57 results about "Fisetin" patented technology

Formulations and methods of treatment and putative prevention for aging (anti-aging composition) and for diseases or conditions of all reactive oxygen species-dependant illnesses, such as Alzheimer's disease, Parkinson's disease, diabetes mellitus, cardiovascular disease, cancer, hepatitis, and disorders associated with estrogen deficiencies including osteoporosis and breast cancer and for improving athletic performance of humans include resveratrol and two (2) or more of the following features or additional active ingredients: (1) slow release formulation of resveratrol; (2) pterostilbene; (3) quercetin; (4) fisetin, and (5) naringenin. Slow release is defined for the purposes of the present invention as releasing 95% of the active agent or agents in eight (8) hours through normal human gastrointestinal absorption.

A dietary supplement system includes a dietary supplement composition for oral administration by an individual in the morning, the composition, including (a) a telomere maintenance complex including: Purslane extract (aerial parts); Turmeric rhizome extract (95% curcuminoids); Quercetin dehydrate, Cayenne pepper fruit; Vanadium (as vanadyl sulfate); Fenugreek seed; Astragalus root extract, Omega fatty acid complex including linoleic acid; alpha-linolenic acid; oleic acid borage seed oil gamma-linolenic acid), evening primrose oil fish body oil (eicosapentaenoic acid; docosahexaenoic acid); (b) a calorie restriction mimetics and gene expression complex including Trans-resveratrol (from Polygonum cuspidatum root extract); Pterostilbene Fisetin 50% (Buxus microphlla Sieb (stem and leaf; Alpha lipoic acid, Coenzyme Q-10, Betaine HCl, Sulfur (from methylsulfonylmethane); L-Carnitine tartrate; L-Carnitine HCl, and (c) a free radical scavenger complex, including Green tea leaf extract catechin and polyphenols); Anthocyanins (from bilberry fruit and grape skin extracts).

The NF-E2-related factor 2 (Nrf2) is a key transcriptional regulator of antioxidant defense and detoxification. To directly monitor stabilization of Nrf2 we fused its Neh2 domain, responsible for the interaction with its nucleocytoplasmic regulator, Keap1, to firefly luciferase (Neh2-luciferase). It is shown herein that Neh2 domain is sufficient for recognition, ubiquitination and proteasomal degradation of Neh2-luciferase fusion protein. The novel Neh2-luc reporter system allows direct monitoring of the adaptive response to redox stress and classification of drugs based on the time-course of reporter activation. The novel reporter was used to screen a library of compounds to identify activators of Nrf2. The most robust and yet non toxic Nrf2 activators found—nordihydroguaiaretic acid, fisetin, and gedunin-induced astrocyte-dependent neuroprotection from oxidative stress via an Nrf2-dependent mechanism.

The invention relates to a health care composition for delaying senility, which is prepared from the constituents of resveratrol 40-350mg, fisetin 40-350mg, butein 40-350mg, quercetin 70-200mg, phosphatidy lcholine 80-1500mg, phosphoinositide 10-100mg.

The present disclosure generally relates to nutritional compositions that are suitable for administration to adult and pediatric subjects that include a neurologic component. The neurologic component may include a flavonoid compound, such as kaempferol, fisetin, or both, and provides beneficial health benefits such as enhanced brain development and improved memory, cognition, hand-eye coordination, and enhanced focusing. Furthermore, the neurologic component may act synergistically with other brain nutrients that may be present in the compositions.

The invention relates to a process for extracting fisetin from cotinus coggygria. The process comprises the following steps: (1) crushing anacardiaceae plant, namely, cotinus coggygria raw material, and then adding ethanol to preprocess; (2) ultrasonically extracting, filtering, and performing pressure-reduction concentration for the filtrate; (3) absorbing concentration liquid through macroporous resin, washing with water to remove impurity, eluting with ethanol, and collecting the eluant; (4) concentrating and drying the eluant, and separating and purifying through a high-speed counter current chromatograph; and (5) crystallizing. The process has the advantages that the process is simple, the operation is safe and convenient, and the process is suitable for the industrial production.

The invention discloses epimedium source galactosyl transferase and an application of the epimedium source galactosyl transferase to the preparation of hyperoside, and belongs to the technical field of gene engineering and biological medicines. The invention provides galactosyl transferase and an application of recombinant saccharomyces cerevisiae expressing the galactosyl transferase to the catalytic synthesis of flavonoid compounds. The constructed recombinant saccharomyces cerevisiae can be enabled to catalyze the transfer of UDP-galactose to various flavone substrates such as quercetin, kaempferol, myricetin, dihydroquercetin, dihydrokaempferol, dihydromyricetin, fisetin, morin and icaritin.

