92 results about "Protective enzymes" patented technology

The invention relates to a method for improving activity of antioxidant enzyme of festuca arundinacea by using fine domestic garbage compost under drought conditions. In the method, a plastic pot with diameter of 7cm and height of 10cm is used through a pot experiment; and the method comprises the steps of: putting 150g of soil into each pot; sowing 0.5g of lawn plants in each pot; uniformly spreading 3g of municipal domestic garbage fine compost with particle sizes of 1,200nm, 600nm and 300nm on the surface of the soil; uniformly covering 20g of soil on the surface of the soil; controlling the temperature to be between 20 and 26 DEG C, wherein the relative humidity is between 40 and 60 percent, and illumination is natural light transmitting indoors; fully watering the plants in the initial stage of sowing, and watering by weighing every day to every two days according to 55 to 65 percent of water-holding capacity in fields after two weeks so as to maintain the capacity within a stress range; and measuring all the indexes on the 44th day. A result shows that the fine compost protects the festuca arundinacea plant from being damaged by active oxygen radicals, and reduces adverse environmental stresses to the plant caused by drought stresses.

The invention discloses a method for preparing regenerative silk fibroin. The method includes adding a calcium alcohol solution into degumming silks, dissolving the degumming silks through water bath, centrifugally removing suspended impurities, and performing dialysis and drying to obtain the regenerative silk fibroin. The preparation method is simple, the dissolvability of the silk fibroin is good, the structure of the prepared regenerative silk fibroin is a beta-folding sheet layer, the irregular curl structure is decreased, the regenerative silk fibroin can be used as a carrier for immobilizing enzyme, and the enzyme can be immobilized on the regenerative silk fibroin, so that the enzyme can be prevented from being degraded by degeneration enzymes in an organism, and further the stability and the half-life period of the enzyme are improved.

This invention relates to isolated or recombinant N-CBZ-deprotecting enzyme polypeptides that catalyze the removal of CBZ from CBZ-protected amino acids and alcohols, and variants, modifications, and fragments thereof. Also related are isolated nucleic acids encoding N-CBZ-deprotecting enzyme polypeptides, and variants, modifications, and fragments thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using polypeptides in various reactions for industrial or pharmaceutical applications.

The invention discloses application of a municipal domestic waste compost composite bacterial agent to improving drought resistance of lawn grass. The compost composite bacterial agent consists of liquid which is formed by diluting bacillus subtilis, actinomycetes and saccharomycetes in a volume ratio of 1 to 1 to 1 by 100 to 200 times. A result shows that drought damage of plants can be relieved to maintain a normal physiological metabolism function of the plants through activity of protective enzyme and osmotic adjustment substance content after diluted municipal domestic waste compost composite microbial bacterial agent is inoculated in the plants, so that the damage to the lawn plants caused by drought stress is effectively relieved, the drought resistance of the lawn plants is improved, and a basis is provided for planting of the lawn plants under a drought environment.

The invention relates to B. vallismortis HSB-2 and application thereof. The preservation number of the B. vallismortis HSB-2 is CGMCC No.15305, and the sequence of 16S rDNA is shown as SEQ.ID.NO.1. The invention further relates to the application of the B. vallismortis HSB-2 to preparation of bacterial fertilizer for relieving the successive cropping obstacle of apples. The B. vallismortis HSB-2 has an obvious antagonistic effect on four types of Fusarium fusarium pathogenic bacteria causing the successive cropping obstacle of the apples; the bacterial fertilizer manufactured through the B. vallismortis HSB-2 can promote growing of the overground parts and the underground parts of Malus malus hupehensis seedlings, and at the rhizosphere, colonization can be stabilized, and growing of the four types of Fusarium fusarium pathogenic bacteria of solani, proliferatum, verticillioides and oxysporum, causing the successive cropping obstacle of the apples, in soil can be remarkably restrained;and activity of soil urease, phosphatase, sucrase and root system protective enzymes, namely SOD, POD and CAT can be remarkably improved, and the successive cropping obstacle can be relieved.

