202 results about "Pig reproduction" patented technology

Pigs challenged with hypoglycosylated variants of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) major surface protein GP5 exhibited increased production of PRRSV-neutralizing antibodies relative to the levels of neurtalizing antibodies produced by pigs immunized with wild type (wt) or glycosylated GP5. This invention provides for methods of obtaining improved immune responses in pigs to PRRSV, compositions useful for obtaining the improved immune responses as well as isolated polynucleotides that encode hypoglycosylated variants of PRRSV major surface protein GP5.

The invention relates to feed, in particular to green-grape livestock and poultry feed suitable for pigs, chicken and so on, which is prepared from grape pip powder or grape pip meal or ripening grape pin powder, corn, soybean meal, DDGS, corn fiber feed, bone powder, calcium monohydrogen phosphate, limestone, and salt according to certain ratio. The feed has the characteristics of saving corn, improving the performance of the livestock and poultry, and improving the economic benefit. The feed experiment indicates that 4 to 10 percent of grape pin is added to pigwash for a female pig without harmful effect on the female pig reproduction, the weight for baby pigs which are ablactated is increased, the weight of fatting pigs is increased, and furs for the fed pigs are bright, the egg-lay performance of laying hen can be improved, and the crude fiber content of the feed can be adjusted.

The invention discloses a traditional Chinese medicine for treating respiratory diseases and promoting growth for swine and a preparation method thereof. The traditional Chinese medicine is composed of plantain herb, herba patriniae, hindu datura flower, perilla leaf, trichosanthes kirilowii maxim and gelsmium elegans. The traditional Chinese medicine of the invention can effectively prevent and treat livestock respiratory diseases, such as swine enzootic pneumonia, infectious pleuropneumonia of swine, porcine reproductive and respiratory syndrome (PRRS), swine plague, swine influenza and thelike; meanwhile, the traditional Chinese medicine of the invention can improve animal productivity and promote animal growth. The traditional Chinese medicine of the invention has wide raw material resources, is safe and environmentally-friendly and has the advantages of simple preparation technology, low production cost and the like.

The invention relates to a feed, a preparation method and use thereof in improvement in the reproductive performance of sows. The feed is characterized by comprising the following components in percentage by weight: 0.05 to 0.2 percent of N-carbamylglutamic acid and the balance of auxiliary materials. The preparation method comprises the following steps of: 1) removing impurities from the auxiliary materials in percentage by weight, air-drying the auxiliary materials and crushing the auxiliary materials to 20 to 100 meshes; and 2) mixing under stirring the crushed auxiliary materials obtainedin the step 1) and the N-carbamylglutamic acid in percentage by weight, and sieving the mixture to obtain the finished product feed. The feed can enhance the reproductive performance of the sows, canpromote the synthesis of endogenous arginine in the sows and effectively improve the reproductive performance of the sows to further improve the growth performance of piglets.

The present invention relates to pig reproduction and respiratory syndrome virus recombined adenovirus and vaccine, and belongs to the field of biological high-tech. Through RT-PCR process to proliferate whole PRRSV GP5 sequence, cloning the gene sequence to the shuttle vector pShuttle-CMV of adenovirus carrier system, cotransforming colibacillus BJ5183 strain together with the skeleton vector of adenovirus carrier system to obtain recombinant plasmid, transfecting HEK293-A cell to obtain recombinant adenovirus and plaque purification, and RT-PCR and indirect immunofluorescence technique inspection, the recombinant adenovirus rAd-GP5 expressing PRRSV GP5 protein is constituted. The recombinant adenovirus can set ahead the expression of PRRSV GP5 protein and raise the expression amount to simulate the immune protecting reaction of body effectively.

The invention is an ELISA reagent box to detect pig progenitive and respiratory syndrome (PRRS) antibody as well as detecting method. The reagent box including: ELISA slat with reformed N protein as coating antigen, PBS solution, blood serum diluted solution, rabbit anti-pig enzyme-labeled bi-antibody, color-developing substrate, terminating liquor, PRRS positive blood serum, PRRS negative blood serum, etc. After tested by the indexes of peculiarity, sensitivity, repetition and so on, the detecting method is used to detect the blood serum sample. Its advantages: it has good peculiarity, able to be largely produced and has low detecting cost. The detecting coincidence with ELISA reagent box of IDEXX Company is 91%, largely reducing the detecting cost.