The present invention provides neuroprotective polyphenol compounds, which can be synthetic analogs of fisetin, baicalein or chlorogenic acid, that maintain neuroprotective, anti-inflammatory, glutathione promoting, and / or antioxidant properties. The neuroprotective polyphenol compounds are useful for promoting, enhancing and / or increasing neuron protection, growth and / or regeneration. The polyphenol compounds further find use for increasing and or maintaining intracellular glutathione (GSH) levels. The polyphenol compounds are also useful for treating, preventing, mitigating and / or delaying neurodegenerative conditions, including diabetes, Parkinson's disease, Huntington's disease, Alzheimer's disease, non-Alzherimer's dementias, multiple sclerosis, traumatic brain injury, spinal cord injury or ALS.

The invention relates to a method for screening compounds capable of improving generation efficiency of induced pluripotent stem cells, in particular to a method for screening compounds capable of improving generation efficiency of induced pluripotent stem cells in basis of organism lifetimes, particularly fruit fly lifetimes. The invention further relates to a lifetime prolonging compound, in particular to a fruit fly lifetime prolonging compound, and particularly discloses new usages of rapamycin, PP242, PQ401, resveratrol, fisetin, spermidine, LY294002 and curcumin in improvement of generation efficiency of induced pluripotent stem cells.

The invention relates to a preparation method of fisetin capable of inhibiting tyrosinase activity through rapid separation in a medium-pressure column, and belongs to the fields of development and applications of plant extract, natural drugs, and cosmetics. Fisetin with purity more than 95% can be obtained through the following steps: taking lacquer tree wood powder as the raw material, extracting the wood powder, and subjecting the extract to a middle-low pressure chromatogram and HPLC (high performance liquid chromatogram). The component obtained from lacquer tree can inhibit the tyrosinase activity efficiently, and the inhibition rate on the tyrosinase activity is greater than 95% when the concentration of the component is 150 [mu]g / mL or more. The preparation is simple and fast, and thus the provided method can be used to produce a natural tyrosinase inhibitor.

A dietary supplement system includes a dietary supplement composition for oral administration by an individual in the morning, the composition, including (a) a telomere maintenance complex including: Purslane extract (aerial parts); Turmeric rhizome extract (95% curcuminoids); Quercetin dehydrate, Cayenne pepper fruit; Vanadium (as vanadyl sulfate); Fenugreek seed; Astragalus root extract, Omega fatty acid complex including linoleic acid; alpha-linolenic acid; oleic acid borage seed oil gamma-linolenic acid), evening primrose oil fish body oil (eicosapentaenoic acid; docosahexaenoic acid); (b) a calorie restriction mimetics and gene expression complex including Trans-resveratrol (from Polygonum cuspidatum root extract); Pterostilbene Fisetin 50% (Buxus microphlla Sieb (stem and leaf; Alpha lipoic acid, Coenzyme Q-10, Betaine HCl, Sulfur (from methylsulfonylmethane); L-Carnitine tartrate; L-Carnitine HCl, and (c) a free radical scavenger complex, including Green tea leaf extract catechin and polyphenols); Anthocyanins (from bilberry fruit and grape skin extracts).

The invention relates to a medicine for treating Alzheimer's disease. The medicine is prepared from the following components in parts by weight: 68-138 parts of curcumin, 0.4-0.8 part of piperine, 45-90 parts of icariin, 26-52 parts of total ginsenoside; or 180-360 parts of fisetin, 0.4-0.8 part of piperine, 45-90 parts of icariin and 26-52 parts of total ginsenoside. The medicine utilizes the characteristics of multi-target regulation and control of traditional Chinese medicines, and has great potential when applied to AD prevention and treatment.

The invention relates to application of fisetin in preparation of a medicine for preventing and treating uric acid nephropathy, and belongs to the field of medicine. The invention provides application of fisetin or salt thereof in preparation of the medicine for treating and / or preventing the uric acid nephropathy. Animal experiments prove that the fisetin can regulate protein expression of kidney urate transporters URAT1, OAT1, OAT3 and ABCG2, improve uric acid excretion rate and obviously reduce serum creatinine, blood urea nitrogen and blood uric acid levels, also has an effect of inhibiting renal interstitial fibrosis, can obviously reduce expression quantities of fibrosis-related proteins such as alpha-SMA, cologen I and fibronectin, has an exact prevention / treatment effect on the uric acid nephropathy and provides a new choice for clinical medication.