The invention discloses application of remedied domestic waste composts in adjusting the activities of antioxidant enzymes of lawn plants; wherein, the remedied waste composts refer to compost matrixes obtained by eluting the composts with water based on the weight ratio of 1:1-3; the activities of the antioxidant enzymes comprise superoxide dismutase, peroxide dismutase and catalase. The technical effects of the invention show that the method of planting lawn plants on the remedied waste composts not only can enhance the activities of the protective enzyme systems of the lawn plants but also can improve the capabilities of the lawn plants in resisting unfavorable growth environment obviously, therefore, the technology not only finds a way for scientifically and effectively planting environment-friendly lawns on the matrixes of the eluted compost, but also can achieve the purpose of reducing the environment pollution caused by the domestic waste composts and provide technical support for applying the remedied waste composts to the lawn planting system.

The invention discloses a Bacillus velezensis strain NH-1 capable of preventing cucumber fusarium wilt. The Bacillus velezensis strain NH-1 is preserved at China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2017336. The invention also discloses a microorganism bacteria preparation capable of preventing cucumber fusarium wilt. The active ingredient of the microorganism bacteria preparation is the Bacillus velezensis strain NH-1. The microorganism bacteria preparation is high in preventing and controlling efficiency, is safe, is economical, and is friendly to theenvironment; effect lasts long; and cucumber fusarium wilt prevention is realized via increasing the activity of protective enzymes in cucumber.

The invention relates to a method for planting lawn plants by combining rare earth cerium with mixed culture matrices. The method comprises the step of sowing seeds of lawn plants on mixed culture matrices for lawn planting, wherein 1.5-2kg of mixed culture matrix soil contains 200-800mg / Kg of rare earth cerium, and the mixed culture matrices refer to soil and municipal solid waste compost in a ratio of parts by weight of 4: (1-2). By applying a certain amount of rare earth cerium, the activities of protective enzymes of the lawn plants can be obviously improved, and the defence capability of the protective enzyme system can be enhanced, thus improving the drought resistance of the lawn plants. Efficient and low-cost operation modes in large-area municipal lawn planting are expected to be achieved through the application of the technology.

The chelating state micronutrient fertilizer with the insecticidal function comprises trace elements and chelating agents and also comprises botanical pesticides and dispersion agents; the chelating state micronutrient fertilizer is shaped like powder; chelating agents are selected from tetrasodium iminodisuccinate salt and or EDTA (Ethylene Diamine Tetraacetic Acid); the botanical pesticides comprise Cynanchum komarovii Al. extracts and also comprise Stellera chamaejasme extracts and Ageratina adenophora extracts. According to the chelating state micronutrient fertilizer with the insecticidal function, the trace elements are added, trace elements and other nutrients brought by a plant source which serves as the pesticides are contained, the trace elements and the other nutrients can be chelated into the chelating state micronutrient fertilizer, the botanical pesticides are also contained, and accordingly the formula is reasonable, the synergistic effect is obvious, the utilization rate of effective components is high, the pest killing effect is good through due to the double insecticidal mechanism for damaging protective enzyme systems and nervous systems of nematodes and the fumigation action of multiple ingredients, and the botanical pesticides are free of pest resistance. The invention also relates to a production method of the chelating state micronutrient fertilizer with the insecticidal function.

A method for identifying modulators of the Keap1-NrG-ARE pathway is described. In particular, an assay is described that identifies molecules that inhibit the binding of a labeled Nrf2 peptide with the kelch domain of the Keap1 protein. Molecules that inhibit the binding are activators of the Keap 1-Nrf2-ARE pathway. Activation of the Keap 1-Nrf2-ARE pathway may result in an increased accumulation of Nrf2 and the subsequent induction of protective enzymes, for example, the phase 2 detoxification enzymes. Activators of the Keap1-NrG-ARE pathway are useful for combating oxidative stress-related disorders, such as those associated with cancer, emphysema, Huntington's disease, light-induced retinal damage, and stroke.