The invention discloses a prescription composition of a pig antiviral semen diluent. Based on every 1000ml of double distilled water, the pig antiviral semen diluent contains 15.50g of glucose, 11.65g of trisodium citrate, 2.35g of EDTA sodium salt, 1.75g of sodium bicarbonate, 1.00g of polyvinyl alcohol, 5.50g of tricarboxymethyl amino methane, 4.10g of citric acid, 0.07g of cysteine, 1.00g of vitamin C, 0.10g of enrofloxacin and 50ml of an antiviral agent. According to the embodiment of the invention, the diluent of the prescription composition of the pig antiviral semen diluent has obvious killing, inhibition and obstructing effects on pig reproduction and respiration syndrome virus (PRRSV) and is capable of obviously improving the survival rate and preservation time of semen during in vitro preservation and the litter size of a mated sow.

The invention belongs to the animal gene engineering technology field. It is about a gene and its application, the gene is modificatory porcine reproductive and respiratory syndrome virus ORF5. Its attribute is to insert nucleotide sequence between the neutralization and cover list of GP5, the nucleotide sequence is artificial synthesis coding accessorial T-lymph cell list. The sequence of this nucleotide is just as the sequence list of SEQ ID NO:1 and attached figure 1. This modificatory gene is included in eukaryotic expression plasmid, and the Escherichia coliDH5 / pCI-52M, which includes the plasmid, is conserved in CCTCC, and the number is CCTCC NO: M204080. This invention also presents the use of this gene in the preparation of pig bread and respiratory syndrome DNA vaccineíú

The invention discloses a yeast complex additive with high-efficiency food attraction and immunological enhancement functions. The additive comprises the following active ingredients yeast nucleic acid, yeast glucan, yeast mannan, and selenium-enriched yeast. The additive is mainly applied in powder or particulate feeds of pigs, the addition amount is only 0.1-0.3%, and the additive has important application values in improving the growth performance of weaned piglets, enhancing the organism immunity capability of piglets, increasing the reproductive performance or anti-oxidization capability of sows, and increasing the contents of colostrum component and immunoglobulin of sows.

The invention provides a medicine which can be used to effectively treat swine high fever. The drug efficacy components are produced with the raw materials in the following parts by weight: gypsum is 100 to 200 parts, scutellaria is 80 to 150 parts, dried rehmannia root is 80 to 150 parts, moutan bark is 80 to 150 parts, atractylodes rhizome is 80 to 150 parts, phellodendron bark is 80 to 150 parts, cape jasmine is 80 to 150 parts, and rhubarb is 80 to 150 parts. Together with pharmaceutically acceptable carrier, the medicine can be produced into various dosage forms, such as powder, mixture, tablets, capsules as well as granules, etc. The medicine is suitable for symptoms of damp heat accumulation, high fever, spirit depression, inappetence, cough and dyspnea, disturbance in respiration, even pneumonia, and constipation which are caused by gradual intrusion of toxic heat into nutrient and blood, and induced weak fetus, even dead fetus and mummy fetus, etc. when the toxic heat attacks pregnant pigs. The medicine has good curative effect for weanling pigs, fattening pigs and brood sows, with up to 85 percent total effectiveness rate.

The invention provides a breeding sow feed additive. The additive is a granular preparation prepared by mixing corn protein powder, fish bone meal, table salt, compound vitamin, fish oil, folic acid, calcium hydrogen phosphate, lysine, methionine, ferrous sulfate, L-lactic acid and fumaric acid. According to the breeding sow feed additive, not only can the sufficient nutrition be supplied to the growth of breeding sows, but also the immunity of the breeding sows can also be enhanced; the absorption to the feed is accelerated and the breeding performance of the breeding sows is improved effectively; the number of living piglets is increased and the economic benefits are increased.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A / G and G / A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention relates to a functional glycoconjugate (chitosan oligosaccharide, fructo oligosaccharide and yeast polysaccharide), and is a functional glycoconjugate for improving sow breeding performance. The functional glycoconjugate can be used as an effective ingredient of a sow feed additive or a premixed feed, belongs to the technical field of agricultural biological products, and comprises the chitosan oligosaccharide, fructo oligosaccharide and yeast polysaccharide. Each ton of feed is added with 20-100g of the chitosan oligosaccharide, 200-1000g of the fructo oligosaccharide and 500-2000 g of the yeast polysaccharide. The components of the functional glycoconjugate are pure biological preparations, free of toxic and side effects, free of residues, less in addition amount, and good in taste palatability. The functional glycoconjugate can alleviate the sow constipation, reduce the damage caused by stress reaction, enhance immunity, and improve the overall health level of sows, and can increase the sow farrowing number, reduce the number of weak piglets and the number of stillbirth, prolong the using life of the sows and significantly improve the number of weaned piglets and the litter weight of the weaned piglets.