The invention discloses a Chinese and Western medicine compound caring agent for pathological alopecia and a preparation method of the caring agent. The caring agent mainly consists of the following ingredients: a lombardy poplar extract, an extract of angled bittersweet root, an extract of densehead mountainash fruit, an extract of sinopodophyllum fruit, a russula sanguinaria extract, an extract of tricyrtis macropoda miq., an extract of trapa incisa var. sieb., an extract of glabrousleaf pittosporum root, an extract of Liangshan cordyceps herb, an extract of orchidaceae pecteilis, an extract of nepal fleshspike herb, an extract of urophyllous anotis herb, an extract of fortune paulownia bark, an extract of muskmelon leaves, an extract of trametes robiniphila murr, an extract of bunge bedstraw herb, an extract of radix actinidiae chinensis, propafenone, hesperetin, lycopene, beta-quebrachol, panthenol, an epidermal growth factor, fisetin and magnesium carbonate. The caring agent disclosed by the invention has functions of invigorating spleen and reinforcing stomach, dispelling wind and eliminating dampness, cooling blood and eliminating toxins, nourishing blood and tonifying deficiency, tonifying lung and kidney, promoting hair generation and moistening; the caring agent is capable of taking a conditioning effect from inside to outside, and the caring agent is capable of boosting immunity, relieving pressure and improving blood supply and oxygen supply to brain cells, so as to effectively prevent and treat the pathological alopecia; and the caring agent is good in clinical application effect.

The invention provides a synthesis method of fisetin, and solves the problems that the existing fisetin synthesis method is only applicable to laboratories, and the product yield and the content cannot be both obtained. The synthesis method comprises the following steps that 1,2-butanone, benzyl chloride, 2,4-dihydroxyacetophenone and anhydrous K2CO3 are used for generating an intermediate productwith protection groups; 2, under the nitrogen gas protection and in an alkaline environment, the intermediate product and protocatechualdehyde generate condensation reaction to generate 3',4'-dihydroxy-7-benzylchalcone; 3, the 3',4'-dihydroxy-7-benzylchalcone is reduced to generate 3',4',7-trihydroxychalcone; 4, the 3',4',7-trihydroxychalcone is put into the alkaline environment for hydroxy addition; then, cyclization reaction is performed under the para-toluenesulfonic acid catalysisto obtain the fisetin. The synthesis method can be used for industrial production; the synthesis method has the advantages that the operation is simple; raw materials can be easily obtained; the production cost is relatively low; the yield and the purity of the obtained intermediate product and the final product are relatively high; relatively good development prospects are realized.

The invention discloses an application of fisetin for resisting against Eimeria tenella. Fisetin is uniformly mixed with acceptable auxiliary materials, and the mixture can be added to water or feed for resisting against Eimeria tenella effectively.

The invention relates to a composition comprising fisetin as an active ingredient. The composition of the invention has an effect of improving or preventing skin aging, wrinkles, decreased elasticity, and decreased moisture content, and specifically, has the effect of fundamentally improving and preventing the wrinkles, aging, decreased elasticity, etc. of the skin by promoting the synthesis of collagen (type I) and sulfated glycosaminoglycans in the skin. In addition, since the composition is derived from a natural substance, the composition has low toxicity and few side effects and can be very effectively used as active ingredients in cosmetics, pharmaceuticals or foods, etc.

Nutraceutical formulations that improve cognitive function in adults diagnosed with neurodegenerative disease are provided, which comprise a combination of fisetin and DHA.

The NF-E2-related factor 2 (Nrf2) is a key transcriptional regulator of antioxidant defense and detoxification. To directly monitor stabilization of Nrf2 we fused its Neh2 domain, responsible for the interaction with its nucleocytoplasmic regulator, Keap1, to firefly luciferase (Neh2-luciferase). It is shown herein that Neh2 domain is sufficient for recognition, ubiquitination and proteasomal degradation of Neh2-luciferase fusion protein. The novel Neh2-luc reporter system allows direct monitoring of the adaptive response to redox stress and classification of drugs based on the time-course of reporter activation. The novel reporter was used to screen a library of compounds to identify activators of Nrf2. The most robust and yet non toxic Nrf2 activators found—nordihydroguaiaretic acid, fisetin, and gedunin-induced astrocyte-dependent neuroprotection from oxidative stress via an Nrf2-dependent mechanism.

