83 results about "Salmonella infection" patented technology

The present invention pertains to a Salmonella microorganism having an attenuating mutation which disrupts the expression of a gene located within the Spi2 pathogenicity island, and an auxotrophic mutation. The microorganism therefore has a double mutation which helps prevent reactivity of the microorganism while maintaining the effectiveness of the microorganism to elicit an immune response. The present invention also pertains to vaccine compositions and methods for treating and preventing a Salmonella infection in a patient.

The invention provides lactobacillus plantarum resistant to enteritis salmonella infection and the application thereof and belongs to the technical field of microorganisms.The lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on Sep, 24th, 2015 with the preservation number of CGMCC No.11446.The lactobacillus plantarum CGMCC No.11446 has high acid and cholate resistance, and has a remarkable inhibition effect on growth of enteritis salmonella.Adhesion of enteritis salmonella to enterocyte HT-29 is strongly inhibited, and enteritis salmonella infection is prevented or relieved.The lactobacillus plantarum CGMCC No.11446 can be used for preparing pharmaceutical compositions for preventing or relieving enteritis salmonella, food containing activity lactobacillus and fermented food, and has broad application prospects.

The invention relates to a compound preparation of ceftiofur sodium, in particular to the application of a compound preparation of ceftiofur sodium in preparing medicines for treating colibacillus or salmonella infection of chicken and swine. The compound preparation of ceftiofur sodium comprises the following formula in parts by weight: 0.5-8 parts of ceftiofur sodium, 0.5-6 parts of fosfomycin sodium, 1-3 parts of sulbactam sodium and 4-7 parts of enorfloxacin sodium. The formula can be prepared into a premixed agent, a drink agent or an injection in the using process. The applicant of the invention, by summarizing of many years of tests and according to the antibacterial synergy principle, prepares the compound preparation of ceftiofur sodium which effectively solves the drug tolerance problem of the colibacillus and salmonella, simultaneously improves the action effect and the field of application and improves the survival rate of livestock and fowl.

The invention discloses a salmonella bacteriophage and an application thereof in medicines for preventing and treating salmonella infection diseases. The bacteriophage is named as SPP11 and is preserved in China General Microbiological Culture Collection Center, wherein the preservation date is Nov.04,2019, and the preservation number is CGMCC No.18868. The bacteriophage SPP11 with a strong cracking spectrum has a strong cracking effect on salmonella, can effectively prevent and control salmonella diseases in poultry farms, reduce positivity of the pullorum disease antibody of chickens, reducediarrhea of chickens caused by salmonella enteritis, and can also be used for salmonella sterilization of feeding environment, feed, drinking water and the like. In addition, the bacteriophage is easy for industrial production, and medicines or disinfectants prepared by the bacteriophage not only have reduced cost, but also have the advantages of environmental friendliness.

The invention provides a kit used for detecting swine salmonella antibody. The kit comprises antigen protein; the antigen protein is fljB protein, and the amino acid sequence of the antigen protein isrepresented by SEQ ID NO.3. The fljB protein is excellent in antigenicity, and high in specificity, purity, and yield; in the kit, the antigen protein coating concentration is 20<mu>g / ml, and negative and positive serum dilution ratio is 1:200. The kit can be used for swine salmonella fljB antibody rapid detection of clinical samples, is capable of solving a problem in the prior art that there isno single antigen salmonella infection diagnosis method, reducing failure detection rate, and increasing precision. A sensitive detection method is provided for swine salmonella epidemiological investigation in China, and the kit can be used for early diagnosis of swine salmonella infection.

The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.

The invention provides an application of thymol to preparation of medicines for treating salmonella infection. By using a human cervical carcinoma cell (Hela) as an infection carrier, gentamicin protective experiments, in vitro biochemical experiments and mouse salmonella infection models all verify that thymol has obvious inhibiting effects on salmonella infection by affecting the functions of secretory systems III. Antibiotics easily induce generation of medicine resistance in the process of treating salmonella infection. Compared with the antibiotics, the traditional Chinese medicine compound thymol can directly inhibit the functions of the salmonella secretory systems III and play a role in resisting infection, does not easily induce generation of medicine resistance and has the characteristic of high cure rate.

