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40 results about "Paromomycin" patented technology
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This medication is used to treat a certain parasite infection of the intestines (amebiasis).
Tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof
InactiveCN101586119AImprove relevanceIncrease credibilityMicrobiological testing/measurementChemiluminescene/bioluminescenceGene vectorBiology
The invention discloses a tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof. Plasmid T-GFP is constructed by using plasmid rDNA-GFP in the methods such as enzyme cutting and PCR enzyme cutting site mutation; thermophile tetrahymena genome DNA is used for constructing plasmid T-HSP702 in the methods such as PCR augmentation and clone; Plasmids T-GFP, T-HSP702 and pD5H8 are used for construction and finally obtaining plasmid HSP702-GFP-pD5H8 by enzyme cutting and connection. The carrier contains HSP702 promoter, thereby being capable of realizing high-efficient expression of exogenous gene; the carrier contains replaceable exogenous gene gfp so that an exogenous gene expression system is realized for tetrahymena by replacing different exogenous genes; with the sieving effect of paromomycin medicine, the carrier replaces most rDNAs originally in the tetrahymena so that the exogenous genes are largely increased in the tetrahymena so as to realize genetic transformation of target genes. The method in the invention is easy and has convenient operation, and the carrier can be used for detection of environmental tributyl tin in transfection in tetrahymena.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Antimicrobial 2-deoxystreptamine compounds
The present invention is directed to analogs of paromomycin having a variety of chemical functional groups attached at the 2″-O-position as well as their preparation and use as prophylactic or therapeutics against microbial infection.
Owner:IONIS PHARMA INC
Novel aminoglycosides and uses thereof in the treatment of genetic disorders
ActiveUS20090093418A1Improved termination mutation suppression activityLow toxicityBiocideMuscular disorderChemo enzymaticInosamycins
A new class of paromomycin-derived aminoglycosides, which exhibit efficient stop-codon mutation suppression activity, low toxicity and high selectivity towards eukaryotic cells are provided. Also provided are chemical and chemo-enzymatic processes of preparing these paromomycin-derived aminoglycosides and intermediates thereof, as well as pharmaceutical compositions containing the same, and uses thereof in the treatment of genetic disorders.
Owner:TECHNION RES & DEV FOUND LTD
Crystal structure of antibiotics bound to the 30S ribosome and its use
InactiveUS7079956B2High resolutionMicrobiological testing/measurementAnalogue computers for chemical processesHygromycin BX-ray
The invention provides high resolution X-ray crystal structures of the 30S ribosome, obtained from Thermus thermophilus 30S subunit, having a tetragonal space group P41212 to which are bound an antibiotic selected from the group paromomycin, streptomycin, spectinomycin, tetracycline, pactamycin and hygromycin B. An advantageous feature of the structure is that it diffracts at about 3 Å resolution. The invention also provides a crystal of 30S having the three dimensional atomic coordinates of the 30S ribosome, the coordinates being provided in any one of tables 1 to 4. The data may be used for the rational design and modelling of inhibitors for the 30S ribosome, which have potential use as antibiotics.
Owner:UK RES & INNOVATION LTD
Monoclonal antibody, enzyme-linked immunosorbent assay (ELISA) method and kit for detecting neomycin, amikacin and paromomycin
InactiveCN102585006ASimple processing methodNo health hazardMicroorganism based processesTissue cultureElisa methodImmuno detection
The invention discloses a specific monoclonal antibody capable of identifying neomycin, amikacin and paromomycin and an enzyme-linked immunosorbent assay (ELISA) method and kit for detecting neomycin, amikacin and paromomycin. The monoclonal antibody is secreted by a hybridoma cell EDC / 5G04 and the hybridoma cell is collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:C201144. The ELISA method comprises the steps of preparation of immunogen, coating antigen and the antibody, treatment and detection of samples and the like. Compared with the prior art, the monoclonal antibody, the ELISA method and the kit have the following main advantages that the prepared monoclonal antibody can simultaneously identify neomycin, amikacin and paromomycin, thus improving the detection efficiency of the prior art; the animal tissue sample treatment method is simple and short in time; and the ELISA method and the kit also have the characteristics of high detection sensitivity, good precision and good accuracy.
