31 results about "Sporidiobolus salmonicolor" patented technology

The invention aims to provide bacillus subtilis WL-04 and application thereof to livestock breeding. The preservation number of the bacillus subtilis WL-04 is CCTCC NO:M 2013343. The bacillus subtilis WL-04 can be used for remarkably inhibiting the growth of Escherichia coli, salmonella, clostridium pefringens and other conditional pathogenic bacteria of the intestinal canal of livestock, and particularly, the bacteriostasis rate of the bacillus subtilis WL-04 to Escherichia coli and clostridium perfingens is above 75%; the enzyme production is stable; the average activity of alpha-amylase is 22U / mL, and the average activity of neutral protease is 170U / mL; the reproduction of probiotics of intestinal canal can be promoted; high temperature for pelletizing can be endured; the influence of inverse environments with low-pH gastric fluid and high-concentration cholate is endured; the production performance of breeding animals can be effectively improved; immune organ development can be promoted. The bacillus subtilis WL-04 can be widely applied to livestock feeds as a feed additive.

The invention discloses bacillus subtilis AP139 and fermented microbial inoculum thereof, and an application method of the bacillus subtilis AP139 and the fermented microbial inoculum. The bacillus subtilis is preserved in the China Center For Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M2014053. The bacterial strain can restrain the growth and the propagation of porcine toxigenic pasteurella multocida in a culture dish experiment by screening porcine toxigenic pasteurella multocida resistance. The bacillus subtilis AP139 is atomized in an animal breeding shed, the bacillus subtilis AP139 has an obvious characteristic of restraining the growth of pasteurella multocida in air, the pasteurella multocida attack rate in a breeding farm is reduced, at the same time, the bacillus subtilis AP139 also has a good effect on restraining escherichia coli, staphylococcus aureus and salmonella, and the bacillus subtilis AP139 has a favorable application prospect.

The invention provides Lactobacillus salivarius M6, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the number of CGMCC 3505. The invention also provides a lactobacillus which can be mixed with animal feed or added into water, can be used together with antibiotics at the same time, and has antibiotic resistance. The strain can inhibit growth of Salmonella enterica, Escherichia coli and other pathogenic bacteria. The antibacterial composition of the Lactobacillus salivarius M6 can be used in drinking water, drinking water additives, feed and food additives of animals, human and animal medical composition, as well as drinks, drinking additives, foods, food additives and the like of humans. By implementation of the invention, pathogenic bacterium infection of animals or humans can be effectively prevented and treated, and the number of intestinal probiotics can be increased or maintained, so that the growth of pathogenic bacteria is inhibited, the dosage of antibiotics is reduced, and the aim of preventing disease infection of animals and humans can be achieved.

The invention relates to application of eugenol and in particular relates to application of eugenol in food-borne pathogenic bacterium in-vitro inhibition, culture mediums of food-borne pathogenic bacterium are TSA culture mediums or TSB culture mediums with pH values being 5.0, 6.0 and 7.0; the diameter of an inhibition zone is measured for judging antibacterial activity of eugenol; and a multiple dilution method is adopted for carrying out minimal inhibitory concentration (MIC) determination on various bacteria. The application of eugenol in the food-borne pathogenic bacterium in vitro inhibition has the advantages that escherichia coli, staphylococcus aureus, salmonella and shigella are taken as research objects, MIC of 2-methoxy-4-(2-propenyl) phenol on common pathogens in food is researched, influence of pH on antibacterial effect of 2-methoxyl-4-(2-propenyl) phenol is studied, and application value of 2-methoxy-4-(2-propenyl) phenol in food antisepsis aspect is researched; and application range of 2-methoxy-4-(2-propenyl) phenol can be beneficially expanded.

