55 results about "Malic enzyme" patented technology

A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

The invention discloses a method for reducing NAD analogues by utilizing methanol and applications. In the method, a reducing agent is the methanol, a catalyst is the methanol dehydrogenase capable ofutilizing the methanol, and NAD analogues can be converted into the reduced states of the NAD analogues while the methanol dehydrogenase oxidizes the methanol; the method can be used for producing the reduced state NAD analogues or the reduced state analogues of deuteration and can provide reduced state coenzymes for the enzymatic reaction consuming the reduced state NAD analogues; the reduced state NAD analogues can be used as coenzymes to be applied to the reduction reaction catalyzed by enzymes such as malic enzyme ME-L310R/Q401C, D-lactic dehydrogenase DLDH-V152R and saccharomyces cerevisiae alcohol dehydrogenase, so that the wide application of the NAD analogues can be realized; the reduction method of the NAD analogues can be performed under a mild condition; and the regenerated reduced state NAD analogues can be used for preparing malic acid or lactic acid and can be used as redox power to control and regulate the metabolic intensity of the malic acid or lactic acid in microorganisms.