The invention discloses a maize breeding method. A BT insecticidal toxic protein gene, a bar gene and maize dwarf mosaic virus coat protein gene MDMVCPDNA are introduced into embryogenic maize calluses through particle bombardment, so that seeds with excellent insect resistance, weed resistance, disease resistance and drought tolerance are obtained; through seed soaking treatment by seed-soaking liquid, germination of seeds is promoted, the respiration rate of seedlings is increased, the root activity of the seedlings is improved, and the resistance of both the seeds and the seedlings is enhanced; through drip irrigation of a drought-resistant nutrient and by using anti-oxidation substances of phenolic acids, the number and activity of the protective enzymes in the plants are improved, the content of polyphenol substances is increased, the content of anti-oxidation substances in the crop is increased and the drought resistance of the crop is enhanced; meanwhile, since the content of the anti-oxidation substances in the crop is increased, the disease resistance, UV radiation resistance and the like of the crop are improved; since monopotassium phosphate and nitrogen are added into the phenol substances, nutrient elements supplied to the crop are increased.

The present invention is directed to restricted access media (RAM), methods for preparing restricted access media, and kits for preparing restricted access media that contain protected ligand binding agents or protected enzymes. Certain RAM provided contain a plurality of protected regions of the support that contain ligand binding agents that are protected by blocking agents. Certain RAM provided contain a plurality of protected regions of the support that contain unbound ligand binding agents or enzymes that are retained in the protected regions by a capping agent. Methods of making the RAM of the invention and associated kits are also provided

The invention discloses a preparation method of nutrient-balanced fertilizer special for tea trees. The preparation method comprises the following steps that peanut meal, humic acid, corncob powder, coconut bran, castor meal and water are mixed and fermented to obtain a prefabricated material; the prefabricated material and a modified dispersant are mixed uniformly, ammonium nitrate, triple superphosphate, kiln dust potash fertilizer, zeolite powder, sepiolite powder, EDTA chelate zinc, humic acid copper and EDTA chelate iron are mixed uniformly, and granulating, sieving and drying are conducted to obtain the nutrient-balanced fertilizer special for the tea trees. Not only is the fertilizer special for the tea trees extremely good in nutrient substance slow-release effect, the demands of the tea trees during the whole growth period are met simply by applying the fertilizer once, but also harmful bacteria can be effectively inhibited, the soil fertility is enhanced, the growth capability of the tea trees is stimulated, the protective enzyme activity of tea leaves can be effectively improved, the disease resistance of the tea trees is improved, at the same time, the crumb structure of soil can be reformed, the soil is looser, and the fertilizer is conducive to the growth of the tea trees.

The invention discloses an application of eutrophic water in the regulation of antioxidase activity of lawn plants, wherein the eutrophic water is the eutrophic water with 25 percent-75 percent of the original concentration; and the eutrophic water has TN of 9.7 mg / L-13.1mg / L, TP of 0.6mg / L -0.9mg / L and pH value of 8.12-8.99, wherein the activity of antioxidase comprises of superoxide dismutase, peroxide dismutase and catalase. Results show that the diluted eutrophic water is applied to irrigation to reinforce the activity of a protective enzyme system of lawn plants, thereby being capable of obviously improving the resistance ability of the lawn plants to adverse growing environment and providing technical support for the application of the eutrophic water in a lawn plant establishment system.

The invention discloses a method for improving winter resistance of turf by adopting household garbage compost offscourings. The ryegrass seedlings cultivated by compost and cold-hardened in cold environment with gradually decreased temperature have no obvious difference from the seedlings in the control group in indexes such as protective enzyme, proline and conductivity, but are slightly shorter. The aboveground biomass of the cold-hardened ryegrass seedlings cultivated by compost offscourings is reduced by 26.74% compared with that of the seedlings in the control group and is reduced by 23.21% compared with that of the seedlings with relatively poorer matix nutrition in the filter paper group. The final technical effect shows that the cold-hardened turf can fully resist winter in the winter environment of not less than 10 DEG C below zero in the outdoor balcony without being provided with a heating facility, and the aim of storing the turf in winter and producing all year around is achieved.