The invention relates to application of sanguinarine hydrochloride to preparation of medicine for treating salmonella infection. Human cervical carcinoma cells (hela) serve as an infection carrier, and it is verified through gentamicin protection experiments, in-vitro biochemical experiments and mouse salmonella infection models that sanguinarine hydrochloride has a remarkable inhibition effect on salmonella infection. In the process of treating salmonella infection through antibiotic, medicine resistance can be induced to be generated easily, and compared with antibiotic treatment, the function of salmonella type III secretory system can be directly inhibited by the traditional Chinese medicine compound sanguinarine hydrochloride, so that the anti-infection effect is achieved, medicine resistance cannot be induced to be generated easily, and the application of sanguinarine hydrochloride has the advantage of being high in cure rate.

The invention discloses a traditional Chinese medicinal oral preparation for treating poultry salmonella infection and a preparation method thereof. The traditional Chinese medicinal oral preparation comprises the following traditional Chinese medicinal raw materials in parts by weight: 1-5 parts of liquorice, 1-5 parts of rhizoma atractylodis macrocephalae, 1-4 parts of root of pilose asiabell, 2-5 of poria, 2-5 parts of anemone chinensis, 1-2 parts of fructus forsythiae, 1-2 parts of cortex fraxini, 1-3 parts of coptis chinensis, 1-3 parts of scutellaria baicalensis, 1-3 parts of bupleurum chinense and 1-3 parts of dried rehmannia root. Compared with the prior art, the traditional Chinese medicinal oral preparation has the beneficial effects that the traditional Chinese medicinal oral preparation can effectively treat various diseases caused by salmonella infection and has the characteristics of quick effect, exact curative effect, no medicine residue and no toxic and side effects.

The invention relates to a product for preventing and / or treating salmonella infection, which belongs to the technical field of biological medicines. The product provided by the invention can inhibitthe growth of salmonella; the growth amount of salmonella is reduced by 90.4 percent; according to the product disclosed by the invention, the adhesion and invasion capabilities of salmonella can be reduced; the salmonella adhered to and invading HT-29 cells is obviously reduced; moreover, the product provided by the invention can inhibit the expression of salmonella virulence genes; the expression quantities of salmonella virulence genes InvA, AvrA, SsrB, HilA and SopD are respectively reduced by 69.3 percent, 62.4 percent, 57.4 percent, 53.4 percent and 31.3 percent; the inhibition effect isobviously superior to that of single use of ZS2058 or eugenol, so that the product provided by the invention has a strong effect of preventing and / or treating salmonella infection.

There is provided a vaccine composition comprising a combination of a genetic deletion mutant S. typhimurium microorganism and a genetic deletion mutant E. coli microorganism, suitable for mass application to poultry. Also provided is a safe and effective method to protect poultry against the ravages of E. coli and Salmonella infection and disease.

The invention discloses an application of n-3 polyunsaturated fatty acid in preparing a medicine for preventing salmonella infection, and belongs to the field of preventing salmonella infection. According to the invention, proved by an animal model of salmonella infection, the n-3 polyunsaturated fatty acid can obviously reduce the death rate, so that the survival rate of mice infected with salmonella is improved from 56% of a control group to 75%, and the survival rate is improved by 33%. In addition, the n-3 polyunsaturated fatty acid is low in price and wide in source, is a common nutritional supplement, is safe and stable, and has great health benefits, such as prevention of cardiovascular and cerebrovascular diseases, obesity, metabolic syndrome and the like; the n-3 polyunsaturated fatty acid can be supplemented during the salmonella outbreak period, but the infection symptom does not appear, to prevent the salmonella infection or slow down the disease course, the use of antibiotics is reduced, the negative effect caused by the antibiotics is reduced, the operation is easy, and the n-3 polyunsaturated fatty acid has good application prospect.