An eurotium cristatum LS1 strain is classified and named as EurotiumCristatum LS1 and preserved in China Center for Type Culture Collection on June 17, 2015, and the preservation number is CCTCC NO:M 2015379. The strain LS1 can grow on kudzuvine roots and conduct bioconversion, when the strain LS1 is used for fermenting kudzuvine roots, an obtained fermenting product has stronger inhibiting effects on cowpea anthracnose, tomato early blight, sclerotinia sclerotioru and Chinese cabbage leaf spots, and the inhibiting effects on five putrefactive bacteria of staphylococcus aureus, bacillus cereus, escherichia coli, bacillus subtilis and salmonella are all improved than those before fermentation is conducted. It is shown that a product obtained by fermenting kudzuvine roots through the strain LS1 has broad-spectrum antibacterial activity, can inhibit pathogenic fungi and the putrefactive bacteria to some extent, is expected to be developed into biopesticides to prevent and control crop diseases and is expected to serve as a food sterilizing agent to be used for food preservation.

The invention belongs to the technical field of culture of lactic acid bacteria, and relates to lactobacillus plantarum for feeding, and a culture method and application of the lactobacillus plantarum. The lactobacillus plantarum WEI-70 with a preservation number of China General Microbiological Culture Collection Center (CGMCC) No. 11747 is separated from feces of healthy piglets which are 60 days old, can tolerate pig bile salts with low pH values and high concentrations, and has a higher inhibition effect for escherichia coli, salmonella, streptococcus and staphylococcus aureus; the inhibition zone of the lactobacillus plantarum WEI-70 can reach 30-35mm. The high density liquid culture method of the lactobacillus plantarum WEI-70 with the preservation number of CGMCC No. 11747 is obtained by optimizing the formula, culture temperature and optimal fermentation initial pH value of a culture medium; the maximum viable number of fermentation liquor can reach 6.0*10<9> CFU / mL and is not less than six times the viable number obtained by using an MRS culture medium under the same culture conditions. The thalli of the fermentation liquor are collected by using a high speed tubular centrifuge, are then mixed with a special protective agent according to a certain ratio and are subjected to vacuum freezing-drying; the maximum viable number of the obtained freeze-dried bacteria powder can reach 7.0*10<11> CFU / g, and the survival rate of the freeze-dried bacteria powder can reach 93%.

The invention discloses a lactobacillus salivarius strain and application thereof. The lactobacillus salivarius strain (with number of HVRIC5) is obtained by carrying out bacteria reproduction and enrichment on wild chicken manure in an MRS liquid selective medium and carrying out isolated culture and inoculation and purification. The microbial collection number of the strain is CGMCC No.6787. Lactobacillus salivarius has stronger acid and bile salt resistance. Antibacterial experiments in vitro indicate that the strain has stronger antibacterial effects on Gram-negative escherichia coli, salmonella and Gram-positive staphylococcus, shows a broad-spectrum antibacterial effect, has the effect on promoting growth of SPF (specific pathogen free) chickens, can effectively resist infection with salmonella and avian infectious laryngotracheitis viruses when being fed to the SPF chickens and has the effect of effectively protecting the SPF chickens.

The invention discloses a pichia pastoris expressing a recombinant Thanatin antibacterial peptide. The recombinant Thanatin antibacterial peptide comprises the amino acid sequence of GSKKPVPIIYCNRRTGKCQRM. The pichia pastoris is used for the secretory expression of Thanatin, large-scale expression of Thanatin is realized, and production cost is reduced. By the adoption of the pichia pastoris production strain, the highest expression level of Thanatin reaches 0.6 g / L, the highest level in public reports; besides, a Thanatin antibacterial peptide exists in a fermentation supernatant, there is noneed to break cells, and a Thanatin sample containing few impure proteins can be obtained simply through filtration to remove thalli, which facilitates subsequent separation and purification of Thanatin and reduces production cost. The prepared Thanatin sample has a significant bacteriostatic effect on escherichia coli and salmonella.

