30 results about "Aspergillus glaucus" patented technology

The invention provides a processing method of a big leaf 'post-fermented tea' dark green tea. The processing method is characterized by comprising the following steps: big leaf fresh leaf picking, water removing, rolling, drying, crude tea drying, classifying and bunching, sterilizing, inoculating, pile fermenting, turning and cooling, air drying and alcoholizing, vapor-pressing, airing, ageing, finished dark green tea; the inoculating refers to access eurotiaceae, and the eurotiaceae is the mixed thallus of aspergillus niger, rhizopus delemar, penicillium sp., aspergillus glaucus and brettanomyces intermedius; the mixed thallus is prepared from the following steps: every strain is subjected to liquid culture, is dispersed into hyphal bodies of 0.2-0.4 cm or so, suspended until 107-108 hyphal bodies or somatic cells are formed per milliliter with the volume ratio of 3: 1: 1: 8: 3, and mixed uniformly.

The invention provides a microbial fertilizer for reducing residues of pesticides and heavy metals and a preparation method of the microbial fertilizer. The fertilizer is prepared from the raw materials by weight: 50kg of carboxymethyl starch, 80kg of flavobacterium oryzae fermentation liquid, 100kg of aspergillus glaucus fermentation liquid, 100kg of bacillus subtilis fermentation liquid, 3kg of chitosan, 2kg of nucleotide, 20kg of potassium nitrate, 20kg of dipotassium hydrogen phosphate, 50kg of potassium humate and 1500kg of water. The microbial fertilizer provided by the invention is simple in preparation method and capable of remarkably reducing the residues of heavy metals and pesticides in agricultural products and improving the qualities of the agricultural products.

The invention relates to a preparation method of Pu'er tea fermentation seed-starter, belonging to the Pu'er tea processing technical field. The preparation method comprises: processing ground mass, inoculating, cultivating and freeze-drying; concretely: a: crushing the tea-leaf ground mass into tea powder of 40mesh; sterilizing for 25min in a process retort at 0.1MPa and 121degree; natural cooling to room temperature; b: processing Aspergillus niger, Aspergillus glaucus and common penicillium into a PDA liquid medium suspension liquid with spore number ratio of 1:1:1 using conventional method and controlling the concentration of the zymogen being 105-107CFU / g after inoculating and controlling the ratio of tea leaf to PDA liquid culture being 4:1; c: inoculating on the PDA culture medium at 30degree for 40h; d: freeze vacuum drying the cultured seed-starter germ at -18degree until the water content is 8-10%, therefore the seed-starter is obtained. The preparation method has features of simple preparation method, low cost, convenient use and is favourable to increase the Pu'er tea quality and especially suitable for large-scale Pu'er tea production.

The invention belongs to the technical field of grain storage, and discloses a bacillus subtilis strain having broad-spectrum antagonism for main grain storage hazard-moulds, a preparation method of an antifungal metabolite of the bacillus subtilis strain, and application of the bacillus subtilis strain and the antifungal metabolite in grain storage. The strain is bacillus subtilis LM3403, and has a preservation number of CGMCC No. 9377. The bacillus subtilis LM3403 has a better inhibiting effect for the main grain storage hazard-moulds such as fusarium graminearum, fusarium moniliforme, aspergillus flavus, aspergillus niger, aspergillus glaucus, aspergillus fumigatus, penicillium oxalicum, penicillium expansum and mucor mucedo, and is wide in antibacterial spectrum, thus having a giant application potential. The antifungal metabolite of the bacillus subtilis LM3403 has an obvious inhibiting effect for the growth of the main grain storage hazard-moulds. The storage test results prove that after the antifungal metabolite of the bacillus subtilis LM3403 is added into stored grains, the growth of the moulds can be effectively inhibited in a grain storage process, so that the grain storage safety is guaranteed.

