The invention belongs to the field of 
biotechnology, and in particular relates to a development method of 
fluorescent protein transgenic zebra fish capable of highly sensitively monitoring 
water body PAHs by utilizing the 
cascade amplification effect. According to the method, a UAS-Gal4 
gene expression regulation 
system is taken as a basis, a multiring 
aromatic hydrocarbon response element AhREs after optimization and reorganization is utilized for driving the expression of the upstream 
plasmid Ga14, and further, the expression of the fluorescent 
gene eGFP in the downstream UAS 
plasmid is regulated; meanwhile, the TALE-TF transcriptional activation enhancement technology is innovatively combined, the expression quantity of the 
eGFP gene is further enhanced by utilizing the TALE transcriptional activation effect, and then the purpose of increasing the 
fluorescence signals through multiple ways is achieved; finally, the low-copy induced 
fluorescent protein transgenic zebra fish with stable inheritance is obtained by utilizing the I-SceI wide-range 
nuclease transfer system, and the influences on the 
toxicity of the zebra fish are small; by manufacturing the 
fluorescent protein transgenic zebra fish 
highly sensitive to the environmental pollutants, the toxic pollutants in the 
water body can be easily, efficiently, sensitively and intuitively monitored.