Owner:HUAZHONG AGRI UNIV
Compound microbial fertilizer for preventing and treating take-all disease of wheat and preparation method of compound microbial fertilizer
InactiveCN106244490APromote absorptionImprove the effect of disease preventionBiocideFungiDiseaseNutrition
The invention discloses a compound microbial fertilizer for preventing and treating take-all disease of wheat and a preparation method of the compound microbial fertilizer. The compound microbial fertilizer consists of the following raw materials: streptomyces catenulae, streptomyces albolongus, bacillus flexus, bacillus aryabhattai and trichoderma brevicompactum. The strains of the invention, on the basis of the comprehensive utilization of microbial inter-species or intra-species antibiosis, competition, hyperparasitism and bacteriolysis effects or by inducing the wheat to generate disease resistance by virtue of secondary metabolites, can generate various antibiotics, such as proceomycin, neomycin E, catenulin, diparomomycin and the like, so as to inhibit pathogenic bacteria of the take-all disease of wheat; the five strains adopted by the invention are directly separated from soil and have a rhizosphere growth promoting effect; the strains can promote reproduction and growth on crop surfaces or in plants or soil, and meanwhile, the strains can take an effect on nitrogen fixation and can promote the directional secretion of some secondary metabolites from plant rhizosphere; and the strains can enhance nutrition absorption of plants and stimulate plant growth, so that the effects of the fertilizer are achieved.
Owner:FOSHAN YANHUI BIOTECH CO LTD
Methods and Agents for Enhancing Wound Healing
InactiveUS20130237485A1Harmful effectEasy to manageBiocidePeptide/protein ingredientsWound healingGentamicin
This invention discloses a method of inducing expression of full-length collagen 7 in cells that contain nonsense mutations in the COL7A1 gene by treating the cell with an aminoglycoside such as G418, gentamicin, and paromomycin. Also provided is a method of treating DEB due to nonsense mutation in the COL7A1 gene by administering a composition containing an effective amount of an aminoglycoside. Also provided is a novel composition useful to treating conditions due to nonsense mutation in the COL7A1 gene, containing an aminoglycoside, a C7, a min-C7 or both; and a pharmaceutically acceptable carrier.
Owner:UNIV OF SOUTHERN CALIFORNIA
Tetrahymena cell line containing luciferase gene, construction method and applications thereof
InactiveCN102925464ARapid responseEasy to detectProtozoaMicrobiological testing/measurementBiologyParomomycin
The invention relates to a tetrahymena cell line containing luciferase gene, a construction method and applications thereof. According to the present invention, a PCR technology is adopted to optimize a N-terminal sequence and a C-terminal sequence of firefly luciferase gene into tetrahymena preference codons, the optimized firefly luciferase gene LUC is linked to a carrier pBX to obtain firefly luciferase gene-containing recombinant plasmid pBX-LUC, a gene gun particle bombardment method is adopted to transform the firefly luciferase gene-containing recombinant plasmid pBX-LUC into tetrahymena thermophila B2086 cells, intracellular homologous recombination and paromomycin resistance screening are performed, and MTT1 gene in the cells are replaced by the LUC gene to obtain the engineered cell line B2086-LUC, wherein the engineered cell line B2086-LUC expresses luciferase under specific heavy metal induction, coloration is performed after a fluorescent substrate D-Luciferin is added, and the enlarged fluorescence intensity is detected on so as to reflect conditions of heavy metal pollutants in a reaction environment through quantitative data, such that the detection method is rapid and sensitive, and easy to operate. The tetrahymena cell line containing luciferase gene has a wide application prospect in biological monitoring of water body environment heavy metal pollution.
Owner:SHANXI UNIV
Method for improving output of tetraene macrolide antibiotics
InactiveCN104531798AStrong resistanceYield levels increaseMicroorganism based processesFermentationMicroorganismMacrolide resistance
The invention discloses a method for improving output of tetraene macrolide antibiotics and belongs to the technical field of microorganisms. According to the method, 100mu g / mL paromomycin is added in a solid medium which is used for culturing Streptomyces diastatochromogenes D to obtain a Streptomyces diastatochromogenes mutant strain which has relatively good resistance to the paromomycin; the capacity of synthesizing the tetraene macrolide antibiotics of the mutant strain is stronger than that of the Streptomyces diastatochromogenes D.
