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56 results about "Helicoverpa armigera" patented technology
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The cotton bollworm, corn earworm, or Old World (African) bollworm (Helicoverpa armigera) (also known as the scarce bordered straw in the UK, where it is a migrant) is a moth, the larvae of which feed on a wide range of plants, including many important cultivated crops. It is a major pest in cotton and one of the most polyphagous and cosmopolitan pest species. It should not be confused with the similarly named, related species Helicoverpa zea.
Bacillus thuringiensis MB-15 strain and preparation method of its wettable powder
InactiveCN103160449ABroad insecticidal spectrumGood control effectBiocideBacteriaCotton bollwormInsecticidal crystal proteins
The invention discloses a Bacillus thuringiensis MB-15 strain which has a broad spectrum and high virulence on vegetable lepidoptera pests, and the preservation number of the strain is CGMCC No. 5255. The strain can form a diamond insecticidal crystal protein, contains cry1I, cry1Ac, cry2Ac and vip3Aa genes, and has high-efficiency insecticidal activity on plutella xylostella, Pieris brassicae, beet armyworm, Spodoptera litura, Helicoverpa armigera and other lepidoptera pests. The invention also relates to a preparation method of the wettable powder of strain. The wettable powder comprises the following content of main components: 40.0-50.0% of Bacillus thuringiensis MB-15 strain powder, 5.0-10.0% of a dispersant, 5.0-10.0% of a wetting agent, 0.5-1.0% of a stabilizer, 0.5-1.0% of an ultraviolet light protection agent, and 28.0-49.0% of a carrier. The wettable powder can be used to control a plurality of lepidoptera pests on vegetables, and has the characteristics of broad spectrum, high efficiency, and safety, etc.
Owner:HEBEI AGRICULTURAL UNIV.
A plurality of indolocarbazole alkaloids having strong insect disinfestation activities, and production strain thereof
InactiveCN104593281AStable characteristicsNo pollution in the processBiocideSugar derivativesArtogeia rapaeMycangium
The present invention discloses a plurality of indolocarbazole alkaloids having strong insect disinfestation activities, and a production strain of the indolocarbazole alkaloids. The indolocarbazole alkaloid compound production strain CQT14-24 is a marine Streptomyces nitrosporeus CQT14-24 strain, wherein the strain is subjected to artificial culture, and the obtained fermentation product is extracted by the conventional chemical method so as to obtain four indolocarbazole alkaloids and an anthraquinone compound. According to the present invention, the indolocarbazole alkaloids and the one anthraquinone compound provide strong killing effects on a variety of cultivated plant pests, particularly the pests having characteristics of spreading in agriculture and forestry production and serious harm and comprising Pieris rapae, Brevicoryne brassicae, Hyphantria cunea, Plutella xyllostella, Mythimna separate and Helicoverpa armigera, wherein the relative mortality rate achieves 81.9-100%, the polypide is seriously reduced, the growth inhibition rate is 100%, and the imago pupation rate is reduced by 70.4-86.7%; and the strain is subjected to acclimation through the artificial culture, and has characteristics of stable feature, easy culture, simple and feasible operation method, no environmental pollution, short period, cheap raw materials, low production cost, good development prospect, and easy industrialization achieving.