The invention discloses a method for improving the salt resistance of tall fescue by virtue of an air-dried sludge microbial agent. The microbial agents comprise Pseudomonas stutzeri + Trichoderma reesei + 100-fold dilution of Streptomyces flavus or Pseudomonas stutzeri + Trichoderma reesei + 200-fold dilution of Streptomyces flavus. The experimental results show that the plant height, biomass and chlorophyll content of tall fescue can be significantly improved, the contents of malonaldehyde and proline are lowered and the protective enzyme activity of tall fescue is enhanced by applying sludge complex microbial agents. On the whole, the microbial agents exhibit better effects in low fertility and the salinized soil, which fully shows the potential applications of microbial agents in the saline-alkali soil improvement.

The invention relates to a preparation method of a ZIF-8 / enzyme composite material. The preparation method comprises the steps of regulating the concentration of a precursor to enable a ZIF-8 crystalto grow on the surface of an enzyme in a lamellar structure, mixing a metal salt water solution with a mixed solution of a ligand water solution and the enzyme, performing stirring at room temperaturefor a period of time, and separating out a solid product, and thereby obtaining the ZIF-8 / enzyme composite material. Different from a composite material obtained through a traditional method, the method does not depend on ducts of ZIF-8 to diffuse substrate molecules, and a large-size substrate makes contact with the active center of the enzyme through gaps between ZIF-8 lamellar structures, so that a catalytic reaction is completed. ZIF-8 nanoflowers growing on the surface of the enzyme can well protect the enzyme from reacting under harsh conditions, and meanwhile, the ZIF-8 / enzyme nanoflower composite material also has good circularity and storage capacity.

The invention relates to a preparation method and application of total nutrient microorganism biocontrol organic fertilizer. The preparation method of the total nutrient microorganism biocontrol organic fertilizer comprises the following steps: firstly, carrying out plate cultivation on trichoderma pseudokoningii TRIPI and rhizopus nigricans RN-9; secondly, carrying out PDB liquid culture on TRIPI and RN-9 hypha blocks; thirdly, carrying out fermentation culture on a liquid culture solution, so that fermentation liquor is obtained; fourthly, inoculating the fermentation liquor to farmyard manure raw materials, uniformly mixing, stockpiling and fermenting, so that a fermented material is obtained; fifthly, adding other ingredients into the fermented material, and uniformly mixing, so that the total nutrient microorganism biocontrol organic fertilizer is obtained. The prepared total nutrient microorganism biocontrol organic fertilizer has high inhibition ratio on pathogenic bacteria and high relative control efficiency on fusarium wilt of cucumber; compared with biocontrol organic fertilizer prepared by adopting single strain fermentation liquor, content of pathogenic bacteria in cucumber rhizosphere soil, cucumber root system and cucumber stalk is low, and cucumber blade protective enzyme is quick in response, high in activity and long in duration of time.

The invention discloses a method for employing lawn nursery strip to adjust Festuca arundinacea water-stress protective enzyme. Waste newspaper and waste cotton cloths are used as a lower layer of a lawn nursery strip planting carrier; waste gauzes and waste face tissues are used as an upper layer of the lawn nursery strip planting carrier; a potting method is adopted for adding dried original soil in a container; then the lawn nursery strip is paved on the original soil flatly; and water required for seed germination is guaranteed after sowing until the seeds are germinated; then stress is started after the germination occurs for 5d and the water content in the soil is maintained at 35-50 percent of saturated water-holding capacity; after the germination occurs for 40d, various physiological and biochemical indexes of Festuca arundinacea seedlings are measured and the overground parts of the seedlings are cut; then the Festuca arundinacea seedlings are continuously cultured for 40d and then eddish and the underground parts are harvested; the total cultivation period lasts for 80d; the average indoor temperature is 18-22 DEG C; and the average humidity is 30-40 percent. Test result shows that: the lawn nursery strips of wastes can play a better role of regulating the growth of lawn grasses during the water-stress period of the plants.