Owner:CHINA JILIANG UNIV
Colloidal gold test strip for detecting neomycin residual and use method and application thereof
Owner:HUAZHONG AGRI UNIV
Aminoglycosides and uses thereof in the treatment of genetic disorders
Owner:TECHNION RES & DEV FOUND LTD
Application of chlamydomonas reinhardtii LRR (Leucine Rich Repeat) gene in regulating and controlling cadmium resistance of chlamydomonas reinhardtii
InactiveCN107760693ASimple methodImprove efficiencyUnicellular algaeMicroorganism based processesBiotechnologyChlamydomonas reinhardtii
The invention discloses application of a chlamydomonas reinhardtii LRR (Leucine Rich Repeat) gene in regulating and controlling cadmium resistance of chlamydomonas reinhardtii, and belongs to the technical field of biologics and the field of environmental pollution improvement. According to the application, an aphVIII fragment with a paromomycin resistance gene is randomly inserted into chlamydomonas, then a chlamydomonas reinhardtii mutant alga strain which is remarkable in cadmium chloride resistance is cultured, and further identification shows that the cadmium sensitivity of the mutant alga strain is caused since the aphVIII (SEQ ID NO:3) fragment is inserted into an LRR gene (a CDS (Cadmium Sulfide) sequence is shown in SEQ ID NO:1 in the specification). By reducing expression of thechlamydomonas reinhardtii LRR gene, deactivating the LRR gene or by deleting the LRR gene, the cadmium resistance of the chlamydomonas reinhardtii can be improved; a cadmium resistance improved chlamydomonas reinhardtii strain obtained by means of genetic engineering can be applied to monitoring and improvement of cadmium polluted water areas, cadmium polluted water environments can be treated with the cadmium resistance improved chlamydomonas reinhardtii strain, the operation is simple and feasible, and the cost is low.
Owner:JIANGHAN UNIVERSITY
Method for preparing crude paromomycin with membrane separation technology
InactiveCN102391329AEasy to remove impuritiesEasy to separateSugar derivativesSugar derivatives preparationBiotechnologySeparation technology
The invention provides a method for preparing crude paromomycin with a membrane separation technology. The method comprises the following steps: 1, a material liquid containing paromomycin is processed by a microfiltration membrane, and remove the fermentation liquor thallus and the residue; 2, macromolecular impurities of proteins, polysaccharides and coloring substances in the microfiltered permeation liquid obtained in step 1 are removed; 3, the collected ultrafiltration permeation liquid is desalinated by a nanofiltration membrane at normal temperature and is enriched; and 4, the desalinated liquid is concentrated and dried to prepare the crude paromomycin. The method of the invention, which has the advantages of novel conception, mild production technology condition, environment protection, cleaning, simplicity, easy implement, short production period, low production cost, high extraction yield, and strong practicality, has a large popularization and application value.
Owner:福建和泉生物科技有限公司
Method for preparing paromomycin crude product by membrane separation technology
InactiveCN102399248AEasy to remove impuritiesEasy to separateSugar derivativesSugar derivatives preparationMicrofiltration membraneSeparation technology
The invention provides a method for preparing a paromomycin crude product by a membrane separation technology. The method comprises the steps of removing impurities from feed liquid in the preparation process of the paromomycin crude product through a microfiltration membrane, decoloring through ultrafiltration, desalting through nanofiltration, enriching, concentrating and drying and particularly comprises the following steps of: performing liquid solid separation on the feed liquid containing the paromomycin through the microfiltration membrane; removing macromolecular impurities such as protein, pigment, polysaccharide and the like from microfiltration permeate through an ultrafiltration membrane; desalting and enriching the collected permeate through a nanofiltration membrane at normal temperature; and concentrating and drying the desalted liquid to obtain the paromomycin crude product. The method is novel in idea, mild in production process condition, green and clean, simple and practicable, short in production period, low in production cost, high in extraction yield and high in practicability, and has large popularization and application value.
