33 results about "Protein filament" patented technology

The invention relates to a protein viscose fiber and a preparation method thereof, belonging to the textile field and aiming at solving a technical problem about how to provide a method for adopting different animal and plant proteins as materials to prepare the protein viscose fiber. The preparation method of the protein viscose fiber includes the following steps: 1. the animal and plant proteins are degreased, decolored and deodorized to obtain animal-plant protein powder; 2. alkalization: the animal-plant protein powder is dissolved in alkaline liquor to obtain protein alkaline solution; 3. cross-linker is added into the protein alkaline solution to obtain a protein spinning liquor; 4. the protein spinning liquor is blended with a glue to be spun to obtainprotein filaments; 5. the protein filaments are washed by peroxide solution and are oiled, dried and formed into cylinder form to get the protein viscose fiber. The protein viscose fiber preparation method of the invention does not adopt any acetalation treatments to the protein filaments after spinning so as to avoid the remaining of methanal, etc. harmful matters, the technique steps are simple and can be easily operated and the manufacturing can be realized with the existing viscose fiber preparation equipment.

Methods for identifying and characterizing inhibitors of protein filament formation are provided. These methods are particularly useful for identifying those agents which inhibit or prevent protein filament formation within neurons of mammalian subjects, particularly human subjects, such as the formation of tau filaments in Alzheimer's patients, and α-synuclein filaments in Parkinson's patients. According to the methods, protein monomers which are associated with formation of intra- or extra-cellular aggregates are combined under physiological conditions with a fibrillization inducer and the formation of protein aggregates is assessed in the absence and the presence of a test agent. The absence or a reduction in the size or stability of proteinaceous polymeric filaments as compared to a control indicates that the test agent is an inhibitor of proteinaceous polymeric filament formation.

The invention relates to a colored protein viscose fiber and a preparation method thereof, belonging to the textile field and aiming at providing an equal-coloring colored protein viscose fiber preparation method. The preparation method of the invention includes the following steps: 1. animal and plant proteins are degreased, decolored and deodorized to obtain animal-plant protein powder; 2. alkalization: the animal-plant protein powder is dissolved in alkaline liquor to obtain protein alkaline solution; 3. cross-linker is added into the protein alkaline solution to obtain protein spinning liquor; 4. the protein spinning liquor is blended with a coloring glue to be spun to obtain protein filaments. 5. the protein filaments are washed by peroxide solution and are oiled, dried and formed into cylinder form to obtain the colored protein viscose fiber. The colored protein viscose fiber of the invention has equal-coloring process, high color fastness and color brightness, and the preparation method does not adopt any acetalation treatments to the protein filaments after spinning so as to avoid the remaining of methanal, etc. harmful matters, the technique steps are simple and can be easily operated and the manufacturing can be realized with the existing viscose fiber preparation equipment.

The invention discloses a manufacturing process of false eyelashes. The manufacturing process is characterized by comprising steps as follows: 1), a work bench is manufactured, and a plurality of scales are marked on the work bench; 2), human hair with uniform lengths or high-grade protein filaments with uniform lengths is or are taken as a raw material; 3), the number of bonding tapes arranged on the work bench is determined according to the length of the raw material, and the raw material is kept in a straightening state; 4), the straightened raw material is sheared by a false eyelash shearing tool, and finally, wastes are bundled; 5), the primarily sheared raw material is secondarily sheared and layered, and semi-finished false eyelashes are manufactured; and 6), the layered semi-finished false eyelashes are permed and curled, and the finished false eyelashes are manufactured. The manufacturing process is simple, a large quantity of raw materials is saved, the raw materials are utilized to the largest extent, the length of the raw material is not strictly restricted, and meanwhile, the labor efficiency is improved.

