64 results about "Nannochloropsis" patented technology

The present invention provides commercially viable, large-scale methods for concentrating microalgae with an average diameter of about 20 μm or less. The methods find use in concentrating microalgae with an average diameter of about 5 μm or less, for example, Nannochloropsis.

Provided herein are exemplary vectors for transforming algal cells. In exemplary embodiments, the vector comprises a Violaxanthin-chlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell. Embodiments of the invention may be used to introduce a gene (or genes) into the alga Nannochloropsis, such that the gene(s) are expressed and functional. This unprecedented ability to transform Nannochloropsis with high efficiency makes possible new developments in phycology, aquaculture and biofuels applications.

Provided herein are exemplary methods for the use of 2-hydroxy-5-oxoproline in conjunction with algae. One exemplary method includes applying an effective amount of 2-hydroxy-5-oxoproline to algae in an aqueous environment to accelerate creation of a high-cell density of the algae. The effective amount of the 2-hydroxy-5-oxoproline may be approximately 0.1 grams per liter of the aqueous environment, or up to approximately 0.1 grams per liter of the aqueous environment. The effective amount of the 2-hydroxy-5-oxoproline may be applied to the aqueous environment at or near a same time, or applied to the aqueous environment over a period of time. Exemplary algae cultivation systems are also provided herein. One exemplary system includes an aqueous environment having a pale-green mutant Nannochloropsis, and an effective amount of 2-hydroxy-5-oxoproline to accelerate creation of a high-cell density of the pale-green mutant Nannochloropsis.

The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. More specifically, the invention relates to a Nannochloropsis algal strain and mutants that produces high amounts of C16 fatty acids and hydrocarbons. The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. More specifically, the invention relates to a Nannochloropsis algal strain and mutants that produces high amounts of C16 fatty acids and hydrocarbons.

The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in Nannochloropsis and other microalgae.

Methods for controlling a density of algae growing in an aquatic environment are provided. Exemplary methods include applying an effective amount of glyphosate to a density of algae growing in an aquatic environment. The algae may include genus Nannochloropsis and / or Dunaliella. The algae may also include a glyphosate resistant strain of genus Nannochloropsis. The effective amount may result in an approximate concentration of between 0.1 millimolar to 0.3 millimolar glyphosate in the aquatic environment. Additionally, the aquatic environment may include seawater. The glyphosate may be applied to the aquatic environment before and / or after the aquatic environment is inoculated with algae. Alternative methods include applying an effective amount of glufosinate to a density of algae growing in an aquatic environment.

Systems and methods for maintaining the dominance of Nannochloropsis in an algae cultivation system are provided. Exemplary methods include applying an effective amount of a disinfectant to Nannochloropsis growing in an algae cultivation system. Another method for maintaining the dominance of Nannochloropsis in an algae cultivation system includes adjusting a salinity in the algae cultivation system to between approximately 0.5 PPT and 28 PPT. In a further method, temperature of the algae cultivation system may be adjusted to between approximately 21 and 32 degrees Celsius (“° C.”). According to yet another method for maintaining the dominance of Nannochloropsis in an algae cultivation system, a salinity in the algae cultivation system may be adjusted to below that of seawater for a first predetermined period of time, and then the salinity in the algae cultivation system may be adjusted to a higher salinity for a second predetermined period of time.

Transgenic plants are more tolerant to environmental stresses than untransformed plants. The pea ABR17 (Abscisic acid responsive 17) is used to enhance germination of plants such as Arabidopsis sp. and Brassica sp. while under multiple abiotic stresses, and to enhance the tolerance of these plants to these stresses. Three independently derived Arabidopsis transgenic lines, containing ABR17, germinated better in the presence of salt, cold temperature or both. The transgenic plants also exhibited enhanced tolerance to freezing temperature or extreme heat. Furthermore, the transgenic plants demonstrated early flowering even under normal, non-stressed conditions.