The invention discloses fisetin with urate transporter 1 inhibitory activity and a preparation method and application thereof. The preparation method comprises the following steps: carrying out superfine grinding on licorice roots, and sieving to obtain superfine powder; soaking the superfine powder in ethanol, performing ultrasonic-assisted extraction, filtering and collecting filtrate, repeatedly extracting and filtering, and merging the filtrate to obtain a crude extract; concentrating the crude extract under reduced pressure to obtain an extract, redissolving the extract with water, extracting with an organic solvent, separating to obtain extract liquor, repeatedly extracting and combining the extract liquor; and carrying out macroporous resin coarse separation, chromatographic column fine grading and high performance liquid chromatography purification on the extract, and drying to obtain fisetin. The preparation method disclosed by the invention is simple and strong in specificity, the obtained fisetin has in-vitro urate transporter 1 inhibitory activity, and a new application of the glycyrrhiza uralensis fisetin is developed.

The invention discloses an iron casting surface antirust coating for body building. The thickness of the coating is 80-95 microns. The coating is formed by coating the following components in parts by weight: 20-40 parts of water soluble epoxy resin, 4-6 parts of pigments, 2-8 parts of silica sol, 4-15 parts of modified bone meal, 4-8 parts of nanometer negative ion powder, 0.5-3 parts of fisetin, 4-10 parts of fish oil, 1.5-5 parts of cyclohexanone and 4-8 parts of corn starch. According to the antirust coating disclosed by the invention, the modified bone meal is added into the traditional formula, so that the prepared coating is not cracked after being coated, does not blister and is uniform, high in surface quality, low in cost and small in injury of a human body.

The invention belongs to the field of traditional Chinese medicine preparations / health-care foods, and particularly relates to a fisetin phospholipid complex, a weight-reducing medicine / health-care food and an application thereof. The preparation method comprises the following steps: dissolving fisetin and phospholipid in an organic solvent, and reacting to prepare the fisetin-phospholipid complex; and the weight-reducing medicine / health food is prepared from 10-50% of fisetin and / or fisetin phospholipid complex and / or fisetin nanocrystals and / or fisetin cyclodextrin complex, 10-50% of a fructus lycii extract, 10-50% of a semen cassiae extract, 10-50% of a lalang grass rhizome extract and 10-50% of a radix astragali seu hedysari extract. According to the fisetin phospholipid complex and the weight-reducing medicine / health food provided by the invention, the water solubility of fisetin is improved by 10 times, the bioavailability is improved by 230%, and the fisetin phospholipid complex and the weight-reducing medicine / health-care food have a good weight-reducing effect, can relieve liver injury caused by metabolic stress while reducing weight, and are high in safety.

The invention discloses application of fisetin in resisting of Aeromonas hydrophila infection of aquaculture animals. The fisetin can lower in-vivo and in-vitro pathogenicity of Aeromonas hydrophila through directly inhibiting activity of aerolysin and is free of influence on growth of the Aeromonas hydrophila. Further, through constructing test animal models, it is discovered that the fisetin hasa remarkable therapeutic action on Aeromonas hydrophila artificial-infected models of Ictalurus punctatus, and the death rate in 8 days is lowered to 25%. The fisetin is an effective ingredient of aplant, i.e., smoke tree, meanwhile, is also distributed in vegetables and fruits such as onions and strawberries, is a flavonoid compound with relatively high safety and can be used for treating and preventing the Aeromonas hydrophila infection of the aquaculture animals as a drug or feed additive.

The invention discloses a method for preparing fisetin by an enzyme method. The method comprises the steps: crushing a raw material cotinus coggygria, and adding a citric acid solution and a plant extraction compound enzyme so as to enable the fisetin to be fully dissolved out of the citric acid solution; collecting enzymatic hydrolysate; concentrating the combined enzymatic hydrolysate into extract, adding high alcohol into the extract until the high alcohol is completely dissolved, and concentrating, crystallizing and drying filtrate to obtain a fisetin crude product; and recrystallizing the fisetin crude product twice by using a recrystallization solvent to obtain a high-purity fisetin refined product after twice recrystallization. According to the method, the citric acid solution is adopted to extract fisetin, so that fat-soluble substances in the enzymatic hydrolysate are extremely few, refining can be directly completed through a recrystallization process, a column chromatography process is not needed, the process operation difficulty and efficiency are reduced, the production efficiency is improved, and the method is suitable for industrial production. According to the invention, the recrystallization process is optimized, so that the refined fisetin product obtained after recrystallization is a needle-like crystal.