Owner:王林
Biological assaying method for evaluating effect of transgene cotton on chrysopa pallens
ActiveCN104133054AWide applicabilitySolve the preservation problemBiological testingChrysopa pallensHigh doses
The invention provides a biological assaying method for evaluating the effect of transgene cotton on chrysopa pallens. The method comprises the following steps: paving agar on the bottom of a culture dish; placing transgene cotton leaves on the surface of the agar, inoculating newly-hatched helicoverpa armigera larvae; feeding newly-hatched chrysopa pallens larvae for 1 to 3 days with pea aphid, then feeding the chrysopa pallens larvae with the helicoverpa armigera larvae which has been fed by the transgene cotton leaves for 1 to 3 days; observing the growth situation of the chrysopa pallens larvae since the first day when the chrysopa pallens larvae is fed with the helicoverpa armigera larvae until the chrysopa pallens larvae pupate and perform eclosion; setting a reference group; comparing the differences of the experiment group and the reference group on the indexes of pupation rate, eclosion rate, larva weight, imago weight, and total growth time, and analyzing the difference significance through variance analysis so as to evaluate the effect of transgene cotton on chrysopa pallens. The application range of the biological assaying method is wide. The effect of high dose protein in transgene plants on predators can be effectively measured by utilizing the nutrition relationship evaluation of transgene plant-pest-predator.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Helicoverpa armigera V-ATPase A gene cDNA and application thereof
InactiveCN104342448AWide range of hostsReduced risk of developing resistanceBiocideHydrolasesBiotechnologyGossypium
The invention discloses helicoverpa armigera V-ATPase A gene cDNA and application thereof. Helicoverpa armigera V-ATPase A siRNA is designed and synthesized, after helicoverpa armigera takes V-ATPase A siRNA in, expression of V-ATPase A gene is blocked, growth and metabolism of helicoverpa armigera are inhibited, the mortality is raised, and therefore helicoverpa armigera V-ATPase A gene cDNA is applicable to biological control of helicoverpa armigera.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Cotton bollworm calcium mucoprotein gene fragment hacad1 with insect disinfestation building action and uses thereof
InactiveCN101492681AHigh insecticidal activityOvercoming or delaying the problem of drug resistanceBiocideBacteriaEscherichia coliOrder Lepidoptera
The invention relates to the technical field of agriculture microorganism genetic engineering, in particular to separation and cloning of a Helicoverpa armigera cadherin gene fragment hacad1and encoded polypeptide fragment HaCad1. By separation, the cadherin gene fragment hacad1 of 735bp is obtained from the midintestine of the Helicoverpa armigera, an encoded product thereof is the polypeptide fragment HaCad1 which can be combined with Bt toxin CrylAc10. Biological experiments indicate the polypeptide prepared in the invention has very strong enhancing insecticidal activity on insecticidal crystal protein CrylAc10 of lepidoptera insects. The invention also includes preparation and an application for recombinant Escherichia coli EMB1104 expressing the Helicoverpa armigera cadherin fragment HaCad1. The recombinant Escherichia coli EMB1104 is preserved in CCTCC, the preservation number is CCTCC NO: M209010.
Owner:HUAZHONG AGRI UNIV
Pest control method for planting solanaceae crops in dragon fruit orchard
InactiveCN110476683AStimulation absorption ratePromotes nutrient absorptionBiocideExcrement fertilisersInsect pestBollworm
The invention relates to the technical field of pest control over dragon fruits, in particular to a pest control method for planting solanaceae crops in a dragon fruit orchard. According to the method, pretreated dragon fruit branches are subjected to cuttage around cement piles in the middle ten days and last ten days of March, then transplanting pretreatment is conducted among lines of the dragon fruits, peppers are transplanted at the two sides of the dragon fruits, field management is conducted, and after harvesting is completed, tomatoes are transplanted. According to the method, the dragon fruits, tomatoes and peppers are intercropped, the among-line space of the dragon fruits is sufficiently utilized, effective utilization of land of the dragon fruit orchard is achieved, the absorption rate of the dragon fruits is stimulated, and nutrient absorption by the dragon fruits is improved; through the cooperation of physiological properties of various fruits and vegetables, the growthof weeds and breeding of harmful microbes are inhibited, the physical and chemical properties of the soil are improved, nutrient loss of the soil is prevented, and the growth capacity of the crops isimproved; by treating the dragon fruits, the tomatoes and the peppers separately, the pest prevention capacity of the crops is improved, the occurrence rate of pests is lowered, and the pest damage ofaleyrodid, helicoverpa armigera and aphids is overcome.