The invention discloses pine needle soft feed for reducing fish dermatopathy and a preparation method thereof. The pine needle soft feed comprises, by weight, 50-70 parts of fresh pine needle, 14-17 parts of tofu residue, 9-11 parts of corn flour, 0.4-0.6 parts of calamus leaf, 0.3-0.5 parts of aspergillus glaucus liquid, 0.2-0.3 parts of radix sophorae flavescentis extract, 3-5 parts of molasses, 0.5-0.8 parts of a candida utilis liquid, 0.3-0.5 parts of fermented soya bean, 0.1-0.2 parts of ginger oil and 0.3-0.5 parts of edible gelatin. The pine needle soft feed is soft, has good palatability and a thick fragrance, can improve fish immunity and bacterium, fungus and protozoon resistance, can reduce fish saprolegniasis, white skin disease, red skin disease, gill rot and leprnorthsis and improves a fish culture survival rate and income.

The invention discloses a pine leaf soft feed for reducing fish fishiness and a preparation method thereof. The pine leaf soft feed comprises the following raw materials by weight part: 50-70 of fresh pine leaf, 15-18 of sweet potato powder, 12-16 of barley bran, 0.6-0.8 of citrus peel powder, 0.3-0.5 of Aspergillus glaucus bacterial liquid, 4-5 of minced pumpkin, 1-2 of spinach slurry, 0.5-0.8 of Candida utilis bacterial liquid, 0.2-0.3 of Chinese prickly ash water, 0.1-0.2 of a pomegranate peel extract, and 0.3-0.5 of edible gelatin. The feed provided by the invention is soft, has excellent palatability and strong aromatic smell, and can accelerate the metabolism and discharge of waste in fish and discharge, reduce oxidative decomposition of amino acids and fat, reduce fish fishiness, and improve the delicious degree of fish and fish soup.

The invention discloses a processing technology based on fermentation of tea containing clerodendranthus spicatus herbs and poria cocos, and relates to the technical field of tea technologies. The processing technology comprises the following steps of preparation of a tea juice strain of aspergillus glaucus, treatment of tea raw materials, treatment of tea containing clerodendranthus spicatus herbs, steaming inoculation and fermentation, drying and packaging; a culture medium is prepared from 20-30 parts of a yeast extract, 1-3 parts of potato powder, 20-50 parts of glucose, 10-30 parts of Czapek agar and 15-25 parts of a K2HPO4 solution, the fermentation agent culture medium is inoculated with the aspergillus glaucus after high-temperature steam sterilization, and high-temperature disinfection is conducted for 10-15 minutes at 0.110-0.120 MPa and 121 DEG C. The processing technology based on the fermentation of the tea containing the clerodendranthus spicatus herbs and poria cocos hasthe advantages that the strong functions of promoting digestion, lowering blood fat, dissolving fat, regulating sugar metabolism, promoting gastrointestinal movement and weight loss and inhibiting bacteria, saccharomyces cerevisiae, mold and actinomycetes are achieved, the production of metabolic substances of food pathogenic bacteria and spoilage bacteria is inhibited, and the functions of promoting diuresis, removing urinary calculi, preventing oxidation, lowering blood pressure, diminishing inflammation, stopping pain and the like are achieved.

The invention provides a novel culture medium using marine aspergiolide glaucus to manufacture a novel antineoplastic active compound. Per liter of the novel culture medium comprises 20 to 100g of tragantine, 10 to 100g of cane sugar, 20 to 100g of maltose, 0.5 to 6g of peptone, 0.3 to 6g of yeast extract, 0.2 to 4g of soybean flour, 0.5 to 10g of sodium glutamate, 0.01 to 1g of heptahydrate magnesium sulfate, 0.01 to 2g of monopotassium phosphate and the balance being artificial seawater. A method for preparing the novel culture medium is also provided. The novel culture medium using the marine aspergiolide glaucus to manufacture the novel antineoplastic active compound has the advantages of easily received raw materials, low cost and simple preparing method, can obviously improve the yield of griseoviridin A produced and fermented by the marine aspergiolide glaucus HB1-19, improve the yield of the griseoviridin A from the prior 15mg / L to 71mg / L, is advantageous to industrial production in large scale, and has significance for the fermentation investigation of the marine aspergiolide glaucus HB1-19 and the medicine and pharmacology investigation of the griseoviridin A.