Systems and methods for maintaining the dominance and increasing the biomass production of Nannochloropsis in an algae cultivation system are provided. Exemplary methods include applying an effective amount of ozone to Nannochloropsis growing in an algae cultivation system. A further method may include applying a shock amount of ozone above 10 milligrams / liter or higher in the inlet stream flowing into the algae cultivation system. Various exemplary embodiments may include a system for maintaining dominance and increasing the biomass production of Nannochloropsis in an algae cultivation system. The system may comprise a processor, and a computer readable storage medium having instructions for execution by the processor. The instructions for execution by the processor cause the processor to maintain dominance and increase biomass production of the Nannochloropsis in the algae cultivation system.

The invention provides an arabidopsis hydroponic method capable of effectively preventing generation of green algae. The method comprises the following steps: 1, raising a seedling, wherein sterilized and vernalized seeds are sowed in 1 / 2 MS solid medium in a cultivation bottle and cultivated for 12-18 days to obtain a seedling; 2, transferring the seedling, wherein the seedling obtained from the step 1 is transferred to a small KT plate which has an area of 3-5 square centimeters and is provide with a hole, the roots penetrate into a nutrient solution through the hole and are acclimated and cultivated for 5-7 days; 3, self-making and disinfecting a cultivation device, and sealing the cultivation device by a piece of tin foil paper; and 4, cultivating the seedling, wherein the seedling on the small KT plate is transferred to the self-made cultivation device, the roots of the arabidopsis are immersed into the cultivation solution through the piece of tin foil paper and the cultivation device, and the cultivation solution is changed once every 6-9 days and is ventilated once every 2-3 days so as to ensure enough oxygen at the root area. The cultivation area provided by the invention is simple and easy to implement. Moreover, the arabidopsis grows well and the green algae can not be generated.

The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chlorella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.

The invention relates to the field of vitrification ultra-low temperature storage, in particular to a screening method of an allogenic material for promoting vitrification ultra-low temperature storage; an arabidopsis thaliana seedling is adopted as an experimental object; the allogenic material is added into vitrification solution for the vitrification ultra-low temperature storage of the arabidopsis thaliana seedling; the arabidopsis thaliana seedling which is not added with the allogenic material is adopted as a contrast; and the effect of the allogenic material is evaluated by comparing the recovery rate. According to the screening method of the allogenic material for promoting vitrification ultra-low temperature storage, the result is accurate, the repeatability is strong, the scope of application is wide, batch screening can be carried out, the efficiency is high, an effective approach is provided for screening the allogenic material, technical guarantee is provided for a vitrification ultra-low temperature storage technology, and a guiding role is played to the storage of plant species resources.

The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chlorella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.

The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and / or isolation of nucleic acid molecules tagged with a spliced leader sequence.

The invention provides an application of a cytochalasin category compounds in a herbicide and belongs to the technical field of pharmaceutical chemistry. According to the invention, the cytochalasin category compounds includes a compound with any one structure shown in the formula AL-11-18 or a physiologically acceptable salt thereof. The cytochalasin category compounds have phytotoxicity and canbe used as an effective component of a herbicide. Example results show that the cytochalasin category compounds have an inhibition effect on arabidopsis thaliana rooting.

The present invention belongs to the technical field of biology, and relates to a gene having a triacylglycerol (TAG) synthesis function, and applications thereof, wherein the gene is the following nucleotide sequence: 1) the gene is base sequence represented by SEQ ID NO1; or, 2) the gene is the DNA sequence sharing more than or equal to 95% of homology with the nucleic acid sequence limited by the sequence 1 in the sequence list and encoding the same biological function protein. According to the present invention, the gene having the TAG synthesis function is the diacylglycerol acyltransferase (DGAT) gene isolated from Nannochloropsis, and has important application values in the improvement of the TAG content in the organism.

Systems and methods for maintaining the dominance of Nannochloropsis in an algae cultivation system are provided. Exemplary methods include applying an effective amount of a disinfectant to Nannochloropsis growing in an algae cultivation system. Another method for maintaining the dominance of Nannochloropsis in an algae cultivation system includes adjusting a salinity in the algae cultivation system to between approximately 0.5 PPT and 28 PPT. In a further method, the temperature of the algae cultivation system may be adjusted to between approximately 21 and 32 degrees Celsius (“° C.”). According to yet another method for maintaining the dominance of Nannochloropsis in an algae cultivation system, a salinity in the algae cultivation system may be adjusted to below that of seawater for a first predetermined period of time, and then the salinity in the algae cultivation system may be adjusted to a higher salinity for a second predetermined period of time.

