31 results about "Hypoxic stress" patented technology

The reagent, pharmaceutical formulation, kit, and methods of the invention provides a new approach to alleviate or eliminate certain negative effects associated with the use of certain cancer treatment agents (e.g. chemotherapy therapeutics, etc.) or regimens (e.g. radio therapies, etc.), including stimulation of the hypoxic stress response in tumor cells.

The present invention provides compositions and methods for resolving bioavailable oxygen supply to plants subjected to hypoxic stresses. Compositions of the invention comprise an oxidizing agent, wherein the level and rate of oxygen released from the composition is controlled. Use of the compositions of the invention address hypoxic stress and also stimulate plant growth, enhance plant vigor, and / or improve crop yield.

The reagent, pharmaceutical formulation, kit, and methods of the invention provides a new approach to alleviate or eliminate certain negative effects associated with the use of certain cancer treatment agents (e.g. chemotherapy therapeutics, etc.) or regimens (e.g. radio therapies, etc.), including stimulation of the hypoxic stress response in tumor cells. The reagent and pharmaceutical formulation of the invention relates to Na+ / K+-ATPase inhibitors, such as cardiac glycosides, including bufadieneolides or their corresponding aglycones (e.g., proscillaridin, scillaren, and scillarenin, etc.), especially in oral formulations and / or solid dosage forms containing more than 1 mg of active ingredients.

The invention discloses an aeration treating method of tobacco floating seedling. The aeration treating method comprises the following steps of: before seeding and panning, mixing calcium peroxide with matrix according to the using amount of 150.0 to 450.0g / m<3> evenly, adding the calcium peroxide with the concentration of 0.09 to 0.27g / L into pond culture solution when the second true leaf and the sixth true leaf respectively grow out of the tobacco seedling, and other operations are respectively conventional technique. The aeration treating method can solve the hypoxic stress of floating seedling to tobacco seedling, improve the environment for the sprouting of tobacco seeds and growing of the root system of the tobacco seedling, improve the uniformity of seedling emergence, strengthen the activity of the root system of the tobacco seedling, enhance the growth of the tobacco seedling, improve the quality of the tobacco seedling, and is beneficial to culturing disease-free strong tobacco seedlings. The tobacco seedling cultured by adopting the method has the advantages of short seedling emergence time, high transplanting survival rate, strong field growth potential and the like. The invention has simple and convenient operation, low cost and good application prospect.

Sickle cell patients atypically experience exaggerated inflammatory responses to pathogens that normally cause mild respiratory infections in non-sickle cell humans. There appears to be heightened inflammatory responses to pathogens in combination with hypoxia in sickle cell disease. The novelty of this invention provides a new paradigm to explain the exaggerated inflammatory response of sickle cell disease to pathogens especially when accompanied by hypoxic stress. In particular, sickle cell chest injury and other complications associated with ischemia-reperfusion injury caused by vaso-occlusion can involve co-stimulation of the NALP-3 inflammasome by pathogen associated molecular patterns (PAMPs) and hypoxic-induced danger associated molecular patterns (DAMPs), leading to exaggerated pro-inflammatory responses marked by increased IL-1β secretion and subsequent induction of neutrophilic inflammation. This invention thereby provides the immunologic, biologic and biochemical rationale for IL-1β targeted therapies in sickle cell disease to block the pathological effects of IL-1β that leads to exaggerated inflammatory expressions, including neutrophilic inflammation.

The invention discloses an early hypoxia detection kit using miRNA-210 as a marker, which belongs to the technical field of molecular biomedicine. The present invention designs miRNA-210-specific primers, and uses quantitative PCR to detect changes in the content of miRNA-210 in rat cerebrospinal fluid, blood, brain tissue, neural stem cells, and neural stem cell culture fluid in different concentrations of hypoxic environments, and the content is significant. Increased for hypoxic stress tissue. The method of the invention is simple, rapid and sensitive, and is suitable for detecting whether animals and cells are in hypoxic state.

A hypoxic training facility includes a plurality of walls defining an enclosure for housing an animal to be trained. A hypoxicator is adapted for delivering hypoxic, hyperoxic, and normoxic air to the enclosure during a hypoxic training session. An animal exercise station is located within the enclosure. A pulse oximeter monitors oxygen saturation of the animal's blood during the hypoxic training session. A computing device calculates hypoxic stress delivered to the animal during the hypoxic training session.