The invention discloses a method for preparing silk feather protein powder for preparation of protein filament, and relates to the technical field of cornifin protein preparation. The method comprises the following steps of preparation of acid leaching feather meal; preparation of feather meal alkaline solution; preparation of feather protein mixed liquor; preparation of feather protein precipitation liquid; and obtaining of the silk feather protein powder. Compared with the existing method, the protein content of the feather protein for silk drawing prepared by the method achieves above 95%, and the average molecular weight of the feather protein is 10000Da; and the protein solution for preparing adhesive protein filament has the characteristics of not generating peptone, being uniform in drawing silk, not breaking the silk and the like of the alkaline solution of high concentration, and is excellent feather protein powder for preparing protein filament.

The present invention relates to a preparation method of high-dietary fiber drawing protein. The drawing protein consists of the following raw materials: soy protein isolate, soy protein concentrate,low-temperature defatted soybean meal, wheat starch, wheat gluten, fresh bean dregs, and water. The preparation method comprises the following steps: (1) mixing the fresh bean dregs with the water ina ratio of 1:4, and conducting crushing in a colloid mill and high-pressure homogenization to obtain a bean dreg suspension; (2) uniformly mixing the soy protein isolate, the soy protein concentrate,the low-temperature defatted soybean meal, the wheat starch and the wheat gluten; (3) feeding the uniformly mixed material in a fixed amount into a twin-screw extruder, simultaneously injecting the homogenized fresh bean dreg suspension in a fixed amount, and carrying out extrusion and section-cutting forming; (4) conveying the shaped material into a drying box for drying; (5) cooling the finishedproduct in a temporary storage box; and (6) subjecting the product to fixed-amount packaging and warehousing. The method has the advantages that the drawing protein produced by the method has good forming effect, and the protein filament is good in elasticity and toughness after rehydration; and meanwhile, the dietary fiber content is high, and the nutritional value is high.

The invention discloses a skin health care towel which is characterized in that weft yarns and bottom yarns of the towel are pure cotton yarns, and the surface yarns of the towel are protein filament single yarns. A manufacturing method of the skin health care towel comprises processes of yarn raveling, thermal washing, sizing, beaming, roundabout, weaving, desizing oxygen bleaching and dyeing, sewing, detection and packaging. The manufacturing method is characterized in that in the sizing process, the dosage of components of sizing agents is that the weight of the protein filament yarns are 200 kg, the weight of corn starch is 2-4% of the weight of the protein filament yarns, and the weight of beef tallow and the weight of talcum powder are 0.3-0.5% of the weight of the protein filament yarns. In the desizing oxygen bleaching and dyeing process, bio-enzyme alkali-free oxygen bleaching desizing is used, the dosage of components of oxygen bleaching desizing liquid is that the weight of towel blank is 200 kg, the weight of copper clad laminate (CCL) is 0.1-0.2% of the weight of the towel blank, the weight of urea is 1-2% of the weight of the towel blank, the weight of hydrogen peroxide is 2-4% of the weight of the towel blank, and the weight of the brightening agents is 0.1-0.2% of the weight of the towel blank. The skin health care towel and the manufacturing method thereof have the advantages that the towel is bright in color, soft, comfortable, cheap and beautiful, the towel has health care effect on skin, the quality of the towel meets the national standard, bio-enzyme alkali-free oxygen bleaching desizing is used for replacing caustic soda processing technology, waste water has no alkali and is easy to treat, environment pollution is small, energy consumption is low, and the production process is clean.

The invention relates to a preparation method for manufacturing silkworm pupa protein of protein filaments, relating to a method for extraction of silkworm pupa protein from silkworm pupa. The defatted silkworm pupa available commercially is used as a raw material, and the product is obtained via preparation of alkaline solution of defatted silkworm pupa powder, chitin-free silkworm pupa protein solution and precipitation solution thereof and silkworm pupa protein powder for filaments and recycling of silkworm pupa protein peptide. The method is simple, the production equipment is conventional general equipment, the energy consumption is low, the cost is low, and the promotion and use can be facilitated; and high-quality silkworm pupa protein, silkworm pupa protein peptide and other products can be produced according to the method disclosed by the invention, the silkworm pupa can be comprehensively and fully utilized, no ''three-waste'' emissions can be produced, the environmental protection can be facilitated, and the method belongs to a preparation method of green bio-chemical products. The method can be widely applied in preparation of silkworm pupa protein and silkworm pupa protein peptide. The silkworm pupa protein prepared in the method can be applied in manufacturing of adhesive protein filaments, food additives, bio-chemical culture mediums for fermentation, feed additives and the like; and the prepared silkworm pupa protein peptide can also be used as a high-quality nutritional agent for animal cubs.