The invention discloses a yeast strain Sporidiobolus salmonicolor AH3 for producing a biosurfactant. The microbial collection number of the yeast strain is CGMCC No. 4814. The yeast strain provided by the invention can grow with C10-C24 alkane and / or polycyclic aromatic hydrocarbons as the carbon source, and the biosurfactant is generated. When the yeast strain is cultured with alkane and polycyclic aromatic hydrocarbons as the carbon source, emulsifying ability of the generated biosurfactant is enhanced. The yeast strain Sporidiobolus salmonicolor AH3 CGMCC No.4814 has good effects of degrading and emulsifying hydrophobic organic matters, and has a good application prospect in treatment of petroleum produced wastewater.

The invention belongs to the field of biotechnology, and particularly relates to a donkey-derived Salmonella AD19 strain and application thereof. The microbial preservation number is CGMCC N0.18046. The preservation date is June 27, 2019. The preservation unit is: China General Microbiological Culture Collection Center. According to the invention, a gram-negative bacillus is isolated and screenedfrom dead foal disease material collected from a donkey farm in Shandong Province. After purified and cultured, the isolated strain was identified as Salmonella by morphological identification, biochemical identification and cloning and sequence analysis of 16SrDNA. The results of pathogenicity tests show that the Salmonella strain has certain lethality to mice and most notably can cause abortionof pregnant mice. This strain can be used to prepare an inactivated vaccine of Salmonella causing donkey abortion. The inactivated vaccine prepared from the strain has good immunogenicity, high safetyand good protection effects.

The present invention is related with the Microbiology field and particularly with a composition and a method for early detection, identification, differentiation and count of microscopic organisms, concretely Gram-negative microorganisms. The composition described in the invention consist on a mixture of substances of protein origin with a total nitrogen content from 9 to 20% and in relationship between 2:1 to 24:1, concerning to the content of inhibitors of the Gram-positive organisms. It contains a mixture of organic and inorganic substances that facilitate the differentiation of the Gram-negative organisms, being this mixture in a relationship from 0.5:1 to 2:1 concerning to the mixture of substances of protein origin. The referred composition allows the detection and differentiated count of E. coli and other coliform organisms due to the blue-greenish color of the colonies of these microorganisms on the orange bottom of the medium; Salmonella not typhi for the red color of the centers of the colonies on rosy bottom of the medium; Salmonella typhi and Proteus for the transparency of the colonies; Citrobacter and Klebsiella for the violet color of the colonies on the pink to orange bottom of the medium and Pseudomonas aeruginosa for the orange color with darker center of the colony, taking greenish pigmentation after 24 hours and producing greenish fluorescence under low ultraviolet light.

The invention relates to a bacilli, its compound preparation and the preparing method for the compound preparation. Its name is Bacillus subtilis LY-35, which is preserved in the general microbe center of Micro Germ conservation Management committee of China, and its conservation number is:CGMCC N0.1222. The preparation is a mixture of lambda phage of culture of bacilli and staphylococcus, streptococcus, colibacillus, bacillus proteus, pseudomonad, salmonella, pasteurella and klebsiella, can cure and prevent all kinds of illness caused by conditional pathogen,is a micro biological preparation which can be used by being mixed with antibiotics or replace antibiotics without any remainder and pollution, with good curative effect and safety.

The invention belongs to the technical field of livestock breeding and specifically relates to application of a pink plumepoppy herb regulator capable of improving weaned pig immune performance. The application comprises the following steps: A1, a bacteriostatic test; A2, an animal feeding test; A3, a cell test. The pink plumepoppy herb regulator has an obvious bacteriostatic effect on pathogenicmicroorganisms, the pink plumepoppy herb regulator has an bacteriostatic effect on gram positive bacteria or gram negative bacteria, and an antibacterial spectrum testing result of the pink plumepoppyherb regulator to the pathogenic microorganisms is staphylococcus aureus larger than bacillus subtilis larger than escherichia coli larger than salmonella. When being applied, the pink plumepoppy herb regulator has the bacteriostatic effect on all weaned pig enteropathogenic microorganisms like the escherichia coli, the salmonella and the staphylococcus aureus and especially has the most remarkable bacteriostatic effect on the staphylococcus aureus.