Owner:福建和泉生物科技有限公司
Method for decolorizing and purifying paromomycin
ActiveCN102532214AReduce production stepsHigh yieldSugar derivativesSugar derivatives preparationIon exchangeIon-exchange resin
The invention relates to a method for decolorizing and purifying paromomycin. The method comprises the following steps of: 1) sequentially dividing a plurality of anion exchange resin columns of an anticlockwise rotating continuous moving bed into an aqueous ammonia resolving area, a product separation area, an impurity separation area and a resin regeneration area in a clockwise direction; 2) delivering the liquid discharged from the aqueous ammonia resolving area to a first material tank, delivering the liquid in the first material tank to the product separation area from the inlet of the product separation area, and making the liquid flow to a second material tank from the outlet of the product separation area; 3) supplying a paromomycin mixed solution to be treated to the second material tank, delivering the mixed solution in the second material tank to the impurity separation area from the inlet of the impurity separation area, and making the mixed solution flow out of the outlet of the impurity separation area; and 4) collecting the liquid in the first material tank, and thus obtaining a paromomycin product. A continuous ion exchange technology is used for simultaneous decolorization and impurity removal of the paromomycin mixed solution in the method, so that the production steps are reduced and the yield of the product is improved.
Owner:SANDA FILM SCI & TECH XIAMEN
Hybridoma cell line C1 capable of secreting anti-paromomycin monoclonal antibody and application of hybridoma cell line C1
The invention provides a hybridoma cell line C1 capable of secreting an anti-paromomycin monoclonal antibody and an application of the hybridoma cell line C1 and belongs to the technical field of immunochemistry. A mouse monoclonal cell line C1 is prepared with a conventional cell fusion technology, and is preserved in the CGMCC (China General Microbiological Culture Collection Center) with the preservation number being CGMCC No. 12025. The monoclonal antibody secreted by the cell line is determined by indirect competitive enzyme-linked immunosorbent assay, and the IC50 (half maximal inhibitory concentration) for paromomycin and the IC50 for neomycin are 0.61 mu g / L and 2.43 mu g / L respectively. The recovery rate range for paromomycin and neomycin in animal food is 64.56%, 105.85% and 54.08%-100.55%. Raw materials are provided for immunoassay of paromomycin residues in animal food, and the monoclonal antibody has practical application value.
Owner:DELISI GROUP +1
Application of paromomycin to preparation of medicine for treating trichomonas vaginitis
InactiveCN105726552AGood killing effectAddressing drug resistanceOrganic active ingredientsAntiparasitic agentsSide effectDrug resistance
The invention provides novel application of paromomycin to preparation of medicine for treating trichomonas vaginitis.The application of paromomycin to preparation of medicine for treating trichomonas vaginitis is implemented by inhibiting trichomonas vaginalis.Compared with the prior art, the application of paromomycin to preparation of medicine for treating trichomonas vaginitis has the advantages that paromomycin of low concentration has a good killing action on trichomonas vaginitis, has an obvious effect on inhibition of trichomonas vaginitis and can be used for preparing medicine for treating trichomonas vaginitis so as to solve the problems of drug resistance and high side effect existing in an existing drug.
Owner:杨诚 +2
Tetrahymena expression vector of chitinases and its application in expressing chitinases
ActiveCN102517281ASimple vegetative growthShort life cycleFungiBacteriaHigh level expressionNucleotide
The invention discloses a Tetrahymena expression vector of chitinases and its application in expressing chitinases. The invention provides an expression cassette, which includes an HSP70-2gene promoter as shown in the 9th-1126th nucleotides from the 5' terminal of sequence 1, an encoding gene of chitinases in sequence 2 and an HSP70-1 gene termination signal as shown in the 3835th-4312th nucleotides from the 5' terminal of sequence 1. The invention also provides a recombinant plasmid containing the expression cassette. When transferred to a Tetrahymena through electroporation, the recombinantplasmid can, under the drug screening effect of paromomycin, replace most of the original rDNA in the Tetrahymena, and multiply an exogenous gene in the Tetrahymena massively, thus realizing genetic transformation of a target gene. Under heat shock conditions, the HSP70-2 strong promoter can start the high level expression of CBD3 gene, and the expressed chitinases have bioactivity (able to hydrolyze chitin). In conclusion, the expression vector of the invention has wide application prospects.