The invention relates to a method for increasing yield of griseoviridin A by adding metal ions, belonging to the technical field of metabolism control and fermentation optimization process. The method uses Aspergillus glaucus HB1-19 with the conservation number of CCTCC M 206022 as production strain and adds nickel chloride, cobalt chloride and cobalt nitrate hexahydrate in two different fermentation mediums to increase the yield of griseoviridin A, wherein the peak production rate of griseoviridin A in a fermentation medium A can reach 24.3 mg / l, which is increased by 67%, compared with the control group without metal ions; and the yield in a fermentation medium B can reach 81.1 mg / l, which is increased by 45%, compared with the control group without metal ions. The method of the invention has important significance in the fermentation research of Aspergillus glaucus HB1-19 and the metabolism control research of griseoviridin A.

The invention discloses an animal manure leavening agent and a bio-organic fertilizer for improving saline-alkali soil. The animal manure leavening agent is prepared from aspergillus glaucus, blumeria flava, trichothecium roseum, lactobacillus rhamnosus, saccharomyces cerevisiae and bacillus megatherium. The preparation process of the bio-organic fertilizer comprises the following steps: 1) adding the animal excrement and urine leavening agent, the molasses and the animal excrement and urine together, stirring and uniformly mixing; and 2) carrying out aeration fermentation on the mixed materials for more than 15 days at the temperature of 10-30 DEG C to obtain the bio-organic fertilizer. When the saline-alkali soil is improved, the bio-organic fertilizer is applied to the saline-alkali soil as a base fertilizer according to the application amount of 15t-75t / hm < 2 >. Animal excrement and urine can be fermented into the bio-organic fertilizer, the fertilizer is applied to saline-alkali soil, the salt content and the pH value of the saline-alkali soil can be reduced, the soil structure and the ecological environment of the saline-alkali soil are improved, crop growth is promoted, and the crop yield is increased.

The invention discloses a preparation method of Jinjunmei Fu eurotium cristatum tea, wherein the method comprises the steps: step 2, preparing a fermenting agent: separating out aspergillus glaucus from fuzhuan tea, and preparing to obtain the solid pure strain fermenting agent; step 3, steaming and pile fermentation of Jinjunmei: steaming the prepared Jinjunmei at high temperature, transferring into a pile fermentation bin, and carrying out pile fermentation; step 4, after steaming, adding the solid pure strain fermenting agent immediately, inoculating, adding a tea juice, then placing the inoculated Jinjunmei in a fungus growing culture room, and carrying out fungus growing culture; and step 5, rolling shaping, adding the fermented tea leaves into a small rolling machine, making the tealeaves into a brick shape by a hot rolling way, and directly packaging the dried loose tea. The invention relates to the technical field of processing of Jinjunmei Fu eurotium cristatum tea. The fungus growing time is shortened, the sundry fungus pollution rate can be reduced in the fungus growing process of the loose tea, the problems that conventional loose tea is hard in fungus growing and easyin bacterial growth can be solved, the fungus growing rate of the loose tea reaches up to 100%, and the tea leaves is widespread in eurotium cristatum on the surface and the granules are plump.

The invention discloses a bio-organic fertilizer capable of improving saline-alkali soil. The bio-organic fertilizer is mainly prepared from raw materials consisting of a complex microbial inoculant,animal waste and molasses. The complex microbial inoculant comprises aspergillus glaucus, tarlaromyces flavus, trichothecium roseum, trichoderma koningii and verticillium chlamydosporium. The bio-organic fertilizer is prepared by uniformly mixing the main raw materials, then adjusting the water content of the mixed material to 30-80%, and carrying out facultative fermentation at a temperature higher than 10DEG C for 10-60 days. The bio-organic fertilizer can promote the growth of crops planted in saline-alkali soil, reduce the salt content of the saline-alkali soil, and improve the soil physicochemical properties and ecological environment of the saline-alkali soil.

The invention provides a microbial soilimprover containing furnace ash and application thereof. The microbial soil improver is prepared by mixing complex microbial inoculants, molasses, urea, wheat middling and the furnace ash. The complex microbial inoculants are composed of aspergillus glaucus, tarlaromyces flavus, trichothecium roseum, trichoderma harzianum, verticillium chlamydosporium, lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis. The microbial soil improver is prepared by mixing the following components in parts by weight: 0.1-5 parts of the complex microbial inoculants, 2-5 parts of the molasses, 1-5 parts of the urea, 2-5 parts of the wheat middling and 75-95 parts of the furnace ash. The invention further provides application of the microbial soil improver. 20-6000 kg of the microbial soil improver is applied to per mu of soil before crops are planted in the soil.

