31 results about "Gladiolus" patented technology

The invention discloses a nucleotide sequence and kit for detecting Burkholderia gladioli and belongs to the field of plant inspection and quarantine. Primers of fluorescence quantitative PCR (polymerase chain reaction) for detecting Burkholderia gladioli has oligonucleotide sequences shown in SEQ ID No.1 and SEQ ID No.2. The invention has the advantages that: with the fluorescence quantitative PCR detection method and kit provided by the invention, the detection of Burkholderia gladioli is specific, sensitive and rapid.

The invention discloses a preparation method of a burkholderia gladioli molecular standard sample. The preparation method comprises the step of carrying out freeze drying on extracted genome nucleic acid of burkholderia gladioli, wherein the freeze drying conditions are as follows: a freeze dryer is started, and a cooling trap is started when temperature of a drying chamber is lowered to -35 DEG C; when the temperature of the cooling trap is lowered to -40 DEG C, a nucleic acid sample prefrozen for 2 hours at the temperature of -80 DEG C is put into the drying chamber and then vacuumized; when vacuum degree is reduced to 0.5Torr, freezing is finished; and the sample is dried at the temperature of 15 DEG C, the sample is taken out when the vacuum degree is reduced to 0.1Torr, a penicillin bottle is tightened to obtain a burkholderia gladioli genome nucleic acid standard sample, and the sample is kept in a dark place and stored at the temperature of 20 DEG C. The molecular standard sample disclosed by the invention has good physical and chemical properties and can be detected by adopting a specific primer through PCR process, and sensitivity reaches 10<-4>. Detection test shows that the standard sample has good DNA integrity, uniformity and stability, can be applied to import and export detection as a positive reference material, and accuracy of a detection result is improved.

The invention discloses a protein fingerprint model for Burkholderia gladioli and application thereof. The model is drafted according to the mass-to-charge ratios of the protein of Burkholderia gladioli and the peak intensity coefficients of the protein, wherein the mass-to-charge ratios include 3568.4, 3864.3, 4802.0, 5233.8, 6829.7, 7133.9, 7422.1, 7701.7, 9602.7 and 10153.8. A detection method comprises the following steps: preparation of a sample protein chip; detection with matrix-assisted laser desorption ionization time-of-flight mass spectrometer; and comparison of the protein fingerprint of the sample. The protein fingerprint model can qualitatively detect whether a sample contains Burkholderia gladioli or not, can be used for detection of commercial Burkholderia gladioli and is especially applicable to departments like a port inspection and quarantine part with detection requirements for rapidness, high flux and the like.

The invention discloses a bactericide composition. The bactericide composition contains pyrimethanil and berberine, for 1 part of pyrimethanil, the content of the berberine is 0.01-90 parts by weight. The invention further provides application of the bactericide composition in prevention and control of botrytis of cucumbers, tomatoes, strawberries, grapes, rieger begonia, cyclamen, cineraria, phalaenopsis, poinsettia pulcherrima, peony, China aster, camellia, chrysanthemums, begonia, dahlia, geranium and gladiolus. Through the adoption of the technical scheme, the bactericide composition has obviously enhanced prevention and control effect with respect to the pyrimethanil and berberine.

The invention belongs to the field of plant cultivation technology, in particular to the field of gladiolus cultivation technology. A gladiolus cut flower preservative is characterized by comprising the following components in one liter of water: 50-65 g of sucrose, 0.3-0.8 g of calcium oxalate, 0.008-0.06 g of salicylic acid, 0.06-0.6 g of citric acid and 0.03-0.6 g of cobalt chloride. Through the gladiolus cut flower preservative and preparation and using methods thereof provided by the invention, the gladiolus cut flower has bright flower, does not drop or wilt, and contains sufficient water and balanced nutrition; and moreover, aging of the flower can be postponed, the blossom time is prolonged, the quality of the gladiolus cut flower is ensured, and the market demands are fully met.