Owner:GUIZHOU FRUIT INST
Composite insecticidal and acaricidal nano aqueous agent containing fenpropathrin and lambda-cyhalothrin, and uses thereof
InactiveCN110973153AWill not precipitateTransparent appearanceBiocideAnimal repellantsActive agentTetranychus cinnabarinus
The invention discloses a composite insecticidal and acaricidal nano aqueous agent containing fenpropathrin and lambda-cyhalothrin, wherein the composite insecticidal and acaricidal nano aqueous agentis prepared from active ingredients of fenpropathrin and lambda-cyhalothrin, a surfactant and water, the weight percentage content of the fenpropathrin and the lambda-cyhalothrin is 3-80%, the weightpercentage content of a pesticide adjuvant is 5-20%, the balance is water, and a weight ratio of fenpropathrin to lambda-cyhalothrin is (20:1)-(1:20). The insecticidal composition can generate high synergistic effect, overcomes and delays the drug resistance of pests, is high in insecticidal speed and long in lasting period, reduces the medication cost, has obviously high prevention and control effect compared with a single agent, can prevent and control lepidoptera pests on crops, especially can be used for preventing and controlling helicoverpa armigera and tetranychus cinnabarinus, and hassignificantly high effect compared with the use of a single agent.
Owner:NANJING HUAZHOU PHARMA
Benzamide or acylhydrazone derivative containing 5-trifluoromethyl-4-pyrazole amide structure, and application of derivative
InactiveCN103936674ABroad-spectrum high-efficiency insecticidal activityHigh insecticidal activityBiocideOrganic chemistryPhenyl groupMethyl group
The invention discloses an application of a benzamide or acylhydrazone derivative containing a 5-trifluoromethyl-4-pyrazole amide structure in the aspect of insecticidal activity. The general structure formulas are as shown in A and B in the specification, wherein R1 refers to phenyl and 3-chloropyridyl; R2-R5 refer to hydrogen atom, alkyl, hetroatom-containing alkyl, phenyl, benzyl, heterocyclyl and substituted heterocyclyl. The benzamide or acylhydrazone derivative has broad-spectrum insecticidal activity and has excellent preventing and controlling effects on diamondback moth, heliothis armigera, ostrinia nubilalis, armyworm and sanitary pests such as culex pipiens.
Owner:GUIZHOU UNIV
Recombinant expression method of insect C-type lectin and use
InactiveCN101434955AReduce fatalityReduce the number of survivorsPeptide/protein ingredientsAnimal feeding stuffVital activityEscherichia coli
The invention belongs to the technical field of bioengineering and provides a biological medicament that has recombinant gene expression of helicoverpa armigera C-type lectin and two carbohydrate recognizing structural domains of Ha-CRD1 and Ha-CRD2 of the gene. The helicoverpa armigera C-type lectin gene and the two carbohydrate recognizing structural domains of Ha-CRD1 and Ha-CRD2 of the gene have recombinant expression in Escherichia coli. The invention further provides a recombinant gene expression method of the helicoverpa armigera C-type lectin and the two carbohydrate recognizing structural domains of Ha-CRD1 and Ha-CRD2 of the gene. The lectin can reduce the insect fatal rate of pathogenic organisms, improve the pathogenic organism phagocytizing efficiency of blood corpuscle, and reduce the survival number of pathogenic organisms in insect bodies. The biological medicament does not generate drug-fast bacteria, does not have adverse effect to the normal vital activity of insects, can reduce the usage of antibiotic, and can reduce the drug-fast bacteria generation in environments.
Owner:SHANDONG UNIV
Method for preparing tung oil pesticide
InactiveCN106305803AExtend the industrial chainRich biological pesticide productsBiocideAnimal repellantsLeafhopperDrug resistance
The invention provides a method and process for preparing tung oil pesticide. The method comprises the following steps: refining tung oil with alkali, mixing with an emulsifier and uniformly mixing with other auxiliaries. The tung oil has effects of killing pests and inhibiting bacteria. The tung oil pesticide is simple in preparation, has a good pest killing effect and wide market application prospect, has the characteristics of fast action and long action, and can be used for reducing the usage of chemical pesticide and preventing pests from generating drug resistance. The tung oil pesticide does not have pollution in production and processing processes, has stable product quality, wide insecticidal spectrum, strong activity and convenience in use, and can be used for preventing and controlling beauveria bassiana, argyroneta aquatica, cabbage caterpillar, helicoverpa armigera, aphid, green leafhopper, cockroaches, acarid and the like.