The invention provides a method for relieving drought stress of Actinidia chinensis fruit trees. The method comprises the following step of irrigating melatonin at the concentration of 50 to 200 umol / L in seedlings of the Actinidia chinensis for one time every other 2 days, totally for four times, wherein the irrigation amount is 200 ml. By the method, the relative water content of leaves of the seedlings of wild Actinidia chinensis in the same year under the drought stress can be obviously increased, damage of malonaldehyde to cytomembrane is reduced, the relative membrane permeability is reduced, proline concentration and soluble sugar concentration are reduced, degradation of soluble protein is inhibited, high activity of protective enzyme is maintained, the plants can adapt to the drought environment better and the drought resistance of the plants is enhanced.

The present invention provides for methods of administering by a pulmonary route an effective amount of a bisphosphonate active agent to a subject. Aspects of the invention including administering the active agent to the subject in conjunction with one or more mucosal membrane protecting agents, where the protecting agent may include one or more of a protecting enzyme and / or a protecting amino acid and / or a protecting peptide. Also provided are inhalant compositions for use in practicing methods according to embodiments of the invention. Methods and compositions according to embodiments of the invention find use in a variety of different applications, including but not limited to, the treatment of bone adsorption disease conditions.

The invention discloses a method for improving protective enzyme activity of turfgrass plants by utilizing air-dried sludge. Air-dried sludge combination substrates comprise sludge and soil with, by weight, the part ratio of 1: (3-7). Experiments show that air-dried sludge can facilitate the growth of the turfgrass plants evidently. When festuca arundinacea and lolium perenne are planted in conditions of soil and sludge combination substrates with the part ratios of 1:7, 3:13 and 1:3 respectively, the activities of turfgrass protective enzymes of POD (peroxidase) and CAT (catalase) are improved evidently and show significant differences with control groups. Therefore, the dewatered sludge of a sewage treatment plant can be directly applied to the cultivation of the turfgrass plants after being air-dried naturally to improve the activities of the turfgrass protective enzymes activity.

The invention discloses a method for improving protective enzyme activities of salt-stressed turfgrass by adopting an enhanced salt-tolerant microbial agent. The enhanced salt-tolerant microbial agent comprises enhanced bacillus subtilis, enhanced lysine bacillus and enhanced penicillium chrysogenum in a weight part ratio of 1 to 1 to 1, wherein enhanced bacillus subtilis, enhanced lysine bacillus and enhanced penicillium chrysogenum are salt-tolerant mixed microbial flora obtained by carrying out domestication step by step with a method for increasing the salt concentration step by step. The invention further discloses an application of the enhanced salt-tolerant microbial agent in improvement of the protective enzyme activities of salt-stressed turfgrass festuca arundinacea L. Experimental results show that the compound enhanced microbial agent can conduce to effectively alleviating the harms of saline-alkali soil to the turfgrass; screening and culturing corresponding enhanced salt-tolerant compost microbial strains and carrying out artificial inoculation is a new way for improving the salt tolerance of the turfgrass.

The invention discloses a method for improving festuca arundinacea drought resistance by adopting a dried sludge fungicide. The raw material used in the method is a 100-fold diluent of pseudomonas stutzeri, trichoderma reesei and yellow streptomycete or a 200-fold diluent of pseudomonas stutzeri, trichoderma reesei and yellow streptomycete. The research result shows that the complex microbial agent can form a good symbiotic relationship with a lawn plant; the complex microbial agent can enhance the drought resistance of the plant and promote plant growth by direct action of promoting absorption of host plant roots on the soil moisture and by indirect action of improving physiological and metabolic activity inside a plant body and improving the protective enzyme activity, and the water-saving percentage of the 200-fold diluent of the pseudomonas stutzeri, the trichoderma reesei and the yellow streptomycete can be up to 57.39%.