The invention discloses a gladiolus crossbreeding method using a sunlight greenhouse. Seeds are sown within the mid-to-end of August and the first ten-day period of September when the outdoor highest temperature in not higher than 29 DEG C; cold protection, heat preservation and ventilation are carried out after seedball treatment, soil treatment, soil preparation, sowing and hauling are carried out; daily management refers to moisture management, humiture management and topdressing; artificial pollination is carried out after flowering at the last ten-day period of November in the same year; and seeds are mature 5-6 weeks later. The gladiolus crossbreeding method can greatly delay the flowering phase, effectively solves the inconsistent flowering phase problems of different varieties, and simultaneously provides enough artificial pollination time for crossbreeding work, reduces workload because bagging is not needed before and after flowering, the operation of stamen removal is only carried out before pollen sacs break, and bagging is also not needed before and after pollination, and greatly increases maturing rate, wherein the maturing rate is over 80% and each capsule contains over 60 grains. By adopting the sunlight greenhouse for crossbreeding, the sowing time is delayed, and seasons with high temperature, high humidity and abundant rainwater are avoided, fruit setting is favored and insect pest is prevented.

The invention discloses a multifunctional compound biofertilizer and belongs to the field of biotechnology. The multifunctional compound biofertilizer comprises fermented liquid of burkholderia gladioli, rahnella aquatilis and azotobacter, having effective functions of phosphorus solubilizing and nitrogen fixation, and can increase effective phosphorus content and nitrogen content needed for growth of plants in soil and improve soil fertility; IAA secreted from the biofertilizer can improve growth of plants; besides, the compound biofertilizer can generate high-activity chitinase, effectivelyinhibit breeding of pathogenic fungus, reduce diseases, further increase and supplement beneficial microorganism of soil and improve status of soil, and thus, the compound biofertilizer has good development prospect in terms of reducing usage of chemical fertilizer, improving soil fertility, improving growth plants and the like.

The invention discloses an out-of-season breeding method in a gladiolus seedball sunlight greenhouse. The sowing time is from last ten days of August to first ten days of September; sowing is carried out when the highest outdoor temperature is lower than 29 DEG C, and the sowing time is postponed for 4-5 months compared with a normal sowing period; from the end of April to the end of May in the second year, when a seedball grows for 8-9 months, the average circumference of the seedball is above 12cm; through a manner of controlling water, a plant is forced to hibernate; when the part of the plant on the ground withers, the seedball is dug; the gladiolus seedball is soaked by 800 times of 40% of methanal or by 0.3% of potassium permanganate, and is dried in the air and placed in a refrigeration house with 2-4 DEG C to be stored for 3 months; and seedballs processed at low temperature and used for production are taken out from the refrigeration house to be planted in batch according to time to market. The out-of-season breeding method in the gladiolus seedball sunlight greenhouse has the advantages of postponing sowing and harvesting periods, reducing the storage time of the seedballs, saving the storage cost, reducing nutrition consumption of the seedballs, and improving the seedball quality.

A preparation method of gladiolus nutrient fertilizer, which is composed of: neem leaf powder, garlic powder, tea dry powder, tripterygium powder, dried duck manure, dried rabbit manure, rape straw powder, chicken blood powder, duck blood powder, sorghum cake flour, cottonseed husk powder, sesame cake powder, sunflower seed powder, buckwheat husk powder, bone meal, multivitamins, pine needle powder, mother-of-pearl powder, the percentages described are percentages by weight; various nutrients contained in the formula promote The grown gladiolus leaves are fat, thick and tender, densely growing, strong in growth, good in light reception, rich in color in the corolla, tall and straight inflorescences, like burning sparks, bright and eye-catching, and has excellent landscape effects.

The invention discloses muscle and bone pain relieving powder, belongs to the field of external traditional Chinese medicine preparations and in particular relates to a traditional Chinese medicine for treating protrusion of intervertebral disc. The muscle and bone pain relieving powder is prepared from the following raw materials in parts by weight: 2-5 parts of gynura aurantiaca, 2-7 parts of tobacco, 5-8 parts of elm, 5-10 parts of hance pepper stem and leaf, 5-13 parts of lindera communis, 3-14 parts of nepal saurauia bark, 4-15 parts of snake grape root, 2-5 parts of asarum, 2-13 parts of artemisia rupestris and 1-10 parts of gladiolus corm. The preparation process comprises the following steps: mixing and grinding the raw materials according to the weight ratio, wherein the granularity is 60 meshes, and bagging for later use. The traditional Chinese medicine can be used for effectively treating slipped disc of cervical vertebra, thoracic vertebra and lumbar vertebra, traumatic injury, fracture and traumatic joints, is a pure Chinese herbal medicine preparation, is safer in oral administration than external use, simple in treatment, fast in response, fast in pain rehabilitation and high in cure rate and does not have any toxic or side effect.