The invention discloses a pine needle soft feed capable of increasing the fish culturing yield and a preparation method of the pine needle soft feed. The pine needle soft feed consists of the following raw materials in parts by weight: 50-70 parts of fresh pine needles, 18-22 parts of cotton seed meal, 1-2 parts of semen cuscutae powder, 1-2 parts of fleeceflower root powder, 0.3-0.5 part of aspergillus glaucus bacterial liquid, 3-5 parts of puffing sesamol, 2-3 parts of yam flour, 0.5-0.8 part of candida utilis lodd. et v. rij bacterial liquid, 0.2-0.3 part of chicken powder, 0.1-0.2 part of ox bone meal, and 0.3-0.5 part of edible gelatine. The feed disclosed by the invention is soft in nature and good in palatability, has full-bodied aromatic odor, can promote the rapid growth and weight increment of fish, shortens the culture cycle, reduces the disease of the fish, increases the survival rate of the fish, and improves the nutrition and the quality of fish; the utilization rate of the feed is increased, and finally, the breeding yield is increased.

The invention discloses an off-ground pile fermentation technology for fermented tea. The off-ground pile fermentation technology for the fermented tea comprises the following specific steps of: making a ground early-stage preparation, carrying out processing that a 30*30 cm vent hole is reserved on the bottom in every 10 meters in a workshop place for preparing the fermented tea, evenly arrangingcement piles on the ground of the place, and placing a wood plate on the top parts of the cement piles; and after 20% of moisture is sprinkled into sunning raw tea, carrying out pile fermentation onthe wood plate, overturning for one time in every three days, continuously overturning for three times, and adding 20% of moisture in sequence according to a situation while overturning is carried outto enable a water content of a pile is 40%. The invention aims to provide a reasonable off-ground pile fermentation technology capable of avoiding insufficient situations, including insanitation, water accumulation, no circulation of bottom layer air, breeding of putrefying bacteria including aspergillus glaucus and the like and also capable of avoiding an insufficient situation that big-pile fermentation can not be carried out by a small box, and moisture flowing caused by excessive pile tide water on the wood plate for the first time can be avoided. According to the fermentation technology,the problems of uneven moisture, uneven dampness and heat and excessive aspergillus niger can be avoided.

The invention discloses Fu Tea fungus growing processing technology of Dancong Tea, and relates to the field of tea processing. The processing technology comprises the following steps: preparing tea juice strain of Aspergillus glaucus, processing raw materials of Dancong Tea, carrying out steaming, inoculating and fungus growing, carrying out drying and carrying out packaging. The culture medium comprises the following components: 20-50 parts of soluble starch, 2-6 parts of peptone, 1-6 parts of yeast extract, 0.5-10 parts of monosodium glutamate, 0.01-1 part of magnesium sulfate heptahydrate,1-3 parts of potato powder, 1-3 parts of malt powder, 20-50 parts of glucose, 10-30 parts of Czapek's agar, 15-25 parts of KH2(subscript)PO4(subscript) and 10-15 parts of MgSO4(subscript). The Fu Teafungus growing processing technology of Dancong Tea solves the problems that loose tea is difficult to grow fungi and prone to breeding miscellaneous bacteria at present. After processing, the fungiare decomposed into various amino acids, giving the tea soup nutrients and fresh mouthfeel; and yeast can produce effective nutrient substances and substances with health care effects on the human body, inhibit the production of metabolic substances of food pathogenic bacteria and spoilage bacteria and improve the biological activity of various substances in tea leaves.

The invention provides a processing method of a big leaf 'post-fermented tea' dark green tea. The processing method is characterized by comprising the following steps: big leaf fresh leaf picking, water removing, rolling, drying, crude tea drying, classifying and bunching, sterilizing, inoculating, pile fermenting, turning and cooling, air drying and alcoholizing, vapor-pressing, airing, ageing, finished dark green tea; the inoculating refers to access eurotiaceae, and the eurotiaceae is the mixed thallus of aspergillus niger, rhizopus delemar, penicillium sp., aspergillus glaucus and brettanomyces intermedius; the mixed thallus is prepared from the following steps: every strain is subjected to liquid culture, is dispersed into hyphal bodies of 0.2-0.4 cm or so, suspended until 107-108 hyphal bodies or somatic cells are formed per milliliter with the volume ratio of 3: 1: 1: 8: 3, and mixed uniformly.