Methods of using genetically-transformed plants in the phytoremediation of lead are described. Unlike many organisms in which only 10-kDa ACBPs have been identified, there exists a family of six ACBPs in the model plant Arabidopsis. Other than a function in mediating the transfer of acyl-CoA esters in plant lipid metabolism, all six Arabidopsis ACBPs can bind the heavy metal lead and are therefore applicable for phytoremediation. These methods of phytoremediation will provide a cheap, simple and efficient method in the removal of contaminating lead from soil / water / environment by the growth of the ACBP-overexpressing genetically-transformed plants in the contaminated environment. There is also provided a method to remove lead from contaminated water.

The invention relates to the field of polymer production, in particular to the production of chitin and chitosan from microalgae belonging to the phylum Haptophyta to the phylum Chlorophyta, or to the phylum Heterokontophyta, particularly from microalgae of the genus Isochrysis, Chlorella, Bracteacoccus, Chlorococcum, Scenedesmns, Desmodesmus, Haematococcus, Thalassiosira and Nannochloropsis, as well as to microalgal extracts thereof and their uses.

The invention belongs to the technical field of biology. The invention relates to a gene with a triglyceride synthesis function and a construction method thereof and an application thereof for rationally regulating contents of oil-producing microalgae triglyceride and polyunsaturated fatty acid. The gene is: 1) a base sequence shown as SEQ ID NO: 1 or 2; or 2) a DNA sequence of the protein encoding the same biological function and having 95% or more homology with the nucleic acid sequence defined by a sequence 1 or 2 in a sequence table. The full-length gene and the amino acid sequence as nannochloropsis are firstly reported, and the members of the DGAT gene family can be enriched; overexpression of the above gene can significantly improve the ability of nannochloropsis to synthesize TAG,and the application value in using genetic engineering to improve the production of TAG in organisms can be improved; in addition, overexpression of the above gene can increase the specific PUFA content in the TAG, and therefore the method has the potential to increase the yield of a high value-added compound of microalgae.

The invention belongs to the biological technical field, and relates to a gene with a triacylglycerol (TAG) synthesis function and the application of the gene in increase of the TAG content and rational regulation of the saturation degree of microalgae TAG. The gene is a base sequence as shown in SEQ ID NO 1, a base sequence as shown in SEQ ID NO 2 or a base sequence as shown in SEQ ID NO 3, and aDNA sequence which is homologous with the base sequence as shown in SEQ ID NO 1, the base sequence as shown in SEQ ID NO 2 or the base sequence as shown in SEQ ID NO 3 by 95 percent or above and encodes identical biological functional protein. The gene disclosed by the invention comprises three DGAT (diacylglycerol acyltransferase) genes separated from nannochloropsis. The obtained gene is used for rationally regulating the content and the saturation degree of TAG. Biodiesel, a health care product, a medicine and the like have demands for saturation degree of intracellular triglyceride to different extents.

The invention discloses a preparation method of eicosapentaenoic acid (EPA)-containing nannochloropsis oil. The method includes the following steps: step 1, performing centrifugation on a nannochloropsis cell culture liquid in a centrifuge at a rotating speed of 4,000 to 8000 r / min for 20-40 min to obtain a nannochloropsis microdehydrated solution; step 2, performing spray drying treatment on thenannochloropsis microdehydrated solution to obtain dried nannochloropsis powder; step 3, mixing the dried nannochloropsis powder and a decoloring solution, and performing a reaction at 30-80 DEG C for60-240 min to obtain a nannochloropsis decolorized solution; step 4, performing low-temperature vacuum evaporation on the nannochloropsis decolorized solution to remove ethanol to obtain nannochloropsis decolorized dried powder; and step 5, performing subcritical extraction on the nannochloropsis decolorized dried powder to obtain the EPA-containing nannochloropsis oil. The preparation method ofthe EPA-containing nannochloropsis oil is safe in process and convenient in preparation.