An object of this invention is to produce a protein by efficiently culturing plant cells while escaping mRNA translational repression in cultured plant cells under stress caused by lack of conditions essentially required for the growth (e.g., nutrient-starvation stress and hypoxic stress). This invention provides a method for escaping translational repression of a protein encoded by mRNA, the repression being induced by stress due to absence of conditions required for the growth, the method comprising the step of culturing a plant cell transformed with a recombinant DNA molecule encoding mRNA containing 5′ UTR defined in (a) or (b) below, the culturing being carried out under stress due to absence of conditions required for the growth: (a) 5′ UTR having a base sequence of SEQ ID NO: 1, 2, 3, 4, 5, or 6; or (b) 5′ UTR having the base sequence of the 5′ UTR of (a) in which one or more bases are replaced, deleted, or added, and which escapes translational repression induced by stress due to absence of conditions required for the growth.

The present invention provides a use of a HIF-2alpha inhibitor in preparation of drugs for preventing and treating acute high-altitude reaction. The HIF-2alpha inhibitor comprises HIF-2alpha-I2 and / orPT2385; the use according to the invention fully investigates molecular mechanisms and key molecular events and processes of acute high-altitude reaction and hypoxia stress, and a series of experiments shows that HIF-2alpha-I2 and PT2385 both can significantly improve the malignant degree and symptoms including slowing shortness of breath, emotional upset, and high heart rate of acute high-altitude reaction, as well as alleviate the acute hypoxic stress response, so as to achieve the purpose of effectively preventing acute hypoxia. The use has good safety, little side effects and other advantages, and has a good application prospect.

The present invention provides a use of soluble P-selectin in treating systemic hemorrhagic conditions, stabilizing blood pressure, and protecting hypoxic / ischemic tissues. Also provided is a use of anthrax lethal toxin in treating thrombotic conditions.

The invention discloses a digital simulator for AMS risk assessment. The digital simulator comprises a crowd simulation module, a measurement interference simulation module and an AMS risk assessment module. The crowd simulation module is used for creating simulation crowds with different hypoxia tolerance capacities by applying a mathematical model of a human hypoxia stress system and a plurality of instance experiment data. the measurement interference simulation module is used for simulating measurement interference and deep respiration physiological interference in the SpO2 information, and superposing simulated interference signals onto the SpO2 generation curve of the simulated crowd; and the AMS risk assessment module obtains indexes used for assessing AMS risks, and the indexes are designed based on parameters of the identified model and used for representing the real hypoxia adaptability of different virtual crowds. According to the AMS risk assessment method, richer research data can be obtained for AMS risk assessment algorithm research, and effective and reliable AMS risk assessment is realized.

The invention discloses SNP (single nucleotide polymorphism) molecular markers related to hypoxia tolerance of rice and application of the SNP molecular markers. The SNP molecular markers provided bythe invention are located at the 30th, 096th and 391st sites of rice chromosome 3, and the polymorphism is A or G. The SNP molecular markers are significantly correlated with the survival rate of riceseedlings under hypoxic stress conditions. A rice variety with a genotype being GG at the sites is significantly stronger in resistance to hypoxic germination than that of a rice variety with the genotype being AA under hypoxic stress conditions. The invention also designs PCR primers based on the SNP sites, and the nucleotide sequences of the primers are shown in sequence 1 and sequence 2. The SNP molecular markers related to the hypoxia tolerance in rice provided by the invention are verified in natural populations that the markers can effectively predict the hypoxia germination tolerance of rice, and thus can be used for marker-assisted breeding of hypoxia tolerance and direct seeding molecular breeding in rice.

Persons with sleep disordered breathing (SDB) may, or may not, recognize that they have symptoms of SDB, and / or that they may be at-risk of, or suffering certain health problems associated with SDB, including death. The disclosed Energy Conversion Monitor (ECM) sensor, when embodied, for example, in a wearable upper-armband format, has been demonstrated to be more sensitive and responsive than pulse oximetry monitoring of blood oxygen saturation as an indication of hypoxic stress induced by SDB, and is compatible with: (1) inclusion in sleep laboratory polysomnograph (PSG) testing instrumentation, (2) home-based diagnostic testing for SDB, (3) control of home-use airway therapy devices, (4) continuous remote surveillance and refinement of airway therapy, and (5) spot-check and continuous surveillance of sleep quality in the general population. The disclosed ECM also provides new measurements of physiologic stress during and following exercise. When applied during initial care of premature newborn infants, it offers improved therapeutic guidance during their transition from their limited in utero oxygen supply conditions, to the increased oxygen availability from breathing air. When applied during resuscitation of persons suffering from hypoxia and during reperfusion of ischemic tissue, such as during treatment of ischemic stroke, or ischemic heart attack, the ECM sensor can provide objective guidance regarding the safe and effective resupply of oxygen to the hypoxia-adapted tissue to help reduce or prevent microvascular occlusion and cellular injury. As a continuously worn physiologic surveillance monitor, the ECM offers the potential of early detection of sepsis. For the elderly and infirm, the ECM offers a convenient and comfortable means of continuously assessing variations in status while awake and asleep.