The invention discloses application of a microRNA (micro ribonucleic acid)-miR-23a molecular marker miR-23a to quickly detecting the quality of oocytes of sows. The miRNA molecular marker miR-23a in the cumulus oocytes with low potentiality development is abnormally high in expression and can be secreted in serum via active secretion procedures of tissues, and serum miR-23a is the molecular marker with a function of quickly detecting the quality of the oocytes of the sows as discovered by large-sample detection on the expression level of the serum miR-23a of the sows with low production performance. The application has the advantages that the reproductive potential of the sows can be predicted by means of detecting the quality of the oocytes, so that the sows with low production potential can be eliminated as early as possible, the feed and labor costs can be greatly reduced, and the application is beneficial to vigorous development of the pig industry in China.

The invention provides feed for a sow. The feed is prepared from corn flour, peanut meal, wheat middling, corn gluten meal, fish bone meal, salt and premix, wherein the premix comprises a pure organic acid, vitamins and a variety of micro-minerals. The feed not only can supply adequate nutrition for growth of the sow, but also can enhance the immunity of the sow, accelerate the feed absorption of the sow, improve the reproductive performance, increase the number of born alive piglets and enhance the economic benefit.

The invention provides a p porcine pseudorabies virus and vaccine composition and applications thereof, belonging to the field of biotechnology. The microbial preservation number of the porcine pseudorabies virus PRV-JS strain is CGMCCNO.6604. The invention further provides a vaccine composition which comprises an inactivated porcine pseudorabies virus PRV-JS strain and adjuvant acceptable on veteriary pharmacy. The vaccine composition further comprises a carrier acceptable on the veterinary pharmacy. The porcine pseudorabies virus PRV-JS strain is screened from porcine pseudorabies prevalent strains separated from all pig farms and has good immunogenicity, and can be used as inactivated vaccine production virus seeds or virus seeds for testing. After being immunized by the vaccine composition, pigs have higher produced antibody level and the lasting period is long. The vaccine composition prepared by adopting the porcine pseudorabies virus PRV-JS strain can be used for preventing the sow abortionbreeding difficulty and mortality syndromeboar infertility caused by the porcine pseudorabies virus, boar infertility and pseudorabies of other pigs.

The invention provides a method for improving a sow pregnancy rate. The method comprises the steps of pre-breeding preparing, seminal fluid collecting and storing before breeding is carried out and estrus detection and breeding of a breeding sow. According to the method, the pregnancy rate of a sow can be increased more than 95%, the empty pregnancy rate for sow breeding is greatly lowered, the service life period of the sow is prolonged, the morbidity of a sow breeding system is greatly lowered, and the piglet birth rate is stable.

The invention relates to detection reagents and detection methods for porcine reproductive and respiratory syndrome virus (PRRSV). On the one hand, the invention provides immunogenic fragments in coded non-structural protein 2 (Nsp2), and in particular, the immunogenic fragments provided in the invention exist in Nsp2 protein of wild type PRRSV instead of in Nsp2 protein of attenuation PRRSV; on the other hand, the invention also provides oligonucleotide primers, and in particular, a to-be-amplified area of the oligonucleotide primers provided in the invention includes at least a part of the coding sequence of Nsp2 protein and the part of the coding sequence exists in Nsp2 coding sequence of wild type PRRSV instead of in Nsp2 coding sequence of attenuation PRRSV. The invention further provides the methods of using relevant detection reagents in detection and a method of using the above-mentioned methods to distinguish pigs immunized by attenuation PRRSV from pigs infected by wild type PRRSV.