Owner:王忠良 +1
Application of BZR1 gene in regulation and control of insect attack stress resistance of plants
The invention discloses application of a BZR1 gene in regulation and control of insect attack stress resistance of plants, and belongs to the technical field of biology. According to the application, the function of the BZR1 gene is deleted by utilizing a biotechnological means, so that the resistance of plants to insect attack stress is improved; and the coding sequence of the BZR1 gene is as shown in SEQ ID NO.1 or has at least 70% of homology with the sequence as shown in SEQ ID NO.1, and the coded protein is equivalent in function. The invention discloses the application of the BZR1 gene in regulating and controlling the insect attack stress, especially the helicoverpa armigera resistance of the plant for the first time, the function of the BZR1 gene is deleted through a gene editing technology, the helicoverpa armigera resistance of the plant can be remarkably enhanced, and the BZR1 gene can be applied to cultivation of insect attack stress resistant plant varieties.
Owner:ZHEJIANG UNIV +1
Pesticide composition containing Chromafenozide
InactiveCN106172402AGood synergyGood lasting effectBiocideDead animal preservationChlorfenapyrMatsumuraeses phaseoli
The invention discloses a pesticide composition containing Chromafenozide, and provides an insecticidal composition which comprises an active component A and an active component B, wherein the active component A is Chromafenozide, the active component B is anyone compound selected from the following components: Lufenuron, Ethiprole, Chlorfenapyr, and Pyriproxyfen, and the weight ratio between the active component A and the active component B is 1:70-70:1. The composition can be used for controlling a plurality of insects with obvious synergistic effects and enlarged insecticidal spectrum, and has high activities for rice hoppers, yellow rice borers, rice stem borers, rice leaf rollers, aphids, Empoasca pirisuga, Phthorimaea operculella, Laspeyresia pomonella, Carposina niponensis, Lithocolletis ringoniella, Matsumuraeses phaseoli, Phyllocnistis citrella, Eriophyes oleivorus, scale insects, Spodoptera exigua, Pieris rapae, Anterhynchium flavomarginatum, thrips, Spodoptera litura, Helicoverpa armigera, and Etiella zinckenella. Pesticide dosage, residual quantity of the pesticide on crops, and environment pollution are reduced.
Owner:陕西亿田丰作物科技有限公司
Medicinal plant insecticide and preparation method thereof
InactiveCN110250223AWide variety of sourcesPromote decompositionBiocideAnimal repellantsNicotiana tabacumPinellia
Owner:HUAIBEI NORMAL UNIVERSITY
Effective pesticide containing sulfoxaflor, flubendiamide, chlorpyrifos and biopesticide lepimectin
InactiveCN104255780ASynergistic effect is obviousReduce pollutionBiocideAnimal repellantsChlorpyrifosFlubendiamide
The invention relates to an effective pesticide composition for killing agricultural pests. The composition is prepared from the following components in parts by weight: 10-30 parts of sulfoxaflor, 15-55 parts of flubendiamide, 30-70 parts of chlorpyrifos and 35-65 parts of biopesticide lepimectin. The pesticide composition has a remarkable synergizing effect, can be safely applied to cabbages, tomatoes, oranges, strawberries, grapes and other vegetables and fruits, has a relatively broad insecticidal spectrum, has a very good dispelling and killing effect on most lepidopterous and homoptera pests, such as prodenia litura, plutella xylostella, helicoverpa armigera and mealybug, and has the characteristics of low residue, short half-life period, quick decomposition, small toxicity, high safety and the like.