The invention discloses pine needle soft feed for promoting fry growth and a preparation method thereof. The pine needle soft feed comprises, by weight, 50-70 parts of fresh pine needle, 15-20 parts of soybean meal, 5-8 parts of shrimp meal, 0.2-0.3 parts of table salt, 0.3-0.5 parts of an aspergillus glaucus bacterial liquid, 0.4-0.8 parts of haw powder, 1.2-1.6 parts of powder of root of kudzu vine, 0.5-0.8 parts of a candida utilis liquid, 0.1-0.2 parts of cream, 0.1-0.2 parts of carrot extract and 0.3-0.5 parts of edible gelatin. The pine needle soft feed is soft, has good palatability and a thick fragrant smell, can promote fry growth and weight increasing, can improve a fry survival rate, can reduce fry diseases and death, can improve a feed utilization rate and can reduce a feed coefficient.

The present invention discloses a fish flesh preservation period extending pine leaf soft feed and a preparation method thereof. The fish flesh preservation period extending pine leaf soft feed is composed of the following raw materials in parts by weight: fresh pine leaves 50-70 parts, soybean curd dregs 17-22 parts, green tea dregs 3-5 parts, malt powder 2-4 parts, aspergillus glaucus liquid 0.3-0.5 part, common vladimiria root powder 0.1-0.2 part, red jujube powder 0.4-0.5 part, candida utilis liquid 0.5-0.8 part, steamed egg custard 0.1-0.2 part, fermented soybeans 0.4-0.6 part, and edible gelatin 0.3-0.5 part. The feed is soft in texture, good in palatability, and rich in aroma, and can improve antioxidant capacity of fish itself, reduces oxidation decomposition of amino acids and fat, accelerate metabolic waste discharge, extend the shelf life of fish flesh, and improve fish flesh and fish soup delicious degrees.

The invention provides a novel culture medium using marine aspergiolide glaucus to manufacture a novel antineoplastic active compound. Per liter of the novel culture medium comprises 20 to 100g of tragantine, 10 to 100g of cane sugar, 20 to 100g of maltose, 0.5 to 6g of peptone, 0.3 to 6g of yeast extract, 0.2 to 4g of soybean flour, 0.5 to 10g of sodium glutamate, 0.01 to 1g of heptahydrate magnesium sulfate, 0.01 to 2g of monopotassium phosphate and the balance being artificial seawater. A method for preparing the novel culture medium is also provided. The novel culture medium using the marine aspergiolide glaucus to manufacture the novel antineoplastic active compound has the advantages of easily received raw materials, low cost and simple preparing method, can obviously improve the yield of griseoviridin A produced and fermented by the marine aspergiolide glaucus HB1-19, improve the yield of the griseoviridin A from the prior 15mg / L to 71mg / L, is advantageous to industrial production in large scale, and has significance for the fermentation investigation of the marine aspergiolide glaucus HB1-19 and the medicine and pharmacology investigation of the griseoviridin A.

The invention relates to a preparation method of Pu'er tea fermentation seed-starter, belonging to the Pu'er tea processing technical field. The preparation method comprises: processing ground mass, inoculating, cultivating and freeze-drying; concretely: a: crushing the tea-leaf ground mass into tea powder of 40mesh; sterilizing for 25min in a process retort at 0.1MPa and 121degree; natural cooling to room temperature; b: processing Aspergillus niger, Aspergillus glaucus and common penicillium into a PDA liquid medium suspension liquid with spore number ratio of 1:1:1 using conventional method and controlling the concentration of the zymogen being 10<5>-10<7>CFU / g after inoculating and controlling the ratio of tea leaf to PDA liquid culture being 4:1; c: inoculating on the PDA culture medium at 30degree for 40h; d: freeze vacuum drying the cultured seed-starter germ at -18degree until the water content is 8-10%, therefore the seed-starter is obtained. The preparation method has features of simple preparation method, low cost, convenient use and is favourable to increase the Pu'er tea quality and especially suitable for large-scale Pu'er tea production.