The present invention provide a composition of non-toxic low-cost ingredients that effectively kill pathologic bacteria and methods for use of the composition. The present invention comprises novel compositions and methods for controlling enteric pathogens and spoilage organisms such as Salmonella, Escherichia, Campylobacter, Listeria, Pseudomonas and Enterobacteracae on the surface of meat products and food preparation surfaces.

Disclosed are a sulfate reducing bacterium with a polyacylamide degradation function and applications of the bacterium. The invention relates to a microorganism and applications of the microorganism. The invention firstly put forward the sulfate reducing bacterium with the polyacylamide degradation function, the bacterium is named as Salmonella Daqing MF, namely, Salmonella Daqing MF (preservation code: CGMCC No.1910), preserved in the China General Microbiological Culture Collection Center, and belonging to the Salmonella Genera and the Salmonella Daqing Species in taxonomy. The bacterium belonging to the Gram negative short-bacilli is covered by a capsule and is obligate anaerobic, variable in size bound in 0.35Mu-0.7Mum width multiplied by 0.7Mum-1.6Mum length, without flagella on the bacterium body; when growing on a solid medium, the bacterium develops into a black round colony, smooth in surface and irregular in edge.

The invention relates to an on-site fermented bacillus licheniformis preparation for aquaculture farm and applications thereof. The preparation comprises the following raw materials: bacillus licheniformis powder, colistin sulfate, natamycin, inosine, corn flour, corn steep liquor powder, sodium chloride, serine, fructose, and brown sugar. The colistin sulfate and natamycin in the preparation can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (inosine, fructose, and serine) can rapidly promote the germination of spores of bacillus licheniformis and improve the germination rate of spores. The provided preparation can be applied to the onsite fermentation and propagation in a simple and crude aquaculture farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The preparation can improve the water quality and intestinal functions, prevent disease, promote growth, increase output, and improve product quality.

The invention belongs to the field of food, especially belongs to the field of pickled vegetable fermentation biotechnology and particularly relates to a micro-ecological additive for low-salt pickled vegetables and a preparation method of the micro-ecological additive. The low-salt pickled vegetable micro-ecological additive comprises lactobacillus pentosus which is classified and named as Lactobacillus pentosus PCYSWX-5 and preserved in the China General Microbiological Culture Collection Center on July 15th, 2016, and the preservation number of the lactobacillus pentosus is CGMCC No. 12793. The prepared micro-ecological additive has the advantages that the micro-ecological additive can inhibit or kill common pathogenic bacteria such as escherichia coli, staphylococcus aureus, enterobacter sakazakii, listeria monocytogenesis and salmonella, does not have an inhibition effect on main fermentation bacteria, and can increase the eating safety of the pickled vegetables; the additive can intensely inhibit or kill nitrate reducing bacteria in the low-salt pickled vegetables in the initial stage of fermentation to allow the nitrate content in the low-salt pickled vegetables to be lower than national standards.

The invention discloses a strain of Lactobacillus plantarum ZN-3 and its application. The Lactobacillus plantarum ZN-3 provided by the invention is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is: CCTCC NO: M 2017286. The Lactobacillus plantarum ZN-3 provided by the present invention has the characteristics of acid resistance and bile salt resistance, can colonize the intestinal tract through the stomach, and has antibacterial effect on animal-derived pathogenic bacteria, including Gram-negative bacteria: large intestine bacillus, Salmonella, Pastobacterium multocida, Erysipelothrix suis, Haemophilus parasuis, porcine infectious pleuropneumonia, also includes Gram-positive bacteria: Streptococcus suis, Staphylococcus aureus, Staphylococcus epidermidis. Compared with antibiotics, it has a good antibacterial effect on pathogenic bacteria with strong drug resistance.