The invention discloses a chemical composition for promoting gladiolus corm to quickly grow as well as a preparation method and an application of the chemical composition. The chemical composition for promoting gladiolus corm to quickly grow comprises 6-BA (Butyl Acrylate) or NAA (Naphthyl Acetic Acid), wherein the concentration of the 6-BA is 0.05mg.L<-1>-0.15 mg. L<-1>; and the concentration of the NAA is 0.05mg.L<-1>-0.15 mg. L<-1>. The method for inducing gladiolus corm to quickly grow comprises the following steps of: pre-treating and cleaning the corm; using the chemical composition disclosed by the invention as a treatment reagent for carrying out penetration treatment; replacing the treatment reagent regularly; and cleaning and planting after the treatment. The chemical composition for promoting gladiolus corm to quickly grow has the beneficial effects as follows: the corm can be quickly and orderly induced to root, so that the bud germination is orderly and consistent; the later-period operation is combined, so that the growth period of the over-ground part of the potted gladiolus is shortened, the later-period seeding rate is effectively improved, the production cost of the gladiolus is lowered, and the operation is simple, and therefore, the chemical composition has a good application prospect for improving economic benefits of the flower industry.

The invention provides a cultivation substrate for gladiolus. The substrate comprises an organic waste substrate material, which serves as a main ingredient, and pearlite which serves as an auxiliary ingredient, wherein the organic waste substrate material is a mixture of two or more kinds of agriculture and forestry organic wastes, which can mutually make up the deficiency of physical and chemical properties of single substrates, improve the physical and chemical properties of the cultivation substrate and are subjected to fermentation treatment separately. According to the mixed substrate provided by the invention, the deficiency of the single substrates can be overcome, the physical and chemical properties of the substrate are improved, the quality of cultivation of the gladiolus is improved, meanwhile, the use level of peat in the soilless culture of flowers and plants is lowered, the resource-converting utilization of the agriculture and forestry organic wastes such as wheat straws, maize stalks and peanut shells is realized, and the production cost is reduced greatly, so that the cultivation substrate for the gladiolus has obvious social, economic, environmental and ecological benefits.

The invention discloses a method for cutting ball propagating of gladiolus. In step 1, a spheroidal seed ball with no stalk, no diseases and pests and no damage is selected; in step 2, the skin film of the gladiolus seed ball is removed, washed in the water to wash off the surface layer dust, soil and dirt, then soaked for 10-30 minutes with a 70% methyl thiophanate powder solution, and then takenout, spread and dried; in step 3, the spheroidal seed ball is cut into 4-8 pieceswith a knife according to the number of buds on the gladiolus seed ball, and each piece has at least one complete budor cryptand some root plates; in step 4,the cut seed ball is planted in a small seedling pot,1-2cm of soil is covered, and the seed ball is placed in a greenhouse for cultivation, when the leaves of gladiolus are grown, the leaves are grown to a size of 5 cm or more, and the seedlings are transferred to a large seedling pot or planted in the ground for cultivation. Themethod for the cutting ball propagating of the gladiolusimproves the efficiency of seedling raising, meets the needs of commercial seedling breeding and germplasm resources protection of the gladiolus, and improves the breeding efficiency of gladiolus seedlings.

The invention discloses a chemical fertilizer for Gladiolus and a preparation method thereof. The chemical fertilizer for Gladiolus comprises the following components in parts by mass: 80-100 parts of mink manure, 40-60 parts of cattle manure, 100-120 parts of corn straw, 30-50 parts of Japanese sweetflag, 20-40 parts of Achnatherum inebrians, 15-17 parts of Laminaria digitata residue, 2-4 parts of phosphate solubilizing bacteria, 12-14 parts of pepper powder, 10-12 parts of peanut cake, 0.7-0.9 part of effective microorganism solution, 1-3 parts of ferment and 200-260 parts of water. The chemical fertilizer for Gladiolus can provide sufficient and stable nutrients for the plants, and does not need excessive supplementary fertilizers in the plant growth period.