The invention provides a method for preparing germinated brown rice by utilizing a repeated mild drought stress method. The method is characterized by placing the brown rice into drought stress solution to compel the soluble sugar, free amino acids and active oxygen to be accumulated, activating protective enzymes and phenylalanine ammonia lyase and promoting the amount and activities of superoxide dismutase, hydrogen peroxidase and peroxidase to be increased and polyphenol compounds to be enriched. The method has the following advantages: the method of alternately carrying out repeated and short drought stress and high temperature germination hastening is adopted to prevent excessive active oxygen from harming the cells, thus ensuring the active oxygen to be slowly generated and not only obtaining higher contents of soluble sugars, free amino acids, superoxide dismutase, hydrogen peroxidase, peroxidase and polyphenol compounds but also improving the germination percentage of the brown rice.

The invention discloses a seed breeding method for improving drought resistance of summer squashes, and belongs to the technical field of agricultural planting. The seed breeding method for improving the drought resistance of the summer squashes is provided for solving the problem that due to the fact that the summer squashes are under drought stress, the yield and quality of the seeds are seriously affected. Accordingly, an illumination and audio frequency physical method and a chemical method are organically combined, protective enzyme systems inside the summer squashes are effectively promoted and improved, characteristics of the drought resistance of the summer squashes are improved, and therefore the seed yield of the summer squashes is increased, and the seed quality is improved.

The invention relates to a method for aridity of ryegrass by using rare earth lanthanum and particularly relates to application to increasing activity of protective enzyme of ryegrass and stress resistance of ryegrass by using the rare earth lanthanum. The method comprises the following steps of: sowing seeds of ryegrass on garden soil subjected to lawn establishment, wherein every 100-200g of garden soil contains 200mg / Kg-800mg / Kg of lanthanum nitrate; and at the initial stage of sowing, performing watering thoroughly so as to guarantee the smooth germination of the seeds and the initial-stage growth of young seedlings. When certain amount of rare earth is used, the activity of the protective enzyme of the ryegrass is remarkably increased, the defense capability of a protective enzyme system is strengthened, so that the drought resistance of the lawn plants is improved; in addition, the application of the technology is hopeful for the realization of the efficient and low-cost operation mode for the large-area city lawn establishment.

The invention discloses a method for improving protective enzyme activity of grass of a water stress lawn by adopting a reinforced drought-resistant microbial agent. The reinforced drought-resistant microbial agent comprises the following components of reinforced bacillus cereuses, reinforced lysine bacillus and reinforced rhodotorula glutinis, with the weight ratio of 1: 1: 1. The drought resistance and the reinforcement of a mixed microbial population are performed by adopting the method of gradually increasing the concentration of PEG6000. The invention further discloses the application of the reinforced drought-resistant microbial agent in the field of improving the protective enzyme activity of festuca arundinacea of the lawn grass under the drought stress. Experiments prove that under the drought stress, the protective enzyme activity of the festuca arundinacea can be obviously improved, so that damage caused by the drought stress to the festuca arundinacea is alleviated, and the improvement of the drought-resistance of the lawn grass is facilitated.

The invention provides a method for promoting seed germination through regulating and controlling the content of zinc in soaking liquid and relates to a seed treatment method. The method is implemented by the following steps: a, soaking crop seeds with disinfection fluid with a volume concentration of 5-15 percent for 20-50 minutes and thoroughly washing with deionized water to obtain a disinfected crop seeds; b, soaking the disinfected crop seeds obtained in the step a with chelated zinc of 0.1-3 mmol.L<-1> for 16-40 h, so as to complete seed treatment. According to the method for promoting the seed germination through regulating and controlling the content of zinc in the soaking liquid, provided by the invention, the absorption and the utilization of zinc of a plant are facilitated, and meanwhile, antagonistic action among elements can also be avoided; through regulating and controlling the content of zinc in the soaking liquid, the promoting effect of zinc on the seed germination is judged from the perspective of seed germination, growth, and changes in protective enzyme activity. Through the seed soaking treatment with zinc, the situation that the plant growth is affected due to soil zinc deficiency is improved while the soil pollution caused by excessive application of zinc fertilizers in a farmland is avoided.