Owner:QINGDAO RUNXIN WEIYE TECH & TRADE
Protein GmSqm associated with insect tolerance of plants as well as coding gene and application thereof
InactiveCN104628835AWorm weight reductionShorten body lengthPlant peptidesFermentationWild typeArabidopsis
The invention discloses a protein GmSqm associated with the insect tolerance of plants as well as a coding gene and application thereof. The protein is the following a) or b): a) a protein with an amino acid sequence shown in a sequence 1 in a sequence table; b) a protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence shown in the sequence 1 in the sequence table and is associated with the insect tolerance of plants. Experiments prove that compared with wild arabidopsis thaliana plants and trans-empty vector arabidopsis thaliana plants, trans-GmSqm arabidopsis thaliana plants have the advantages that after newly-hatched helicoverpa armigera Hubner larvae are fed with the trans-GmSqm arabidopsis thaliana plants, the weights of the helicoverpa armigera Hubner larvae are obviously reduced, and the body length of the helicoverpa armigera Hubner larvae is obviously reduced. The protein GmSqm is of great importance in cultivating plants tolerant to insects.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Method for obtaining large number of campoletis chorideae uchida wild populations
ActiveCN111869627AGuaranteed to parasitizeGood parasitic rateInsect catchers and killersBiotechnologyOrder Lepidoptera
The invention discloses a method for obtaining a large amount of campoletis chorideae uchida wild populations. The method uses lepidoptera larvae to be manually released before tobacco leaves or cornleaves grow vigorously to trap campoletis chorideae uchida, and comprises the following specific steps of slightly brushing the lepidoptera larvae on tender leaves of the tobacco leaves or the corn leaves by using a writing brush before the tobacco leaves or the corn leaves grow vigorously; and trapping the campoletis chorideae uchida to lay eggs on the lepidoptera larvae, and manually collectingwild population cocoons of the campoletis chorideae uchida within a fixed time limit. The method further comprises the step that attractants are smeared on the tender leaves of the tobacco leaves or the corn leaves at the same time, and inducers are one or a mixture of more of host plant volatile matter, nectariferous plant extracts and parasitic information gland compounds. Compared with the prior art, the method for obtaining a large number of campoletis chorideae uchida wild populations provided by the invention can obtain a large number of populations within the fixed time limit, and is used for indoor propagation and rejuvenation.
Owner:HUNAN ACAD OF FORESTRY +1
Plant-derived self-emulsifying nano-insecticide as well as preparation method and application thereof
InactiveCN108184908AImprove biological activityImprove efficiencyBiocideAnimal repellantsActive agentMass ratio
The invention discloses a plant-derived self-emulsifying nano-insecticide as well as a preparation method and application thereof. The plant-derived self-emulsifying nano-insecticide is prepared fromthe following components: 2-5% of crude ethanol extract from ginkgo biloba exocarp, 2.5-3% of surface active agent and 2-2.5% of cosurfactant; the mass ratio of a solvent-ethanol to a ginkgo biloba exocarp crude extract is equal to 1 to 1. The application objects of the nano-insecticide are pests including lepidoptera plutella xylostella, armyworm, helicoverpa armigera, and the like. The plant-derived self-emulsifying nano-insecticide has the advantages that anhydrous self-emulsifying nanoemulsion is introduced into pesticide by adopting the natural surface active agent, so that the biologicalactivity of the natural product-derived pesticide can be effectively improved, and the production quality and yield are better improved; furthermore, the plant-derived self-emulsifying nano-insecticide reduces the production cost, has a control effect of higher than 90% for all the pests, and also has a control effect of higher than 90% for aphids.
Owner:HUNAN UNIV OF SCI & ENG
Applications of 3,4-dimethylbenzaldehyde
PendingCN110742070AGood lure effectRealize pollution-free prevention and controlBiocidePest attractantsSecondary metaboliteEcology
The invention discloses applications of 3,4-dimethylbenzaldehyde, particularly applications of the 3,4-dimethylbenzaldehyde in preparation of pest attractants, wherein the 3,4-dimethylbenzaldehyde isone of volatile secondary metabolites synthesized by Chinese rose plants. According to the invention, the active component is separated from the Chinese rose headspace volatile matter, and the results of GC-EAD, EAG, olfactory behavior, oviposition behavior biological tests and field oviposition tests prove that the 3,4-dimethylbenzaldehyde has good attracting effect on pests, especially Helicoverpa armigera female moths; and the 3,4-dimethylbenzaldehyde is a natural secondary metabolite and has a complete degradation path in nature, so that the 3,4-dimethylbenzaldehyde is used as a main component to prepare the pest attractant so as to achieve the pollution-free prevention and control of pests.