The present invention provides a use of soluble P-selectin in treating systemic hemorrhagic conditions, stabilizing blood pressure, and protecting hypoxic / ischemic tissues. Also provided is a use of anthrax lethal toxin in treating thrombotic conditions.

The invention discloses a SNP molecular marker related to hypoxia resistance traits of mandarin fish and its application, including molecular marker SNP1 and molecular marker SNP2; the molecular marker SNP1 is located at the 5' from the nucleotide sequence shown in SEQ ID NO.1 The 1919th position from the end, where the base is T or C; the molecular marker SNP2 is located at the 4784th position from the 5' end of the nucleotide sequence shown in SEQ ID NO.1, where the base is T or C. The SNP loci disclosed in the present invention are significantly related to the survival rate of mandarin fish under hypoxic stress. Individuals of the SNP 1919 TT genotype and 4784 TC genotype have significantly stronger hypoxia tolerance than individuals with TC and TT genotypes. Molecular marker-assisted breeding can be carried out without restrictions on the age and sex of mandarin fish. It can be used for early breeding of mandarin fish, which can significantly promote the breeding process of mandarin fish and the survival rate under low oxygen conditions during transportation. The method is accurate and reliable. Easy to operate.

This invention relates to the use of free L-proline in the preparation of a supplement, and to a supplement so prepared, as well as a method using such a supplement for supplementing the concentration of cellular free L-proline in abalone and for restricting the dehydration of abalone. The supplement is further effective in reducing the levels of free radicals in abalone experiencing water stress and hypoxic stress. The invention further relates to a method of restricting the dehydration of abalone during storage and transportation including the step of administering to abalone an effective amount of free L-proline.

The invention relates to a carassius auratus hypoxia-inducible factor HIF-1alpha gene. The carassius auratus hypoxia-inducible factor HIF-1alpha gene has a nucleotide sequence of SEQ ID NO. 1. The invention discloses a nucleotide sequence and an amino acid sequence of the carassius auratus hypoxia-inducible factor HIF-1alpha gene for the first time; the carassius auratus hypoxia-inducible factor HIF-1alpha gene is verified to exert effects in hypoxic stress of carassius auratus through a qPCR technology; the expression mode of carassius auratus hypoxia-inducible factor HIF-1alpha in liver, brain, heart and gill tissues is analyzed by utilizing real-time fluorescence quantitative qPCR; and results show that the expression modes of HIF-1alpha genes in the liver, brain, heart and gill tissuesof the carassius auratus are different under the condition of hypoxia stress, and basic data and a reference can be provided for a fish hypoxia adaptation mechanism, a hypoxia signal transduction path and cultivation of novel hypoxia-resistant fish varieties.

The invention discloses an aeration treating method of tobacco floating seedling. The aeration treating method comprises the following steps of: before seeding and panning, mixing calcium peroxide with matrix according to the using amount of 150.0 to 450.0g / m<3> evenly, adding the calcium peroxide with the concentration of 0.09 to 0.27g / L into pond culture solution when the second true leaf and the sixth true leaf respectively grow out of the tobacco seedling, and other operations are respectively conventional technique. The aeration treating method can solve the hypoxic stress of floating seedling to tobacco seedling, improve the environment for the sprouting of tobacco seeds and growing of the root system of the tobacco seedling, improve the uniformity of seedling emergence, strengthen the activity of the root system of the tobacco seedling, enhance the growth of the tobacco seedling, improve the quality of the tobacco seedling, and is beneficial to culturing disease-free strong tobacco seedlings. The tobacco seedling cultured by adopting the method has the advantages of short seedling emergence time, high transplanting survival rate, strong field growth potential and the like. The invention has simple and convenient operation, low cost and good application prospect.

Provided are methods for inhibiting cancer cell growth comprising contacting the cancer cell with an agent which inhibits the expression of the gene, or the activity of, apolipoprotein B editing catalytic 3G (APOBEC3G). Also provided are methods for identifying agents which can induce or inhibit C>U deamination in RNA driven by apolipoprotein B editing catalytic proteins. The method comprises contacting APOBEC3G with a suitable RNA substrate and determining the extent of C>U deamination under conditions which induce APOBEC driven C>U deamination.