This invention involves a bottle of liquor for the pretreatment of TangChangPu cut flowers. The character is that the pretreatment liquor is made up of 8-hyoloxide quindine, citric acid, gibberellin, second diamine four acetic acid natrium, amrita candy. Using the pretreatment liquor of This invention discloses good for the come out of TangChangPu flowers and delay wither of flowers.

The invention is a business method of producing genetically unique plants such as Gladiolus, with each unique variety including bulbs for propagation, and offering a customer the opportunity to name the new variety of plant in memory of or in honor of a person of his choice.

A novel and distinct cultivar of Gladiolus plant named `Anurag` particularly characterized by the combined features of light reddish purple (RHS-74D) flowers with dark reddish purple (RHS-66A) color blotches at both sides of the petals; at least 1.7 spikes per plant; 120 cm long flower spike; and approximately 16 flowers per spike.

The invention discloses a traditional Chinese medicinal external lotion for treating infective wounds caused by multi-drug resistant bacteria. According to traditional Chinese medicine, treatment based on syndrome differentiation is conducted in accordance with different symptoms of deficiency and excess, cold and heat, blood stasis and edema for the pathogenesis causing the infective wounds due to multi-drug resistant bacteria. The external lotion disclosed by the invention is prepared from the following traditional Chinese medicines: indian stringbush root, yellow bedstraw herb, Chinese crinum leaves, breeders gladiolus corm, wedelia chinensis, pyrolusite, circaeashape loosestrife herb, gelsmium elegans, kerr treebine keaf, euphorbia antiquorum, beautiful galangal seeds and herba eupatorii. Upon clinical tests, the total effective rate reaches 95%; and the external lotion is free from cases of cross infection and is relatively good in curative effect.

The invention discloses a bactericide composition. The bactericide composition contains pyrimethanil and berberine, for 1 part of pyrimethanil, the content of the berberine is 0.01-90 parts by weight. The invention further provides application of the bactericide composition in prevention and control of botrytis of cucumbers, tomatoes, strawberries, grapes, rieger begonia, cyclamen, cineraria, phalaenopsis, poinsettia pulcherrima, peony, China aster, camellia, chrysanthemums, begonia, dahlia, geranium and gladiolus. Through the adoption of the technical scheme, the bactericide composition has obviously enhanced prevention and control effect with respect to the pyrimethanil and berberine.

The invention relates to a high-flexibility synthetic leather sizing agent and a preparation method thereof. The high-flexibility synthetic leather sizing agent comprises the following components in parts by mass: 80-95 parts of solvent type polyurethane resin, 6-8 parts of 3,3-disulfonhydrazide diphenyl sulfone, 4-5 parts of fatty acid amide, 2-4 parts of epoxy resin, 10-15 parts of gladiolus leaves, 5-8 parts of akanthikon powder, 1-2 parts of dibutyl phthalate, 20-35 parts of acetone and 25-28 parts of bentonite. The synthetic leather sizing agent with the solvent type polyurethane resin as a main body is fine and smooth, a finished product of synthetic leather is flexible and natural, is soft in hand feeling, has an appearance of good real leather features, and is good in elasticity, excellent in adhesion property and good in abrasion resistance, deflection resistance, aging resistance and mold resistance. After fibers are implanted, the connection strength of the synthetic leather can be enhanced, and the problem that polyurethane resin is too soft is solved, the texture of the synthetic leather is better, and the prepared synthetic leather is approximate to natural leather in quality.