The invention relates to an implant material with a PRF (Platelet-Rich Fibrin) precursor liquid solidified into a gel mask, and belongs to the field of the implant materials for mucocutaneous operations. The implant material provided by the invention is characterized in that a non-anti-coagulation blood sample is employed, and centrifuged by a centrifuge with centrifugal force ranging from 130 to 460g, the upper total yellow liquid is put into standing at the room temperature for 30-40 minutes, and then the total yellow liquid is pressed into a mask for clinical application by a metal pressing plate. The implant material provided by the invention is high in yield, and can be naturally formed into gel without adding thrombin and calcium ions; as a result, the use risk of biochemical preparations is avoided; the operation of the implant material is simple; the PRF gel product is thick in fibrous protein filaments, good in elasticity and tenacity, strong in tension, high in hardness, slow in degradation, and not easy to break by tearing; almost no damage is caused in the preparation process of hemameba, blood platelet and shed particles of the two; and after being implanted in vivo, the implant material slowly releases various growth factors and anti-inflammatory cytokines to promote wound healing.

Methods for identifying and characterizing inhibitors of protein filament formation are provided. These methods are particularly useful for identifying those agents which inhibit or prevent protein filament formation within neurons of mammalian subjects, particularly human subjects, such as the formation of tau filaments in Alzheimer's patients, and α-synuclein filaments in Parkinson's patients. According to the methods, protein monomers which are associated with formation of intra- or extra-cellular aggregates are combined under physiological conditions with a fibrillization inducer and the formation of protein aggregates is assessed in the absence and the presence of a test agent. The absence or a reduction in the size or stability of proteinaceous polymeric filaments as compared to a control indicates that the test agent is an inhibitor of proteinaceous polymeric filament formation.

The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits.

The invention discloses a biological dressing for removing scars. The biological dressing is prepared from glucan, sodium pyrrolidone carboxylate, protein filament peptide, dunaliella salina, allicin,tomato stem leaf extract, pudilan extract, nanometer pearl powder, glucose oxidase, cinnamyl aldehyde, glyceric stearic acid, sodium lactate, choline chloride, glycollic acid, methyl trehalose fumarate and medical purified water. The invention also discloses a preparation method for the biological dressing for removing scars. The biological dressing for removing scars disclosed by the invention has low irritation, no toxic side effect and high biocompatibility; the biological dressing is capable of effectively restraining deposition of pigments on scars, boosting skin absorption and repairing, reducing inflammation and boosting cell metabolism, so as to achieve the purposes of removing scars and repairing skin wounds and keep skin complete and smooth.

The invention discloses a silk fibroin fiber hollow nerve guide and its preparation method and application, belonging to the technical field of biomedical materials. Using natural silk fibroin as raw material, it has good biocompatibility and biodegradability. The hollow nerve guide prepared by swelling the silk fibroin fiber with polydioxanone / formic acid solution has good compression resilience. The raw material price is low, the sources are wide, the operation method is simple and the process flow is short, the hollow nerve guide with different size requirements can be prepared quickly and conveniently, and it can be mass-produced. The prepared silk fibroin fiber hollow nerve guide has very good mechanical properties and compression resilience, is not easy to cause the collapse of the support material, and can ensure the normal growth of nerve cells. The scaffold material has high porosity and many connected pores, and nerve cells can adhere, proliferate, migrate and grow on the nerve conduit material, which can promote the regeneration and repair of damaged nerves.