Owner:SOUTHWEST FORESTRY UNIVERSITY
Nitrogen-containing bridged ring compound having insect disinfestation activity, preparation and uses thereof
ActiveCN105461724AHigh insecticidal activityExpand insecticidal spectrumBiocideOrganic chemistryHomopteraOrder Lepidoptera
The present invention relates to a nitrogen-containing bridged ring compound having insect disinfestation activity, preparation and uses thereof, and particularly provides a compound having a structure represented by a general formula I, a preparation method and uses thereof, wherein R1, R2, R3, R4, R5, R6, R7, R8, R, n and Z are defined in the specification. The present invention further relates to an agricultural composition containing the compound or a pesticide acceptable salt, and uses of the agricultural composition. According to the present invention, the compound provides high insect disinfestation activity on Homoptera pests, Lepidoptera pests and other agricultural and forestry pests such as aphid, planthopper, whiteflies, leafhoppers, thrips, Helicoverpa armigera Hubner, Pieris rapae, diamondback moth, Spodoptera litura, armyworms and the like. The general formula I is defined in the specification.
Owner:EAST CHINA UNIV OF SCI & TECH
Juvenile hormone transmethylase genes and method of using the same
Juvenile hormone acid methyltransferase gene was cloned using a cDNA derived from the corpora allata of Bombyx mori by differential display methods. A recombinant protein expressed in Escherichia coli, which was transformed with a vector DNA incorporating the gene, was found to have juvenile hormone acid methyltransferase activity. Based on amino acid sequence homology, juvenile hormone acid methyltransferase genes were found from Drosophila melanogaster, Anopheles gambiae, Spodoptera litura, and Helicoverpa armigera. The proteins encoded by the juvenile hormone acid methyltransferase genes derived from Drosophila melanogaster, Spodoptera litura, and Helicoverpa armigera were found to have juvenile hormone acid methyltransferase activities.
Owner:INC ADMINISTRATIVE AGENCY NAT AGRI & BIO ORIENTED RES ORG
Insecticidal agent, method for the preparation thereof and a method for controlling insects
InactiveUS20070059330A1Effective controlHeavy metal active ingredientsBiocideAdhesiveOrder Lepidoptera
The present invention provides an insecticidal agent useful for the controlling the insect pests belonging to the order Lepidoptera viz. Helicoverpa armigera, Pectinophora gossypiella, Spodoptera litura, or Earias vitella or mixed populations thereof on different crops comprising inorganic molecular sieve dissolved in water and naturally occurring inert adhesive such as gum arabica or any other commercially available inert sticker. It also deals with an effective method for controlling the said insect pests using said insecticidal agent. Because of very high activity, low application rates of these compounds are employed for maintaining the control of the pest. These relatively low application rates, together with practically no mammalian toxicity possessed by the compounds of the present invention makes them more effective as well as eco-friendly.
Owner:COUNCIL OF SCI & IND RES
Synergistic pesticide composition containing lambda-cyhalothrin and hexaflumuron
InactiveCN106719780AReduce dosageSynergistic effect is obviousBiocideDead animal preservationAdditive ingredientManduca quinquemaculata
The invention relates to a synergistic pesticide composition containing lambda-cyhalothrin and hexaflumuron. The effective ingredient of the synergistic pesticide composition is a binary combination of the lambda-cyhalothrin and the hexaflumuron, wherein the mass ratio of the lambda-cyhalothrin to the hexaflumuron is (1:50) to (50:1), and the mass ratio of the effective ingredients, namely the lambda-cyhalothrin and the hexaflumuron in a preparation is 1-80%, and the balance is an auxiliary ingredient. The dosage form of the pesticide composition is water dispersible granules, wettable powder, a suspending agent, missible oil,a microemulsion or an emulsion in water. The synergistic pesticide composition is mainly used for preventing and controlling lepidoptera pests, such as helicoverpa armigera, heliothis virescens, tobacco hornworm, diamond back moth, armyworm, beet armyworm, spodoptera frugiperda, cabbage argyrogramma agnate, imported cabbageworm, flowering cabbage, cabbage strip moth, manduca quinquemaculata, rice-stem borer, rice leaf folder and ostrinia nubilalis. The synergistic pesticide composition has a significant synergistic effect, the insecticidal activity is obviously strengthened in comparison with that of a single dosage, the dose of a pesticide is reduced and the environmental pollution is reduced.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Pesticide composition for preventing and controlling cotton field helicoverpa armigera and application thereof