Owner:FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES +1
Application of chlamydomonas reinhardtii RFC1 gene in regulation of cadmium tolerance of chlamydomonas reinhardtii
InactiveCN107828801ASimple methodMature technology systemUnicellular algaeWater contaminantsChlamydomonas reinhardtiiAgricultural science
The invention discloses application of a chlamydomonas reinhardtii RFC1 gene in the regulation of cadmium tolerance of chlamydomonas reinhardtii and belongs to the fields of biotechnology and environment pollution control. A chlamydomonas reinhardtii mutant strain capable of resisting cadmium chloride obviously is acquired by randomly inserting an aphVIII segment (SEQ ID NO: 3) containing a paromomycin resistance gene into chlamydomonas, and further identification discovers that the cadmium resistance of the mutant strain is caused by inserting the aphVIII segment into the RFC1 gene (the CDS sequence is as shown in SEQ ID NO: 1). By reducing the expression of the chlamydomonas reinhardtii RFC1 gene, the RFC1 gene is deactivated or deleted so as to improve the cadmium tolerance of the chlamydomonas reinhardtii; the chlamydomonas reinhardtii strain with enhanced cadmium tolerance and acquired through a gene engineering method can be applied to the monitoring and treatment of a cadmium-polluted water area and provides a new direction for sewage treatment.
Owner:JIANGHAN UNIVERSITY
Method for increasing yield of toyocamycin through screening of united drug-resistant mutant strains
InactiveCN105349476ARaise production levelsIncreased ability to produce toyocamycinBacteriaMicroorganism based processesHigh concentrationMicroorganism
The invention discloses a method for increasing yield of toyocamycin through screening of united drug-resistant mutant strains and belongs to the field of microbial technologies. According to the method, Streptomyces diastatochromogenes D mutant strains generating drug resistance to low-concentration streptomycin, high-concentration streptomycin and paromomycin are obtained through screening of the united drug-resistant mutant strains, and the toyocamycin productivity of the obtained mutant strains is remarkably improved when compared with that of Streptomyces diastatochromogenes D.
Owner:CHINA JILIANG UNIV
Application of Chlamydomonas reinhardtii g3280.t2 gene for regulating and controlling cadmium tolerance of Chlamydomonas reinhardtii
InactiveCN108004252ASimple methodImprove efficiencyMicrobiological testing/measurementUnicellular algaeChlamydomonas reinhardtiiSewage
The invention discloses application of Chlamydomonas reinhardtii g3280.t2 gene for regulating and controlling cadmium tolerance of Chlamydomonas reinhardtii, and belongs to the fields of biotechnologyand environmental pollution control. A Chlamydomonas reinhardtii mutation strain obviously sensitive to cadmium is obtained by inserting the aphVIII fragment (SEQ ID NO:3) containing a paromomycin resistance gene into chlamydomonas at random, and further identification discovers that the cadmium sensitivity of the mutation strain is caused by the fact that the aphVIII fragment is inserted into the g3280.t2 gene (CDS sequence is shown as SEQ ID NO: 1). By reducing the expression of the Chlamydomonas reinhardtii g3280.t2 gene, the g3280.t2 gene is inactivated or the cadmium tolerance of Chlamydomonas reinhardtii can be reduced by losing the gene; and the cadmium sensitive Chlamydomonas reinhardtiis train obtained through gene engineering means can be applied into water environments for monitoring and detection of cadmium pollution through biological means, and then a novel biological material is provided for sewage detection.
Owner:JIANGHAN UNIVERSITY
Application of chlamydomonas reinhardtii VMPL1 gene to regulation of cadmium tolerance of chlamydomonas reinhardtii
InactiveCN107904248ASimple methodMature technology systemMicrobiological testing/measurementUnicellular algaeChlamydomonas reinhardtiiAgricultural science
The invention discloses application of chlamydomonas reinhardtii VMPL1 gene to the regulation of the cadmium tolerance of chlamydomonas reinhardtii, and belongs to the fields of biotechnology and environmental pollution control. According to the invention, an aph VIII segment (SEQ ID NO:3) containing paromomycin resistance gene is randomly inserted into chlamydomonas, chlamydomonas reinhardtii mutant strain obviously sensitive to cadmium is obtained, and through further identification, the fact that the cadmium resistance of the mutant strain is caused because the aph VIII segment is insertedinto the VMPL1 gene (CDS sequence is as shown in SEQ ID NO:1) is discovered. By reducing the expression of the chlamydomonas reinhardtii VMPL1 gene to enable the VMPL1 gene to be inactivated or deleted, the VMPL1 gene can improve the cadmium tolerance of chlamydomonas reinhardtii; the chlamydomonas reinhardtii strain with enhanced cadmium tolerance obtained through a gene engineering method can beapplied to the mentoring and control of cadmium polluted waters. According to the invention, a novel thought and a biological material are provided for sewage treatment.