The invention relates to a polyclonal antibody for efficiently recognizing proteinserine heptanose glycosylation, and a reparation method and application thereof. The antibody is used for detecting different structures of natural protein modified by proteinserine heptanose glycosylation and screening and discovering novel antibiotics. The preparation process of the antibody comprises the steps offirstly building a C7 sugar framework by Wittig reaction; then, obtaining six-bit heptose of two absolute structures through Sharpless asymmetrical oxidization reaction; under the help of glucosinolates and trichloroacetimidate mediated glycosylation reaction, obtaining proteinserine heptanose with the same glycosylation modification structure in the organism; then, obtaining the corresponding glycopeptide with the sequence being Ac-GS(Hep)GL-OH by using a polypeptide solid phase synthesis method; coupling the obtained semi-antigen onto the carrier protein BSA to obtain antigen; immunizing animals by the antigen; collecting immunized New Zealand rabbit blood to prepare antiserum; obtaining IgG through affinity purification. The prepared antibody has high specificity and high valence.

The invention provides a method for filtering educts after a sample in a procoagulant type test tube is subjected to a centrifugal action. The technical scheme is realized by comprising the following steps of: a. collecting blood into the procoagulant type test tube by using a vacuum blood taking technology, wherein the procoagulant type test tube is internally provided with a filter screen; and b. enabling the procoagulant type test tube to be subjected to the centrifugal action so that a solution in the procoagulant type test tube forms a zone belt with different levels, wherein a blood corpuscle layer and a blood serum layer are positioned in the procoagulant type test tube from bottom to top, and the filter screen sinks on the blood corpuscle layer along a tube wall under the action of centrifugal force. The invention can effectively filter out floating fiber protein filaments and separation gel educts in the blood serum obtained after centrifugation, reduces the pollution of the fiber protein filaments and the separation gel educts on a sampling probe and a colorimetric cuvette, and ensures the detection result of a blood sample to be more accurate.

The invention relates to a colored protein viscose fiber and a preparation method thereof, belonging to the textile field and aiming at providing an equal-coloring colored protein viscose fiber preparation method. The preparation method of the invention includes the following steps: 1. animal and plant proteins are degreased, decolored and deodorized to obtain animal-plant protein powder; 2. alkalization: the animal-plant protein powder is dissolved in alkaline liquor to obtain protein alkaline solution; 3. cross-linker is added into the protein alkaline solution to obtain protein spinning liquor; 4. the protein spinning liquor is blended with a coloring glue to be spun to obtain protein filaments. 5. the protein filaments are washed by peroxide solution and are oiled, dried and formed into cylinder form to obtain the colored protein viscose fiber. The colored protein viscose fiber of the invention has equal-coloring process, high color fastness and color brightness, and the preparation method does not adopt any acetalation treatments to the protein filaments after spinning so as to avoid the remaining of methanal, etc. harmful matters, the technique steps are simple and can be easily operated and the manufacturing can be realized with the existing viscose fiber preparation equipment.

The invention discloses an application of a composition in preparation of a skin barrier gene repair preparation. The composition includes the following components: a scutellaria baicalensis root extract, a sophora flavescens root extract, and a glycyrrhiza inflata root extract; and the application includes the aspect of using the composition to regulate expression of at least one protein or enzyme selected from the group consisting of keratin, filaggrin (FLG), loricrin (LOR), involucrin, transglutaminase, aquaporin, caspase-14 (CASP14). The preparation fills the blank of the composition of the three extracts of the scutellaria baicalensis, the sophora flavescens and the glycyrrhiza inflata in the repair of genes related to skin barrier in the prior art, and especially, the preparation, genes related to formation of cornified envelope (CE), such as the LORE and the FLG and a gene related to filaggrin degradation, such as the CASP14 lay the foundation for subsequent treatment of skin diseases related to the skin barrier.