The invention discloses a method for breeding low-oxygen tolerant lineages of Ussuri catfish, which comprises the steps of parent selection, induction of labor, insemination, hatching and fry cultivation, low-oxygen stress and survival rate calculation, and the like. The present invention selects the parents with well-developed glands, and under the action of the oxytocin drug at an appropriate ratio and dose, can achieve a better oxytocin effect; secondly, each female fish is subjected to egg squeezing, insemination, egg distribution, hatching and frying separately. Cultivation and other operations, so as to construct the Ussuri catfish family; finally, through effective hypoxic stress treatment, the hypoxia-tolerant family was bred. The Pseudomonas Ussuri family line construction and the low-oxygen-tolerant family breeding technology provided by the present invention will provide technical support for the artificial breeding of Pseudomonas Ussuri based on multiple lines, and will also speed up the development of low-oxygen tolerance of Pseudomonas Ussuri. The breeding process of new oxygen varieties is of great significance.

The present invention provides the application of miRNA708 and / or microRNA of cluster 301 in improving heart function. Specifically, microRNAs of miRNA708 and / or 301 clusters can (i) promote cardiomyocyte proliferation; (ii) improve cardiomyocyte anti-hypoxic stress ability; (iii) reduce cardiomyocyte apoptosis and death; (iv) induce myocardial cell proliferation; Proliferation and differentiation of stem cells; and / or (v) protecting cardiomyocytes from hypoxic stimulation.

The invention discloses a digital simulator for AMS risk assessment, comprising a crowd simulation module, a measurement interference simulation module and an AMS risk assessment module; data, creating simulated populations with different hypoxic tolerance; measurement interference simulation module for simulating SpO 2 The measurement interference and deep breathing physiological interference in the information, and the simulated interference signal is superimposed on the SpO2 of the simulated population 2 On the generated curve; the AMS risk assessment module acquires indicators for assessing AMS risks, which are designed based on the parameters of the identified model and are used to characterize the real hypoxic adaptability of different virtual populations; the present invention can be used for AMS risk assessment algorithm research to obtain Richer research data and effective and reliable AMS risk assessment.

The invention provides a compound preparation with anti-hypoxia stress function, which is mainly used for preventing and treating various pathological or physiological states caused by hypoxic injuries and belongs to the field of medicine. The compound preparation with anti-hypoxia stress function is an oral preparation prepared from valerian extract, luteolin, camomile flower extract, conjugated linoleic acid and ginseng extract used as active pharmaceutical ingredients by using pharmaceutically conventional adjuvants and technique. The animal experiment indicates the anti-hypoxia compound preparation has favorable hypoxia resistance, and can effectively improve various hypoxic pathological states caused by lower oxygen content in the external environment. After the preparation is used by nearly 300 people, including soldiers rushing into the plateau, in-plateau troops and tourists and working personnel entering the plateau, the field experiment data indicates that the compound preparation can effectively the mental work capacity and physical endurance of the human body in a hypoxia environment.

This invention relates to the use of free L-proline in the preparation of a supplement, and to a supplement so prepared, as well as a method using such a supplement for supplementing the concentration of cellular free L-proline in abalone and for restricting the dehydration of abalone. The supplement is further effective in reducing the levels of free radicals in abalone experiencing water stress and hypoxic stress. The invention further relates to a method of restricting the dehydration of abalone during storage and transportation including the step of administering to abalone an effective amount of free L-proline.

The invention discloses a method for determining whether a tilapia is in a hypoxia stress state or not. The method comprises the steps of measuring expression quantity of a GABA transport protein 2 gene of a spleen of a normal tilapia and that of a spleen of a to-be-measured hypoxia-processed tilapia separately; if the expression quantity of the GABA transport protein 2 gene of the spleen of the to-be-measured hypoxia-processed tilapia is obviously lower than that of the spleen of the normal tilapia, representing that the to-be-measured hypoxia-processed tilapia is in the hypoxia stress state; and if obvious difference does not exist between the two sides, representing that the to-be-measured hypoxia-processed tilapia is not in the hypoxia stress state. Experiments prove that a specific primer used for detecting the GABA transport protein 2 gene of the spleen of the tilapia has high specificity and sensitivity and high amplification efficiency, and can be used for detecting the level of the expression efficiency of the GABA transport protein 2 gene.

The invention belongs to the technical field of sprouted grains and relates to an industrial production method of sprouted millet. Shelled millet are placed in a sprouting device; hypoxic stressed sprouting is carried out at 0-40 DEG C for 21-32.5 h; an accelerating liquid, a regulating agent and a bactericide are sprayed to the sprouting device at intervals of 30-40 min; after sprouting, cleaningand drying are performed to obtain sprouted millet. The method of producing sprouted millet is initiated herein; efficient high-quality sprouting is achieved for millet; the grown sprouted millet hassprouts reaching 1-2 mm in length; nutritional value and bioactivity of millet are greatly improved; a new chapter of sprouted grains is explored.