to form plodded pellets or noodles,f extruding the plodded pellets or noodles to form a billet, cutting the billet, and stamping the cut billet to yield a personal cleansing / laundry detergent bar.

The presently disclosed subject matter relates to metabolic reprogramming agents that decrease glutamine metabolism, glycolysis, and fatty acid synthesis, pharmaceutical compositions comprising at least one, at least two, or at least three metabolic reprogramming agents, and the use of those agents and compositions for treating metabolic reprogramming disorders, such as immune disorders (e.g., autoimmune diseases), inflammatory diseases, and transplant rejection, pathologies due to CNS inflammation due to infection and not involving infection, and neurodegenerative disorders.

The invention relates to the technical field of synthetic biology, in particular to a genetic element, an expression vector and application of the genetic element and the expression vector. The invention provides a novel method for improving the synthesis of fatty hydrocarbon microorganisms: the fatty hydrocarbon synthesis capability of escherichia coli is improved by dynamically balancing accumulation and conversion of a key intermediate metabolite, namely fatty aldehyde. In order to achieve this purpose, ADO is helped to balance the synthesis and conversion of the fatty aldehyde through a competition path by synergistically expressing a synthesis process of fatty alcohol; then, three modules for fatty acid synthesis, lipid degradation and electron transfer, which are related to dynamic balance of the fatty aldehyde, are matched with a fatty hydrocarbon synthesis module, and finally the artificial synthesis of high-yield fatty hydrocarbon from engineering escherichia coli is realized.

The invention relates to compounds with selective inhibitory activity against aldolase and a preparation method thereof, pharmaceutical compositions containing the compounds, and application of the compounds to preparation of drugs for inhibiting the synthesis of triglyceride and cholesterol, drugs for reducing the synthesis of fatty acid, drugs for preventing and / or treating obesity and type 2 diabetes, drugs for preventing and / or treating tumors, drugs for preventing and / or treating Parkinson's disease, drugs for preventing and / or treating Alzheimer's disease, or drugs for prolonging the life of mammals. The general structure of the compounds is as described in the specification.

The present invention provides for a genetically modified yeast host cell capable of producing one or more fatty acids, or fatty acid-derived compounds, or a mixture thereof, comprising: (a) increased expression of acetyl-CoA carboxylase (such as ACC1), (b) increased expression of one or more fatty acid synthases (such as FAS1 and FAS2), and (c) optionally reduced expression of one or more enzymes involved in or in the β-oxidation pathway (such as peroxisomal transporters PXA1 and PXA2, and β-oxidation enzymes POX1, POX2, and POX3).

The presently disclosed subject matter relates to metabolic reprogramming agents that decrease glutamine metabolism, glycolysis, and fatty acid synthesis, pharmaceutical compositions comprising at least one, at least two, or at least three metabolic reprogramming agents, and the use of those agents and compositions for treating metabolic reprogramming disorders, such as immune disorders (e.g., autoimmune diseases), inflammatory diseases, and transplant rejection, pathologies due to CNS inflammation due to infection and not involving infection, and neurodegenerative disorders.

Recombinant nucleic acids and vector constructs encoding acyltransferases and variant thioesterases, and the acyltransferases and variant thioesterases encoded by the nucleic acids are provided. The acyltransferases and variant thioesterases are useful in fatty acid synthesis and triacylglycerol production. Host cells that express the recombinant nucleic acids as well as methods of cultivating thehost cells, methods of producing oils from the host cells are provided. The recombinant host cells and the oils produced therefrom have altered fatty acid profiles and / or triacylglycerols with altered regiospecificity.

The invention discloses weight-reducing health protection tea which is prepared from the following materials in parts by weight: 20-40 parts of semen cassiae, 20-40 parts of lotus leaf, 10-30 parts of ginseng and 10-30 parts of camellia chrysantha. The tea is rich in various effective weight-reducing health protection substances including tea polyphenol, total flavonoids, nuciferine, ginsenoside, vitamin, amino acid and trace elements, can convert redundant fat of the human body through manners of promoting lipolysis, inhibiting fatty acid synthesis, regulating substance metabolism and relaxing bowels, and can recover the energy metabolism of the human body, thus achieving the expected purpose of healthy weight reducing. Furthermore, the tea also has good health protection effect, can regulate the central nervous system of the human, strengthen the heart and resist fatigue, can achieve good qi-blood circulation of the body, and has good treatment effect on multiple diseases of the nervous system, the cardiovascular system, the endocrine system and the reproductive system.