The present invention relates to a composition comprising bacteriophage SP-1, the bacteriophage capable of destroying Salmonella once being infected in Salmonella, as an active ingredient, and a method for prevention and treatment of Salmonella infection using the same. Bacteriophage SP-1, the active ingredient of the composition of the present invention, characteristically has the killing activity to Salmonella and has the genome represented by SEQ. ID. NO: 1.

The invention discloses ferroferric oxide nanoparticles as well as a preparation method and the application of the ferroferric oxide nanoparticles in inhibiting proliferation of Salmonella. The ferroferric oxide nanoparticles are added to a feed for resisting Salmonella infection in poultry. According to the invention, the ferroferric oxide nanoparticles are used for resisting the Salmonella infection of the poultry for the first time; the ferroferric oxide nanoparticles are added in the feed during the brooding period, so that the bacteria loading amount of the Salmonella in broiler bodies infected with the Salmonella can be significantly reduced, and the ferroferric oxide nanoparticles have favorable effects on liver tissues, do not produce side effects and have lower use cost, and further are convenient in use and simple in production process. According to the ferroferric oxide nanoparticles and the preparation method and the application of the ferroferric oxide nanoparticles in inhibiting the proliferation of the Salmonella, a new way for treating the Salmonella is opened, and a new research idea for the development of medicines resisting the Salmonella is further provided.

The invention discloses a salmonella bacteriophage with a wide lysis spectrum and high temperature resistance. The preservation number is CGMCC No.21995. After whole genes are sequenced, the salmonella bacteriophage does not have virulence genes and lyogenic genes. The salmonella bacteriophage RDP-SA-20049 acts for 60 min at the high temperature of 90 DEG C, the titer is kept at 1.0 * 10 < 7 > pfu / mL or above, and the salmonella bacteriophage RDP-SA-20049 has high biological activity and good high temperature resistance. The salmonella bacteriophage can be used for lysing pathogenic bacteria including 5 strains of salmonella typhimurium, 4 strains of salmonella pullorum, 1 strain of salmonella paratyphi B, 1 strain of salmonella typhimurium, 7 strains of salmonella enteritidis and salmonella BS-20036, and has the characteristic of high cracking rate, the cracking rate is up to 99%, the cracking performance is stronger, and the salmonella bacteriophage has good advantages in treatment of salmonella infection diseases, and is high in titer and good in prevention and treatment effect.

The invention relates to salmonella broad-spectrum lyase with in-vitro lytic activity and application thereof, and belongs to the technical field of biology. The lyase is named as LysMD19, an amino acid sequence of the lyase is shown as SEQ ID No. 1, and a nucleotide sequence of the lyase is shown as SEQ ID No. 2. The lyase can directly and efficiently lyse salmonella in-vitro under the conditionof no cell permeation reagents such as ETDA, the lysing effect is achieved on salmonella enteritidis, salmonella typhimurium, salmonella pullorum and other salmonella of different serotypes, good temperature stability and high acid and alkali resistance are achieved, the lyase can be used for preventing and treating salmonella infection, and also can be used for killing salmonella in multiple links such as source farms, processing, packaging and transportation to market selling, the safety 'from farms to dining tables' is ensured, and good application prospects and values are achieved.

A method for inhibiting or preventing Salmonella invasion of the intestinal epithelium in a subject, the method comprising enterally administering to the subject a pharmaceutically effective amount of a fatty acid dissolved or suspended in a pharmaceutically acceptable carrier, wherein the fatty acid contains 10 to 30 carbon atoms, wherein the fatty acid is more particularly an unsaturated fatty acid containing 1-4 carbon-carbon double bonds and / or 1 or 2 carbon-carbon triple bonds, or the fatty acid is more particularly a cis-unsaturated fatty acid, or the fatty acid is more particularly a cis-2-unsaturated fatty acid having the formula:wherein n is an integer of 6-26, and the fatty acid optionally includes a second carbon-carbon double bond resulting from removal of two hydrogen atoms on adjacent carbon atoms.