The invention discloses a fungus growing processing technique on osmanthus oolong tea and Fu tea, comprising the following steps: preparing tea juice strains with aspergillus glaucus, treating raw materials of osmanthus oolong tea, steam inoculating, fungus growing, drying and packaging. The medium Including the following components: 20-30 parts of yeast extract, 1-3 parts of potato powder, 1-3 parts of malt powder, 20-50 parts of glucose, 10-30 parts of Czapek agar, 15-25 parts of K2HPO4 solution and 10-15 parts of MgSO4 solution. The starter culture medium is obtained by inoculating on a medium with aspergillus glaucus following high temperature steam sterilization. The present invention relates to the technical field of tea processing. The fungus growing processing technique on osmanthus oolong tea and Fu tea has good digestion promotion, blood fat reduction, fat dissolution, regulation of saccharometabolism, promotion of gastrointestinal movement, weight reduction and inhibition effect on bacteria, saccharomycetes, mycetes and actinomycetes, and can suppress generation of metabolites of food pathogenic microbes and spoilage organisms, solves the problems of difficult fungus growing and easy breeding of mixed bacteria in loose tea in the prior art.

The invention discloses a pine needle soft feed for improving the nutritional quality of fish and a preparation method thereof. The soft feed is prepared from the following raw materials in parts by weight: 50-70 parts of fresh pine needles, 16-20 parts of barley flour, 7-9 parts of chicken blood powder, 2-3 parts of apple leaf powder, 0.3-0.5 part of aspergillus glaucus bacterial liquid, 2-3 parts of spinach leaf powder, 1-2 parts of molasses, 0.5-0.8 part of candida utilis bacterial liquid, 0.1-0.2 part of patchouli oil, 0.2-0.3 part of duck bone extract and 0.3-0.5 part of edible gelatin. The feed has soft texture, excellent palatability and rich fragrant smell, and can be used for reducing oxygenolysis of amino acid and fat in a fish body, reducing accumulation of harmful substances in the body, accelerating discharge of metabolic waste, improving the nutritional quality of fish and improving the tasty degree of fish and fish soup.

Polymeric articles such as fibres and foams having incorporated therein a chemical compound which has antifungal activity against fungi of the groups Aspergillus glaucus and / or A. restrictus are useful in controlling house dust mites and bedmites Dermatophagoides spp.

The invention discloses a preparation method of keemun black tea Fuzhuan scented tea. The preparation method comprises the following steps of step II, preparing a fermenting agent: separating Fuzhuantea to obtain aspergillus glaucus, preparing a solid pure-strain fermenting agent, and preparing a tea extract; step III, performing withering; step IV, performing rocking of green leaves until the leaf margins change red, and flower and fruit fragrance is distributed; step V, performing rolling and shaping, wherein at the rolling temperature of 20 DEG C, the relative humidity of withered leaves is 70%, and the adding quantity is preferable when the bucket is naturally full of leaves; step VI, performing air-steaming inoculating and performing golden cleistothecium production: performing air steaming on the tea to which the tea extract is added, after air steaming, immediately adding the solid pure-strain fermenting agent, performing inoculating, placing the inoculated keemun black tea ina golden cleistothecium production culturing chamber, and performing golden cleistothecium production culturing; and step VII, performing drying, and performing packing. The invention relates to the technical field of processing of the keemun black tea Fuzhuan scented tea. The golden cleistothecium production time is shortened, the pollution rate of the tea in bulk by infectious microbes in the golden cleistothecium production process can be reduced, and the problems that at current, the golden cleistothecium production of the tea in bulk is difficult, and infectious microbes are easy to breed, are solved; and besides, the golden cleistothecium production rate of the tea in bulk achieves 100%, golden cleistothecium is formed on the surfaces of tea leaves generally, and the granules are plump.