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Streptomyces with double functions of preventing diseases and killing pests, application and culture method thereof, and biocontrol agent
The invention relates to streptomyces with double functions of preventing diseases and killing pests, an application and a culture method thereof, and a biocontrol agent thereof, and belongs to the technical field of plant pest control. In the invention, an endophytic streptomyces MS001 with bacteriostatic activity is separated and obtained. The fermentation liquor has stronger bacteriostatic activity on Botryosphaeria dothidea, Colletotrichum gloeosporioides, Phomopsis mali, Rhizoctonia cerealis, Gaeumannomyces graminis, Botrytis cinerea, Fusarium graminearum, Fusarium oxysporum and Colletotrichum karstii, and the bacteriostatic objects have broad-spectrum property; and the fermentation liquor has the function of inhibiting the growth of plant pests such as helicoverpa armigera and monochamus alternatus larvae. Therefore, the streptomyces MS001 can prevent and control some diseases and pests on apple trees, garden plants, vegetables and food crops.
Owner:HENAN UNIV OF SCI & TECH
Application of abc transporter as a target in pest control
The invention provides an application of an ABC transporter as a target to pest control. Gene ABCB6 of Helicoverpa armigera is knocked out with a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeat / CRISPR-associated nuclease 9) gene editing technology, the sequence of the gene ABCB6 is determined with an RACE technology and existing transcriptome data, a sgRNA target site is designed according to the sequence, after in-vitro transcription of sgRNA, sgRNA and Cas9 protein are mixed proportionally and injected into Helicoverpa armigera eggs, knockout of the gene ABCB6 succeeds,and a 7bp-deleted homozygous knockout strain is obtained through two-generation hybrid screening and purification. A detection result shows that weight gain of the knockout strain in treatment with gossypol concentration being 0.4% is inhibited seriously, meanwhile, sensibility to indoxacarb is improved, and LC50 is reduced 3.3 times. The results prove that the gene ABCB6 plays an important rolein indoxacarb and gossypol metabolism of the Helicoverpa armigera, and the new target gene is provided for controlling the Helicoverpa armigera.
Owner:AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
A protein gmifr related to plant insect resistance and its coding gene and application
The invention discloses a protein GmIFR associated with the insect tolerance of plants as well as a coding gene and application thereof. The protein is the following a) or b): a) a protein with an amino acid sequence shown in a sequence 1 in a sequence table; b) a protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence shown in the sequence 1 in the sequence table and is associated with the insect tolerance of plants. Experiments prove that compared with wild arabidopsis thaliana plants and trans-empty vector arabidopsis thaliana plants, trans-GmIFR arabidopsis thaliana plants have the advantages that after newly-hatched helicoverpa armigera Hubner larvae are fed with the trans-GmIFR arabidopsis thaliana plants, the weights of the helicoverpa armigera Hubner larvae are obviously reduced, and the body length of the helicoverpa armigera Hubner larvae is obviously reduced. The protein GmIFR is of great importance in cultivating plants tolerant to insects.
Owner:OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
Method for improving biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs)
InactiveCN103814950BIncreased sensitivityShorten the time to deathBiocideAnimal repellantsInclusion bodiesNuclear Polyhedrosis Virus
The invention belongs to the technical field of biological Helicoverpa armigera prevention and control, and particularly relates to a method for improving the biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs). The method can improve the Helicoverpa armigera prevention and control effect of NPVs through the feeding of a Helicoverpa armigera C-type lectin dsRNA (double-stranded ribonucleic acid) (Ha-lectin1 dsRNA); the Helicoverpa armigera prevention and control effect of NPVs can be remarkably improved at a dosage of 3 micrograms of Ha-lectin1 dsRNA and 50 PIBs (polyhedral inclusion bodies) of NPVs per larva of Helicoverpa armigera. The method provided by the invention has a fast onset of insect killing, that is, the time of Helicoverpa armigera death caused by Helicoverpa armigera NPVs (HaNPVs) can be shortened by 1 / 3; the insect killing efficiency is high, that is, the killing rate in the first three days can be increased by 35 percent; the consumption is low, that is, the use consumption of NPVs can be greatly reduced. Compared with the method which simply uses NPVs for Helicoverpa armigera prevention and control in the prior art, the method provided by the invention achieves remarkable improvements.