The invention discloses an application of an inhibin recombinant fusion protein to preparing medicines for promoting oestrus and hybridization of sows. When inhibin is used for the sows: 0.5-1mg of the inhibin recombinant protein is used for initial immunization; and after 15-21 days, 0.25-0.5mg of the inhibin recombinant protein is used for second immunization. The immune inhibin can increase the estrus rate and the litter sizes of the sows. The invention has obvious effects on overcoming anestrus of the sows, caused by heat stress in summer, promoting the breeding activities of breeding sows, and particularly on promoting oestrus of breeding sows and back-up sows and increasing litter sizes. Thus, the non-productive time of the breeding sows is shortened, the breeding performance of thebreeding sows is improved, and the economic benefit of sow raising is increased.

The invention discloses a sow feeding mode for improving the sow breeding performance and a matched sow feed. According to the feeding mode, after multiparous sows are bred, feed is restricted strictly within 0-5 d, the feeding amount of matched feed at the pregnancy earlier stage is 2-2.2 kg, and at 6-50 d of the gestation period, the feeding amount is increased to 2.5-2.7 kg; within 51-85 d of the sow gestation period, the matched feed of the pregnancy earlier stage is fed, 2.3-2.5 kg of the feed is fed to each sow, a back fat instrument is adopted for monitoring the thickness of sow back fat to be 16-18 mm; the feeding amount is corresponding decreased or increased by 0.2-0.3 kg every time the thickness of the back fat is 1 mm lower than or large than 16-18 mm. Compared with the sow feed matched with the sow feeding mode, the feed includes the pregnancy earlier stage feed, the pregnancy later stage feed and the milking sow feed. The propagation characteristics of the stages of the sows are combined, feeding programs and feed nutrients meeting the sow breeding requirements are applied, and the sow breeding performance and health degree thereof can be effectively improved.

The invention discloses a medicinal composition for increasing pig's reproductive performance, which is prepared from glycerin, olive oil, spinacene and dried wheat flour.

The invention discloses a porcine reproductive and respiratory syndrome virus Nsp2 protein nano-antibody and an application thereof. The nano-antibody is named Nb12, and the amino acid sequence of thenano-antibody is represented by SEQ ID NO.3. A camel is immunized with a truncated expression Nsp2 recombinant protein, camel peripheral blood lymphocytes are separated, and an immunized bactrian camel VHH gene is amplified by using RT-PCR, the amplified gene is connected into a pCANTAB 5E phage vector to construct a bactrian camel heavy chain antibody variable region library, 44 Nsp2 specific nano-antibodies are screened out through three rounds of panning by utilizing a phage display technology, tests prove that the nano-antibodies can specifically react with a Nsp2 protein, and the Nb12 has strong binding force. Antiviral test results show that when the virus inoculation amount is 0.01 MOI, the nano-antibody Nb12 shows a virus replication inhibition effect on both PAM cells and Marc-145 cells. Therefore, a new technical means is provided for subsequent research of the PRRSV Nsp2 and prevention, control and diagnosis of the PRRSV.

The invention discloses application of a pig SNP (Single Nucleotide Polymorphism) molecular marker in screening of reproductive traits and breeding of pigs, wherein the reproductive traits are total litter size traits and live litter size traits; the nucleotide sequence of the molecular marker is as shown in the sequence table SEQ ID NO.1; and T / C base mutation exists at the 70bp position of the sequence. The invention further provides a primer pair used for amplifying the molecular marker and a method for breeding by utilizing the molecular marker. The invention discovers that one SNP molecular marker in the pig OLR1 gene is related to the total litter size traits and the live litter size traits of pigs for the first time; therefore, the SNP molecular marker can be used for marker assisted selection breeding of pigs; namely, the invention provides new use of one SNP molecular marker in the pig OLR1 gene in marker assisted breeding of the reproductive traits of pigs; early screening ofthe reproductive traits of pigs is realized; and a screening method is simple and rapid.

The invention discloses a sow feed used in a gestation period and a breeding method of a sow. The sow feed comprises a feed before gestation, a feed after gestation and a feed for a lactation period. By virtue of the feed and the breeding method, the feed intake of the sow at the lactation period is increased, so that the intake of nutrients by the sow at the lactation period is increased; the reproductive performance of the sow is improved; the vitamin nutrient requirements are met; the health levels of a pregnant sow and embryos are improved; the development of mammary gland of the pregnant sow is improved; the death rate of the embryos is significantly lowered; and the birth weight of piglets is improved.