The invention belongs to the technical field of organic solid waste treatment, and particularly relates to a biological drying method for organic solid waste. The method comprises the following steps: mixing the organic solid waste with a bulking agent and a pH conditioner to obtain a first mixed material; inoculating a microbial agent into the first mixed material to obtain a second mixed material; the microbial agent comprises pichia pastoris, lactobacillus plantarum, geotrichum candidum and aspergillus glaucus; the second mixed material is placed in a closed biological drying reactor to be subjected to biological drying for 7-12 days, and intermittent ventilation, intermittent stirring, intermittent air exhaust and waste heat recovery are carried out in the biological drying process. According to the method, the problems of long time consumption, high energy consumption, large occupied area and heavy odor of a traditional drying and biological fermentation method are solved, reduction is obvious while drying is carried out, a foundation is laid for later recycling, and the method is a rapid and effective method for achieving harmlessness and recycling of organic solid waste.

The invention discloses pine needle soft forage for improving a fry survival rate and a preparation method thereof. The pine needle soft forage is prepared from 50-70 parts by weight of fresh pine needles, 15-18 parts by weight of cotton seed meal, 0.6-0.8 parts by weight of Sedum sarmentosum Bunge powder, 0.4-0.6 parts by weight of rhizoma atractylodis powder, 0.3-0.5 parts by weight of aspergillus glaucus liquid, 0.1-0.2 parts by weight of Chinese prickly ash seed powder, 1-2 parts by weight of mung bean flour, 1-2 parts by weight of a candida utilis liquid, 3-4 parts by weight of expanded rice bran, 0.2-0.3 parts by weight of an onion juice and 0.3-0.5 parts by weight of edible gelatin. The pine needle soft forage is soft, has a good taste and a thick fragrance, can improve fry immunity, can reduce fish diseases, can improve a fry survival rate, can promote fry growth and weight increasing, can promote fry growth and weight increasing, can shorten a culture period and can improve a culture yield.

The invention discloses a method for increasing the yield of griseoviridin A by adding metal ions, which belongs to the technical field of metabolic regulation and optimization of fermentation process. The present invention utilizes the marine filamentous fungus Aspergillus glaucus HB1-19 (Aspergillus glaucus HB1-19) CCTCC M 206022 as the production strain, by adding nickel chloride, cobalt chloride and cobalt nitrate hexahydrate in two different fermentation media To increase the production of griseovirmycin A. Among them, the highest yield in fermentation medium A can reach 24.3mg / l, which is a 67% increase in the control group without adding metal ions; the highest yield in fermentation medium B can reach 81.1mg / l, which is without adding metal ions The control group improved by 45%. The invention is of great significance for the fermentation research of the marine fungus Aspergillus cinerea HB1-19 and the research on the metabolism regulation of griseovirmycin A.

A method for improving the yield of viridogrisein A by adding a promoter belongs to the technology field of the metabolism regulation optimized fermentation process. The method utilizes marine filamentous fungus Aspergillus glaucus HB1-19 of CCTCC M 206022 as a production strain, simvastatin, phthalic acid, citric acid or short-chain fatty acid, such as acetic acid, propionic acid, malonic acid are independently added in a fermentation culture medium as the promoter, or the promoters are mixedly added for promoting the synthesis efficiency for producing the viridogrisein A. The final yield can achieve 36.2mg / l which is 2.84 times of the original level. The method has important significance for researching the pharmacology and pharmacodynamics of the viridogrisein A.

The invention discloses a soft pine needle feed capable of accelerating the protein accumulation of fries and a preparation method of the soft pine needle feed. The soft pine needle feed consists of the following raw materials in parts by weight: 50-70 parts of fresh pine needles, 18-24 parts of soybean meal, 4-8 parts of blood powder, 0.5-0.6 part of cinnamomi cortex pulveratus, 0.3-0.5 part of aspergillus glaucus liquid, 2-3 parts of common curculigo leaf meal, 2-3 parts of soy sauce, 0.5-0.8 part of candida utilis liquid, 0.3-0.5 part of a pork extract, 0.2-0.3 part of chili sauce, and 0.3-0.5 part of edible gelatine. The feed disclosed by the invention is soft, is good in palatability, has full-bodied aromatic odor, can improve the utilization efficiency of the proteins by the fries, and accelerates the absorption, the transformation and the accumulation of the proteins in bodies, promotes the fries to rapidly grow and rapidly gain weight, and increases the survival rate of the fries.