The invention discloses a gladiolus crossbreeding method using a sunlight greenhouse. Seeds are sown within the mid-to-end of August and the first ten-day period of September when the outdoor highest temperature in not higher than 29 DEG C; cold protection, heat preservation and ventilation are carried out after seedball treatment, soil treatment, soil preparation, sowing and hauling are carried out; daily management refers to moisture management, humiture management and topdressing; artificial pollination is carried out after flowering at the last ten-day period of November in the same year; and seeds are mature 5-6 weeks later. The gladiolus crossbreeding method can greatly delay the flowering phase, effectively solves the inconsistent flowering phase problems of different varieties, and simultaneously provides enough artificial pollination time for crossbreeding work, reduces workload because bagging is not needed before and after flowering, the operation of stamen removal is only carried out before pollen sacs break, and bagging is also not needed before and after pollination, and greatly increases maturing rate, wherein the maturing rate is over 80% and each capsule contains over 60 grains. By adopting the sunlight greenhouse for crossbreeding, the sowing time is delayed, and seasons with high temperature, high humidity and abundant rainwater are avoided, fruit setting is favored and insect pest is prevented.

A novel and distinct cultivar of Gladiolus plant named `Anurag` particularly characterized by the combined features of light reddish purple (RHS-74D) flowers with dark reddish purple (RHS-66A) color blotches at both sides of the petals; at least 1.7 spikes per plant; 120 cm long flower spike; and approximately 16 flowers per spike.

The invention discloses a chemical composition for promoting gladiolus corm to quickly grow as well as a preparation method and an application of the chemical composition. The chemical composition for promoting gladiolus corm to quickly grow comprises 6-BA (Butyl Acrylate) or NAA (Naphthyl Acetic Acid), wherein the concentration of the 6-BA is 0.05mg.L<-1>-0.15 mg. L<-1>; and the concentration of the NAA is 0.05mg.L<-1>-0.15 mg. L<-1>. The method for inducing gladiolus corm to quickly grow comprises the following steps of: pre-treating and cleaning the corm; using the chemical composition disclosed by the invention as a treatment reagent for carrying out penetration treatment; replacing the treatment reagent regularly; and cleaning and planting after the treatment. The chemical composition for promoting gladiolus corm to quickly grow has the beneficial effects as follows: the corm can be quickly and orderly induced to root, so that the bud germination is orderly and consistent; the later-period operation is combined, so that the growth period of the over-ground part of the potted gladiolus is shortened, the later-period seeding rate is effectively improved, the production cost of the gladiolus is lowered, and the operation is simple, and therefore, the chemical composition has a good application prospect for improving economic benefits of the flower industry.

The invention discloses muscle and bone pain relieving powder, belongs to the field of external traditional Chinese medicine preparations and in particular relates to a traditional Chinese medicine for treating protrusion of intervertebral disc. The muscle and bone pain relieving powder is prepared from the following raw materials in parts by weight: 2-5 parts of gynura aurantiaca, 2-7 parts of tobacco, 5-8 parts of elm, 5-10 parts of hance pepper stem and leaf, 5-13 parts of lindera communis, 3-14 parts of nepal saurauia bark, 4-15 parts of snake grape root, 2-5 parts of asarum, 2-13 parts of artemisia rupestris and 1-10 parts of gladiolus corm. The preparation process comprises the following steps: mixing and grinding the raw materials according to the weight ratio, wherein the granularity is 60 meshes, and bagging for later use. The traditional Chinese medicine can be used for effectively treating slipped disc of cervical vertebra, thoracic vertebra and lumbar vertebra, traumatic injury, fracture and traumatic joints, is a pure Chinese herbal medicine preparation, is safer in oral administration than external use, simple in treatment, fast in response, fast in pain rehabilitation and high in cure rate and does not have any toxic or side effect.

The invention provides a gladiolus culture medium. The gladiolus culture medium comprises an organic waste medium material serving as a main component, and perlite serving as an auxiliary component. The organic waste medium material is a mixture of two or more kinds of fermented agriculture and forestry organic waste capable of mutually compensating for the defects of the single medium physical and chemical properties and improving the physical and chemical properties of the culture medium. The mixed medium can overcome the defects of a single medium, the physical and chemical properties of the medium are improved, and the cultivation quality of the gladiolus is improved. Meanwhile, the usage amount of peat in flower soilless culture, and resource utilization of wheat stems, maize straw, peanut shells and other agriculture and forestry organic waste is achieved; and the production cost is greatly reduced, and obvious social benefits, economic benefits and environment and ecology benefits are achieved.