Salmonella enteric serotype Typhimurium is a causative agent for gastroenteritis. It is the leading cause of hospitalization and death caused by a food-borne pathogen in the US. As is the case with many gram- negative pathogens, Salmonella spp. use type III secretion systems (T3SS) to deliver proteins to host cells to induce infections. The T3SS uses a molecular syringe and needle, the type III secretion apparatus (T3SA), as a conduit for the transport of the T3SS proteins from the bacteria to the host. Because proteins associated with the tip of the T3SA are extracellular, they are potential protective subunit vaccine candidates against Salmonella. These pathogens also have a T3SS that is involved with intracellular escape from the vacuole with an associated T3SA. Within this invention, we begin to show proof of concept that these proteins are protective.

There is a process which can pasteurize in-shell chicken eggs to inactivate pathogens when present which includes all strains of salmonella and all strains of viruses that historically have been known to exist within chicken eggs and currently are known to be evolving into new and separate strains which may cause large quantities of human illnesses unless countermeasures are developed and employed. One such countermeasure is provided through pasteurization of the subject in-shell eggs through pasteurization involving concurrently a secured environment together with a protocol which enables total inactivation of the targeted pathogens whether bacterial or viral without risk of recontamination.

The invention discloses a yeast strain Sporidiobolus salmonicolor AH3 for producing a biosurfactant. The microbial collection number of the yeast strain is CGMCC No. 4814. The yeast strain provided by the invention can grow with C10-C24 alkane and / or polycyclic aromatic hydrocarbons as the carbon source, and the biosurfactant is generated. When the yeast strain is cultured with alkane and polycyclic aromatic hydrocarbons as the carbon source, emulsifying ability of the generated biosurfactant is enhanced. The yeast strain Sporidiobolus salmonicolor AH3CGMCC No.4814 has good effects of degrading and emulsifying hydrophobic organic matters, and has a good application prospect in treatment of petroleum produced wastewater.

The invention provides a composite bacteria preparation used for disinfection. The composite bacteria preparation is composed of, by mass, 1 to 25% of a microbial community, 0.5 to 10% of a microbial nutrient solution, and 65 to 95% of bacteria normal saline. The microbial community with bacteria killing effect is taken as a main microbial community, the content of effective probiotics is capable of reaching 3*10<7> cfu / g; the main microbial community possesses killing, phagocytosis, and rapid lysis effect on pathological bacteria such as vibrio parahaemolyticus, aeromonas hydrophila, aeromonas punctata, escherichia coli, salmonella, and edwardsiella spp, so that the composite bacteria preparation is capable of reducing the content of pathological bacteria in water bodies obviously, killing bacteria with bacteria, and repairing water body microecological balance.

The invention belongs to the technical field of research and development of medical products. The invention relates to a preparation process and application of ethanol extracts of Parasenecio firmus Kom. leaves. The preparation process comprises the following steps of: weighing dry coarse powder of Parasenecio firmus Kom. leaves, extracting the coarse powder with 70% of ethanol and filtering the extracts to obtain filtrate and filter residues; repeatedly extracting the filter residues with 70% of ethanol twice; and mxing the obtained filtrate, concentrating the merged filtrate under reduced pressure and storing the concentrate in a refrigerator at 4 DEG C for later use. The products are used for preparing the pharmaceutical preparations for inhibiting escherichia coli, streptococcus, salmonella, pasteurella and staphylococcus aureus.

There is a process which can pasteurize in-shell chicken eggs to inactivate pathogens when present which includes all strains of salmonella and all strains of viruses that historically have been known to exist within chicken eggs and currently are known to be evolving into new and separate strains which may cause large quantities of human illnesses unless countermeasures are developed and employed. One such countermeasure is provided through pasteurization of the subject in-shell eggs through pasteurization involving concurrently a secured environment together with a protocol which enables total inactivation of the targeted pathogens whether bacterial or viral without risk of recontamination.