Owner:HENAN UNIVERSITY
Phenylacrylamide compounds with antifeedant effect on cotton bollworm in Solanum echinacea and its extraction and separation method and application
ActiveCN110256276BReveal the mechanism of intrusionTurn waste into treasureBiocideOrganic chemistry methodsSolanum sarrachoidesEthyl acetate
The invention belongs to the technical field of agriculture, and in particular relates to phenylacrylamide compounds. The extraction and separation method is as follows: take dried Solanum solanum and then concentrate under reduced pressure to obtain the extract; after suspending in distilled water, extract with petroleum ether and ethyl acetate respectively, and concentrate under reduced pressure to obtain the extract; Chromatography, eluting, collecting fractions, and silica gel thin-layer chromatography, to obtain four parts Fr.A ~ Fr.D; Fr.B is eluted by silica gel column chromatography, collecting fractions, silica gel thin-layer chromatography, Get Fr.B-1~Fr.B-6 six parts; .B-2-5 five parts; using high performance liquid chromatography, in Fr.B-2-2 ~ Fr.B-2-4 separation to obtain the target product. The extraction and separation method of the invention is simple, has good reproducibility and high extraction purity. The obtained compound has good antifeedant activity to cotton bollworm.
Owner:SHENYANG AGRI UNIV
Insecticidal composition containing methoxyfenozide and synthetic pyrethroid insecticide
ActiveCN101919386BImprove securityMeet safety requirementsBiocideAnimal repellantsSuspending AgentsToxicology
The invention discloses an insecticidal composition containing methoxyfenozide and etofenprox, bifenthrin or lambda-cyhalothrin synthetic pyrethroid insecticide. The weight ratio of the methoxyfenozide to the synthetic pyrethroid insecticide in the composition is 40:1-1:10, preferably 20:1-1:5. The insecticidal composition can be prepared into water dispersible granules, wettable powder, suspending agent, suspending emulsion, dispersible suspending agent, cream, microemulsion or microcapsule suspending agent. The insecticidal composition is used for preventing and controlling Chilo suppressalis, Tryporyza incertulas (walker), Cnaphalocrocis medialis Guenee, Helibthis armigera Hiibner, Adoxophyes orana and Laphygma exigua Hubner, and has the advantages of reasonable components, good insecticidal effect and low administration cost; and the activity and the insecticidal effect of the insecticidal composition are not simple superposition of the activities of the components but remarkable synergy, and the insecticidal composition can retard the generation of resistant, has good safety for crops and meets the safety requirement of pesticide preparations.
Owner:SHAANXI SUNGER ROAD BIO SCI
A compound insecticidal composition containing lepimectin and diflubenzuron and its application
ActiveCN105941445BOvercoming and delaying drug resistanceIncrease resistanceBiocideDead animal preservationChlorfenapyrOrder Lepidoptera
The invention discloses a compound insecticidal composition containing Lepimectin and chlorbenzuron and application thereof. In the insecticidal composition, Lepimectin and chlorbenzuron are insecticidal active ingredients, wherein the weight ratio of the Lepimectin to chlorbenzuron is (0.1-80):(0.5-80). The insecticidal composition can generate a relatively strong synergistic effect, and can be used for overcoming and delaying the pest resistance to insecticide, realizing high insecticidal speed and long duration and reducing the application cost while the control effect is obviously better than that of single dosage. The compound insecticidal composition disclosed by the invention can control the lepidoptera pests on crops and particularly can be used for controlling helicoverpa armigera and borer while the effect is obviously better than that of single dosage.
Owner:ANHUI RUIBANG BIOLOGICAL SCI & TECH CO LTD
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