Owner:JIANGHAN UNIVERSITY
Application of Chlamydomonas reinhardtii Cre01.g046237.t1.1 gene in regulating cadmium tolerance of Chlamydomonas reinhardtii
InactiveCN107828799ASimple methodMature technology systemUnicellular algaeWater contaminantsChlamydomonas reinhardtiiMicrobiology
The invention discloses an application of Chlamydomonas reinhardtii Cre01.g046237.t1.1 gene in regulating cadmium tolerance of Chlamydomonas reinhardtii. Through inserting aph VIII segment containinga paromomycin resistance gene in Chlamydomonas at random, the Chlamydomonas reinhardtii mutated strain capable of resisting cadmium chloride obviously is acquired, the further identification finds that the cadmium resistance of the strain is caused by inserting the aph VIII segment (SEQ ID NO: 3) in Cre01.g046237.t1.1 gene. Through reducing the expression of the Chlamydomonas reinhardtii Cre01.g046237.t1.1 gene (the CDS sequence is shown as SEQ ID NO: 1), the deactivated or deficient gene can improve the cadmium tolerance of Chlamydomonas reinhardtii; the Chlamydomonas reinhardtii strain withenhanced cadmium tolerance acquired through gene engineering method can be applied to the monitoring and governing of a cadmium polluted water area, and provides a new direction for sewage governing.
Owner:JIANGHAN UNIVERSITY
Treatments for free-living amoebic infections
ActiveUS10806741B2Effective treatmentReduce adverse side effectsPharmaceutical delivery mechanismPhosphorous compound active ingredientsAmoebic encephalitisSappinia
Methods of treating infections caused by free-living amoeba are disclosed. The methods generally include systemic administration of an effective amount of miltefosine, such as an oral or intravenous formulation, and optionally local administration of an effective amount of miltefosine, such as a topical formulation of miltefosine. The methods may further include administration of one or more secondary agents. Exemplary secondary agents include steroids, polyhexamethylene biguanide (PHMB), chlorhexidine, propamidine isethionate, dibromopropamidine isethionate, neomycin, paromomycin, polymyxin B, clotrimazole, ketoconazole, miconazole, and itraconazole. The methods may be used to treat patients with infections caused by a free-living amoeba such as Naegleria fowleri, Balamuthia mandrillaris, Sappinia diploidea, and Acanthamoeba species. Exemplary infections include Acanthamoeba keratitis, granulomatous amoebic encephalitis, cutaneous acanthamoebiasis, primary amoebic meningoencephalitis, Sappinia amoebic encephalitis, or a disseminated disease associated with a free-living amoeba.
Owner:PROFOUNDA
Method for decolorizing and purifying paromomycin
ActiveCN102532214BReduce production stepsHigh yieldSugar derivativesSugar derivatives preparationIon exchangeAmmonia
Owner:SANDA FILM SCI & TECH XIAMEN
Paromomycin supplemented feedingstuffs for poultry species and use thereof for prophylaxis of histomoniasis, reduction of the horizontal spreading of histomoniasis, and for improved weight gain and feed conversion
ActiveUS20100137242A1Improvement of zoo-technical performanceWeight increaseBiocideOrganic active ingredientsDiseaseMortality rate
Disclosed is the continuous supplementation of poultry feeding stuffs with paromomycin and the resulting effects of prophylaxis against histomoniasis, ensuing decrease of mortality and the reduction of horizontal spreading of the disease. Also observed are improved zoo-technical performances, increase in weight gain and feed efficiency, both in healthy as well as diseased birds.