The present invention discloses a method for processing oil crops by using rhodotorula and belongs to the technical field of fermentation. The method is as follows: rhodotorula of rhodotorula mucilaginosa, sporidiobolus salmonicolor, rhodotorula glutinis, etc., capable of producing carotenoids are inoculated in an oil-enriched oil crop fermentation substrate for solid-state fermentation to obtainoil-enriched oil and oil crop meal; the rhodotorula fermentation metabolite carotenoids have biological activities of preventing oxidation, preventing vascular sclerosis, enhancing immunity, preventing cancers, etc., thus the carotenoid content of the oil and oil crop meal obtained by using the method can be respectively as high as 9.071 [mu]g / g and 8.062 [mu]g / g; and the method only needs one-time fermentation and one-time oil squeezing, does not contain additional functional substances, can obtain the carotenoid-enriched oil and oil crop meal at the same time, and greatly reduces productioncost of functional oils and fermented oil crops.

The present invention relates to a new cocktail of bacteriophages with specific lytic activity against Salmonella enteritidis, Salmonella typhimurium and Salmonella paratyphi B., for reducing, eliminating and / or preventing them in farm animals and animals from the poultry sector, such as poultry, hens and breeding hens, in addition to eggs. It may be administered as an additive in the feed, in water or by spray. Moreover, the cocktail may be used as a disinfectant in work areas of farms and abattoirs, and in processed foods, without affecting the organoleptic properties of the product.

Method for producing multiple modifications in the chromosome of Gram-negative bacteria and Salmonella strains which are deficient in c-di-GMP synthesis obtained by said method. The method can be used to make multiple modifications in the genome of Gram-negative bacteria, simply and efficiently, using the plasmids of the invention, which comprise a marker gene under the control of a constitutive promoter, a replication origin specific for Gram-negative bacteria, a gene which encodes a heat-sensitive protein essential for initiating replication of the plasmid, making the replication origin heat-sensitive, and a counter-selection gene. The invention also relates to mutant Salmonella enterica strains obtained using the method of the invention in which some of the twelve genes which encode proteins with GGDEF domain have been selected*, and to the use thereof as expression vectors, immunotherapeutic agents and in metabolic studies

The present invention belongs to the field of food, especially the field of pickled vegetable in food, and specifically relates to a method for controlling biological safety of pickled vegetable. According to the method, the amounts of each strain in pathogenic bacteria are controlled at the concentration of 10<6>-10<7> CFU / g, placing is performed at a room temperature, and the viable counts of the pathogenic bacteria are respectively detected in the third day and the sixth day, wherein the pickled vegetable product has good microbiological safety if the viable count is reduced to not higher than 0.1% of the initial concentration in the third day and in the sixth day, Salmonella, Staphylococcus aureus and Listeria monocytogenes disappear and the viable count of the Escherichia coli is less than or equal to 30 CFU / g, and otherwise, the risk of pathogenic bacteria contamination exists in the pickled vegetable product. With the method of the present invention, the microbiological safety of the pickled vegetable product can be simply and effectively evaluated, the steps are simple, and the experiment time is substantially shortened.

The invention relates to an on-site fermented bacillus licheniformis preparation for aquaculture farm and applications thereof. The preparation comprises the following raw materials: bacillus licheniformis powder, colistin sulfate, natamycin, inosine, corn flour, corn steep liquor powder, sodium chloride, serine, fructose, and brown sugar. The colistin sulfate and natamycin in the preparation can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (inosine, fructose, and serine) can rapidly promote the germination of spores of bacillus licheniformis and improve the germination rate of spores. The provided preparation can be applied to the onsite fermentation and propagation in a simple and crude aquaculture farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The preparation can improve the water quality and intestinal functions, prevent disease, promote growth, increase output, and improve product quality.