The invention provides an application of a drug composition in the preparation of drugs for preventing salmonella infection in laying hens, improving egg laying performance of the laying hens, reducing the amount of salmonella in shells and contents of eggs and improving quality of the eggs of the laying hens. The drug composition comprises, by weight, 20-50 parts of gallnut tannic acid and 10-30parts of glycine. The drug composition has the advantages of green safety and no tolerance by taking 'source conditioning' as the core; replacing antibiotics, the drug composition can effectively inhibit and kill salmonella, eliminate endotoxemia caused by bacterial death and release of endotoxin, adjust the body's immune defense, purify salmonella from the laying hens, reduce horizontal spread ofsalmonella and egg-borne contamination caused by potential endogenous displacement of chickens, improve egg laying performance and the quality of the eggs and effectively avoid food safety problems caused by the egg-derived salmonella.

The present invention relates to a composition comprising bacteriophage SP-1, the bacteriophage capable of destroying Salmonella once being infected in Salmonella, as an active ingredient, and a method for prevention and treatment of Salmonella infection using the same. Bacteriophage SP-1, the active ingredient of the composition of the present invention, characteristically has the killing activity to Salmonella and has the genome represented by SEQ. ID. NO: 1.

The invention discloses a chicken ring RNA Chr26:2670958 / 2679178 detection primer, a method and application. The primer is prepared from a chicken ring RNA Chr26: 2670958 / 2679178 annular upstream primer body and a chicken ring RNA Chr26: 2670958 / 2679178 annular downstream primer body, wherein nucleotide sequences of the chicken ring RNA Chr26: 2670958 / 2679178 annular upstream primer body and the chicken ring RNA Chr26: 2670958 / 2679178 annular downstream primer body are respectively shown in SEQ ID NO.1 and SEQ ID NO.2. By means of the method disclosed by the invention, the relative expressionsituation of the chicken ring RNA Chr26: 2670958 / 2679178 can be accurately detected after a chicken is infected by salmonella enteritidis; the relative expression situation is utilized as a molecularmarker to identify salmonella infection resistance individuals; thus, theoretical basis and scientific basis are provided for salmonella enteritidis infection resisting genetic breeding of chickens.

The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.

The invention discloses an enterococcus faecium YQH2, and further discloses a probiotic preparation. The probiotic preparation comprises the enterococcus faecium YQH2. The invention also discloses application of the enterococcus faecium YQH2 or the probiotic preparation in breeding of poultry animals. The invention also discloses application of the enterococcus faecium YQH2 or the probiotic preparation in preparation of medicines for repairing intestinal injury or enhancing intestinal immunity. The invention further discloses application of the enterococcus faecium YQH2 or the probiotic preparation in preparation of amedicines for inhibiting salmonella typhimurium infection. The enterococcus faecium YQH2 disclosed by the invention can inhibit the growth of pathogenic bacteria. The enterococcus faecium YQH2 can improve the crypt depth of salmonella infection and increase of an inflammatory factor level, stimulates proliferation of intestinal mucosa epithelial cells and has the potential of maintaining intestinal mucosa barriers.

The present invention provides a vaccine composition, which contains a combination of Salmonella typhimurium microorganisms of gene deletion mutants and Escherichia coli microorganisms of gene deletion mutants, and is suitable for mass application to poultry. The invention also provides a safe and effective method for protecting poultry from Escherichia coli and Salmonella infection and disease damage.

The invention discloses a screening method of feed probiotics capable of preventing porcine salmonella infection. The screening method comprises the following steps: screening piglets infected by salmonella and disease resistant piglets, collecting intestinal content samples of piglets infected by salmonella and disease resistant piglets; carrying out high-throughput sequencing on intestinal microbial floras of piglets infected by salmonella and disease resistant piglets; performing meta-genomic comparison and analysis on intestinal microbes of piglets infected by salmonella and disease resistant piglets; and screening and identifying probiotics capable of preventing salmonella infection. The provided screening method screens out probiotics capable of preventing salmonella infection from agreat amount of microbial floras of disease resistant piglets, and is strongly targeted, the blindness of screening is avoided, the screening efficiency is improved, and a large amount of labor, materials, and time is saved.