The invention relates to a DNA sequence for coding Myrmecia incisa delta-6 fatty acid elongase, which comprises: a, the nucleotide sequence in SEQIDNO.10; or b, the nucleotide sequence complementary to the nucleotide sequence in a. The invention also provides a recombinant expression vector and genetically engineered host cells of the nucleotide sequence and the application thereof. The advantages of the invention are as follows: it is testified through the molecular cloning and functional identification of the Myrmecia incisa delta-6 fatty acid elongase gene that the gene code is delta-6 fatty acid elongase; the application provided by the invention provides a theoretical basis for improving the capability of long-chain polyunsaturated fatty acid synthesis from oil crops, and theoretically, the gene provided by the invention can be introduced into oil crops to effectively prolong the precursors that are necessary for the synthesis of arachidonic acid such as gamma-linolenic acid and endow the oil crops with the capability of the long-chain polyunsaturated fatty acid synthesis so as to improve the quality of the oil crops.

The invention relates to a strategy for growth and metabolism regulation of microalgae cultured by urban sewage. The strategy mainly includes: (1) in an early culture stage, a plant growth regulator and COD (chemical oxygen demand) degrading bacteria are added into microalgae-containing urban sewage to promote chlorophyll synthesis and degradation of organics in sewage and improve microalgae cellphotosynthetic efficiency and carbon sequestration efficiency to increase biomass yield; (2) in a later culture stage, the nitrogen content of sewage meets a nitrogen deficiency condition, and accordingly a glycolytic pathway is regulated so as to promote carbohydrate metabolism to enable more carbon for fatty acid synthesis, and grease accumulation promotion is realized. Adopting the urban sewagefor culturing microalgae meets the requirement of a two-stage culture process in a same system, both microalgae high-density culture and grease accumulation can be achieved, simplicity and convenience in operation are achieved, and large-scale production is facilitated; the strategy is significant to guidance of large-scale microalgae culture and provides theoretical and technical support for process optimization of an industrial chain for preparing biodiesel from microalgae cultured by urban sewage.

The present invention provides for a genetically modified yeast host cell capable of producing one or more fatty acids, or fatty acid-derived compounds, or a mixture thereof, comprising: (a) increased expression of acetyl-CoA carboxylase (such as ACC1), (b) increased expression of one or more fatty acid synthases (such as FAS1 and FAS2), and (c) optionally reduced expression of one or more enzymes involved in or in the β-oxidation pathway (such as peroxisomal transporters PXA1 and PXA2, and β-oxidation enzymes POX1, POX2, and POX3).

The invention relates to the technical field of refrigerating machine oil products, in particular to base oil of refrigerator oil, refrigerator oil containing the base oil and a working fluid composition for a refrigerating machine. The base oil of refrigerator oil is ester synthesized from pentaerythritol and a plurality of fatty acids, the fatty acids comprise a first fatty acid, the first fattyacid is a C5 straight-chain and / or branched-chain monobasic fatty acid; the fatty acids further comprise at least one of a second fatty acid and a third fatty acid, wherein the second fatty acid is aC8-C9 branched-chain monobasic fatty acid, and the third fatty acid is binary fatty acid of C5-C8. The refrigerating machine oil and the working fluid composition for the refrigerating machine provided by the invention can simultaneously consider the intermiscibility, lubricity, thermal stability and / or chemical stability of the refrigerant when the R32 refrigerant is used.

The invention discloses a method for synthesizing unsaturated polyester by using C36 dimer fatty acid. The method comprises the following steps: (1) a performed polymer of C36 dimer fatty acid, phthalic anhydride, and 1,2-propylene glycol is prepared by using a direct melt polycondensation method; (2) unsaturated polyester is prepared by a reaction of a product in the step (1) and maleic anhydride.

Compounds that are fatty acid synthesis modulators are provided. The compounds may be used to treat disorders characterized by disregulation of the fatty acid synthase function by modulating the function and / or the fatty acid synthase pathway. Methods are provided for treating such disorders including viral infections, such as hepatitis C infection, cancer and metabolic disorders, such as non-alcoholic steatohepatitis (NASH).