The invention discloses shredded beef containing soybean protein filaments and a making method of the shredded beef. The shredded beef is prepared from raw materials as follows: large chunks of beef,water, soyabean protein columns, beef tallow, soybean oil, chilli powder, powder of pericarpium zanthoxyli, sesame, spice powder, glucose, edible salt, white sugar, garlic powder, onion powder, beef powder, chicken powder, cattle cream, yeast extract, rock candy water, monosodium glutamate, disodium 5'-ribonucleotide and fresh powder. After the soybean protein filaments are added on the original basis, the product has better oil absorbing and retaining effects, tastes tougher and more elastic during chewing and is more chewy, besides, the soybean protein filaments have a better taste adsorbingeffect, the product tastes like the shredded beef and can be more tasty with chewing, meat protein in the whole product is reduced, vegetable protein of the whole product is increased, the product meets the health concept, the cost can be reduced, the price on the market is far lower than that of pure shredded beef, so that the purchasing pressure of consumers is reduced, and the product has higher market value.

The invention relates to a preparation method for manufacturing silkworm pupa protein of protein filaments, relating to a method for extraction of silkworm pupa protein from silkworm pupa. The defatted silkworm pupa available commercially is used as a raw material, and the product is obtained via preparation of alkaline solution of defatted silkworm pupa powder, chitin-free silkworm pupa protein solution and precipitation solution thereof and silkworm pupa protein powder for filaments and recycling of silkworm pupa protein peptide. The method is simple, the production equipment is conventional general equipment, the energy consumption is low, the cost is low, and the promotion and use can be facilitated; and high-quality silkworm pupa protein, silkworm pupa protein peptide and other products can be produced according to the method disclosed by the invention, the silkworm pupa can be comprehensively and fully utilized, no ''three-waste'' emissions can be produced, the environmental protection can be facilitated, and the method belongs to a preparation method of green bio-chemical products. The method can be widely applied in preparation of silkworm pupa protein and silkworm pupa protein peptide. The silkworm pupa protein prepared in the method can be applied in manufacturing of adhesive protein filaments, food additives, bio-chemical culture mediums for fermentation, feed additives and the like; and the prepared silkworm pupa protein peptide can also be used as a high-quality nutritional agent for animal cubs.

Embodiments concern methods and small molecule compositions for selectively inhibiting RAD51-mediated D-loop formation while preserving RAD51's ability to form nucleoprotein filaments. The selective RAD51 D-loop formation activity inhibitors DNA repair while minimizing replication-associated toxicity in normal tissue.

The invention belongs to the technical fields of extraction of natural protein components and virology, and discloses a sericin protein, an extraction method, and its application as a cell antiviral immune enhancer. Uses; the dose concentration of sericin is 10-20mg / mL, and the effective final concentration is ≥1mg / mL; the extraction method of sericin includes: selecting available silkworm cocoons and storage conditions, silkworm cocoon pretreatment and sterilization, sericin dissolution, Dialysis and renaturation of sericin protein, concentration of sericin solution, removal of endotoxin in sericin solution, filtration of sericin solution, quality control inspection of sericin solution, sericin solution that meets the testing standards as a cell antiviral immune enhancer. The present invention develops and utilizes silk protein sericin for the first time, as the application of preventing and controlling virus infection. The raw material source of the invention is wide, and compared with the high synthesis and production costs of other antiviral drugs, the cost of the invention is low.

The invention discloses a protein sericin gel based on transformation of human platelet derived growth factor (PDGF-BB) gene silk and a preparation method and application of the protein sericin gel. By using the functional silk of the human-derived PDGF-BB, sericin with the active PDGF-BB protein is extracted, a gelling reaction of the sericin is induced at the low temperature, the prepared protein sericin gel is good in mechanical property, degradable and good in cell compatibility and does not have toxicity, the PDGF-BB can stably exist, apoptosis cannot be caused, the obvious cellular inflammatory response cannot be caused, and the sericin gel biological material with a function of improving the cell proliferation activity can be applied to the tissue engineering.