The present invention relates to Salmonella mutant strains and their use as a vaccine for preventing Salmonella infection, in particular in eggs.

The invention discloses a caenorhabditis elegans infection model for high-throughput rapid detection of salmonella virulence and a preparation method and application thereof, and belongs to the technical field of biology. The model is characterized in that: the caenorhabditis elegans is sek-1, glp-1 gene defect type caenorhabditis elegans; the culture medium for the salmonella infected caenorhabditis elegans is an S-medium buffer solution with a pH value of 7.0 + / -0.2; and the infection concentration of the salmonella is 10 < 7 >-10 < 11 > CFU / mL. The survival rates of the nematodes are obviously different when the nematodes are infected at the second to the fourth time, and the pathogenicity is preliminarily judged. Therefore, by establishing a salmonella infected caenorhabditis elegans model, the salmonella virulence can be quickly, simply, conveniently and efficiently detected, and a theoretical basis is provided for salmonella pathogenesis research and related drug screening.

The invention relates to a colistin sulfate and berberine hydrochloride composition and a preparation method of pellets of the colistin sulfate and berberine hydrochloride composition and aims to solve the problems that the effect of treating escherichia coli and salmonella infection by using the colistin sulfate is poor due to poor intestinal absorption in the prior art, and the use dosage is required to be increased for reaching an ideal treatment effect. The preparation method comprises the following steps: firstly, mixing the colistin sulfate, the berberine hydrochloride, a filling agent, a lubricating agent and a disintegrating agent; secondly, crushing to obtain mixture powder; adding a binding agent to obtain pellets; coating to obtain the pellets of the colistin sulfate and berberine hydrochloride composition. The pellets of the colistin sulfate and berberine hydrochloride composition, obtained by the preparation method disclosed by the invention, are prepared by adopting conventional equipment such as a granulating device and a coating device in the pharmaceutical industry; the preparation method can realize continuous production and is suitable for industrial production; high yield is realized.

The invention provides a plant Lactobacillus (Lactobacillus plantarum) resistant to Salmonella enteritidis infection and application thereof, belonging to the technical field of microbes. The bacterium was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms on September 24, 2015, and the preservation number is CGMCC No.11446. The Lactobacillus plantarum CGMCC No. 11446 of the present invention has better properties of acid resistance and bile salt resistance, and has significant inhibitory effect on the growth of Salmonella enteritidis. It has a strong inhibitory effect on the adhesion of Salmonella Enteritidis to intestinal epithelial cells HT-29, and has the effect of preventing or alleviating Salmonella Enteritidis infection. The Lactobacillus plantarum CGMCC No. 11446 can be used to prepare pharmaceutical compositions for preventing or alleviating Salmonella enteritidis, food and fermented food containing active lactic acid bacteria, and has very broad application prospects.

The invention relates to an application of sophora alopecuroide total alkali in preparation of a traditional Chinese medicine preparation for treating chicken pullorum as well as the traditional Chinese medicine preparation and a preparation method thereof. The traditional Chinese medicine preparation is used for treating salmonella infection of the chicken pullorum. The traditional Chinese medicine preparation consists of the sophora alopecuroide total alkali and auxiliary materials; and the preparation method comprises a step of preparing the sophora alopecuroide total alkali and auxiliary materials into powder or granules by virtue of a preparation process, and the prepared preparation is clinically used for treating the chicken pullorum by virtue of drinking water or stirring. The preparation provided by the invention does not have toxic or side effects and is simple in processing technology and low in production cost, and pharmacodynamics researches show that the preparation provided by the invention has the effects of preventing and treating salmonella of the chicken pullorum, and does not have antibiotic residues after being taken.