Owner:HUVEPHARMA EOOD
Tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof
InactiveCN101586119BImprove relevanceIncrease credibilityMicrobiological testing/measurementChemiluminescene/bioluminescenceGene vectorBiology
The invention discloses a tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof. Plasmid T-GFP is constructed by using plasmid rDNA-GFP in the methodssuch as enzyme cutting and PCR enzyme cutting site mutation; thermophile tetrahymena genome DNA is used for constructing plasmid T-HSP702 in the methods such as PCR augmentation and clone; Plasmids T-GFP, T-HSP702 and pD5H8 are used for construction and finally obtaining plasmid HSP702-GFP-pD5H8 by enzyme cutting and connection. The carrier contains HSP702 promoter, thereby being capable of realizing high-efficient expression of exogenous gene; the carrier contains replaceable exogenous gene gfp so that an exogenous gene expression system is realized for tetrahymena by replacing different exogenous genes; with the sieving effect of paromomycin medicine, the carrier replaces most rDNAs originally in the tetrahymena so that the exogenous genes are largely increased in the tetrahymena so asto realize genetic transformation of target genes. The method in the invention is easy and has convenient operation, and the carrier can be used for detection of environmental tributyl tin in transfection in tetrahymena.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Application of chlamydomonas reinhardtii VPS9 gene in regulating and controlling cadmium resistance of chlamydomonas reinhardtii
InactiveCN107760694ASimple methodMature technology systemUnicellular algaeMicroorganism based processesBiotechnologyChlamydomonas reinhardtii
The invention discloses application of a chlamydomonas reinhardtii VPS9 gene in regulating and controlling cadmium resistance of chlamydomonas reinhardtii, and belongs to the technical field of biologics and the field of sewage improvement. According to the application, an aphVIII fragment (SEQ ID NO:3) with a paromomycin resistance gene is randomly inserted into chlamydomonas, then a chlamydomonas reinhardtii mutant alga strain which is remarkably sensitive to cadmium is cultured, and further identification shows that the cadmium sensitivity of the mutant alga strain is caused since the aphVIII fragment is inserted into a VPS9 gene (a CDS (Cadmium Sulfide) sequence is shown in SEQ ID NO:1 in the specification). By reducing expression of the chlamydomonas reinhardtii VPS9 gene, deactivating the VPS9 gene or by deleting the VPS9 gene, the cadmium resistance of the chlamydomonas reinhardtii can be degraded; a cadmium-sensitive chlamydomonas reinhardtii strain obtained by means of geneticengineering can be applied to monitoring and detection on cadmium polluted water areas, and novel ideas and biological materials can be provided for sewage treatment by using a biological method.
Owner:JIANGHAN UNIVERSITY
A hybridoma cell line c1 secreting anti-paromomycin monoclonal antibody and its application
Disclosed are a hybridoma cell strain C1 for secreting an anti-paromomycin monoclonal antibody and the use thereof, belonging to the technical field of immunochemistry. A murine monoclonal cell strain C1 has been prepared through the conventional cell fusion technologies and deposited in the China General Microbiological Culture Collection Centre, and the deposit number is CGMCC No. 12025. The monoclonal antibody secreted by the cell strain is determined by the indirect competitive enzyme-linked immunosorbent assay, and the IC50 to paromomycin and neomycin is 0.61 and 2.43 μg / L respectively. The range of the recovery rate of paromomycin and neomycin in animal foodstuff is 64.56-105.85% and 54.08-100.55%. The hybridoma cell strain C1 provides a raw material for the immunological detection of the paromomycin residue in animal foodstuff, and has a practical application value.
Owner:DELISI GROUP +1
Veterinary composition for preventing and/or treating cryptosporidiosis
PendingCN114258302AEasy dischargeReduce and/or eliminate dischargeOrganic active ingredientsDispersion deliveryZoologyPharmacology
The present invention relates to a veterinary composition comprising paromomycin or a pharmaceutically acceptable salt thereof for use in the prevention and / or treatment of cryptosporidiosis in a non-human mammal wherein the composition is administered to the non-human mammal at a paromomycin dosage of 80 to 140 mg / kg / day for 3 to 6 days.
Owner:法国诗华大药厂
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