The invention discloses a plant extract composite buccal tablet, comprising the following raw materials: L-leucine, resveratrol, quercetin, anthocyanin, fisetin, pterostilbene, nicotinic acid, folic acid, chromium, magnesium, Coenzyme Q10, curcumin, lipoic acid, green tea extract, and milk thistle extract. According to the invention, the purely natural plant substances such as green tea extract and milk thistle extract added to the buccal tablet have significant therapeutic effect on respiratory diseases, digestive diseases, circulatory diseases, urinary system diseases, endocrine diseases, metabolic diseases, lymphatic diseases, neurological system diseases, connective tissue disease, gynecological diseases, otolaryngology diseases, stomatological diseases, orthopedic diseases, dermatological diseases, oncological diseases and other chronic degenerative diseases. Meanwhile, the buccal tablet is low in cost and free of side effects, has the efficacy of clearing heat and detoxifying, moving qi and activating blood, nourishing and strengthening body, regulating qi and invigorating stomach, benefiting qi and tonifying kidney, tranquilizing and tonifying spleen, and also is capable ofenhancing physical fitness to improve the immunity of the body to diseases.

The invention discloses compound fisetin microemulsion gel as well as a preparation method and application thereof. The invention provides compound fisetin microemulsion gel comprising fisetin, a compound fisetin microemulsion and a compound fisetin microemulsion gel matrix. The mass ratio of the fisetin to the compound fisetin microemulsion to the compound fisetin microemulsion gel matrix is (2.25-2.7): (50-60): (40-50). According to the invention, natural extracts of fisetin and litsea cubeba oil are used in a compatible manner and the compound fisetin microemulsion gel prepared by combiningthe microemulsion gel preparation technology can synergistically exert the biological activity of fisetin and litsea cubeba oil. The compound fisetin microemulsion gel is non-irritant to skin and mucous membranes and good in stability and has the remarkable effects of inhibiting skin oxidative damage and cell apoptosis and inhibiting melanin formation. Moreover, the preparation process of the microemulsion gel is simple, and the use is convenient. Thereofre, the fisetin microemulsion gel has the broad application prospects in preparation of skin care products with whitening, anti-aging and freckle-removing effects.

The invention provides a method of separating and extracting fisetin and myricetin in cotinus coggygria simultaneously. The method is simple in process route, comprises few steps and can separate andextract fisetin and myricetin simultaneously specifically and efficiently. The invention provides a pharmaceutical composition, a food additive composition and a cosmetics composition containing the cotinus coggygria extract prepared by the method.

The invention discloses a weight losing biscuit which comprises, by weight ratio, 100 parts of wheat flour, 18 parts of white granulated sugar, 3 parts of milk powder, 0.7 part of salt, 9 parts of dehydrated lotus leaf fragments, 11 parts of plant shortening, 1 part of baking soda, 8 parts of starch, 9 parts of eggs, 0.05 part of naringin dihydrochalcone, 0.04 part of artesunate, 0.07 part of astragaloside, 0.7 part of fisetin, 0.07 part of matrine and 0.1 part of asiatic acid. When the weight losing biscuit is prepared, the wheat flour and the starch are filtered; the auxiliary materials which are the plant shortening, the white granulated sugar, the salt, the dehydrated lotus leaf fragments, the eggs, naringin dihydrochalcone, artesunate, astragaloside and fisetin are mixed with the wheat flour and water to manufacture dough; when the dough is manufactured, after the baking soda is added or the wheat flour is fermented, the auxiliary materials are added to be mixed to manufacture the dough; and after the dough is manufactured, shaping and baking are performed. Preferably, ribs and stem are removed from the dehydrated lotus leaf fragments, and then the dehydrated lotus leaf fragments are cleaned and drained, dried at 75-90 DEG C, and smashed for standby application.

The invention discloses an application of fisetin and a salt thereof to preparation of drugs resisting radiation damages, relates to the technical field of medicines and particularly discloses the fisetin which can be applied to multiple radioactive organ damages and DNA damages caused by ionizing radiation, such as X-rays and gamma-rays, and simultaneously can be applied to adjuvant drugs for radiotherapy for tumors. The fisetin and the salt thereof disclosed by the invention widely exist in the nature and have significant prevention and treatment effects on multiple radiation damages. Therefore, a new idea is provided for the application of the fisetin; a new raw material is provided for the drugs resisting the radiation damages; and the fisetin is applied to the drugs resisting the radiation damages, so that the production cost of the drugs can be reduced, market promotion is facilitated and the economic pressure of patients is alleviated.