The invention provides a culture method for increasing the grease content of saccharomyces cerevisiae by maintaining an ultralow-concentration organic carbon source and application of the culture method and belongs to the technical field of biology. Research finds that in the fermentation culture process of saccharomyces cerevisiae, the concentration of an organic carbon source in a fermentation culture solution is continuously maintained at an ultra-low level by controlling the supplementing amount of the organic carbon source, and in the state, activity of enzymes related to ethanol synthesis in saccharomyces cerevisiae cells is greatly reduced due to lack of an acting substrate, so activity of the enzymes related to ethanol synthesis in saccharomyces cerevisiae cells is improved. However, activity of enzymes related to fatty acid synthesis is not obviously influenced, so the flow direction of an organic carbon source deviates to fatty acid synthesis, and finally, a large amount of grease in saccharomyces cerevisiae cells is accumulated. The method is advantaged in that raw materials are low in cost, the culture method is simple and easy to implement, industrial production and application of the method are facilitated, and therefore the method has good actual production and application value.

The invention discloses recombinant rice capable of reducing cholesterol and a preparation method thereof. The recombinant rice comprises sorghum, oat, black rice, buckwheat, barley and rice. Through being metered by 100 percent of the total mass of raw materials, the content of the sorghum flour is 0 to 30 percent, the content of the oat is 0 to 60 percent, the content of the black rice is 0 to 60 percent, the content of the buckwheat is 0 to 70 percent, the content of the barley is 0 to 30 percent, and the content of the rice is 0 to 30 percent. According to the recombinant rice formula capable of reducing cholesterol, the specific formula of the recombinant rice is optimized, so that the TC and LDL-C of the liver are remarkably reduced, the HDL-C content is remarkably increased, slight liver injury is repaired, and the health of the liver is facilitated; and meanwhile, a certain promoting effect is achieved on synthesis of bile acid, the RCT of cholesterol is enhanced, the accumulation effect of LDL-C in blood is reduced, and a new effect is achieved on promoting synthesis of fatty acid.

The invention relates to methods for producing lipids such as fatty acid products in recombinant host cells engineered to express a non-native gene encoding a dehydrogenase. The recombinant microorganisms are able to proliferate at a higher rate as compared with microorganisms that do not express a non-native dehydrogenase gene, and cultures of microorganisms that are engineered for lipid production and that express a non-native dehydrogenase produce more lipid than cultures of control microorganisms that do not include a non-native dehydrogenase gene.

The invention relates to application of ssl2084 gene in synthesis of medium and long chain fatty acids. The ssl2084 is a protein capable of promoting synthesis of the medium and long chain fatty acidsin synechocystis, and has an amino acid sequence shown as SEQ ID NO. 2. The invention further relates to application of the ssl2084 gene in synthesis of medium and long chain saturated fatty acids ofthe synechocystis. For the first time, the invention discloses an important role of the ssl2084 gene of the synechocystis sp. PCC 6803 in synthesis of the medium and long chain saturated fatty acidsat low temperature. By increasing expression of the ssl2084 gene in the synechocystis sp. PCC 6803 by utilizing an overexpression method, contents of C12:0, C16:0 and C18:0 in mutant strains are significantly increased; and moreover, the contents of the C12:0, the C16:0 and the C18:0 in the mutant strains are further increased after low-temperature treatment, so that the recognition that it is easy to synthesize unsaturated fatty acids at low temperature in the field is changed.

The invention relates to a hair-restoring hair-caring hair-nourishing liquid; the hair-nourishing liquid promoting hair to grow comprises the components: an alcohol leaching solution (a solution A) composed of radix polygoni multiflori, raw ginger, cypress branches, blister beetle and anhydrous alcohol; an alcohol solution (a solution B) composed of pepper oil, table salt, glycerol, alkyl phenol polyoxyethylene, retinoic acid, erucic acid, peppermint oil, Tween 80 and anhydrous alcohol; and an aqueous solution (a solution C) composed of allantoin, vitamin B6, vitamin C and purified water. The solution A, the solution B and the solution C are mixed uniformly to synthesize the hair-restoring hair-caring hair-nourishing liquid. The hair-restoring hair-caring hair-nourishing liquid has the effects of adjusting fatty acid synthesis, suppressing sebum secretion, reducing too much scalp grease, maintaining normal metabolism, and stimulating and promoting hair growth, and has obvious curative effect on scalp folliculitis, alopecia, alopecia areata, bald head and other symptoms.