The invention relates to an implant material with a PRF (Platelet-Rich Fibrin) precursor liquid solidified into a gel mask, and belongs to the field of the implant materials for mucocutaneous operations. The implant material provided by the invention is characterized in that a non-anti-coagulation blood sample is employed, and centrifuged by a centrifuge with centrifugal force ranging from 130 to 460g, the upper total yellow liquid is put into standing at the room temperature for 30-40 minutes, and then the total yellow liquid is pressed into a mask for clinical application by a metal pressing plate. The implant material provided by the invention is high in yield, and can be naturally formed into gel without adding thrombin and calcium ions; as a result, the use risk of biochemical preparations is avoided; the operation of the implant material is simple; the PRF gel product is thick in fibrous protein filaments, good in elasticity and tenacity, strong in tension, high in hardness, slow in degradation, and not easy to break by tearing; almost no damage is caused in the preparation process of hemameba, blood platelet and shed particles of the two; and after being implanted in vivo, the implant material slowly releases various growth factors and anti-inflammatory cytokines to promote wound healing.

The invention discloses a protein filament extruding device. The protein filament extruding device is characterized in that a V-shaped hopper is arranged above the inside of a box, the lower portion of the V-shaped hopper is provided with an oblique water outlet groove, one end of the water outlet groove extends out of the outer wall of the box, an auger is arranged at the bottom of the V-shaped hopper, the other end of the auger is inserted into an extrusion outer sleeve on the outer side of the box, and the extrusion outer sleeve is provided with an extrusion nozzle through a locking nut. By adopting the arrangement, the protein filament extruding device can extrude moisture contained in soaked protein filaments, enable the moisture contained in the protein filaments to meet the process requirement, reduce the manual-extrusion labor intensity and improve the production efficiency.

The invention discloses a deep-sea fish collagen filament formula and a preparation method thereof. The deep-sea fish collagen filament is prepared by mixing deep-sea fish collagen raw materials, hydrolyzed collagen, seaweed extract, marine shellfish polypeptide and herbal extract, and the deep-sea fish collagen filament is prepared from the following components in parts by weight: 20-25 parts of deep-sea fish collagen raw materials, 2-6 parts of hydrolyzed collagen, 8-12 parts of seaweed extract, 2-4 parts of marine shellfish polypeptide and 1-3 parts of herbal extract. The deep-sea fish collagen filament formula and the preparation method thereof provided by the invention mainly have the advantages of simple preparation process, low raw material cost, high collagen content and convenience to carry and take since the product is in filament form. In addition, water dissolution is performed according to use requirements to carry out nutritional supplement on the skin, thus being wide in effect range, high in permeation and convenient to be absorbed, so that nutritional supplement is fully performed, without dependence. The collagen molecular structure is prevented from being broken by adopting manual sub-packaging, thus maintaining the activity. The deep-sea fish collagen filament formula can make the skin moistened and moisturized, soft and tender and smooth and compact.

The invention discloses a spinning method collagen thread preparation method, and particularly relates to the field of protein thread preparation, the spinning method collagen thread preparation method comprises a main body mechanism, the top of the main body mechanism is fixedly provided with a first processing mechanism, one side of the first processing mechanism is provided with a second processing mechanism, the main body mechanism comprises a base, and the top of the base is fixedly provided with a thread making machine; the interior of the silk making machine is provided with a protein silk strip, the outer wall of the protein silk strip is sleeved with a bending pipe connected with the base, the top of the base is fixedly provided with a winding drum used for winding the protein silk strip, and the first processing mechanism comprises a first connecting seat which is fixedly installed on one side of the base in a vertical state. Protein filaments are subjected to a stretching test between the first heating plate and the second heating plate while being segmented and heated and cured by the first heating plate and the second heating plate, and burrs on the surfaces of the protein filaments are scraped by the scraper, so that the production quality of the protein filaments is improved, and the production efficiency is improved. The practical use is more convenient.