The invention provides a multi-RT-PCR method for monitoring pollution of six main viruses in a pig farm environment through one system and application. According to the method, primer design is conducted for CSFV, JEV, PRRSV, PCV-2, PRV and PPV, a multi-RT-PCR reaction system for the six viruses is established, air samples are collected through liquid collision, the viruses are subjected to separation concentration through ultrafiltration centrifugation, and the method for detecting the pollution of the main viruses in the pig farm environment is established. The method can be used for monitoring the pollution of the six viruses in air, fodder, drinking water, appliances, excrement and pig groups in the pig house and pig farm environment, and technical support is provided for establishing a main virus pollution early-warning mechanism and a main virus disease preventing, controlling and purifying system in the pig farm environment.

The present invention provides a preparation method for an anti-porcine reproductive and respiratory syndrome cloned pig, comprising: transferring CRISPR / Cas9 targeting vectors and CD163 gene homologous recombination modification vectors into fibroblasts of a pig to obtain positive clone cells, a seventh exon of porcine endogenous CD163 gene being replaced with a eleventh exon of human CD163-L1 gene, so that the positive clone cells are incapable of mediating invasions of PRRSV; taking the positive cell as nuclear transfer donor cells and oocytes as nuclear transfer recipient cells to obtain a cloned embryo by adopting a somatic cell nuclear transfer technology; and impregenating a pig by transferring the cloned embryo into the uterus of the pig, to obtain a cloned pig.

The invention discloses a system for simultaneously detecting six pig viruses and a special LAMP primer for the system. The system comprises an LAMP complete primer for detecting the six pig viruses and / or a reagent required for LAMP and / or an instrument and / or an amplified product data processor, wherein the amplified product data processor is used for distinguishing whether a to-be-detected sample amplification product obtained through loop-mediated isothermal amplification on a to-be-detected sample through the LAMP complete primer for detecting the six pig viruses comprises a specific amplified product or not. The system for detecting the six pig viruses and the special LAMP primer for the system can meet simultaneous and rapid detection of a pig foot-and-mouth disease virus O / A / Asia type I, a hog cholera virus, a porcine respiratory and reproductive syndrome virus American type, a porcine circovirus type II, a porcine parvovirus and a porcine pseudorabies virus, the detection system is simple in operation, the result is convenient to observe and a strong technical support is provided for epidemic disease monitoring of a pig farm.

The present invention relates to pig follicle stimulating hormone beta sub-gene haplotype separating and identifying method, and belongs to the field of livestock molecular biological technology. The method includes: extracting pig's genome DNA, PCR amplification of pig follicle stimulating hormone beta sub-gene, polymorphism detection of the amplified product, constitution of haplotype and pig's breeding character correlativity analysis. The present invention features the separated and identified new pig female hormone receptor gene haplotype H21. The new pig female hormone receptor gene haplotype H21 of the present invention is obviously correlative to pig's breeding character, and the present invention provides new molecular marking method for pig's marking assisted breeding and matching.

The invention provides a replacement gilt mixture capable of increasing breeding rate and a preparation method thereof and relates to the field of feed. The replacement gilt mixture is composed of, by weight, 31-56 parts of corn, 11-26 parts of husked barley, 3-15 parts of bran, 2-8 parts of NO.3 powder, 0.01-0.1 part of flavone, 0.01-0.05 part of curcumin and 0.02-0.07 part of probiotic. The replacement gilt mixture can effectively increase rate of utilizing feed nutritional ingredients by sows, can improve growing performance, nutritional substance digestion rate and intestinal health of the sows and can increase ovulation and farrowing number and improve reproductive performance of the sows.

The invention discloses a method for constructing a model animal, which is used in the research area of veterinary lemology, pathology and pharmacology and relates to non-pig pig breeding, respiratory syndromes and porcine circovirus 2 type diseases. The method is characterized in that in an economical common experimental animal, a typical pathologic change is generated in a mouse through a specific toxic material attacking method, the successful construction of a disease model is proved by adopting the methods for weight weighing, pathological section preparation, PCR detection and ELISA antibody detection, a further basis is provided for an in vivo experimental research correlative with the co-infection of two viruses, and a certain basis is established for a pharmacological experiment research. The disease model in the method is high in stability, simple and convenient in operation and practical in a wide range. Because a pig body experiment has a long period and a high cost, a feminine pig is difficult to screen, and more importantly, a disease cannot be copied in a pig body, the construction of a small animal infection model is very necessary for vaccine immune effect evaluations and virus pathogenic mechanism researches.