The invention discloses an out-of-season breeding method in a gladiolus seedball sunlight greenhouse. The sowing time is from last ten days of August to first ten days of September; sowing is carried out when the highest outdoor temperature is lower than 29 DEG C, and the sowing time is postponed for 4-5 months compared with a normal sowing period; from the end of April to the end of May in the second year, when a seedball grows for 8-9 months, the average circumference of the seedball is above 12cm; through a manner of controlling water, a plant is forced to hibernate; when the part of the plant on the ground withers, the seedball is dug; the gladiolus seedball is soaked by 800 times of 40% of methanal or by 0.3% of potassium permanganate, and is dried in the air and placed in a refrigeration house with 2-4 DEG C to be stored for 3 months; and seedballs processed at low temperature and used for production are taken out from the refrigeration house to be planted in batch according to time to market. The out-of-season breeding method in the gladiolus seedball sunlight greenhouse has the advantages of postponing sowing and harvesting periods, reducing the storage time of the seedballs, saving the storage cost, reducing nutrition consumption of the seedballs, and improving the seedball quality.

The invention discloses a moisture determination method for fresh cut flowers of gladiolus. The invention provides a method for measuring moisture in the senescence process of fresh cut flowers by combining low-field nuclear magnetic imaging and T2 relaxation method, and provides a standardized method for studying the physiological mechanism of fresh cut flowers senescence, developing fresh-keeping technology and delaying their senescence. The method requires less sample volume and simple steps, overcomes the long time-consuming detection of traditional drying methods, and cannot meet the requirements of real-time feedback and real-time adjustment in the production process; and the measurement speed is fast, the signal-to-noise ratio is good, and the prediction results are stable and accurate. Identification, optimization, research and application of fresh-cut flower preservation technology.

The invention relates to the technical field of flower planting, in particular to a residual branch regeneration method of gladiolus. The gladiolus of which flowers are cut are selected as regeneration female parents, when leaves of the dry gladiolus female parent become yellow, earth of open spaces on the periphery of roots of the gladiolus is turned 3-5 cm, weeds are removed, 350-400 kg of rapeseed cake powder is applied to each mu of earth, a stainless steel cutting edge is selected to prune stem leaves of the gladiolus along the roots of the gladiolus, a segment of main stems with the length of 0.5-1 cm remains, tissue culture of the main stems continues, and in combination with a specially prepared nutrient solution and later planting management conditions suitable for growth of thegladiolus, old gladiolus plants continue to sprout, grow and bloom again. It only takes four months from the beginning of the tissue culture of the old gladiolus plants to blooming of the old gladiolus plants, full use of the old gladiolus plants is effectively achieved, the planting cost of farmers is reduced, the residual branch regeneration method of the gladiolus is economical and practical, and the bran-new planting method is provided for gladiolus planting.

The invention discloses a gladiolus tissue culture matrix. The matrix comprises agar, and is characterized by also comprising mashed banana, corn starch, carbonized rice husk, and mud in the biogas generating pit. The provided gladiolus tissue culture matrix can improve the living rate of gladiolus to 88% or more, reduces the damage by diseases and insects, and is capable of shortening the seedling emergence time by 18% and reducing the rooting and exuberance time by 45%, compared with common tissue culture matrixes.

The invention discloses a gladiolus flower-shaped NiSe / CoSe / Ni 3 Se 2 The nanocomposite array electrode material and its preparation method belong to the technical field of supercapacitor electrode material preparation. It is prepared by the following steps: adding cobalt acetate tetrahydrate into a mixed solvent of anisole and methanol, heating and dissolving to form a mixed reaction solution, moving it into a reaction kettle, and then putting it into the prefabricated Ni 3 Se 2 Nanowire array precursor, heat treatment after sealing the reactor, synthesis of gladiolus-like NiSe / CoSe / Ni with both one-dimensional and two-dimensional structural characteristics 3 Se 2 Nanocomposite array electrode materials. The obtained electrode material has excellent electrochemical performance, with a specific capacitance of 1666 F / g at a current density of 0.5 A / g, and a specific capacitance of 944 F / g even at a current density of 2.5 A / g. .