The present invention relates to a newly developed immediate chromatin immunoprecipitation procedure (“ZipChIP”). ZipChIP significantly reduces the time and increases sensitivity allowing for rapid screening of multiple loci. ZipChIP enables the detection of histone modifications (e.g., H3K4 mono- and trimethylation) and at least two yeast histone demethylases, Jhd2 and Rph1, which were previously found difficult to detect using standard methods. ZipChIP further relates to the enrichment of the histone deacetylase Sir2 at heterochromatin in yeast and enrichment of the chromatin remodeler, PICKLE, in Arabidopsis thaliana.

The invention discloses feed capable of preventing and controlling loach saprolegniasis and a feeding device thereof, and belongs to the field of feed production. The feed is prepared by mixing goat blood, yellow mealworm, bone tankage, pork liver, phaeodactylum tricornutum bohlin power, dicrateria zhanjiangensis powder, nannochloropsis oculate powder, Chinese herbal medicine compounds and the like. The fragrance is intensive; the food calling effect is good; the digestion is easy; the palatability is high; the manufacturing process equipment is simple and convenient; the feed loss is little;the water body pollution is small; the loach growth is fast; the feeding is convenient; the raw materials can be easily obtained; the feed cost is low; the feeding device is simple and practical; better prevention and control effects on the saprolegniasis are achieved.

The invention relates to the technical field of genetic engineering, in particular to a rich-lipid nannochloropsis chloroplast transgenic system and application thereof. A carrier of the system comprises at least one promoter, a terminator, an upstream homologous arm and a downstream homologous arm, wherein the upstream homologous arm has a base sequence shown as SEQ ID NO:1; the downstream homologous arm has a base sequence shown as SEQ ID NO:2; and a base sequence, shown as SEQ ID NO:7, forming a polycistron structure with at least one exogenous gene is inserted between the homologous arms.By using the rich-lipid nannochloropsis chloroplast transgenic system provided by the invention, the stable expression of a plurality of exogenous genes in chloroplasts can be realized.

The invention discloses a method for extracting EPA in Nannochloropsis algae by a compound enzyme method. The method comprises: centrifuging the fermented liquid of Nannochloropsis algae to collect the algae mud; adding a certain amount of buffer solution to the algae mud, and then adding Composite enzymes, react to obtain enzymolysis solution; centrifuge the enzymolysis solution to obtain the first free oil and emulsion, further demulsify the emulsion and centrifuge to obtain the second free oil, combine the first free oil and the second free oil to obtain Crude oil; the resulting crude oil is subjected to supercritical CO 2 Purification method Further purification to obtain EPA. The present invention treats Nannochloropsis with complex enzymes, and under certain reaction conditions, enzymatically destroys the microalgae cell structure, and at the same time strengthens the degradation of complexes such as lipopolysaccharide and lipoprotein, increases the fluidity of oil, and frees oil . The extraction purity of the EPA of the present invention is above 95%.

The invention discloses a high-density continuous culture method of nannochloropsis. The method comprises the following steps: preparing a culture medium in a closed photoreactor, performing sterilization by using an ultraviolet lamp for 30 min, and inoculating the nannochloropsis grown to an exponential growth phase into the above culture medium, wherein the inoculation amount of the nannochloropsis culture solution is 5-20%; and performing culture by using an artificial LED light source, wherein the culture temperature is 20-30 DEG C, performing irradiation by using monochromatic white lightfor 3-5 days, wherein the irradiation intensity of the white light is 500-2000 lx, when the culture is performed to a fifth day, releasing half of the culture solution, adding half of a fresh culturemedium through flowing, continuing fermentation, and afterward releasing half of volume every 2 days as a fermentation liquid of the nannochloropsis, wherein 10 batches are continuously added. The high-density continuous culture method of the nannochloropsis can continuously provide a light source for the culture of the nannochloropsis, so that the nannochloropsis can be subjected to high-densityculture, the culture work of the nannochloropsis can be continuously carried out, and the continuous culture of the nannochloropsis can be completed.